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, K. ∞n
STUDIESONCUCUMBERGREENMOTTLEMOSAIC
VIRUSINJAPAN*
1
NOUYE
,T.,1NOUYE,N.,ASATANI,M.andMITSUHATA,K.
1
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INTRODUCTION
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t
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r回 w
i
n
g
.
1
TABLE3
Seed
l
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n
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n
f
e
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t
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o
nt
h
r
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g
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r
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x
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t
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a
v
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s
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x
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o
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l
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a
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h
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n
a
c
t
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t
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o
nb
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e
t
h
y
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r
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m
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d
eo
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A
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n
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np
l
a
n
tt
i
s
s
u
e
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r
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e
d
i
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o
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l(
c
f
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b
l
e9
)
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e
f
t :D. stramoniuml
e
a
fi
n
o
c
n
l
a
t
e
dw
i
t
hCGMMVf
u
m
i
g
a
t
e
da
tt
h
ed
o
s
a
g
eo
f55
g
/
m
3f
o
r48h
口u
r
s
.
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i
g
h
t:D.stramonium1
白
, fi
n
∞u
l
a
t
e
dw
i
t
hCGMMV f
u
m
i
g
a
t
e
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tt
h
ed
ω
a
g
eo
f
3f
480g
/m
o
r48h
o
u
r
s
.
R
i
g
h
th
a
l
f
l
e
a
v
e
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e
r
ei
n
o
c
u
l
a
t
e
dw
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t
hu
n
t
r
e
a
t
e
dc
o
n
t
r
o
l
.
(
8
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romt
h
el
e
f
tt
ot
h
er
i
g
h
t
,N
.g
l
u
t
i
n
o
s
al
e
a
fi
n
o
c
u
l
a
t
e
dw
i
t
h TMV f
u
m
i
g
a
t
e
da
tt
h
e
B,
d
関 a
g
eo
f0,160,320,a
n
d640g/m
'
r
白 戸c
t
i
v
e
l
y
.
t
r
e
a
t
e
da
tf
o
u
rd
o
s
a
g
e
s,55,110
,160,and480g/mB,for24or48hours. Table
10 shows t
h
er
e
s
u
l
t
so
b
t
a
i
n
e
d
. A
l
lt
h
ed
o
s
a
g
e
swere s
u
f
f
i
c
i
e
n
tt
oi
n
a
c
t
i
v
a
t
e
CGMMVa
f
t
e
r48hoursa
sb
i
o
a
s
s
a
y
e
doncucumbers
e
e
d
l
i
n
g
s
.
24hourst
r
e
a
t
,however,completeinactivation could notbe obtained at the minimum
ment
dosageo
f55g/m3
. Althought
h
r
e
ed
o
u
b
t
f
u
ll
o
c
a
ll
e
s
i
o
n
s wereproducedon3
h
a
l
f
l
e
a
v
e
so
fD
aturastramoniumoninoculation with the 阻 p extract of the
3f
i
n
f
e
c
t
e
dgreenl
e
a
v
e
st
r
e
a
t
e
da
t160g/m
o
r48hours
,theymighthavel
i
t
t
l
e
,i
f
o
n
T
.I
n
o
u
y
e
,:
N
.I
n
o
t
i
y
'
e
,'
M
.A
回 匂n
i& K
.M
i
t
s
u
h
a
t
a
:
62
TABLE10
I
n
a
c
t
L
v
a
t
i
n
ge
f
f
e
c
to
fmethylb
r
o
m
i
d
ea
g
a
i
n
s
tCGMMVi
ni
n
f
e
c
t
e
dcucumber
t
i
s
s
u
e
sb
u
r
i
e
di
ns
o
i
li
ns
e
a
l
e
dp
l
a
s
t
i
cc
o
n
t
a
i
n
e
r(
2
)
T
i
m
eo
ft
r
伺 t
m
e
n
t(
h
r
.
)
3
)
D
o
s
a
g
e(
g
/
m
2
4
55 1
1
0 1
回
B
i
関 s
s
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e
do
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1
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c
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0
。
。
Ma
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. 柿 N
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. Maximumt
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emored
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sduringsummer. Undert
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∞lercondjtionsinautumn,
however
,theminimumd田 ageofmethylbromideforthecompleteinactivationo
f
6
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CGMMVi
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a
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1
)
. Also,TMVcouldnot
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40g/m f
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.
beanyl
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da
t6
TABLE11
I
n
a
c
t
i
v
a
t
i
n
ge
f
f
e
c
to
fmethylb
r
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3
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3
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f
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nI
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, intheformerexperimentsmadebyusingtheclosedcontainers,methyl
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n
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,twoex戸 rimentswerecarriedoutunder
t
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nt
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texperimenti
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t
r
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制 民p
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ぺfor48hours. CGMMV
i
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h
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a
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n
c
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m
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rG
r
63
TABLE1
2
I
n
a
c
t
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v
a
t
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n
ge
f
f
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e
t
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ns
o
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ns
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b
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nf
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e
l
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nsummer
2
日 瑚g
eg/m
t
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o
no
ft
h
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t
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gm
a
t
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r
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L∞a
1
8
36
54
凸UAUAUnUAU
AVAVAUAUAU
0
.
6m
0.2 m
0.3 m
O.Olm
0.2 m
0.3 m
。
V0000
0.3 m
B
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h
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e
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f
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r
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m
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. F
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r48 h
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T
e
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1
9
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g
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. Maximumt
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e
; 31
:A
v
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r
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m
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四; 2
8
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2
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戸r
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f
.
配t
e
dcucumberl
e
a
v
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swereb
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r
i
e
da
tt
h
ed
i
s
t
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n
c
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f30and60cmfrom
也en
但 z
l
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,buttheotherconditionssuchastheburyingdepth
,
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e
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戸r
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,CG
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18g/m'
回
4
. Mor
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i
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/Bromide
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t
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e
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e
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∞ntainers,1/500dilutionsoftheextractswerepreparedandexaminedelectron
配 o
p
i
回 1
1
y
. Ther
a
t
i
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ft
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o
r
tp
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l
白 田e
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.
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1
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. Ther
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r
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h
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白 m伺 s
u
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t
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,110,160,and480g
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.
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. DISCUSSION
o
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o
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n1935
∞untri,白 inEuro戸 (1,3,11,16,18,21,23,24,26,32). Otherstrainsof
,sucha
scucumberaucubam
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