Comments
Description
Transcript
9 - BRIC
アプリケーションデータ集03 01.11.12 0:16 PM ページ 75 医薬品、生体関連物質 9 Drugs, Metabolites 市販鎮痛解熱剤 Pain-killing drug インドメタシン Indometacin 1.Acetaminophen 2.Caffeine 3.Ethenzamide 4.Bromovalerylurea 1.p-Chlorobenzoic acid 2.p-Hydroxybenzoic acid n-butyl ester 3.Indometacin 2 1 3 (G900727F) (G900727D) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/0.1% phosphoric acid (70/30) Flow rate : 1.3 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.64 AUFS Injection : 20 μL Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/0.1% phosphoric acid (3/17) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (日本薬局方第12改正記載条件) 市販風邪薬成分 A cough/cold medication 風邪薬成分 Ingredients in a cough/cold medication 1.Caffeine 2.Chlorpheniramine maleate 3.Narcotine 4.Ethenzamide 5.Bucetine 1.Acetaminophen 2.Caffeine 3.Chloropheniramine malate 4.Narcotine 5.Ethenzamide 6.Bucetine (G910523U) (G910523E) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 3 μL (10 mg/mL) Sample : an extract from a commercially available cough/cold preparation Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 10 μL (0.02 ∼0.22 mg/mL) 75 9 アプリケーションデータ集03 01.11.12 0:16 PM ページ 76 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites 風邪薬成分 Ingredients in a cough/cold medication 風邪薬成分 Ingredients in a cough/cold medication 1.Acetaminophen 2.Caffeine 3.Chloropheniramine malate 4.Narcotine 5.Ethenzamide 6.Bucetine 解熱鎮痛薬 Pain-killing drugs コデイン Codeine 1.Acetaminophen 2.Caffeine 3.Chloropheniramine malate 4.Narcotine 5.Ethenzamide 6.Bucetine Codeine (G910523F) (G910523G) (A001019B) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 17 μL (0.02∼0.22 mg/mL) : YMC-Pack ODS-A (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 17 μL (0.02∼0.22 mg/mL) : YMC-Pack Pro C4, Pro C8 and Pro C18 (5 μm,12nm) 150 × 4.6 mm I.D. Eluent : methanol/20mM H 3PO4 (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 10 μL (0.01 - 2.0 mg/mL) 解熱鎮痛薬成分 Analgesics 解熱鎮痛薬成分 Analgesics 鼻炎用点鼻剤 Collunarium Column 9 Column Column (J010528A) Column Eluent Flow rate Detection Injection 塩酸ナファゾリンとマレイン酸クロルフェニラミン Naphazoline hydrochloride and chlorpheniramine maleate 1.Naphazoline hydrochloride 2.Chlorpheniramine maleate 1 1 1 医 薬 品 ・ 生 体 関 連 物 質 : YMC-Pack Pro C18 (5μm,12nm) 150 × 4.6 mm I.D. : acetonitrile/0.05% TFA (10/90) : 1.5 mL/min : UV at 210 nm : 5 μL (0.1 mg/mL) Acetaminophen Acetaminophen 2 Naphazoline hydrochloride 2 2 Caffeine 3 Caffeine 3 Chlorpheniramine maleate Ethenzamide 3 Ethenzamide 4 4 Apronalide Benzethonium chloride Apronalide Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (S930422D) (S930422G) (Y920610A) Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (40/60) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.51 AUFS Injection : 5 μL (0.2∼2.0 mg/mL) 76 Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (40/60) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.51 AUFS Injection : 5 μL (0.2 ∼2.0 mg/mL) : YMC-Pack Ph (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/NH4SCN (48/52/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 2.56 AUFS Injection : 10 μL (0.2 ∼5 mg/mL) Sample : collunarium (T920526A) Column Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water/NH4 SCN (35/65/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 10 μL (0.125 mg/mL) 77 9 アプリケーションデータ集03 01.11.12 0:16 PM ページ 78 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites 塩化ベンゼトニウム Benzethonium chloride 塩化ベンザルコニウム Benzalkonium chloride 抗ヒスタミン薬 Histamine antagonists 抗ヒスタミン薬 Histamine antagonists 1.Benzethonium chloride 1.Benzalkonium chloride R=-C12H25 2.Benzalkonium chloride R=-C14H29 Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (T920527A) Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (J010619A) (T920529D) (A980723A) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.16 AUFS Injection : 10 μL (1.0 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.02 AUFS Injection : 10 μL (1.0 mg/mL benzalkonium chloride) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 5 μL (0.05 - 2.0 mg/mL) : YMC-Pack Pro C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : acetonitrile / 50mM CH3COONH4 (40/60) Flow rate : 0.2 mL/min Temperature : 37℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 2 μL (0.05 - 2.0 mg/mL) 鼻炎薬成分 Ingredients in a drug for rhinitis 点眼剤成分 Ingredients in an eye drop 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 9 Column 医 薬 品 ・ 生 体 関 連 物 質 9 1 1 Piroxicam Piroxicam 2 2 Sulindac Sulindac 3 3 Tolmetin sodium dihydrate Tolmetin sodium dihydrate 4 4 Ketoprofen Ketoprofen 5 5 (J010418B) Column : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5) B)acetonitrile 0-40%B(0-10min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.064 AUFS Injection : 3 μL (0.02 - 0.5 mg/mL) 78 : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : A)20mM KH 2PO4 - H3PO4 (pH 2.5) B)acetonitrile 0-60%B(0-10min), 60%B(10-15min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 265 nm, 0.128 AUFS Injection : 5 μL (0.03 - 0.3 mg/mL) Naproxen Naproxen (J010406A) (O950126A) Column Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 1-propanol/water/acetic acid (30/70/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.04 AUFS Injection : 3 μL (20 μg/mL) (O950124A) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 2-propanol/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.04 AUFS Injection : 5 μL (20 μg/mL) 79 アプリケーションデータ集03 01.11.12 0:16 PM ページ 80 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 三環系抗うつ薬 Tricyclic antidepressants 1 1 三環系抗うつ薬 Tricyclic antidepressants 1 Ketoprofen Fenoprofen calcium dihydrate Desipramine hydrochloride 2 2 2 Flurbiprofen Fenoprofen calcium dihydrate Acenaphthene (I.S.) 3 3 3 Ibuprofen Flurbiprofen Imipramine hydrochloride 4 4 Ibuprofen Amitriptyline hydrochloride (O950125C) (A970321A) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (65/35/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.04 AUFS Injection : 7 μL (160, 16, 820 