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1- Abbott W.S. 1925. A method of computing the effectiveness of an insecticide. Journal of Economic
Entomology, 18: 265-267.
2- Ahn Y.J., Kwon M., Park H.M. and Han C.K. 1997. Potent insecticidal activity of Ginkgo biloba-derived
trilactone terpenes against Nilaparvata lugens. ACS Symposium Series, 658: 90-105.
3- Beers E. H., Riedl H. and Dunley J. E. 1998. Resistance to abamectin and reversion to susceptibility to
fenbutatin oxide in spider mite (Acari: Tetranychidae) populations in the Pacific Northwest. Journal of
Economic Entomology, 91: 352-360.
4- Chen S.X., Wu L., Yang X.M., Jiang X.G., Li L.G., Zhang R.X., Xia L. and Shao S.H. 2007.
Comparative molluscicidal action of extract of Ginko biloba sarcotesta, arecoline and niclosamide on
snail hosts of Schistosoma japonicum. Pesticide Biochemistry and Physiology, 89: 237-241.
5- Choi Y.H., Choi H.K., Peltenburg-Looman A.M.G., Lefeber A.W.M. and Verpoorte R. 2004.
Quantitative analysis of ginkgolic acids from Ginkgo leaves and products using 1H-NMR.
Phytochemical Analysis, 15: 325-330.
6- Codex online data base. 2011. Pesticides residues in food and feed. Available at
http://www.codexalimentarius.net/pestres/data/index.html. (Visited 16 December 2011.)
7- Desneux N., Decourtye A. and Delpuech J. M. 2007. The sublethal effects of pesticides on beneficial
arthropods. Annual Review of Entomology, 52: 81-106.
8- EPA. 2011. Pesticides and Food: What the Pesticide Residue Limits are on Food? Available at
http://www.epa.gov/pesticides/food/viewtols.htm (Visited 16 December 2011).
9- Gertz H. J. and Kiefer M. 2004. Review about Ginkgo biloba special extract EGB 761 (Ginkgo). Current
Pharmaceutical Design, 10: 261-264.
10- Huh H., Staba E.J. 1992.The botany and chemistry of Ginkgo biloba L. Journal of Herbs, Spices and
Medicinal Plants, 1:91–124.
11- Jacobs B.P. and Browner W.S. 2000. Ginkgo biloba: a living fossil. American Journal of the Medical
Sciences, 108 : 341-342.
12- Jacobson M. 1990. Review of neem research in the United States. In: J. C. Locke and R. H.
Lawson(Eds.), Proceedings of a Workshop on Neem’s Potential in Pest Management Programmes, CRC
Press Inc., Maryland .
13- Lichtblau D., Berger J.M. and Nakanishi K. 2002. Efficient extraction of ginkgolides and bilobalide from
Ginkgo biloba leaves. The Journal of Natural Products, 65: 1501-1504.
14- Lindquist R. L., Adams A. J., Hall F. B. and Adams I.H.H. 1990. Laboratory and greenhouse evaluations
of margosan-o against bifenthrin-resistant and susceptible greenhouse whiteflies, Trialeurodes
vaporariorum (Homoptera: Aleyrodidae). In J. C. Locke and R. H. Lawson [eds.], Proceeding,
Workshop on neem’s potential in pest management programs, USDA-ARS, Beltsville, MD. ARS 86: 2328.
15- Matsumoto T. and Sei T. 1987. Antifeedant activities of Ginkgo biloba L. components against the larva
of Pieris rapae Agricultural Biology and Chemistry, 51: 249-250.
16- Moosavi M. 1996. Biological control. Jahad Daneshgahi press, Mashhad, 486 pp. [In Persian with
English summary]
17- Potter Precision Spray Tower. 2000. Burkard Manufacturing Co. Ltd., Available at
http://www.burkard.co.uk/index.htm (Visited 20 June 2011).
18- Sakuma M. 1998. Probit analysis of preference data. Applied Entomology and Zoology, 33: 339-347.
19- SAS Institute Inc. 2001. SAS Campus Drive, Cary, North Carolina 27513.
20- Sticher O. 1994. Biochemical, pharmaceutical and medical perspectives of Ginkgo preparations. In: New
Drug Development from Herbal Medicines in Neuropsychopharmacology. Symposium of the XIXth
CINP Congress, Washington, DC.
315 .... & #!'* +,/4 + 7,:, +;4
21- Sticher O. 1993. Quality of Ginkgo preparations. Planta Medica, 59:2–11.
22- Sukumar K., Perich M. N. and Boobar L. R. 1991. Botanical derivatives in mosquito control: a review.
Journal of American Mosquito Control Association, 7: 210-237.
23- Sun L., Dong H., Guo C., Qian J., Sun J., Ma L. and Zhu C. 2006. Larvicidal activity of extract of
Ginkgo biloba exocarp for three different strains of Culex pipiens pallens, Journal of Medical
Entomology, 43: 258-261.
24- Swain T. 1977. Secondary compounds as protective agents. Annual Review of Plant Physiology, 28:
479-501.
25- Tolidaru Pharmaceutical Company. 2010. Ginko TD. Available at http://www.toliddaru.ir/ index-6.html
(Visited 20 June 2011).
26- Van Beek T.A. 2002. Chemical analysis of Ginkgo biloba leaves and extracts. Journal of
Chromatography, 967: 21–55.
27- Van Beek T.A. and Montorob P. 2009. Chemical analysis and quality control of Ginkgo biloba leaves,
extracts, and phytopharmaceuticals, Journal of Chromatography, 1216: 2002-2032.
28- Whalon M. E. Mota-Sanchez D. and Hollingworth R. M. 2008. Global pesticide resistance in arthropods.
CABI Press, 208pp.
29- Wink M. 1993. Production and application of phytochemicals from an agricultural perspective, pp 171213. In T. A. van Beek and H. Breteler [eds.], Phytochemistry and agriculture oxford. Claredon Press,
Oxford, United Kingdom.
30- Weigao P., Peng L., Ruobin F., Ping G., Zhangfu L., Feiyi X., Haibo X. and Shigui L. 2006. Acaricidal
activity against Panonychus citri of a ginkgolic acid from the external seed coat of Ginkgo biloba, Pest
Management Science, 62: 283-287.
31- Xu Y. X., Huang S. L., Cheng X. F. and Gong L.B. 2003. Molluscicidal effects of extracts of ginkgo
biloba’s peel on Oncan elania hupensis. China Schistosomiasis Control, 15:61-63.