2012年度 生物学実験ノート

1
15
7
11
73
11
15
60*15/(7*10) = 90/7
100/15 = 6.7
10
10
60
13
60/(7*10/15) =
10
15
2
12
1
0
1 2
Av ± SEM (n = xxx). SEM standard error of the mean
standard deviation
standard error of mean
±1.0×
±1.96 (
X
Y
Excel2003
Excel2010
X
Excel2003
Y
OK
1
)×
C2-D9
C
A
C
C10-C13
D10
http://bit.ly/euglena3-01
Microsoft
Excel
2003
G1
I9
22043
I9-J15
22043
Y
Y
OK
2
2
X
I19-I76
Y
J19-J76
X
X
Y
Y
X
X
Y
Y
0-60
3
3
2
I3-J3
3
X
I4
I4
Y
G1
A
I4
Excel2003
J4
B
8
5-50
37.6±1.9
n = 16
40
20%
13.5% NaCl
13.3% NaCl 0.9% (0.9% NaCl
14
C
10
10
40
100
100
100
100
100
10
Web
http://bioimaging.jp/learn/035/
http://bioimaging.jp/learn/
http://bioimaging.jp/learn/013/
Tetrahymena pyriformis (GL
Tetrahymena
pyriformis
GL
Euglena gracilis (Klebs Z
strain
microtubule
Essential
2% (w/v)
EDTA
B1
B1
B12
ECB
0.5%
B12
2%
90 µM
5
2
5
flagella
cilia
ECB
PC
)
2011/9/26
Excel2003
Excel2010
ECB17
1 mL
1.26
15
0.2 mL 200
0B
10
10b
10b/a
= 10
=100
80
Tetrahymena
Euglena
70
60
50
40
30
20
10
0
0
10
20
30
40
50
60
70
80
a, b,
V
6
ab 2
0.524ab 2
µm
pL
10-12 L
µL
pL
L
µm
pL
10 cm 3
10-6 m
1000 fL
0.1 m
10-18 m
1000 µm
3
10-3 m3
10-15 L 1 fL
1 mL (1 cc)
87,967,543
8.8
8.8 x 107
0.88
time0
(
8
9
time30
)
; starvation medium
700 g
700
1.0 mM Tricine, 1.6 mM CaCl2, pH 7.0
2 % (w/v)
4500 rpm
1.0 µm
1.0 µm
rate-limiting factor
25 µL(1
50 µL(3
75 µL(2
80 µL(1
2nd
: 1 (0.2%)
4 (0.2%),9 (2%) 5 (2%)
1st
2nd
3rd
R to be Tetra to be
S to be
R stock to
final R (%) be
used (%) added (µL) added (µL) added (µL) total (µL)
time 0
)
1
2
3
4
5
6
7
8
9
100 µL
time x
time 30 (
14.5 µL
10
0.025
0.050
0.075
0.100
0.200
0.250
0.500
0.500
1.000
0.2
0.2
0.2
0.2
2.0
2.0
2.0
2.0
2.0
40
20
30
[
]
(
75
50
25
0
80
75
50
50
0
25
50
75
100
20
25
50
50
100
100
200
10
0.5%
cytosis
exocytosis
5
(membrane) transport
endocytosis
pinocytosis
endosymbiosis
ECB15
phagocytosis
ECB3
ECB4
10
- 273
10
11
A,B,C,,,
18, 22, 25, 27, 30, 33, 36, 39
[
]
2%
time 0
100 µL
)
100 µL
50 µL
2% R
5 µL
time 30 (
10 µL
30
[
]
(
)
9
10
IF50
J/m2); median Inhibition Fluence
11
(UV; ultraviolet light)
nm), UVA (320-400 nm)
UVC (200-280 nm), UVB (280-320
UVB UVA
UVB
UVC
12
254.4 nm
UVC
; contamination)
12
TLV
1
JIS Z 8812
60 J/m2
8
(Panasoinic
)
0.6
clear west
cloudy south
UVC lamp
UVB lamp
13
mol/m2/s
0.4
260 nm
0.2
254.4 nm
0.0
200
inactivation
250
300
350
400 450
500
550
600
650
700
750 800 850
Wavelength (nm)
UVC
0.20
UVC
0.18
UVC
2
PAR: 5.4 E/m /s
0.16
0.14
0.12
0.10
0.08
0.06
Beer
0.04
0.02
0.00
T=10
cd
200
250
300
350
400 450
500 550
600 650
700 750
800 850
Wavelength (nm)
A=
logT= cd
1 cm
5 cm
UVB
UVB
14
(m)
E = Nhc/
mol
E = 120a/ (J)
1 mol
470 kJ = 112 kcal
(nm)
254.4 nm
200-800 nm
a ( mol)
mol
0.47 J
13
15
UV-C
16
C
9
10
1
2
R
3
1
100 µL
1.0 W/m2
1.0 mL
2
0
2
300 J/m
180 J/m
0
300
10 mM
100 µL
8
0, 30, 60, 90, 120, 180, 300 (±Asc) J/m2
2% R
20
100 µL)
50 µL
time 20
10 µL
5 µL)
0 J/m2, 300 J/m2 (±Asc)
[
time 0
30
]
R2)
ECB2
11
50%
DNA
ECB6
ECB14
14
LD50
J/m2);
median Lethal Dose
11
2.0 W/m2
8.5 10 12 15
0, 840, 1020, 1200, 1440, 1800 J/m2
NR (Neutral-Red
0.3%
0.03%
5
1 mL
7
15
100 µL
100 µL
10 µL
time-15
0.3% NR
15
7 8.5
75
10 12 15
5 µL)
75
time-45
45
75
100×v/tc
J/m2
LD50
50%
15
12
50%
EC50
mM); median Enhancing Concentration
12
11
Asc
50 mM
2.0 W/m2 (J/m2/s) 15
1800 J/m2
Asc
Asc CC(
0(0 mM), 1(0.01 mM), 2(0.1 mM), 3(1 mM), 4(5 mM),5(10 mM), 6(20 mM), 7(50 mM)
CC
75
8
1 mL
15
10 µL
3
100 µL
0.3% NR
time-30
5 µL)
30
75
75
100×v/tc
Asc
linear scale
0.01
Asc
mM EC50
50
CC