μg/mL) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (65/35/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.032 AUFS Injection : 7 μL (6∼820 μg/mL) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (0.1∼0.5 mg/mL) 三環系抗うつ薬 Tricyclic antidepressants 三環系抗うつ薬 Tricyclic antidepressants 抗精神病薬 Antipsychotic drugs Column 1 2 9 (A980827E) (A961016B) Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol / 20mM CH3 COONH4 (75/25) Flow rate : 1.0 mL/min Temperature : 37℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 5 μL (0.1-0.5 mg/mL) 抗てんかん薬 Antiepileptics Perphenazine 9 1 1 1 医 薬 品 ・ 生 体 関 連 物 質 Prochlorperazine maleate 3 Ethosuximide Doxepin hydrochloride 2 Desipramine hydrochloride Promazine hydrochloride 4 2 2 Primidone Fluphenazine hydrochloride 5 Desipramine hydrochloride Acenaphthene 3 (I.S.) 3 Trifluoperazine hydrochloride 3 6 Phenobarbital sodium salt 4 Ethopropazine hydrochloride Imipramine hydrochloride 7 (Profenamine hydrochloride) Imipramine hydrochloride 4 Phenytoin 4 8 5 Chlorpromazine hydrochloride Amitriptyline hydrochloride Amitriptyline hydrochloride Carbamazepine Thioridazine hydrochloride (D960724A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 215 nm, 0.13 AUFS Injection : 2 μL (0.125 mg/mL) 80 (A961015G) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (0.1∼0.5 mg/mL) (O950627B) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (30/70/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.064 AUFS Injection : 5 μL (0.02 mg/mL) (S960723A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4 H2 PO4 - (NH4 )2 HPO4 (pH 6.7)/methanol (50/50) Flow rate : 0.7 mL/min Temperature : 37 ℃ Detection : UV at 215 nm, 0.32 AUFS Injection : 8 μL (0.035 ∼0.7 mg/mL) 81 アプリケーションデータ集03 01.11.12 0:16 PM ページ 82 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites 抗高脂血症剤 Hypolipidemic drugs 抗高脂血症剤 Hypolipidemic drugs カルシウム拮抗薬 Calcium antagonists カルシウム拮抗薬 Calcium antagonists 5 1 5 1 1 1 Prenylamine lactate Prenylamine lactate Diltiazem HCl Diltiazem HCl Bezafibrate Bezafibrate 2 6 Bepridil HCl 2 2 Bepridil HCl Verapamil HCl Verapamil HCl 7 3 7 3 Gemfibrozil Gemfibrozil 6 2 Methoxyverapamil HCl Methoxyverapamil HCl 3 3 Niguldipine HCl Niguldipine HCl 4 4 8 8 Clofibrate Clofibrate Nicardipine HCl Nicardipine HCl 4 4 Nifedipine Nifedipine Fenofibrate Fenofibrate (S930702C) (S930706E) (S930701D) (S930706C) Column Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid(60/40/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 275 nm, 0.064 AUFS Injection : 8 μL (0.05∼0.25 mg/mL) : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid(60/40/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 275 nm, 0.064 AUFS Injection : 8 μL (0.05∼0.25 mg/mL) : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2 PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA・Br (75/15/10/0.5, v/v/v/w) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.064 AUFS Injection : 8 μL (0.05 ∼1.4 mg/mL) : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2 PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA・Br (70/20/10/0.35, v/v/v/w) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.064 AUFS Injection : 8 μL (0.05 ∼1.4 mg/mL) 抗高脂血症剤 Hypolipidemic drugs ロバスタチン Lovastatin カルシウム拮抗薬 Calcium antagonists 不整脈治療薬 Antiarrhythmic drugs Column 1 9 Column 5 9 1 Prenylamine lactate 医 薬 品 ・ 生 体 関 連 物 質 Bezafibrate Diltiazem HCl 2 6 2 Bepridil HCl Verapamil HCl Gemfibrozil 7 3 Lovastatin 3 Methoxyverapamil HCl 4 Niguldipine HCl 8 Clofibrate Nicardipine HCl 4 Nifedipine Fenofibrate (S930702A) (S930706B) (J010528B) (S990819B) : J'sphere ODS-L80 (4 μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2 PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA・Br (70/20/10/0.35, v/v/v/w) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.064 AUFS Injection : 8 μL (0.05∼1.4 mg/mL) Column Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid(60/40/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 275 nm, 0.064 AUFS Injection : 8 μL (0.05∼0.25 mg/mL) 82 Column Eluent Flow rate Detection Injection : YMC-Pack Pro C18 (5 μm,12nm) 150 × 4.6 mm I.D. : acetonitrile/0.05% formic acid (30/70) : 1.5 mL/min : UV at 240 nm : 5 μL (0.1 mg/mL) Column : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 5 μL (0.05 - 0.1 mg/mL) 83 アプリケーションデータ集03 01.11.12 0:16 PM ページ 84 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites 不整脈治療薬 Antiarrhythmic drugs 局所麻酔薬 Local anesthetics 利尿薬 Diuretics 制がん剤および関連化合物 Antineoplastic and related compounds 1 Uracil 2 5-Fluorouracil 3 Deoxyuridine 4 5-Fluoro-2'-deoxyuridine (S990817C) (J010302E) (A980716A) (T920924E) Column : YMC-Pack Pro C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2 PO4 - K2HPO4 (pH 6.9) / methanol (40/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 5 μL (0.05 - 0.1 mg/mL) Column : Hydrosphere C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (40/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.02 - 0.5 mg/mL) Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : methanol / 1% acetic acid (40/60) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.08 AUFS Injection : 3 μL (0.015 - 0.2 mg/mL) : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 10mM NH4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.025∼0.2 mg/mL) 利尿薬 Diuretics 利尿薬 Diuretics 制がん剤および関連化合物 Antineoplastic and related compounds 葉酸及び葉酸拮抗物質 Folic acid and its antagonists 9 1 1 医 薬 品 ・ 生 体 関 連 物 質 Column 9 1 1 Uracil Aminopterin 2 Hydrochlorothiazide 2 Hydrochlorothiazide 2 2 5-Fluorouracil Methotrexate 3 3 Chlorthalidone Chlorthalidone Folic acid 3 Deoxyuridine 3 4 Furosemide Furosemide 5-Fluoro-2'-deoxyuridine (A970425C) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH 4H2PO4 - H3PO4(pH 3.2)/methanol (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.16 AUFS Injection : 15 μL (0.015∼0.2mg/mL) 84 (A970426D) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4 H2 PO4 - H3PO4(pH 3.2)/methanol (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.16 AUFS Injection : 15 μL (0.015∼0.2mg/mL) (T920924G) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 