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第8章 ニホンイノシシのウイルス感染症
රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ➨ 㸶 ❶ ࢽ࣍ࣥࣀࢩࢩࡢ࢘ࣝࢫឤᰁ ⡿‶◊୕ 1࣭᭹㒊ᚿಖ 1࣭㕥ᮌ⤢Ꮚ 1࣭ᓮ༓⳯⨾ 1࣭ୗ⏣ ᐂ 1࣭๓⏣ 1 せ Ⅼ ࣀࢩࢩឤᰁࡍࡿ࢘ࣝࢫឤᰁࡢ୰࡛ࠊࣄࢺࡸ⏕⏘ື≀㸦≉ࣈࢱ㸧ᑐࡋ࡚ ၥ㢟࡞ࡿ E ᆺ⫢⅖࢘ࣝࢫࠊ㔜⇕ᛶ⾑ᑠᯈῶᑡೃ⩌࢘ࣝࢫࠊ࣮࢚࢜ࢫ࣮࢟ ࢘ࣝࢫࠊ᪥ᮏ⬻⅖࢘ࣝࢫࡢᏛㄪᰝࢆᐇࡋࠊࣄࢺࡸື≀ࡢࣜࢫࢡࡘ࠸࡚ ⪃ᐹࡋࡓࠋ Key words㸸ࣀࢩࢩࠊE ᆺ⫢⅖࢘ࣝࢫࠊ᪥ᮏ⬻⅖࢘ࣝࢫࠊ㔜⇕ᛶ⾑ᑠᯈῶᑡೃ⩌࢘ ࣝࢫࠊ࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫ 㸫ࡣࡌࡵ ࢽ࣍ࣥࣀࢩࢩ㸦Sus scrofa leucomystax㸹௨ୗࣀࢩࢩ⾲グ㸧ࡣ㞧㣗ᛶ࡛࠶ࡾࠊ ㏆ᖺ㎰స≀➼ࢆồࡵ࡚ࣄࢺࡢ⏕άᅪධࡍࡿࡼ࠺࡞ࡗ࡚ࡁࡓࠋࡲࡓࠊྂ᮶ࡼࡾࣀ ࢩࢩࡢ⫗ࡣࠊ㣗⏝ࡋ࡚ᾘ㈝ࡉࢀ࡚࠸ࡿࠋࣀࢩࢩឤᰁࡋࠊྠࣄࢺࡸࡑࡢࡢື ≀ࠊ≉ࣈࢱࡶឤᰁࡋẼࢆᘬࡁ㉳ࡇࡍ࢘ࣝࢫࡣࠊᅜෆ࡛ࡣ E ᆺ⫢⅖࢘ࣝࢫࠊ 㔜⇕ᛶ⾑ᑠᯈῶᑡೃ⩌࢘ࣝࢫࠊ࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫࠊ᪥ᮏ⬻⅖࢘ࣝࢫࠊ㇜ ࣥࣇ࢚ࣝࣥࢨ࢘ࣝࢫࠊ㇜ࣃࣝ࣎࢘ࣝࢫࠊ㇜⦾Ṫ࣭྾ჾ㞀ᐖೃ⩌࢘ࣝࢫࠊ㇜ ࢧ࣮ࢥ࢘ࣝࢫ 2 ࡞ࡀ࠶ࡿ㸦⾲㸯㸧 ࠋࡋࡋࠊࣀࢩࢩ࠾ࡅࡿឤᰁ◊✲ࡢṔྐࡣ ὸࡃࠊ◊✲ࡀ㊊ࡋ࡚࠸ࡿࡓࡵࠊ⾜ᨻࡸ⊁⊟⪅ࠊ୍⯡ᕷẸ࡞⌧ሙࣞ࣋ࣝࡲ࡛༑ศ࡞ ሗࡀఏࢃࡗ࡚࠸ࡿࡣゝ࠸㞴࠸ࠋࡑࡇ࡛ᮏㄽ࡛ࡣࠊᡃࠎࡢ᭱᪂ࡢ◊✲ᡂᯝ㸦Hara et al. In press; Takahashi et al. In press; ๓⏣ 2014; Mahmoud et al. 2011; Shimoda et al. 2013; Shimojima et al. 2011; Shimoda et al.; Ohno et al. 2009㸧ࡼࡾุ᫂ࡋࡘࡘ࠶ ࡿࠊࣀࢩࢩ࠾ࡅࡿ E ᆺ⫢⅖࢘ࣝࢫࠊ᪥ᮏ⬻⅖࢘ࣝࢫࠊ㔜⇕ᛶ⾑ᑠᯈῶᑡ ೃ⩌࢘ࣝࢫࠊ࠾ࡼࡧ࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫ㛵ࡍࡿឤᰁ≧ἣࡸឤᰁ⤒㊰ࡢ≉ᚩࢆ⾜ ᨻᢸᙜ⪅ࡸ⊁⊟⪅࡞ࡢᑐ⟇⪅ྥࡅ⥲ㄽⓗ⤂ࡋࠊࣄࢺࡸື≀ࡢឤᰁࣜࢫࢡࡘ ࠸࡚⪃ᐹࡋࠊࡲࡓឤᰁ㜵ᚚ᪉ἲࡘ࠸࡚ᥦゝࡍࡿࠋ࡞࠾ࠊᮏ◊✲࡛ࡣᢠཎ㸦࢘ࣝࢫࡢ 㑇ఏᏊ㸧᳨ฟ᳨ᰝᢠయ᳨ฟ᳨ᰝࢆ⾜ࡗࡓࠋᢠཎ᳨ᰝࡣࢧࣥࣉࣜࣥࢢࡉࢀࡓᙜࡢ࢘ ࣝࢫࡢឤᰁ᭷↓ࢆ᳨⣴࡛ࡁࡿ᳨ᰝ࡛࠶ࡿࠋ୍᪉ࠊᢠయ᳨ᰝࡣࠊ㐣ཤࡢឤᰁṔࢆ᳨⣴ࡍࡿ ࡇࡀ࡛ࡁࡿ᳨ᰝ࡛࠶ࡿࠋ ᒣཱྀᏛඹྠ⋇་Ꮫ㒊ែไᚚᏛㅮᗙ⋇་ᚤ⏕≀Ꮫศ㔝 රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ⾲㸯㸬ࣀࢩࢩ⏤᮶ࡢࣄࢺ࠾ࡼࡧື≀ឤᰁࡍࡿྍ⬟ᛶࡢ࠶ࡿせ࡞࢘ࣝࢫឤᰁ ( ᆺ⫢⅖ ( ᆺ⫢⅖ࡢᏛ୍⯡ᛶ≧ E ᆺ⫢⅖ࡣࠊE ᆺ⫢⅖࢘ࣝࢫ㸦௨ୗࠊHEV㸧ឤᰁࡼࡗ࡚ᘬࡁ㉳ࡇࡉࢀࡿே⋇ඹ ㏻ឤᰁ࡛࠶ࡿࠋⓎᒎ㏵ୖᅜ࠾࠸࡚ࡣỈࢆࡋࡓ⣅ཱྀឤᰁࡼࡾឤᰁࡍࡿ࠸ࢃࢀ࡚ ࠾ࡾࠊ௨๓ࡣࢃࡀᅜ࡛ࡣὶ⾜ᆅΏ⯟ࡋࡓࣄࢺࡀឤᰁࡍࡿࠊ㍺ධឤᰁࡋ࡚ㄆ㆑ࡉࢀ ࡚࠸ࡓࠋࡋࡋࠊ2003 ᖺ 4 ᭶රᗜ┴࡛ࢽ࣍ࣥࢪ࢝㸦Cervus Nippon㸹௨ୗࢩ࢝⾲ グ㸧ࡢ⫗ࡢ⏕㣗ࡼࡿឤᰁࡀⓎ⏕ࡋࠊࡇࢀࡀ㣗ရࡼࡿឤᰁࡢึࡵ࡚ࡢ☜ㄆ ࡞ࡗࡓ㸦Tei et al. 2003㸧 ࠋࡑࢀ௨㝆ࠊE ᆺ⫢⅖ࡣ㣗ရ፹ᛶឤᰁࡋ࡚ㄆ㆑ࡉࢀࡿࡼ ࠺࡞ࡗࡓࠋࡲࡓࠊᕷ㈍ࡉࢀ࡚࠸ࡿ㇜ࡢ⏕ࣞࣂ࣮ࡽ HEV ࡀ᳨ฟࡉࢀࡓࡇࢆࡣࡌࡵ 㸦Yazaki et al. 2003㸧 ࠊ⌧ᅾࠊ㇜⫗ࠊࣀࢩࢩ⫗ࠊࢩ࢝⫗ࡢ⏕⫗ࡢႚ㣗ࡀᅜෆ࡛ࡢࣄࢺ ࡢឤᰁ※ࡋ࡚㔜せどࡉࢀ࡚࠸ࡿࠋࡲࡓࠊE ᆺ⫢⅖࢘ࣝࢫࡣ 4 ✀㢮ࡢ㑇ఏᏊᆺࡀ Ꮡᅾࡋࠊ1 ᆺࠊ2 ᆺࡀᾏእ࡛ὶ⾜ࡋ࡚࠸ࡿࡢᑐࡋ࡚ࠊ3 ᆺࠊ4 ᆺࡣᅜෆ࡛ື≀ࡢ⫗ ࡽឤᰁࡋ࡚࠸ࡿࡇࡶ▱ࡽࢀ࡚࠸ࡿࠋ⌧ᅾࠊឤᰁࡢண㜵ཬࡧឤᰁࡢᝈ⪅ᑐࡍࡿ་ ⒪㛵ࡍࡿἲᚊ㸦௨ୗࠊ ࠕឤᰁἲࠖ 㸧ᇶ࡙ࡁࠊE ᆺ⫢⅖ࡣឤᰁἲࡢ➨ᅄ㢮ឤᰁ ᣦᐃࡉࢀ࡚࠾ࡾࠊᝈ⪅ࢆデ᩿ࡋࡓ་ᖌࡣಖᡤ┤ࡕᒆฟࡿࡇࡀ⩏ົ࡙ࡅࡽࢀ࡚࠸ ࡿࠋ ᮏ◊✲࡛ࡣࠊ᪥ᮏࡢ 3 ᆅᇦ࡛ᤕ⋓ࡉࢀࡓࣀࢩࢩ࠾ࡼࡧࢩ࢝ࡽᚓࡽࢀࡓ⾑ᾮࢆศᯒ ࡋࠊᢠయ࠾ࡼࡧᢠཎ㸦࢘ࣝࢫࡢ㑇ఏᏊ㸧ࡢ᳨ฟࡢ୧ᐇ㦂ࡼࡾࠊHEV ࡢឤᰁ≧ἣࢆ ศᯒࡋࡓࠋ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ᮦᩱ࠾ࡼࡧ᪉ἲ ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ ୰ᅜ࣭ᕞ࣭㛵ᮾᆅ᪉࡚ 2009 ᖺࡽ 2013 ᖺᤕ⋓ࡉࢀࡓࣀࢩࢩࡼࡾᚓࡽࢀࡓ ⾑Ύࢆ⏝࠸ࡓࠋࣀࢩࢩ⾑Ύࡢࢧࣥࣉࣝᩘࡣ୰ᅜᆅ᪉ 167ࠊᕞᆅ᪉ 46ࠊ㛵ᮾᆅ᪉ 152 ࡛࠶ࡗࡓࠋࡲࡓẚ㍑ᑐ㇟ࡋ࡚ࠊࣀࢩࢩ⾑Ύࢆ᥇ྲྀࡋࡓࡢྠᆅᇦ࡛࠶ࡿࠊ୰ᅜᆅ᪉ ࡛ᤕ⋓ࡉࢀࡓࢩ࢝ࡼࡾᚓࡽࢀࡓ⾑Ύࡶศᯒ⏝࠸ࡓࠋࢩ࢝⾑Ύࡢࢧࣥࣉࣝᩘࡣ 209 ࡛ ࠶ࡗࡓࠋ᳨ᰝ┠ⓗࡀ␗࡞ࡿࡓࡵࢧࣥࣉࣝᩘࡣᕪࡀ࠶ࡿࠋ +(9 ᑐࡍࡿᢠయࡢ᳨ฟ ELISA ἲࡼࡾ⾑Ύ୰ࡢᢠయࢆ᳨ฟࡋࡓࠋELISA ᢠཎࡣࠊୗ㛵ࡢ HEV ᝈ⪅ࡢ㑇ఏ Ꮚ㸦JTF-Yamagu11 ᰴ㸧ࢆᇶ N ᮎ➃㡿ᇦࢆḞᦆࡋࡓ ORF2 ࢆࢥ࣮ࢻࡍࡿ㑇ఏᏊࡣࣉ ࣐࣮ࣛYamagu11 ORF2 112F (ClaI)(5’-GTA TCG ATC ACC ATG GCT GTG GCT