...

圧力生理学から見た高水圧による酵母生理機能の

by user

on
Category: Documents
27

views

Report

Comments

Transcript

圧力生理学から見た高水圧による酵母生理機能の
3 20
¹½d[º»
·»e¼´_½
!"#$ %&' (%)
2* <$ w@n(I@ 25 MPa '#WX4$ 4&1
* +%,-./01234
15’“KMo+K1!NWX<!"#.e2* $%o
256$ 7898%:* (%) ;"&
+$ :2&1&
M!".p”A$ {{|}Mq
3<=>?@7898A"$ BCDEFG
nrz‘st#8•;X8Mp”K1WX<!"#
H@.&I"&* $ J%K#<LM
.* $%o+@RuMvwW4xoK‘‹
@$ !NM"#$%%.O8* PI&M'%(Q
$ 7M$ yˆ#–ŽzM{{|}lmAe
@$ -#)*J%1M$%
'R C;* PMS;
2M:*
@T.MAI"U @%VM!"#4C+,4C;
78M{{|}lm@Š#{N$ ((IMt—˜™
M&U #4CWX@BY#%-.M/@T&
) |•8C;* šV}~M=›|œ$ €Y?
0P#42* 7MZ12342M$ (%[
™{|G‚ƒM 4-|žŸ„O$ …{O$ |G{™|
(Piezophysiology)) :* \4#5RA6]78 Sac-
G ¡$ 74C$%o+;"xaM.;_¢
charomyces cerevisiae @$ P"4294;^:<
{{|}_‡„MWX@AW* 74C!NM"#$
M#;<._`:*
Tat2 $n#!n†.* P"42ƒ@;X81R
1. !"#$%&'
u#t‡‘8M$ uˆ#Ž£¤<* rWn$ uˆ
:=$ %>?4278Qa.3$ bc.d
dMŽx‰#zM{{|}lmR.MA* $
#@AWe2* 78$%>?4.3fBghC4C;
%K# &M@{{|}lm¥#T&S;
2* <>?D 6 EijZ42PI&3$ 20k30 MPa
:M&148.;* @.¦{{|}A'.M&U
l200k300 J%$ F& 2,000k3,000 m GHm M(I6]
Š§.‹@.;MA$ 20 N
MtuvOMŒ@t^
Jn(IoN.eC 2* FACS pK&3$ PM (I
;:n$ ŽM¨#;1‘.;.VM’$ l
Lq G1 qMA4C;PO&e2* ;r $ >?
m¥@“dMZ1”•d42M&148.;*
s# 20 N
MtuvOw7MPQR$ S
.Vxrxr
2. &'()%*+,-"./01
Ty#ZU#V8C$%>?4Cz2* <{{|}
j#$%KWX<©„M–—z2* 7M56$ (
2~€Mz(I@WX42MA* YZ<>?‚
IMª«—¡™F¬$%WXw Tat2 M˜#™_.P
Ha2 Mƒ @[1\838.;* Q„M…]^†
O&eC 2* š382!)M©„@$ ›œ. 4 G
a23$ trp1 leu2 lys2 his3 ura3 ade2 $ {{|}_
high-p
pressure growth) 1, HPG
­#t
‘8$ 783 HPG (h
‡„Ae2* `aˆM{{|}_‡b_‡M„
2, HPG3 ®!¯ HPG4 42* HPG1 M 4 ©t˜@$ ;X
<‰Cc‰2PI$ %<de lTrp῍ „m @Š.N
81 HECT †ª«—E° Rsp5 MžŸ±>=Mt
ῌ
G1 qMA4$ De lTrp „m @WXf@gˆ#‹!Œ 25
uvOM ²Ae2* Rsp5 @~œ³FG#¡RA¢¨´
MPa W2* hnM 5 NM_‡#4C@Ž.;M
BC;* Tat2 @7Mª«—tpM£MxrAe2 l¤
#$ {{|}A'.i6LMC1c#
1m* $ ¥„ 25 MPa >?< Tat2 @fw&#t
2*
p‘82* PI HPG1 „@$ Tat2 (Iu#!#¦
j#$k{{|}lmR Tat2 $n‘‹Cz
§4$ &r$%>?D1 Tat2 @tp‘8.&e2* Rsp5 M5
2 1 6]78 Saccharomyces cerevisiae M{{|}_‡„$%KWX<p”
>?p”w©$ …]^¨µ
{{|}MZUª«
Tat1 W2@ Tat2 M$n
HPG1 ©
HPG2 ©
HPG3, HPG4 ©
Bul1 »%
Doa4 »%
Ubp6 »%
Ubp14 »%
Vps27 »%
© Š
¶¬>­# 1 g/L ®
!·¸œ™u±QV
Rsp5 ª«—E° HECT ±>=MtuvO ²¹
HPG1-1 (Rsp5Pro514ºThr), HPG1-2 (Rsp5Cys517ºTyr), HPG1-3 (Rsp5Cys517ºPhe), HPG1-4 (Rsp5Ala799ºThr)
Tat2 M(I±>=MtuvO ²¹
HPG2-1 (Tat2Glu27ºPhe), HPG2-2 (Tat2Asp563ºAsn), HPG2-3 (Tat2Glu570ºLys)
¯°±
Rsp5 5#/0
²ª«—d7³
26S Ft›¬´¢^
¼ª«—µMtp#«
MVB ¶Mª«—·¸R
~|·¸¹
º»¼€~½
% 21
Bul1 Tat2
2x ŽMNK1)OP. Tat2 HPG2 3 5JUS I~QV‘ 5 Tat2 Tat2 !"#$%&'(
a_]98;H‡ˆI\ML/lmI1 ,5
) Tat2 *Hpg2 +,)
- ./)
) Rsp5 K1Q’R=.S 5 Tat2 234
* 1- HPG3 HPG4 )01
5KQTRJI1 X1 HPG1 HPG2 )
23456 Doa4, 26S 789:;<=>?
I“ŒH ‰Š Tat2 \MLUVS1 KD
Ubp 6 @A2345BCD Ubp 14 01
Rsp5 \ML)W€VmŒ X Tat2 EF)
MVB (multivesicular body) 2345 Vps27 E
=Y”•1)EVkDVy †F
FG) HI
Tat2 .CJIH
&\MLVEH,-– Tat2 . 2345
KD LA7LMNOPQ
2/~QV‘\MLZYS1 D@—˜EY"
RCS1D K1 Tat2 DmGF S1 € !N[\~ Tat2
CT UVS1WVEUE X!Y"#
.CJI1R]. ) ™Q’Rš 01)
$S1ZEQ. %S[\ML (lipid raft) )0
EV›Œ1
1
4. !"#
3. LA7LMN67^D67=Y œ5
[\ML ]M^_[`]98; *6&)
a_]98;- bc'(!)"#$)
V (DV) _VUž`
‰Š d/SKD
I
Tat1 Tat2 LA7LMN67abXc~E=Y
defAg8A[* *d/ &\ML- h+JI
*V- S. UVEQ œ5V %
1 ,-) Tat2 &\ML.iS1.jVQ
d)eŒUI1
k. HPG1 HPG2 K Tat2 \ML/l
mI !)n0KGopI1KDEQ X1 Tat2 (( ln kcat/( p)TŸ DV/RT
) k cat f¡/)_JILA7LMN/
q r 8 f ) 2 3 4 5 L A 7 L M N 6 7 Tat 1 l¢gM p N T hiŽM R £<)01
Hῌ-ATPase Pma1 ,-)\ML.i HPG1 Tat1 Tat2 ¤S1LA7LMN67 DV K1siEVQ D)t89u (TMD;
I 89.3 ¦K§ 50.8 mL/mol DbXc~Ej
transmembrane domain) :) v.\M
*k 1- LA7LMN67l¨mn) 6
L/&\MLsiwx1Vy
I¥
m Ez Tat2 )
7.c~GopS1©q1 D
2345ῌ I.A{;|)0Q
Tat2 ;
c~E=Y.LA7LMN67rs)0 R
}~
ῌsi.j1Vy
2Ž01tª«5¬>EQ1)0kD LA
€ TMD 5
%&'‚ƒ<=> SEj€
<.:)?)
7LMN67­®qu Tat1 Tat2 $ 2
@Ex a_]98;A'ƒ„B p C?.C…
vVw)01 Tat1 '(\ML
DE1D†F)01 TMD G5%&'‚
.i1
VVjUH '(2&\ML.iS
ƒ: Tat1 Pma1 a_]98;H‡ˆI
1 Tat2 KV.c~©eQ ¦ UGI
‰Š\MLsiS1 X1 Tat2 JGKAL)01.‹Œ
ƒxmnV Tat1 1.yJ. (VTat1¯VTat2), l
&\ML)
EkD  &\ML'(.

¨mn)W°zE1x (V 
Tat1 ŸV Tat2), V±
$ 1 LA7LMN67^D²$³%´E=Y
LA7LMN67 Tat1 Tat2 )67^Dœ5V.bXc~G ²$³%´=Y1.jV



1 DV
±{|)Gsi SEj€\ML01V&\ML
Tat1 (ŸVTat1 VTat1)µDVTat2 (ŸVTat2 VTat2) )0
01V}¶1
ῐ 22
¸P'd|/}¹
º}’»¼R½
Tat1 (DV
Tat1 DV Tat2) )./*+t,uv-./w.UxytF>hz
!"#
* h{)|/}~p0&€H Nhu‚1
$%&
'()*+,-./0
&2ƒ*„p…3ZQ†h‡ˆ‰Š4 ‹*+t,
uv-./w.Ux Œ-5 dŽ6‘BH.
5. ῌ ῏ ῎ ῍
/’g7&8ZQ†h9:;<4 ‹=>d
123 45678
dŽ“d./“”• –?./&—˜™@ Ap…
9:&;< =*>?@AB CDE
3š/ZQ†hB:›C4 ‹=œdd”ždD”
FA>? G&HIJKL-M
• Ÿ ¡./&43Q†E ¢4 ‹F)
NOPQR>? ST F
d%£¤“dD”• ¥¦§vG%./4¨r
U&+,-VWL-XYS5Z H./I©B;JKL4 ‹v-./w.Ux ª
9:[ \]^_%`a&bc*
M-W«- - ¬­ L4 ‹=-d“dD”•
!de"#$fQ> ghiH
v-./w.UxMN®O ¯gF)P'do|/
D& j'd%k l 2004 & 9 mn)'*Uo&
}Q°±²4³´µ¶g­·N*
hZh pq(hZrhsB
Fly UP