10mM NH 4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.025∼0.2 mg/mL) (O950411B) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Temperature : ambient(25 ℃) Detection : UV at 300 nm, 0.04 AUFS Injection : 5 μL (16 μg/mL) 85 アプリケーションデータ集03 01.11.12 0:16 PM ページ 86 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites 抗HIVヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents 抗HIVヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents ニコチン酸構造類縁体 Nicotinic acid analogues 塩基性化合物 Basic compounds 1 1 Uracil 1 Isonicotinic acid 1 2 2 Procainamide DDC DDC 3 Nicotinic acid 2 2 N-Acetylprocainamide 3 DDI 4 DDI Isonicotinic acid hydrazide 4'-Hydroxyacetophenone 3 4 3 D4T Nicotinamide D4T 4 5 4 N' -Methylnicotinamide AZT AZT (K930311C) (K930311A) (K940125K) (T931217C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 7 μL (0.125 mg/mL) Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 7 μL (0.125 mg/mL) Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.64 AUFS Injection : 13 μL (0.2 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM K2HPO4 - KH2PO4 (pH 6.9)/acetonitrile (92/8) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 10 μL (30∼300 μg/mL) 抗HIVヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents ニコチン酸構造類縁体 Nicotinic acid analogues 塩基性化合物 Basic compounds 殺菌薬 Disinfectants 1 9 医 薬 品 ・ 生 体 関 連 物 質 Benzoic acid Uracil 2 Isonicotinic acid 1 9 1 1 2 2 Procainamide Salicylic acid 3 DDC Nicotinic acid 3 2 3 N-Acetylprocainamide 4 Theophylline (I.S.) DDI Isonicotinic acid hydrazide 4 4'-Hydroxyacetophenone 4 Phenol 3 Nicotinamide D4T 5 4 N' -Methylnicotinamide AZT (S930617D) (K930311F) (K940125I) (T931217B) Column Column : YMC-Pack C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 7 μL (0.125 mg/mL) 86 Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.64 AUFS Injection : 13 μL (0.2 mg/mL) Column : YMC-Pack PolymerC18 150 × 4.6 mm I.D. Eluent : 20mM K2 HPO4 - KH2PO4 (pH 6.9)/acetonitrile (92/8) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 10 μL (30∼300 μg/mL) : YMC-Pack ODS-AL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM KH2PO4 - Na2HPO4 (pH 7.0)/methanol (75/25) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.13 AUFS Injection : 3 μL (0.1∼0.8 mg/mL) 日本薬局方第12改正記載条件 87 アプリケーションデータ集03 01.11.12 0:16 PM ページ 88 医薬品、生体関連物質 医薬品、生体関連物質 9 Drugs, Metabolites 9 Drugs, Metabolites アシクロビル Acyclovir 塩化カルニチン Carnitine chloride プリン代謝物 Purine catabolites プリン代謝物 Purine catabolites 1.Allantoin 2.Allantoic acid 3.Uric acid 1.Allantoin 2.Allantoic acid 3.Uric acid Acyclovir (F000629A) (J010528C) Column Eluent Flow rate Detection Injection : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NaH2PO4-H3PO4 (pH2.6) containing 15mM 1-pentanesulfonic acid sodium salt Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 4 μL (0.2 ∼20 mg/mL) (G910607G) (G910618A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. : acetonitrile / 0.05% formic acid (3 / 97) : 1.5 mL/min : UV at 254 nm : 5μL (0.1 mg/mL) キサンチンオキシダーゼ阻害剤および関連化合物 Xanthine oxidase inhibitor and related compounds 9 キサンチンオキシダーゼ阻害剤および関連化合物 Xanthine oxidase inhibitor and related compounds Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : 10mM CH 3COOH Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 200 nm, 0.16 AUFS Injection : 4 μL (0.10 ∼0.71 mg/mL) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : 10mM CH3COOH Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 200 nm, 0.16 AUFS Injection : 4 μL (0.10 ∼0.71 mg/mL) カテコールアミン Catecholamines 馬尿酸類 Hippuric and methylhippuric acids 1 医 薬 品 ・ 生 体 関 連 物 質 9 1 1 1. Norepinephrine hydrochloride 2. Epinephrine 3. 3,4-Dihydroxyphenylalanine (DOPA) 4. Dopamine hydrochloride 5. 4-Hydroxy-3-methoxymandelic acid 6. 3-Methoxytyramine hydrochloride 7. 4-Hydroxy-3-methoxyphenylglycol 8. 3,4-Dihydroxyphenylacetic acid 9. Homovanilic acid Uric acid Uric acid 2 2 Hypoxanthine Hypoxanthine 3 Mandelic acid 2 Hippuric acid 3 3 Xanthine o-Methylhippuric acid Xanthine 4 4 4 Oxipurinol m-Methylhippuric acid Oxipurinol 5 5. 5 Allopurinol Allopurinol p-Methylhippuric acid (F000817A) (S930728J) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NH 4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.51 AUFS Injection : 8 μL (0.05∼0.2 mg/mL) 88 (S930729B) : J'sphere ODS-L80 (4 μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.51 AUFS Injection : 8 μL (0.05 ∼0.2 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3 PO4(pH 3.1) B)50mM KH2PO4-H3 PO4(pH 3.1)/acetonitrile (90/10) 0%B (0-5min), 0-100%B (5-25min,linear), 100%B (25-35min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.64 AUFS Injection : 10 μL (0.15 - 0.25 mg/mL) (A961114A) Column Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 225 nm, 0.16 AUFS Injection : 10 μL (0.04 ∼0.07 mg/mL) 89 アプリケーションデータ集03 01.11.12 0:16 PM ページ 90 医薬品、生体関連物質 抗菌剤、抗生物質 9 Drugs, Metabolites 10 Antibiotics 馬尿酸類 Hippuric and methylhippuric acids 合成抗菌剤 Antibacterial agents 合成抗菌剤 Antibacterial agents 1. Olaquindox 2. Carbadox 3. Thiamphenicol 4. Sulfamerazine 5. Sulfadimidine 6. Sulfamonomethoxine 7. Furazolidone 8. Oxolinic acid 9. Sulfaquinoxaline 10. Sulfadimethoxine 11. Nalidixic acid 12. Piromidic acid 1 Mandelic acid 2 Hippuric acid 1. Olaquindox 2. Carbadox 3. Thiamphenicol 4. Sulfamerazine 5. Sulfadimidine 6. Sulfamonomethoxine 7. Furazolidone 8. Oxolinic acid 9. Sulfaquinoxaline 10. Sulfadimethoxine 11. Nalidixic acid 12. Piromidic acid 3 o-Methylhippuric acid 4 m-Methylhippuric acid 5 p-Methylhippuric acid (K940614B) (A961119A) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2 PO4 - H3PO4 (pH 3.2)/methanol (80/20) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 225 nm, 0.16 AUFS Injection : 10 μL (0.04∼0.07 mg/mL) (K940615C) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) B)acetonitrile/50mM NH4 H2 PO4 (80/20) 25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.32 AUFS Injection : 10 μL (0.05 mg/mL) : J'sphere ODS-L80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4 H2 PO4 (10/90) B)acetonitrile/50mM