CCG GCC CCT-3’) Yamagu11 ORF2 660R-His (5’-GTA GAT CTT CAG TGA TGG TGA TGG TGA TGG TAC TCC CGG GTT TTA CCC A-3’)࡛ቑᖜࡋࡓࠋቑᖜࡋࡓ㑇ఏ Ꮚࡣ ClaI BglII ࡛ษ᩿ࡋࡓᚋࠊpCAGGS/MCS ࡢ ClaI BglII ࢧࢺᤄධࡋࡓࠋ ᚓࡽࢀࡓⓎ⌧ࣉࣛࢫ࣑ࢻࢆ 293T ⣽⬊࣏࢚ࣜࢳ࣑ࣞࣥࣥࢆ⏝࠸࡚ࢺࣛࣥࢫࣇ࢙ࢡࢩ ࣙࣥࡋࡓࠋࢺࣛࣥࢫࣇ࢙ࢡࢩࣙࣥ⣽⬊ࡣ RIPA ࡼࡗ࡚ 4Υ1 㛫ฎ⌮ࡋࡓᚋࠊ15000 ᅇ㌿ 4Υ30 ศ㛫㐲ᚰࡋ࡚ୖΎࢆᅇࡋࡓࠋᢳฟᢠཎࢆ 5ȣg/mεᕼ㔘ࡋࡓᚋࠊ100ȣε ࢆྛ࢙࢘ࣝ᥋✀ࡋ࡚ ELISA ࢆ⾜ࡗࡓࠋࣈࣟࢵ࢟ࣥࢢᾮ࠾ࡼࡧᢠཎᕼ㔘ᾮࡣࣈࣟࢵ ࢡ࢚࣮ࢫࢆ⏝࠸ࡓࠋ⾑Ύࡣ 1㸸100 ᕼ㔘ࡋࠊḟᢠయࡣ࣌ࣟ࢟ࢩࢱ࣮ࢮᶆ㆑ ProteinA/G ࢆ 1㸸10000 ᕼ㔘ࡋ࡚⏝࠸ࡓࠋⓎⰍࡣ Bio-Rad ࡢࣃ࣮࢜࢟ࢩࢲ࣮ࢮᇶ㉁ ࢟ࢵࢺࢆ⏝࠸ࡓࠋ +(9 㑇ఏᏊ᳨ฟ ⾑Ύࡽ QIAamp Viral RNA Mini Kit ࢆ⏝࠸࡚ RNA ࢆᢳฟࡋࠊHEV-F1 ࣉ࣐ࣛ ࣮ HEV-R2 ࣉ࣐࣮ࣛࢆ⏝࠸࡚ RT-PCR ࢆᐇࠊ᭦ RT-PCR ⏘≀ࢆࠊHEV-F2 ࣉ ࣐࣮ࣛ HEV-R1 ࣉ࣐࣮ࣛࢆ⏝࠸࡚ Nested PCR ࢆ⾜࠸ࠊ㑇ఏᏊࡢ᳨ฟࢆヨࡳࡓࠋ HEV 㑇ఏᏊࡢ᳨ฟࢆヨࡳࡓࠋ㝧ᛶࡀࢃࢀࡿࢧࣥࣉࣝᑐࡋ࡚ࡣࠊሷᇶ㓄ิࢆỴᐃࡋࠊ ᭱⤊ⓗุᐃࡍࡿࡶࠊ㑇ఏᏊᆺࡢゎᯒࢆ⾜ࡗࡓࠋ ⤖ᯝ࠾ࡼࡧ⪃ᐹ ࣀࢩࢩ࡛ࡣ୰ᅜᆅ᪉࠾࠸࡚ 75 㢌୰ 23 㢌㸦31%㸧ࠊᕞᆅ᪉࠾࠸࡚ 46 㢌୰ 10 㢌㸦22%㸧 ࠊ㛵ᮾᆅ᪉࠾࠸࡚ 152 㢌୰ 12 㢌㸦8%㸧ࠊࢩ࡛࢝ࡣ୰ᅜᆅ᪉࠾࠸࡚ 209 㢌୰ 1 㢌㸦0.5㸣㸧ࡀࠊHEV ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓ㸦⾲㸰㸧ࠋᚑࡗ࡚ࠊHEV ᑐ ࡍࡿឤᰁ⋡ࡣᆅᇦᕪࡀ࠶ࡿࡶࡢࡢࠊ3 ᆅᇦ࡛ࡣࠊࣀࢩࢩࢆឤᰁᐟࡋ࡚ HEV ࡀศ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ᕸࡋ࡚࠸ࡿࡇࡀ♧၀ࡉࢀࡓࠋࡲࡓࠊྠ୍ᆅᇦ࡛࠶ࡿ୰ᅜᆅ᪉࠾࠸࡚ࡣࠊࣀࢩࢩ ࢩ࢝ࡢ HEV ឤᰁ⋡ࡀࡁࡃ␗࡞ࡾࠊࢩ࢝ࡢឤᰁ⋡ࡣ㠀ᖖపࡗࡓࡇࡽࠊࢩ࢝ ࡘ࠸࡚ࡣࡇࢀࡲ࡛ࡢሗ࿌ྠᵝࠊHEV ࡢឤᰁࡣ㠀ᖖࡲࢀ࡛࠶ࡿࡇࡀ♧၀ࡉࢀࡓࠋ HEV 㑇ఏᏊ᳨ᰝࡼࡾࠊ࢘ࣝࢫࡢ㑇ఏᏊࡢಖ᭷ࡢ᭷↓ࢆㄪࡓࠋࣀࢩࢩ࡛ࡣ୰ ᅜᆅ᪉ࡢಶయ 167 㢌୰ 6 㢌㸦4%㸧ࡽࠊࢩ࢝࠾࠸࡚ࡣ୰ᅜᆅ᪉ࡢಶయ 201 㢌୰ 1 㢌 㸦0.5%㸧ࡢ⾑Ύࡽ HEV 㑇ఏᏊࡀ᳨ฟࡉࢀࡓࡀࠊᕞ࠾ࡼࡧ㛵ᮾᆅ᪉ࡢࣀࢩࢩࡽ ࡣ HEV 㑇ఏᏊࡣ᳨ฟࡉࢀ࡞ࡗࡓ㸦⾲㸱㸧ࠋHEV 