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Binding and enrichment of Escherichia coli spheroplasts expressing inner membrane tethered scFv
antibodies on surface immobilized antigens.
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« 2004 :¬íßâå03 George Georgiou ÒÉáÿăöʬPÁÇ0€…îÛåöĀÜãåíú¬anchored
periplasmic expression (APEx)× PNAS È+"¼Í¼Á­ºÉD\Ê>SÉD\ÆÊeÇÓ¬ihêĂòà
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Hot-spot Mimicry of a Cytokine Receptor by a Small Molecule
C. D. Thanos, W. L. DeLano, and J. A. Wells
Proc. Natl. Acad. Sci. USA, 103, 15422-15427 (2006)
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Structural Basis for Cooperative Transcription Factor Binding to the CBP Coactivator
R. N. De Guzman, N. K. Goto, H. Jane Dyson, and P. E. Wright
J. Mol. Biol., 355, 1005-1013 (2006)
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The Role of Protein Dynamics in Increasing Binding Affinity for an Engineered Protein-Protein
Interaction Established by H/D Exchange Mass Spectrometry
J. R. Horn, B. Kraybill, E. J. Petro, S. J. Coales, J. A. Morrow, Y. Hamuro, and A. A. Kossiakoff
Biochemistry, 45, 8488-8498 (2006).
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How Gene Order Is Influenced by the Biophysics of
Transcription Regulation
G. Kolesov, Z. Wunderlich, O. N. Laikova, M. S. Gelfand, and L.
A. Mirny
Proc. Natl. Acad. Sci. USA, 104, 13948-13953 (2007).
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Probing Transcription Factor Dynamics at the Single-Molecule Level in a Living Cell
J. Elf, G-W. Li, and X. S. Xie
Science 316, 1191-1194 (2007).
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No.25 (2007 October) 29
A Tunable Genetic Switch Based on RNAi and Repressor Proteins for Regulating Gene Expression in
Mammalian Cells
T. L. Deans, C. R. Cantor, and J. J. Collins
Cell 130, 363-372 (2007).
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No.25 (2007 October) 31
A
-10
B
Time (min)
0
10
20
30
40
50
60
70
80
90
-10
C
Time (min)
0
10
20
30
40
50
60
70
80
90
0.0
cal/sec
0.0
Time (min)
0
10
20
30
40
50
60
70
0.0
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2
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0
0
0
-2
-2
-2
-4
-4
Model: OneSites
Chi^2 = 13915.2
N
1.657 ±0.00684
K
4.329E6±2.432E5
H
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S
-10.28
-6
-8
-10
-12
-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Molar Ratio
-4
Model: OneSites
Chi^2 = 35839.2
N
1.949 ±0.00745
K
2.45E7 ±2.972E6
H
-1.296E4±84.28
S
-9.674
-6
-8
-10
-12
-14
-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5 5.0 5.5
Molar Ratio
-6
-8
-10
Model: OneSites
Chi^2 = 18575.9
N
1.873 ±0.00546
K
1.359E8±2.793E7
H
-1.031E4±58.95
S
2.633
-12
-0.5 0.0 0.5 1.0 1.5 2.0 2.5 3.0 3.5 4.0 4.5
Molar Ratio
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No.25 (2007 October) 32
CŸa/äøöòíöúûDifferential Scanning Calorimetry: DSCüâz»Îµ?7Ԉ&Ј&JÔ
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0.0
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20
30
40
50
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60
70
80
90
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0
10
20
30
40
50
60
70
80
90
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kcal/mole of injectant
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0
-4
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1.976 ±0.00521
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1.183E8±1.348E7
H
-1.665E4±80.10
S
-18.91
-8
-12
-16
-1
Model: OneSites
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N
1.955 ±0.0662
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5.636E4±7368
H
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S
8.848
-2
-3
-4
0
1
2
3
4
5
6
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Molar Ratio
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3
4
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