NH4H2PO4 (80/20) 25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.32 AUFS Injection : 10 μL (0.05 mg/mL) 合成抗菌剤 Antibacterial agents サルファ剤 Sulfonamides 9 Column 1 1 6 Sulfaguanidine 医 薬 品 ・ 生 体 関 連 物 質 2 7 Sulfapyridine Diaveridine Sulfanilamide 2 3 Trimethoprim 4 8 Sulfisomidine 9 Sulfamerazine 10 Sulfamethizole 3 Sulfathiazole 5 Pyrimethamine 10 Sulfadiazine (O950320G) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 5 μL (20 μg/mL) 90 Sulfamethoxypyridazine Sulfamethazine (O950306E) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (10/90) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 3 μL (50 μg/mL) 91 アプリケーションデータ集03 01.11.12 0:16 PM ページ 92 抗菌剤、抗生物質 抗菌剤、抗生物質 10 Antibiotics 10 Antibiotics サルファ剤 Sulfonamides サルファ剤 Sulfonamides 1 サルファ剤 Sulfonamides 1 6 抗生物質 Antibiotics 1.Ampicilline 2.Cephalexin 3.Oxytetracycline hydrochloride 4.Tetracycline 5.Chlorotetracycline 6.Chloramphenichol 7.Oxolinic acid 8.Doxorubicin hydrochloride 9.Nalidixic acid Sulfamethazine 2 Sulfaguanidine 2 Sulfathiazole 7 Sulfadoxine 3 Sulfamethizole 3 Trimethoprim 8 Sulfamonomethoxine hydrate 4 4 Sulfapyridine Sulfanilamide 9 Sulfamerazine Sulfisomidine 5 5 10 Sulfamethoxypyridazine Sulfadiazine 6 11 Sulfisoxazole Sulfamethoxazole Sulfachloropyridazine Sulfamethazine 7 8 Sulfadimethoxine (G910523M) Sulfaquinoxaline sodium (J010130D) (O950324L) (O950302C) Column Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO 4 (pH 6.2)/THF (90/10) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 10 μL (18 μg/mL) : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : THF/water/acetic acid (30/70/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 5 μL (42 μg/mL) : Hydrosphere C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : 10mM CH 3COOH - CH3COONH4 (pH 4.6) / acetonitrile (85/15) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 2 μL (0.02 mg/mL) サルファ剤 Sulfonamides サルファ剤 Sulfonamides ナリジクス酸系抗生物質 Nalidixic acid antibiotics Column 1 6 1 Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) B)acetonitrile/50mM NH4H2PO4 (50/50) 0-55%B (0-30min,linear), 55%B (30-40min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.16 AUFS Injection : 15 μL (0.01 ∼0.15 mg/mL) ナリジクス酸系抗生物質 Nalidixic acid antibiotics Pipemidic acid Pipemidic acid 2 Sulfisomidine 2 Sulfamonomethoxine hydrate 7 2 Enoxacin Enoxacin 3 Sulfathiazole 10 3 Sulfadoxine 8 3 Cinoxacin 抗 菌 剤 ・ 抗 生 物 質 4 Sulfamethoxazole 9 4 Norfloxacin Norfloxacin 5 Sulfamerazine 5 5 Sulfadimethoxine 10 Ofloxacin Ofloxacin 6 Sulfamethazine 10 Cinoxacin 4 Sulfadiazine 1 6 Sulfaquinoxaline sodium Nalidixic acid Nalidixic acid 7 7 Oxolinic acid Oxolinic acid 8 8 Piromidic acid Piromidic acid (O950428A) (J010321F) (O950428D) (O950217D) Column Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/acetonitrile/50mM NH4H2PO4 (15/5/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 3 μL (50 μg/mL) 92 : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : 10mM CH 3COOH - CH3COONH4 (pH 4.6) / acetonitrile (85/15) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 10 μL (0.01 - 0.04 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10) containing 20mM TBA・Br Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 5 μL (0.03 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4 H2 PO4 - (NH4)2 HPO4 (pH 6.7)/tetrahydrofuran (90/10) containing 20mM TBA・Br Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 5 μL (0.03 mg/mL) 93 アプリケーションデータ集03 01.11.12 0:16 PM ページ 94 抗菌剤、抗生物質 抗菌剤、抗生物質 10 Antibiotics 10 Antibiotics ナリジクス酸系抗生物質 Nalidixic acid antibiotics ナリジクス酸系抗生物質 Nalidixic acid antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 10. Cefuroxime sodium 11. Cefoxitin sodium 1 Cefadroxil 2 Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 Cephaloridine (A010324A) (A010324C) (O940718B) (O940927B) Column : Hydrosphere C18 (3μm, 12nm) 50 × 2.0 mm I.D. Eluent : acetonitrile / 50mM NaH2PO4 (25/75) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.128 AUFS Injection : 1 μL (0.02 - 0.1 mg/mL) : Hydrosphere C18 (3μm, 12nm) 50 × 2.0 mm I.D. Eluent : 10mM CH3COOH - CH3COONH4(pH 4.3) / acetonitrile (75/25) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.128 AUFS Injection : 1 μL (0.02 - 0.1 mg/mL) Column : J'sphere ODS-L80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2 PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.12 ∼0.35 mg/mL) : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics Column 1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 10. Cefuroxime sodium 11. Cefoxitin sodium 10 抗 菌 剤 ・ 抗 生 物 質 1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 10. Cefuroxime sodium 11. Cefoxitin sodium Column 1 1 Cefadroxil Cefadroxil 2 Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 2 10 Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 Cephaloridine Cephaloridine (O940719A) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.12∼0.35 mg/mL) 94 (O940715F) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2 PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.12 ∼0.35 mg/mL) (O940927C) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) (O940927I) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) 95 アプリケーションデータ集03 01.11.12 0:17 PM ページ 96 抗菌剤、抗生物質 抗菌剤、抗生物質 10 Antibiotics 10 Antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 1 1 Cefadroxil Cefadroxil 2 2 Cefoperazone sodium Cefoperazone sodium Cephalosporin C potassium Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 2 2 3 Cephalothin sodium Cephalothin sodium Cefatrizine propylene glycol 4 3 3 Cephalexin hydrate 5 Cephalexin hydrate Cefamandole sodium 5 Cefamandole sodium Cephaloridine Cephaloridine (O940927G) (O941025E) (O940928R) (O940929D) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) : J'sphere ODS-H80 (4μm, 8nm) 75 × 2.0 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 3 μL (0.17 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Column 1 1 Ceftriaxone sodium 10 10 2 2 2 2 抗 菌 剤 ・ 抗 生 物 質 Cefoperazone sodium Cefoperazone sodium Cefoperazone sodium Cefotaxime sodium