㑇ఏᏊࡀ☜ㄆࡉࢀࡓ୰ᅜᆅ᪉ࡢࣀ ࢩࢩ 6 㢌ࢩ࢝ 1 㢌ࡽ᳨ฟࡉࢀࡓ HEV 㑇ఏᏊᆺࢆゎᯒࡋࡓ⤖ᯝࠊࡇࢀࡽࡢ㑇ఏᏊࡣ 㠀ᖖ㏆⦕࡛࠶ࡗࡓࠋ᭦ࠊྠᆅᇦ࡛Ⓨࡋࡓࣄࢺࡢ E ᆺ⫢⅖ᝈ⪅ࡽ᳨ฟࡉࢀࡓ㑇 ఏᏊࡀࡇࡢࢡࣛࢫࢱ࣮ྵࡲࢀࡓࠋࡲࡓࠊࡇࡢᆅᇦࡢ HEV ࡣࠊ᪥ᮏࢆྵࡴࡢᆅ᪉ ࡽ᳨ฟࡉࢀࡓ HEV ࡣ␗࡞ࡿ⊂❧ࡋࡓࢡࣛࢫࢱ࣮ࢆᙧᡂࡋࡓ㸦ᅗ㸯㸧 ࠋ௨ୖࡼࡾࠊ୰ ᅜᆅ᪉࡛ࡣࠊ࠶ࡿ HEV ࡢ㑇ఏᏊᆺࡼࡿࣀࢩࢩࠊࢩ࢝ࠊࣄࢺ㛫࡛ឤᰁ⎔ࡀᡂ❧ࡋ࡚ ࠸ࡓࡇࡀ♧၀ࡉࢀࡓࠋ ⾲ 㸬+(9 ᑐࡍࡿᢠయಖ᭷⋡ ࣀࢩࢩ ࢩ࢝ ୰ᅜᆅ᪉ ᕞᆅ᪉ 㛵ᮾᆅ᪉ ୰ᅜᆅ᪉ ㄪᰝᩘ ᢠయ㝧ᛶᩘ ᢠయ㝧ᛶ⋡㸣 ⾲ 㸬⾑Ύ୰࠾ࡅࡿ +(9 㑇ఏᏊ᳨ฟ⋡ ࣀࢩࢩ ࢩ࢝ ୰ᅜᆅ᪉ ᕞᆅ᪉ 㛵ᮾᆅ᪉ ୰ᅜᆅ᪉ ㄪᰝᩘ 㑇ఏᏊ㝧ᛶᩘ 㑇ఏᏊ㝧ᛶ⋡㸦㸣㸧 ᮏ◊✲ࢆྵࡵࠊ⾑ᾮࡽ E ᆺ⫢⅖࢘ࣝࢫ㸦HEV㸧ࡢ㑇ఏᏊࡀ᳨ฟࡉࢀ࡚࠸ࡿࠋࡍ ࡞ࢃࡕࠊࣀࢩࢩ࠾ࡼࡧࢩ࢝࠾࠸࡚ࡣ㌟ࡢ➽⫗ෆ࠾ࡼࡧྛ⮚ჾ HEV ࡀᏑᅾࡍࡿ ࡇࢆ♧ࡋ࡚࠾ࡾࠊࣀࢩࢩ࠾ࡼࡧࢩ࢝ࢆႚ㣗ࡍࡿሙྜࡣࠊࡢ㒊㛵ࡋ࡚ࡶ༑ศ ຍ⇕ࢆࡍࡿᚲせᛶࡀ࠶ࡿࠋࡲࡓㄪ⌮࡛⏝ࡋࡓჾලࡶ༑ศὙίࡍࡿᚲせࡀ࠶ࡿ㸦ᅗ 㸰㸧 රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ᅗ ࣀࢩࢩ࣭ࢩ࣭࢝ࣄࢺࡽ᳨ฟࡉࢀࡓ +(9 㑇ఏᏊࡢ⣔⤫ゎᯒ ࣀࢩࢩ⏤᮶ᰴࡣ㉥Ꮠ࣭⥳Ꮠࠊࢩ࢝⏤᮶ᰴࡣ㟷Ꮠࠊࣄࢺ⏤᮶ᰴࡣ⣸࡛♧ࡍࠋ ᅗ ( ᆺ⫢⅖࢘ࣝࢫࡢឤᰁࡢྍ⬟ᛶ 㔜⇕ᛶ⾑ᑠᯈῶᑡೃ⩌6)76࢘ࣝࢫ 6)76 ࡢᏛ୍⯡ᛶ≧ 㔜⇕ᛶ⾑ᑠᯈῶᑡೃ⩌㸦SFTS㸧ࡣ SFTS ࢘ࣝࢫ㸦௨ୗࠊSFTSV㸧ࡢឤᰁࡼ ࡾ㸪ࣄࢺⓎ⇕ࠊⓑ⾑⌫ῶᑡࠊ⾑ᑠᯈῶᑡࠊ⫢㓝⣲ࡢୖ᪼࡞ࢆᘬࡁ㉳ࡇࡍࠊ⮴Ṛᛶࡢ 㧗࠸ே⋇ඹ㏻ឤᰁ࡛࠶ࡿࠋ2011 ᖺ୰ᅜ࠾࠸࡚ึࡵ࡚ሗ࿌ࡉࢀࡓẼ࡛࠶ࡾࠊ୰ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ᅜ࡛ࡣࢲࢽࡀᮏ࢘ࣝࢫࢆ፹ࡋࠊࡵࢇ⨺ࠊࣖࢠࠊ࢘ࢩࠊࢾࠊࣈࢱࠊࢽ࣡ࢺࣜ࡞ከ ࡃࡢື≀ࡀ SFTSV ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡿሗ࿌ࡉࢀ࡚࠸ࡿ㸦Yu et al. 2011ࠊᅗ 㸱㸧 ࠋᅜෆ࡛ࡣ 2013 ᖺ 1 ᭶ᒣཱྀ┴࡛ᅜෆึࡢ SFTS ᝈ⪅ࡀሗ࿌ࡉࢀࠊࡑࡢᚋࡢㄪᰝ ࡼࡾࠊ2005 ᖺࡽ SFTS ᝈ⪅ࡀⓎ⏕ࡋ࡚࠸ࡓࡇࡀุ᫂ࡋ࡚࠸ࡿ㸦Takahashi et al. In press㸧ࠋࡑࡢ SFTS ᝈ⪅ࡢⓎ⏕ᮇࡣ 4 ᭶ࡽ 10 ᭶࡛ࢲࢽࡢάືᮇ୍⮴ࡍࡿ ࡇࠊᖺ㱋ู࡛ࡣ 50 ṓ௨ୖࠊᆅᇦู࡛ࡣ୰ᅜࠊᅄᅜࠊᕞᆅ᪉Ⓨ⏕ࡀከ࠸ࡇࡀࠊ ≉ᚩࡋ࡚ሗ࿌ࡉࢀ࡚࠸ࡿ㸦Takahashi et al. In press㸧ࠋ⌧ᅾࠊឤᰁἲᇶ࡙ࡁࠊᝈ ⪅ࢆデ᩿ࡋࡓ་ᖌࡣಖᡤ┤ࡕᒆฟࡿࡇࡀ⩏ົ࡙ࡅࡽࢀ࡚࠸ࡿࡀࠊᅜෆ࠾ࡅ ࡿ SFTS 㛵ࡍࡿሗࡣᑡ࡞࠸ࠋᮏ◊✲࡛ࡣࠊ୰ᅜᆅ᪉ࡢࣀࢩࢩ࠾ࡼࡧࢩ࢝࠾ࡅࡿ SFTSV ࡢឤᰁ≧ἣࢆศᯒࡋࡓࠋ ༸ ⏘༸ Ꮴ 㔝⏕ື≀䞉䝠䝖䞉ᐙ␆ ᡂ䝎䝙 ྾⾑ ྾⾑ 㻿㻲㼀㻿㼂 ⬺⓶ 㻿㻲㼀㻿㼂 ྾⾑ 㻿㻲㼀㻿㼂 ᗂ䝎䝙 ⬺⓶ ⱝ䝎䝙 ᅗ 6)76 ࢘ࣝࢫࡢឤᰁ⎔㔝⏕ື≀ࡢ㛵ಀ ᮦᩱ࠾ࡼࡧ᪉ἲ ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ ୰ᅜᆅ᪉࡛ 2009 ᖺࡽ 2013 ᖺᤕ⋓ࡉࢀࡓࣀࢩࢩ 115 㢌࠾ࡼࡧࢩ࢝ 130 㢌ࡢ⾑ Ύࢆ⏝࠸ࡓࠋ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 6)769 ᑐࡍࡿᢠయࡢ᳨ฟ SFTSV㸦HB29 ᰴ㸧ឤᰁ⣽⬊࠾ࡼࡧ mock ឤᰁ⣽⬊ࢆᢠཎࡋࡓ ELISA ἲࢆ 100ࠊ 400ࠊ1600ࠊ6400 ಸᕼ㔘ࡋࡓࢾ⾑Ύࢆ⏝࠸࡚ᐇࡋࡓࠋḟᢠయࡣ HRP ᶆ㆑ Protein A/G ࢆ⏝࠸ࡓࠋ ⤖ᯝ࠾ࡼࡧ⪃ᐹ ୰ᅜᆅ᪉࡛ᤕ⋓ࡉࢀࡓࣀࢩࢩ 115 㢌୰ 9 㢌㸦8%㸧 ࠊࢩ࢝ 130 㢌୰ 65 㢌㸦50%㸧ࡀ SFTSV ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓ㸦⾲㸲㸧 ࠋࡲࡓࠊ᳃ᕝ㸦2013㸧ࡢ⤖ᯝࡽࠊ 㣫⫱≟ࡼࡾࡶ⊁⊟≟ࡢ᪉ࡀ SFTS ࢘ࣝࢫᢠయࡢ㝧ᛶ⋡ࡀ㧗࠸ࡇࡀሗ࿌ࡉࢀ࡚࠾ࡾࠊ ᒣᯘධࡿᶵࡀከ࠸ឤᰁ⋡ࡀ㧗ࡲࡿྍ⬟ᛶࡀ♧၀ࡉࢀࡓࠋ௨ୖࡼࡾࠊᅜෆึࡢ SFTS ᝈ⪅ࡀ☜ㄆࡉࢀࡓᆅᇦ࿘㎶⏕ᜥࡍࡿࣀࢩࢩ࠾ࡼࡧࢩ࢝࠾࠸࡚ࠊSFTSV ឤ ᰁṔࡀ࠶ࡿࡇࠊ᪥ᮏ࠾࠸࡚ࡶ㔝⏕ື≀ SFTSV ࡢឤᰁࡀᡂ❧ࡍࡿࡇࡀ♧ࡉࢀࡓࠋ ࡇࢀࡲ࡛ࡢሗ࿌ࡽ SFTSV ࡣࢲࢽࡼࡾ፹ࡉࢀࡿࡓࡵࠊᮏ◊✲⤖ᯝࡣࠊࡇࡢᆅᇦ ⏕ᜥࡍࡿࢲࢽࡣ SFTS ࢘ࣝࢫࢆಖ᭷ࡍࡿࡋ࡚࠸ࡿྍ⬟ᛶࡀ࠶ࡿࡇࢆ♧၀ࡋ࡚࠸ࡿࠋ ࡇࡢྍ⬟ᛶࢆ⌮ゎࡋࠊࢲࢽࡼࡿ่ညࢆ㜵ᚚࡍࡿᡭẁࢆྲྀࡿඹࠊSFTS Ⓨࡢ ≧ࡢ≉ᚩࢆࡼࡃ⌮ゎࡋࠊSFTSV ឤᰁࡀࢃࢀࡿሙྜࡣ⮳ᛴ㝔⾜ࡁࠊࢲࢽ่ည ࡉࢀࡓࡇࢆ࿌ࡆ࡚㎿㏿デ᩿ࡋ࡚ࡶࡽ࠺ᚲせࡀ࠶ࡿࠋຍ࠼࡚ࠊࢲࢽࡼࡾ፹ࡉࢀࡿ ឤᰁࡣࠊSFTS ௨እࡶ᪥ᮏ⣚ᩬ⇕ࡸ࣒ࣛࠊࡘࡘࡀࡴࡋ࡞ᵝࠎ࠶ࡿࡓࡵࠊࢲ ࢽࡼࡿ่ညࡣᬑẁࡽ༑ศὀពࡍࡿᚲせࡀ࠶ࡿࠋ ⾲ 㸬6)769 ᑐࡍࡿᢠయಖ᭷⋡ ࣀࢩࢩ ࢩ࢝ ᢠయ㝧ᛶᩘ ᢠయ㝧ᛶ⋡ ㄪᰝᩘ ᮏ◊✲࡛ࣀࢩࢩࡢᢠయ㝧ᛶ⋡ࡀࢩ࢝ẚ࡚పࡗࡓ⤖ᯝ㛵ࡋ࡚ࡣࠊཎᅉࡣ⌧ᅾ ᫂☜ࡣ࡛ࡁ࡞࠸ࡀࠊSFTSV ࢆ፹ࡍࡿࢲࢽࡢ྾⾑ື≀ᑐࡍࡿႴዲᛶࡀ㛵ࡋ࡚࠸ ࡿྍ⬟ᛶࡀ⪃࠼ࡽࢀࡿࡓࡵࠊᚋࡢ◊✲ࡀᮇᚅࡉࢀࡿࠋ ࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫ ࣮࢚࢜ࢫ࣮࢟ࡢᏛ୍⯡ᛶ≧ ࣮࢚࢜ࢫ࣮࢟ࡣ㇜࣊ࣝ࣌ࢫ࢘ࣝࢫ 1㸦௨ୗࠊPRV㸧ࡼࡗ࡚Ⓨࡍࡿࠊࣈࢱ࠾ࡼ ࡧࣀࢩࢩࡀᑐ㇟࡞ࡿᒆฟఏᰁ࡛࠶ࡾࠊᗂ㇜ࡀⓎࡍࡿ⮴Ṛ⋡ࡀ㧗࠸ࡓࡵࠊ㣴㇜ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ᴗከ࡞⤒῭ⓗ⿕ᐖࢆཬࡰࡍࠋឤᰁࡣࠊ㰯Ồࡢ㣕ἓࠊᦤ㣗ࠊയཱྀࡽࡢឤᰁࡸởᰁ≀ ࡢᦤྲྀࡀཎᅉ࡞ࡾࠊᡂ㇜࡛ࡣṚஸ⋡ࡣ㧗ࡃ࡞ࡃࠊከࡃࡢሙྜࡣ㢧ᛶឤᰁ㸨⤊ࢃࡿ ࡇࡀከ࠸ࠋࡋࡋࠊࣈࢱࡸࣀࢩࢩ௨እࡢື≀ឤᰁࡋࡓሙྜࡣࠊ⚄⤒≧࡛࠶ࡿᥙ ࢆᘬࡁ㉳ࡇࡋࠊࡰ 100%Ṛஸࡍࡿࠋឤᰁᚋ PRV ⪏㐣ࡋࡓࣈࢱ࡛ࡣࠊ࢘ࣝ ࢫࡀ₯అឤᰁ㸨㸨ࡍࡿ≉ᚩࡀ࠶ࡾࠊᅜෆ࡛ࡣࠊ ࠕ࣮࢚࢜ࢫ࣮࢟㜵ᑐ⟇せ㡿ࠖᇶ࡙ࡁࠊ ࣈࢱ࠾࠸࡚ࡣ࣡ࢡࢳࣥࢥࣥࢺ࣮ࣟࣝࡼࡾ 36 ┴࡛ PRV ࡢΎίᡂຌࡋ࡚࠾ࡾࠊ 11 ┴࡛Ὑίᑐ⟇ࡀᐇࡉࢀ࡚࠸ࡿ㸦㎰ᯘỈ⏘┬ᾘ㈝࣭Ᏻᒁື≀⾨⏕ㄢ㸧ࠋࡋࡋࠊ ⮫ᗋ≧ࢆ♧ࡋ࡚࠸ࡿࣈࢱࡢⓎ࣭Ὲử௨እࡶࠊ₯అឤᰁࡋ࡚࠸ࡿ㔝እ࢘ࣝࢫᢠయ 㝧ᛶࣈࢱࢆ᪩ᮇⓎぢࡍࡿᚲせࡀ࠶ࡿࠋ ㏆␥ᆅ᪉ࡣࠊ࣮࢚࢜ࢫ࣮࢟ࡢΎίᡂຌࡋ࡚࠸ࡓࡀࠊዉⰋ┴࠾࠸࡚ࠊ 1997 ᖺࣀࢩࢩࡢ⏕⫗ࢆ㣗ࡓ⊟≟ࡀ࣮࢚࢜ࢫ࣮࢟ࡼࡾከᩘṚஸࡋࡓࡇࡀሗ ࿌ࡉࢀ࡚࠸ࡿ㸦ᖾ⏣ 2000㸧 ࠋᚑࡗ࡚ࠊࣀࢩࢩࡣ₯ᅾⓗ࣮࢚࢜ࢫ࣮࢟࢘ࣝ ࢫࡀឤᰁࡋ࡚࠸ࡿྍ⬟ᛶࡀ㧗࠸⪃࠼ࡽࢀࡿࡀࠊᅜෆࡢࣀࢩࢩ࠾ࡅࡿ࣮࢚࢜ࢫ࣮࢟ ࢘ࣝࢫࡢឤᰁ≧ἣࡘ࠸࡚ࡣሗ࿌ࡀ࡞࠸ࠋᮏ◊✲࡛ࡣࠊ࣮࢚࢜ࢫ࣮࢟ࡢ⾑ΎᏛ ㄪᰝࢆᐇࡋࡓࠋ 㸨 㢧ᛶឤᰁ㸸ឤᰁࡣࡋ࡚࠸ࡿࡀࠊⓎࢆకࢃ࡞࠸ឤᰁࠋ 㸨㸨 ₯అឤᰁ㸸࢘ࣝࢫࡣ᳨ฟࡉࢀࡃ࠸ࡀࠊᣢ⥆ⓗឤᰁࡀᡂ❧ࡋ࡚࠸ࡿ≧ἣࠋ ᮦᩱ࠾ࡼࡧ᪉ἲ ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ ࣈࢱ࡛ࡢ࣮࢚࢜ࢫ࣮࢟ࡢΎίᡂຌࡋ࡚࠸ࡿ 3 ┴ࠊ ㏆␥ᆅ᪉ࡢ A ┴࠾ࡼࡧ B ┴ࠊ ୰ᅜᆅ᪉ࡢ C ┴࡛ࠊ2007 ᖺࡽ 2010 ᖺᤕ⋓ࡉࢀࡓࣀࢩࢩࡑࢀࡒࢀ 50 㢌ࠊ71 㢌ࠊ 52 㢌ࡽᚓࡽࢀࡓ⾑Ύࢆ⏝࠸ࡓࠋ 359 ᑐࡍࡿᢠయࡢ᳨ฟ ࢘ࣝࢫࡣ PRV Indiana ᰴࠊ⣽⬊ࡣࣈࢱ⏤᮶ CPK ⣽⬊㸦ື≀⾨⏕◊✲ᡤࡼࡾศ㸧 ࢆ⏝࠸࡚୰ヨ㦂ࡼࡾࠊ⾑Ύ୰ࡢ࢘ࣝࢫᢠయࢆ᳨ฟࡋࡓࠋPRV ᑐࡍࡿᢠయ㝧ᛶ ಶయࢆ᳨ฟࡍࡿࡓࡵࠊ1㸸10 ᕼ㔘⾑Ύࢆ⏝࠸ࡓ 80㸣ࣉ࣮ࣛࢡῶᩘヨ㦂ࢆᐇࡋࡓࠋ 㝧ᛶಶయ㛵ࡋ࡚ࡣࠊ⾑Ύࢆಸ㝵ẁᕼ㔘ࡋ࡚ᢠయ౯ࢆồࡵࡓࠋ ࣡ࢡࢳࣥᰴ㔝እᰴࡢ㆑ู 㔝እᰴឤᰁಶయࡢࡳฟ⌧ࡍࡿ gE ᢠయࡢ᳨ฟࢆࠊIDEXX ♫ࡢ g1 Antibody ELISA ࢆ⏝࠸࡚ᐇࡋࡓࠋ᪉ἲࡣ〇ရῧࡢࣉࣟࢺࢥ࣮ࣝᚑࡗࡓࠋ ⤖ᯝ࠾ࡼࡧ⪃ᐹ ㏆␥ᆅ᪉ࡢ 2 ┴࡛ᤕ⋓ࡉࢀࡓ 6 㢌㸦5%㸧ࡢࣀࢩࢩ࠾࠸࡚ࠊPRV ᑐࡍࡿ࢘ࣝ ࢫ୰ᢠయ㝧ᛶࡀ☜ㄆࡉࢀࡓࠋ୍᪉ࠊ୰ᅜᆅ᪉ࡢ㸯┴࡛ᤕ⋓ࡉࢀࡓࣀࢩࢩ࠾࠸࡚ࡣࠊ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 