Cephalothin sodium Cephalothin sodium Cephalothin sodium 3 3 3 (O940928P) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) 96 Cefamandole sodium Cefamandole sodium Cefamandole sodium (O940928Q) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM NH 4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) (O940928S) Column : YMC-Pack ODS-A (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : THF/50mM NH 4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) (O940928G) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.08, 0.16 mg/mL) 97 アプリケーションデータ集03 01.11.12 0:17 PM ページ 98 抗菌剤、抗生物質 抗菌剤、抗生物質 10 Antibiotics 10 Antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セファロスポリンC Cephalosporin C セフトリアキソン Ceftriaxone 1 1 Ceftriaxone sodium Ceftriaxone sodium 2 2 Cefotaxime sodium Cefotaxime sodium (O940928F) (O940929K) (S000714B) (S000726B) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.08, 0.16 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 2.0 mm I.D. Eluent : methanol/50mM NH4 H2PO4 (30/70) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 3 μL (0.08, 0.16 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 10 μL (0.2 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol / 10mM H3PO4 (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.1, 0.4 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフォキシチンナトリウム Cefoxitin sodium セフォキシチンナトリウム Cefoxitin sodium 1 1 1 10 Caffeine (I.S.) Cephaloglycin Caffeine (I.S.) 2 抗 菌 剤 ・ 抗 生 物 質 Cefoxitin sodium Cefotaxime sodium 10 2 2 Cefoxitin sodium 3 Cephapirin sodium (O940720A) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2 PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.64 AUFS Injection : 5 μL (0.5 mg/mL) 98 (S000717B) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 15 μL (0.1∼0.2 mg/mL) (O940728M) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/50mM KH2 PO4 (20/80) Flow rate : 1.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 μL (8, 12 mg/mL) (O940728P) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 2.0 mm I.D. Eluent : acetonitrile/50mM KH2PO4 (20/80) Flow rate : 0.34 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 μL (1.6, 2.4 mg/mL) 99 アプリケーションデータ集03 01.11.12 0:17 PM ページ 100 抗菌剤、抗生物質 抗菌剤、抗生物質 10 Antibiotics 10 Antibiotics セフォキシチンナトリウム Cefoxitin sodium ペニシリン系抗生物質 Penicillin antibiotics エリスロマイシン Erythromycin antibiotic drugs 1 2 テトラサイクリン系抗生物質 Tetracycline antibiotics Bacampicillin hydrocloride 1.Erythromycin 2.Erythromycin ethylsuccinate 3.Erythromycin estolate 1 1 Methicillin sodium 3 Caffeine (I.S.) 4 Penicillin G potassium (Benzylpenicillin potassium) Oxytetracycline hydrochloride 2 2 5 Penicillin V potassium (Phenoxymethylpenicillin potassium) Tetracycline hydrochloride Cefoxitin sodium Phenethicillin potassium 6 (Phenoxyethylpenicillin potassium) 7 Cloxacillin sodium 8 Propicillin potassium Dicloxacillin sodium (O940729A) (O950908A) (S930910B) (S970524D) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 2.0 mm I.D. Eluent : acetonitrile/50mM KH2PO4 (20/80) Flow rate : 0.34 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 μL (1.6, 2.4 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (67/33) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 8 μL (0.13 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/100mM (NH4)2HPO4(45/15/40) Flow rate : 1.0 mL/min Temperature : 45 ℃ Detection : UV at 210 nm, 0.26 AUFS Injection : 15 μL (2.5 mg/mL) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM oxalic acid (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 6 μL (0.2 mg/mL) ペニシリン系抗生物質 Penicillin antibiotics アモキシシリン Amoxicillin テトラサイクリン系抗生物質 Tetracycline antibiotics バンコマイシン Vancomycin 1 1 Amoxicillin hydrate 2 Oxytetracycline hydrochloride 10 10 2 Ampicillin anhydrous 抗 菌 剤 ・ 抗 生 物 質 Tetracycline hydrochloride (O950920C) (S000728B) (S000622D) : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (80/20) containing 20mM sodium 1-heptanesulfonate Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.13 AUFS Injection : 2 μL (0.3, 0.7 mg/mL) (S970526B) Column 100 Column Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile / water / TFA (10/90/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 10 μL (0.2, 0.1 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM oxalic acid (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 4 μL (0.2 mg/mL) : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2 PO4 - K2HPO4 (pH 6.9) / acetonitrile (88/12) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 10 μL (0.1 mg/mL) 101 アプリケーションデータ集03 01.11.12 0:17 PM ページ 102 農薬、環境関連物質 農薬、環境関連物質 11 Pesticides, Environmental 11 Pesticides, Environmental ベンスリド O,O -Di-isopropyl S-2-benzenesulphonamidoethylphosphorodithioate チウラム Bis(dimethylthiocarbamoyl)disulfide 除草剤 Herbicides アトラジン誘導体 Atrazine derivatives 1. Atrazine-desisopropyl 2. Atrazine-desethyl 3. Crimidine 4. Hexazinone 5. Metoxuron 6. Simazine 7. Cyanazine 8. Atraton 9. Atrazine 10. Isoproturon 11. Diuron 12. Prometon 13. Metobromuron 14. Terbumeton 15. Metazachlor 16. Ametryn 17. Sebuthylazine 18. Propazine 19. Terbuthylazine 20. Linuron 21. Prometryn 22. Terbutryn 23. Metolachlor 1.Thiram 1.Bensulide 1 Atrazine-2-hydroxy 2 Atrazine-desisopropyl 3 Atrazine-desethyl 4 Atrazine (O941018B) Column : YMC-Pack MB-ODS 150 × 2.1 mm I.D. Eluent : acetonitrile/water (60/40) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.01 AUFS Injection : 10μL (1.0 mg/L) Column : YMC-Pack MB-ODS 150 × 2.1 mm I.D. Eluent : acetonitrile/water (40/60) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.01 AUFS Injection : 10 μL (2.0 mg/L) : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4 H2 PO4 (20/80) B)acetonitrile/50mM NH4H2PO4 (65/35) 0-100%B (0-60min, linear) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 5 μL (0.04 mg/mL) アシュラム Methyl sulfanilylcarbamate オキシン銅 Copper 8-quinolinolate トリアジン系除草剤 Triazine herbicides (G910223E) (G910223D) 1.Asulam Column (O941020H) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (25/75/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 5 μL (0.04 mg/mL) トリアジン系除草剤 Triazine herbicides 1. Copper 8-quinolinolate 5 1 1. Cyanazine 2. Simazine 3. Atraton 4. Atrazine 5. Prometon 6. Terbumeton 7. Sebuthylazine 8. Propazine 9. Terbuthylazine 10. Prometryn 11. Terbutryn Simazine Sebuthylazine 6 2 Atrazine 11 Propazine 7 3 Prometon 農 薬 ・ 環 境 関 連 物 質 Terbuthylazine 8 4 Terbumeton Prometryn 9 Terbutryn (G910223C) Column : YMC-Pack MB-ODS 150 × 2.1 mm I.D. Eluent : THF/water/TFA (10/90/0.05) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.01 AUFS Injection : 10 μL (1.0 mg/L) 102 (A010717A) Column : Hydrosphere C18 (5μm, 12nm) 150 × 2.0 mm I.D. Eluent : acetonitrile/20mM H3PO4 (5/95) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 250 nm, 0.32 AUFS Injection : 2 μL (0.02 mg/mL) (O941102F) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH 4H2PO4 (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 5 μL (0.05 mg/mL) (A970210B) Column : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 5 μL (0.05 mg/mL) 103 11 アプリケーションデータ集03 01.11.12 0:17 PM ページ 104 農薬、環境関連物質 農薬、環境関連物質 11 Pesticides, Environmental 11 Pesticides, Environmental 尿素系除草剤 Urea herbicides 酸アミド系除草剤 Acid amide herbicides 1 防かび剤 Fungicides 防かび剤 Fungicides 1 1 1 Metoxuron Metazachlor 2 Thiabendazole Thiabendazole 2 2 2 Isoproturon 3 o-Phenylphenol o-Phenylphenol Metolachlor 3 3 3 Diuron 4 Alachlor Metobromuron Imazalil Imazalil 4 4 4 5 Diphenyl Diphenyl Butachlor Linuron (O941028B) (O941108C) (A970217A) (A970219A) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 4 μL (0.2 mg/mL) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 2-propanol/THF/water (25/15/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 8 μL (0.05 mg/mL) Column : YMC-Pack Pro C18 (5 μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol/20mM NH4 H2 PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 10 μL (0.02∼0.20 mg/mL) : YMC-Pack ODS-A (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 10 μL (0.02 ∼0.20 mg/mL) カルバメート系除草剤 Carbamate herbicides カルバメート系殺虫剤 Carbamate insecticides イマザリル Imazalil アルデヒドおよびケトン類の 2,4-DNPH誘導体 2,4-Dinitrophenylhydrazones of aldehydes and ketones Column 1.Formaldehyde 2,4-DNPH 2.Acetaldehyde 2,4-DNPH 3.Acetone 2,4-DNPH 4.Acrolein 2,4-DNPH 5.Propionaldehyde 2,4-DNPH 6.Crotonaldehyde 2,4-DNPH 7.Methylethylketone 2,4-DNPH 8.Isobutyraldehyde 2,4-DNPH 9.Benzaldehyde 2,4-DNPH 10.n-Valeraldehyde 2,4-DNPH 11.p-Tolualdehyde 2,4-DNPH 12.Capronaldehyde 2,4-DNPH 1.Imazalil 1 Propham 2 Swep 11 11 3 Chloropropham 農 薬 ・ 環 境 関 連 物 質 (T920302A) Column (O941124G) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.16 AUFS Injection : 5 μL (0.08 mg/mL) 104 (J010220D) Column : Hydrosphere C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol / 20mM KH2PO4 (45/55) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 240 nm, 0.64 AUFS Injection : 5 μL (0.1 - 1.5 mg/mL) (B931007A) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM NH4H2PO4 (50/50) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.04 AUFS Injection : 10 μL (0.05 mg/mL) : YMC-Pack C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)tetrahydrofuran/water (10/90) B)acetonitrile 35%B (0-7min), 35-65%B (7-18min, linear), 100%B (18-19min), 35%B (19-35min) Flow rate : 1.5 mL/min Temperature : 30 ℃ Detection : UV at 360 nm, 0.01 AUFS Injection : 11 μL (0.0025 mg/mL) 105 アプリケーションデータ集03 01.11.12 0:17 PM ページ 106 農薬、環境関連物質 その他 11 Pesticides, Environmental 12 The others アルデヒドおよびケトン類の2,4-DNPH誘導体 2,4-dinitrophenylhydrazones of aldehydes and ketones 多環芳香族炭化水素 Polyaromatic hydrocarbons ヒドロキシ安息香酸 Hydroxybenzoic acids 1.Benzene 2.Naphthalene 3.7,12-Dimethylbenzo[a]anthracene 4.Acenaphthylene 5.Diphenyl 6.Fluorene 7.Naphthacene 8.Acenaphthene 9.Phenanthrene 10.Anthrathene 11.Fluoranthene 12.Pyrene 13.Triphenylene 14.Chrysene 15.Perylene 16.Benzo[e]pyrene 17.Benzo[b]fluoranthene 18.Benzo[k]fluoranthene 19.Benzo[a]pyrene 20.Dibenzo[a,h]anthracene 21.Benzo[g,h,i]perylene 22.Coronene 1.Formaldehyde 2,4-DNPH 2.Acetaldehyde 2,4-DNPH 3.Acetone 2,4-DNPH 4.Acrolein 2,4-DNPH 5.Propionaldehyde 2,4-DNPH 6.Crotonaldehyde 2,4-DNPH 7.Methylethylketone 2,4-DNPH 8.Isobutyraldehyde 2,4-DNPH 9.Benzaldehyde 2,4-DNPH 10.n-Valeraldehyde 2,4-DNPH 11.p-Tolualdehyde 2,4-DNPH 12.Capronaldehyde 2,4-DNPH レゾルシン酸異性体 Resorcylic acid isomers 4 1 7 1 3,5-DHBA 2,5-DHBA 5 2 2,3-DHBA 8 α-Resorcylic acid 2 3,4-DHBA 2,4-DHBA 6 3 Benzoic acid 9 β-Resorcylic acid 3 p-HBA m-HBA Salicylic acid Resorcinol DHBA, Dihydroxybenzoic acid HBA, Hydroxybenzoic acid 4 γ-Resorcylic acid (T931130D) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : A)tetrahydrofuran/water (20/80) B)acetonitrile/methanol (90/10) 30-67%B (0-15min, Linear), 30%B (15-25min) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 360 nm, 0.01 AUFS Injection : 15 μL (0.001 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 7 μL Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (5/90/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 285 nm, 0.16 AUFS Injection : 5 μL (0.1 mg/mL) Column 多環芳香族炭化水素 Polyaromatic hydrocarbons 多環芳香族炭化水素 Polyaromatic hydrocarbons レゾルシン酸異性体 Resorcylic acid isomers 安息香酸誘導体 Benzoic acid derivatives (K930918A) 1.Benzene 2.Naphthalene 3.Acenaphthylene 4.7,12-Dimethylbenzo[a]anthracene 5.Diphenyl 6.Fluorene 7.Acenaphthene 8.Naphthacene 9.Phenanthrene 10.Anthrathene 11.Fluoranthene 12.Pyrene 13.Triphenylene 14.Chrysene 15.Perylene 16.Benzo[e]pyrene 17.Benzo[b]fluoranthene 18.Benzo[k]fluoranthene 19.Benzo[a]pyrene 20.Dibenzo[a,h]anthracene 21.Benzo[g,h,i]perylene 22.Coronene 11 農 薬 ・ 環 境 関 連 物 質 (O940530D) 1.Benzene 2.Naphthalene 3.7,12-Dimethylbenzo[a]anthracene 4.Acenaphthylene 5.Diphenyl 6.Fluorene 7.Naphthacene 8.Acenaphthene 9.Phenanthrene 10.Anthrathene 11.Fluoranthene 12.Pyrene 13.Triphenylene 14.Chrysene 15.Perylene 16.Benzo[e]pyrene 17.Benzo[b]fluoranthene 18.Benzo[k]fluoranthene 19.Benzo[a]pyrene 20.Dibenzo[a,h]anthracene 21.Benzo[g,h,i]perylene 22.Coronene (O940607C) : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.05 ∼0.4 mg/mL) 1 1 p-Ethoxybenzoic acid 2 α-Resorcylic