㝧ᛶಶయࡣ☜ㄆࡉࢀ࡞ࡗࡓ㸦⾲ 5㸧ࠋ࢘ࣝࢫ୰ᢠయಖ᭷࡛࠶ࡗࡓࣀࢩࢩ 6 㢌ࡣࠊ ࡍ࡚ gE ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓࠋᚑࡗ࡚ࣀࢩࢩࡣ࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫࡢ 㔝⏕ᰴឤᰁࡋ࡚࠾ࡾࠊࣈࢱࡢឤᰁ※࡞ࡾᚓࡿࡇࡀࡀ☜ㄆࡉࢀࡓ㸦ᅗ 4㸧ࠋ㎰ሙ ෆ࡛ࡢ PRV Ύίࡀᡂ❧ࡋ࡚࠸ࡿሙྜ࠾࠸࡚ࡶࠊ≉ࣀࢩࢩࡽࡢ㔝እᰴឤᰁࡢ ྍ⬟ᛶࢆ⪃៖ࡋࠊᑐ⟇ࢆᐇࡍࡿࡇࡀᚲせ࡛࠶ࡿࠋ ⾲ 㸬࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫ㸦359㸧ᑐࡍࡿᢠయಖ᭷⋡ ㏆␥ᆅ᪉ ୰ᅜᆅ᪉ $ ┴ % ┴ & ┴ ㄪᰝᩘ ᢠయ㝧ᛶᩘ ᢠయ㝧ᛶ⋡㸣 㣴㇜ 㻟㻢㒔㐨ᗓ┴䛷䝽䜽䝏䞁䝁䞁䝖䝻䞊䝹䛻䜘䜚Ύί 㽢 䞉㣕ἓ 䞉᥋ゐ ⊟≟ 䜲䝜䝅䝅 ⏕⫗䛾ႚ㣗 㔝እᰴ䜴䜲䝹䝇䜢ᣢ䛴ྍ⬟ᛶ䛜㧗䛔 ᅗ ࣮࢚࢜ࢫ࣮࢟࢘ࣝࢫ㸦359㸧ࡢឤᰁࡢྍ⬟ᛶ ᪥ᮏ⬻⅖ ᪥ᮏ⬻⅖ࡢᏛ୍⯡ᛶ≧ ᪥ᮏ⬻⅖ࡣࠊ᪥ᮏ⬻⅖࢘ࣝࢫ㸦௨ୗࠊJEV㸧ࡀཎᅉࡢឤᰁ࡛ࠊ့ங㢮ࡽ㫽㢮ࡲ ࡛ᗈ⠊࡞ື≀✀ឤᰁࡍࡿࡀࠊ㒊ศࡣ㢧ᛶឤᰁࢆ♧ࡍࠋࣄࢺࡽࣄࢺࡢឤᰁࡣ࡞ ࡃࠊቑᖜື≀㸨㸦ࣈࢱ㸧ࡢయෆ࡛࠸ࡗࡓࢇቑ࠼࡚⾑ᾮ୰ฟ࡚ࡁࡓ࢘ࣝࢫࢆࠊ⺅ࡀ྾ ⾑ࡋࠊࡑࡢୖ࡛ࣄࢺ࡞ࢆ่ࡋࡓឤᰁࡍࡿࠋࣄࢺࡸ㤿ࡣឤᰁࡍࡿ㔜⠜࡞⬻⅖ࢆⓎ ࡍࡿࡇࡀ࠶ࡿࡀࠊᅜෆ࡛ࡣ㏆ᖺ࣡ࢡࢳࣥ᥋✀ࡼࡾࠊࣄࢺࡸ㤿࡛ࡢⓎ⏕ࡣ⃭ῶࡋ࡚ ࠸ࡿࠋࣈࢱࡣዷፎ㇜࡛ὶ⏘ࢆᘬࡁ㉳ࡇࡍࡇࡀ࠶ࡾࠊࡲࡓ㏆ᖺࠊ࢘ࢩ࡛ࡢ JEV ࡼࡿ ⬻⅖ࡢⓎ⏕ࡀᐑᓮ┴ࡸឡ▱┴࡛ሗ࿌ࡉࢀ࡚࠸ࡿ㸦ຍྂ 2011; ∦ᒣ 2013㸧ࠋ᪥ᮏ⬻ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ⅖ࡣࠊឤᰁἲᇶ࡙ࡁࠊᝈ⪅ࢆデ᩿ࡋࡓ་ᖌࡣಖᡤ┤ࡕᒆฟࡿࡇࡀ⩏ົ࡙ࡅ ࡽࢀ࡚࠸ࡿࠋ 2008 ᖺ 12 ᭶ࠊරᗜ┴ෆ࡛ᤕ⋓ࡉࢀࡓࣀࢩࢩࡢ⾑ᾮ୰ࡽ࢘ࣝࢫࡀศ㞳ࡉࢀࠊ ᭦ 2009 ᖺ 5 ᭶රᗜ┴࠾࠸࡚ᤕ⋓ࡉࢀࡓࣀࢩࢩࡢ⾑ᾮࡽࡣ࢘ࣝࢫ㑇ఏᏊࡀ ᳨ฟࡉࢀࡓ㸦㧗ᓮ 2009㸧ࠋࡑࡢࡓࡵࠊ᪥ᮏ⬻⅖࢘ࣝࢫࡢឤᰁ⎔࠾࠸࡚ࠊࣀࢩ ࢩࡶቑᖜື≀࡞ࡿྍ⬟ᛶࡀᣦࡉࢀ࡚࠸ࡿࠋᮏ◊✲࡛ࡣࠊ㏆␥ᆅ᪉࠾ࡼࡧ୰ᅜᆅ᪉ࡢ ࣀࢩࢩ࠾ࡼࡧࢩ࢝࠾ࡅࡿ JEV ࡢឤᰁ≧ἣࢆศᯒࡋࡓࠋ 㸨 ቑᖜື≀㸸࢘ࣝࢫࢆయෆ࡛ቑᖜࡋࠊࡢᐟ౪⤥ࡍࡿࠋ ᮦᩱ࠾ࡼࡧ᪉ἲ ᳨ᰝᑐ㇟ᆅᇦ࠾ࡼࡧࢧࣥࣉࣝᩘ ㏆␥ᆅ᪉࡛ 2009 ᖺࡽ 2013 ᖺᤕ⋓ࡉࢀࡓࣀࢩࢩ 33 㢌ࠊࢩ࢝ 25 㢌ࠊ࠾ࡼࡧ୰ ᅜᆅ᪉࡛ᤕ⋓ࡉࢀࡓࣀࢩࢩ 63 㢌ࡼࡾᚓࡓ⾑Ύࢆ⏝࠸ࡓࠋ -(9 ᑐࡍࡿᢠయࡢ᳨ฟ JEV/sw/Chiba/88/2002 