acid 2 p-Propoxybenzoic acid 3 β-Resorcylic acid 3 4 Resorcinol p-Isopropylbenzoic acid (Cuminic acid) 4 p-Buthoxybenzoic acid 12 5 γ-Resorcylic acid p-tert-Butylbenzoic acid (K930918C) (K930918B) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 7 μL 106 Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/water (50/50) B)acetonitrile 30%B (0-5min), 30-100%B (5-40min), 100%B (40-50min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 7 μL (O940606J) Column : YMC-Pack Ph (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.4 mg/mL) (O950511B) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (40/60/1) Flow rate : 0.5 mL/min Temperature : ambient (26 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.04 ∼0.2 mg/mL) 107 アプリケーションデータ集03 01.11.12 0:17 PM ページ 108 その他 その他 12 The others 12 The others 安息香酸誘導体 Benzoic acid derivatives メトキシ安息香酸異性体 Methoxybenzoic acid isomers クロロ安息香酸異性体 Chlorobenzoic acid isomers ポリフェノール Polyphenols 1 1 p-Ethoxybenzoic acid o-Chlorobenzoic acid 2 o-Methoxybenzoic acid (o-Anisic acid) 2 1 3 2 p-Propoxybenzoic acid 3 m-Chlorobenzoic acid p-Isopropylbenzoic acid (Cuminic acid) 4 3 p-Methoxybenzoic acid (p-Anisic acid) 4 1.Pyrogallol 2.Hydroquinone 3.Catechol 4.Phenol 2 1 3 p-Chlorobenzoic acid m-Methoxybenzoic acid (m-Anisic acid) p-Buthoxybenzoic acid 5 0 p-tert-Butylbenzoic acid 10 20min (H890627A) (O950511C) (O950508A) (O950509D) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (40/60/1) Flow rate : 0.5 mL/min Temperature : ambient (26 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.04∼0.2 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.17 mg/mL) : YMC-Pack ODS-AM (5 μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : UV at 270 nm, 0.04 AUFS Injection : 10 μL (0.17 mg/mL) Column : YMC-Pack Ph (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 5mM acetic acid Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 280 nm, 0.32 AUFS Sample : reagents Injection : 20 μL メトキシ安息香酸異性体 Methoxybenzoic acid isomers クロロ安息香酸異性体 Chlorobenzoic acid isomers ジメチルフェノール異性体 Dimethylphenol isomers ジメチルフェノール異性体 Dimethylphenol isomers Column 1 1 2 1 2 2,6-Dimethylphenol 2,5-Dimethylphenol 4 1 2,6-Dimethylphenol o-Chlorobenzoic acid o-Methoxybenzoic acid (o-Anisic acid) 3 2,5-Dimethylphenol 4 3 2 2,4-Dimethylphenol 2 5 2,4-Dimethylphenol 2,3-Dimethylphenol 5 6 2,3-Dimethylphenol 6 m-Chlorobenzoic acid 3 p-Methoxybenzoic acid (p-Anisic acid) 3,5-Dimethylphenol 3,4-Dimethylphenol 3,5-Dimethylphenol 3,4-Dimethylphenol 3 p-Chlorobenzoic acid m-Methoxybenzoic acid (m-Anisic acid) 12 12 そ の 他 (O950508B) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.17 mg/mL) 108 (O950509B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : UV at 270 nm, 0.04 AUFS Injection : 10 μL (0.17 mg/mL) (T921019B) : YMC-Pack Polyamine Ⅱ 250 × 4.6 mm I.D. Eluent : 20% ethyl acetate in hexane Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 20 μL (0.33 mg/mL) Column (T921020C) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 10% ethyl acetate in hexane Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 20 μL (0.33 mg/mL) 109 アプリケーションデータ集03 01.11.12 0:17 PM ページ 110 その他 その他 12 The others フェノール誘導体 Phenol derivatives 12 The others フェノール誘導体 Phenol derivatives 1 クレゾール異性体 Cresol isomers 1 o-Nitrophenol 1- (2-ピリジルアゾ) -2-ナフトール(PAN)金属キレート Metal-PAN chelates 1.Co (Ⅱ)-1- (2-Pyridylazo)-2-naphthol 2.Ni (Ⅱ)-1- (2-Pyridylazo)-2-naphthol 3.Fe (Ⅲ)-1- (2-Pyridylazo)-2-naphthol 4.Zn (Ⅱ)-1- (2-Pyridylazo)-2-naphthol 1 2,6-Dinitrophenol 2 2 m-Cresol o-Chlorophenol 2,4,6-Trinitrophenol 3 2,4-Dinitrophenol 4 3 2 m-Nitrophenol 4 p-Cresol 3 p-Nitrophenol 2,6-Dichlorophenol 5 5 o-Cresol m-Chlorophenol 2,4-Dichlorophenol 6 6 p-Chlorophenol 2,4,6-Trichlorophenol (T910520A) (O950616I) (O950614C) (O951025C) Column Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 2-propanol/water/TFA (40/60/0.1) Flow rate : 0.4 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 5 μL (0.04 mg/mL) フェノール誘導体 Phenol derivatives 1 Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (35/65/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.13 AUFS Injection : 4 μL (0.08 mg/mL) : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (18/82) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 10 μL (0.3 mg/mL) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water (70/30) containig 0.01% PAN and 10mM tetrabutylammonium bromide Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : VIS at 565 nm, 0.08 AUFS Injection : 12 μL (25∼92 μg/mL) クレゾール異性体 Cresol isomers 配位性化合物 Coordination compounds ヒノキチオール Hinokitiol o-Nitrophenol Column 1. Uracil 2. Acetylacetone 3. 8-Quinolinol 1 2 1 m-Cresol o-Chlorophenol 3 2 Hinokitiol 2 m-Nitrophenol p-Cresol 4 3 1-Naphthol(I.S.) p-Nitrophenol 5 12 o-Cresol 12 m-Chlorophenol 6 そ の 他 p-Chlorophenol (O950614F) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (35/65/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.13 AUFS Injection : 4 μL (0.08 mg/mL) 110 (O951025B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (18/82) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 10 μL (0.3 mg/mL) (M910604I) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH7.6)/methanol (40/60) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 254 nm, 0.32 AUFS (O960507F) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO 4 containing 1mM EDTA・2Na/acetonitrile (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 320 nm, 0.16 AUFS Injection : 10 μL (0.08, 0.25 mg/mL) 111 アプリケーションデータ集03 01.11.12 0:17 PM ページ 112 その他 その他 12 The others 12 The others アミノアルコール Aminoalcohols 尿素及び関連化合物 Urea and related compounds 1.Ethanolamine 2.Diethanolamine 3.N-Methylethanolamine 4.Triethanolamine 5.N,N-Dimethylethanolamine フラーレン同族体 Fullerenes 1.Urea 2.Propionamide 3.Ethylurea 4.1,3-Diethylurea 5.1,1-Diethylurea フラーレン同族体 Fullerenes 1 1 Buckminsterfullerene(C60) Buckminsterfullerene(C60) 2 2 (5,6)-Fullerene(C70) (5,6)-Fullerene(C70) (M920124B) (M920108A) (S930119B) Column : YMC-Pack PolymerC18 250 × 6.0 mm I.D. Eluent : 100mM Na 