ᰴࢆ⏝࠸࡚ 80㸣ࣉ࣮ࣛࢡῶᩘヨ㦂ࡼࡾ୰ᢠయ౯ࢆ ᐃ ࡋࡓࠋ10 ಸ௨ୖࡢᢠయ౯ࢆᬻᐃⓗ㝧ᛶุᐃࡋࡓࠋ ⤖ᯝ࠾ࡼࡧ⪃ᐹ ㏆␥ᆅ᪉ࡢࣀࢩࢩ 33 㢌୰ 22 㢌㸦67%㸧ࠊࢩ࢝ 25 㢌୰ 23 㢌㸦92%㸧 ࠊ୰ᅜᆅ᪉ࡢ ࣀࢩࢩ 63 㢌୰ 62 㢌㸦98%㸧ࡀࠊJEV ᑐࡍࡿᢠయࢆಖ᭷ࡋ࡚࠸ࡓ㸦⾲㸴㸧ࠋࣀࢩ ࢩࡢ᪥ᮏ⬻⅖ᑐࡍࡿᢠయಖ᭷⋡ࡣ㠀ᖖ㧗ࡃࠊࣈࢱྠᵝቑᖜື≀࡞ࡗ࡚࠸ࡿྍ ⬟ᛶࡀ♧၀ࡉࢀࡓࠋࡲࡓࠊࢩ࢝ࡘ࠸࡚ࡶᢠయ㝧ᛶ⋡ࡀ㠀ᖖ㧗ࡗࡓࡓࡵࠊ᪂ࡓ࡞ឤ ᰁ※ࡋ࡚ὀពࢆࡍࡿᚲせࡀ࠶ࡿ㸦ᅗ㸳㸧 ࠋ ⾲ 㸬᪥ᮏ⬻⅖࢘ࣝࢫ㸦-(9㸧ᑐࡍࡿᢠయಖ᭷⋡ ㏆␥ᆅ᪉ ୰ᅜᆅ᪉ ࣀࢩࢩ ࢩ࢝ ࣀࢩࢩ ㄪᰝᩘ ᢠయ㝧ᛶᩘ ᢠయ㝧ᛶ⋡ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ឤᰁ䛩䜛ື≀ ྾⾑ ቑᖜື≀䛸⪃䛘䜙䜜䜛㔝⏕ື≀䞉ᐙ␆ ྾⾑ ᅗ ᪥ᮏ⬻⅖࢘ࣝࢫ㸦-(9㸧ࡢឤᰁ⎔࠾ࡅࡿ㔝⏕ື≀ࡢᙺ ࡲࡵ ᡃࠎࡣࠊ㔝⏕ື≀࡛ࡢ⾑ΎᏛㄪᰝࢆᐜ᫆ࡘ≉␗ⓗᐇࡍࡿࡇࢆ┠ⓗࡋ࡚ࠊ ᇵ㣴⣽⬊࡚Ⓨ⌧ࡋࡓࢱࣥࣃࢡ㉁ࢆᢠཎࡋ࡚ࠊProteinA/G ࢆ⏝࠸ࡓ ELISA ⣔ࡢᵓ⠏ ᡂຌࡋࡓࠋࡉࡽᡃࠎࡢᏛㄪᰝࡼࡾࠊE ᆺ⫢⅖ࠊSFTSࠊ࣮࢚࢜ࢫ࣮࢟ࠊ᪥ᮏ ⬻⅖ࡢཎᅉ࢘ࣝࢫࡣࠊࣀࢩࢩࡀಖ᭷ࡋ࡚࠸ࡿྍ⬟ᛶࡀ㧗ࡃࠊࣄࢺ࠾ࡼࡧࣈࢱࢆྵࡴ ᵝࠎ࡞ື≀ᑐࡋ࡚ࠊࣀࢩࢩࡀࡇࢀࡽࡢឤᰁࡢឤᰁ※࡞ࡿࡇࡀ♧၀ࡉࢀࡓࠋࡲ ࡓࠊࡇࢀࡽࡢឤᰁࡢឤᰁ⤒㊰ࡣࠊᦤ㣗ࠊ྾⾑᪻ࡼࡿ፹ࠊ㣕ἓ࡞ᵝࠎ࡛࠶ࡿࠋ ࡇࢀࡽࡢ⤖ᯝࡽࠊ㔝⏕ື≀᥋ゐࡍࡿᶵࡀከ࠸ࠊ⊁⊟ࢆࡍࡿ᪉ࠎࠊ㔝⏕ື≀ࢆ㣗⫗ ࡋ࡚⏝ࡉࢀࡿ᪉ࠎࠊ㣴㇜ᴗ⪅ࡢ᪉ࠎᑐࡋࠊᅇㄪᰝࡋࡓឤᰁࡢឤᰁࢆண㜵ࡍࡿ ᪉ἲࡋ࡚ࠊ௨ୗࡢὀព㡯ࢆᥦゝࡍࡿࠋ 㔝⏕ື≀⟶⌮㛵ࢃࡿ᪉ཬࡧࣀࢩࢩࡀ⏕ᜥࡍࡿᆅᇦ࠾ఫ࠸ࡢ᪉ ࡢ࡞࢝ࢩࡸࢩࢩࣀۑ㔝⏕ື≀ࢆゎయࡍࡿ㝿ࡣࠊᡭ⿄ࢆ╔⏝ࡋࠊ⾑ᾮࡼࡿ ởᰁࢆ㜵ࡄ ࡓࡋ⏝ۑໟࡸࡲ࡞ᯈ࡞ࡢ㐨ල㢮ࡶࠊᚲࡎᑀὙίࡍࡿ ⏕ࠊࡵྵࡶ≟⊟ۑ㣗ࡣཝ⚗࡛࠶ࡿࠋႚ㣗ࡣᚲࡎ༑ศຍ⇕ࡍࡿ ۑ㔝ᒣ⾜ࡃ㝿ࡣࠊ⺅ࡸࢲࢽ࡞ࡢ྾⾑᪻ࡼࡿ྾⾑ࢆண㜵ࡍࡿࡓࡵࠊ㛗 ⿇㺃㛗ࢬ࣎ࣥࡢ╔⏝ࠊ㝖ࡅ࡞ࢆᐇࡍࡿ ࢱࣈࡣࢩࢩࣀۑඹ㏻ឤᰁࡍࡿཎయࢆಖ᭷ࡋ࡚࠸ࡿࡓࡵࠊ㣫⫱ሙෆࡢ ࣀࢩࢩࡢධࢆ㜵Ṇࡍࡿ රᗜ࣡ࣝࢻࣛࣇࣔࣀࢢࣛࣇ 6-8 ࡲࡓࠊᅇㄪᰝࡋࡓ࢘ࣝࢫឤᰁ௨እࡶࠊࣀࢩࢩ➼㔝⏕ື≀ࡀឤᰁ※࡞ࡿឤ ᰁࡀᏑᅾࡍࡿࡇࡸࠊᮍࡔⓎぢࡉࢀ࡞࠸࢘ࣝࢫࢆ㔝⏕ື≀ࡀಖ᭷ࡋ࡚࠸ࡿྍ⬟ᛶࡀ ࠶ࡿࡇࡽࡶࠊୖグࡢ㡯ࡣࠊᖖẼࢆࡅ࡚㡬ࡁࡓ࠸ࠋ ᘬ⏝ᩥ⊩ Hara, Y., Terada, Y., Yonemitsu, K., Shimoda, H., Noguchi, K., Suzuki, K. and Maeda, K. 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