2HPO4/100mM NaOH (60/40, pH 12.0) Flow rate : 0.6 mL/min Temperature : 20 ℃ Detection : UV at 215 nm, 0.32 AUFS Injection : 50 μL (0.2∼3.0 mg/mL) Column : YMC-Pack PolymerC18 250 × 6.0 mm I.D. Eluent : 0.4% HClO4 (pH 1.3) Flow rate : 0.5 mL/min Temperature : 25 ℃ Detection : UV at 210 nm, 0.32 AUFS Injection : 15 μL (0.4 ∼7.5 mg/mL) Column 界面活性剤 A nonionic detergent ポリエチレングリコール Polyethyleneglycols 芳香族アミン Aromatic amines 1.Triton X-100 : YMC-Pack ODS-A (5μm, 120A) 150 × 6.0 mm I.D. Eluent : hexane/2-propanol (70/30) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 350 nm, 0.08 AUFS Injection : 4 μL (0.125 mg/mL) 1.PEG #20,000 2.PEG #6,000 3.PEG #4,000 4.PEG #1,000 5.PEG #300 6.Ethyleneglycol (MW 20,000) (MW 8,400) (MW 3,350) (MW 1,000) (MW 300) (MW 62) (S930128B) Column : YMC-Pack Ph (5μm, 120A) 150 × 6.0 mm I.D. Eluent : hexane/2-propanol (50/50) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 350 nm, 0.08 AUFS Injection : 4 μL (0.125 mg/mL) 芳香族アミン Aromatic amines 1 1 p-Phenylenediamine p-Phenylenediamine 2 2 p-Aminophenol p-Aminophenol 3 3 m-Phenylenediamine m-Phenylenediamine 4 4 m-Aminophenol m-Aminophenol 5 5 12 o-Phenylenediamine o-Phenylenediamine 6 6 そ の 他 o-Aminophenol o-Aminophenol (G920109C) (G920123A) : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : acetonitrile/water (10/90) Flow rate : 1.0 mL/min Temperature : ambient (23 ℃) Detection : UV at 260 nm, 0.02 AUFS Injection : 15 μL (5.0 mg/mL) Sample : Triton X-100 (S961007C) (S961004E) Column 112 Column : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : acetonitrile/water(10/90) Flow rate : 1.0 mL/min Temperature : ambient (22 ℃) Detection : RI, 64 x 10-6 RIU/FS Injection : 100 μL (1.67 mg/mL) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4 )2HPO 4 (pH 6.7)/methanol (95/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.26 AUFS Injection : 5 μL (0.125∼0.5 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4 H2 PO4 - (NH4)2 HPO4 (pH 6.7)/methanol (95/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.26 AUFS Injection : 5 μL (0.125∼0.5 mg/mL) 113 12 アプリケーションデータ集03 01.11.12 0:17 PM ページ 114 その他 光学異性体 12 The others 13 Optical isomers フタル酸異性体 Phthalic acid isomers フタル酸異性体 Phthalic acid isomers ビナフトール 1,1'-Bi-2-naphthol トリフルオロアンスリルエタノール 2,2,2-Trifluoro-1-(9-anthryl)-ethanol Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (50/50) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 235 nm Injection : 1 μL (2.8 mg/mL) Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (40/60) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 254 nm Injection : 1 μL (0.14 mg/mL) ヘキソバルビタール Hexobarbital CBZ-フェニルアラニン CBZ-Phenylalanine(Z-Phe-OH) 1 1 Phthalic acid Phthalic acid 2 2 Terephthalic acid Terephthalic acid 3 3 Isophthalic acid Isophthalic acid (A971112B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 3.0 mm I.D. Eluent : methanol/10mM H 3PO4 (25/75) Flow rate : 0.4 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 4 μL (0.05∼0.5 mg/mL) (A971112A) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM H 3PO4 (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.5 mg/mL) 12 そ の 他 13 Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 0.7 mL/min Temperature : ambient Detection : UV at 210 nm Injection : 1 μL (1.2 mg/mL) 114 Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : 0.5M NaClO4 - HClO4 (pH 2.0)/acetonitrile (70/30) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 254 nm Injection : 10 μL (1.5 mg/mL) 115 アプリケーションデータ集03 01.11.12 0:17 PM ページ 116 光学異性体 光学異性体 13 Optical isomers 13 Optical isomers 塩酸プロプラノロール Propranolol hydrochloride 塩酸ケタミン Ketamine hydrochloride フルルビプロフェン Flurbiprofen ヘキソバルビタール Hexobarbital Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/0.5M NaClO 4 (40/60) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 254 nm Injection : 5 μL (1.5 mg/mL) Column Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/0.5M NaClO4 (40/60) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 268 nm Injection : 10 μL (1.4 mg/mL) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (10/90) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (70/30) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) クロルタリドン Chlorthalidone フェノプロフェンカルシウム Fenoprofen calcium salt; hydrate イブプロフェン Ibuprofen インダパミド Indapamide Column 13 光 学 異 性 体 13 : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (10/90) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : water/acetic acid (100/1) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) Column 116 : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.1M AcOHaq - TEAaq (pH 4.0)/acetonitrile (10/90) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 20 μL (2 mg/mL) Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/acetonitrile (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) Column 117 アプリケーションデータ集03 01.11.12 0:17 PM ページ 118 光学異性体 光学異性体 13 Optical isomers 13 Optical isomers ケトプロフェン Ketoprofen CBZ-フェニルアラニン CBZ-Phenylalanine (Z-Phe-OH) フェニルエチルアルコール Phenylethylalcohol 塩酸プロプラノロール Propranolol hydrochloride Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (30/70) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 10 μL (1 mg/mL) Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (40/60) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/methanol (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (10 mg/mL) : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/acetic acid/triethylamine (99/1/0.3/0.25) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) フルルビプロフェン Flurbiprofen ナフチルエチルアルコール 1-(1-Naphthyl)-ethylalcohol トレーガー塩基 Troger's base CBZ-フェニルアラニン CBZ-Phenylalanine (Z-Phe-OH) Column 13 光 学 異 性 体 13 : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/methanol (30/70) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) Column 118 : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/acetonitrile (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (60/40) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column 119 アプリケーションデータ集03 01.11.12 0:17 PM ページ 120 120