目次 コンファレンスプログラム 2 発表演題及び講演時間

MAP
1
2011
11
16
(
)-17
1-1-1
11 月 16 日（水）
11:00 11:55 - 12:00
12:00 - 15:00
O-1
15
15:00 - 15:10
15:10 - 16:40
16:45 - 17:45
17:45 - 18:00
18:30 -
1
11 月 17 日（木）
9:25-12:00
S-1
S-5
12:00-13:00
13:00-14:30
14:30-16:42
O-16
26
16:42-17:00
17:00-17:30
2
(
)
発表演題および講演時間
特別講演
11 月 16 日（水）16:45 - 17:45
「酵母から見えて来たオートファジーの分子機構とその生理機能」
シンポジウム
11 月 17 日（木） 9:25 ‒ 12:00
「糸状菌・キノコの形態形成の意義とその分子機構」
9:25- 9:30
9:30-10:00
S-1
­
­
10:00-10:30
S-2
10:30-11:00
S-3
11:00-11:30
S-4
­
­
11:30-12:00
S-5
3
O-1
12:00
O-1
O-15
11
A. oryzae
16
12:00 - 15:00
MAP
AoFus3
Özgür Bayram1
Oliver Valerius1
Gerhard H. Braus1
1
12:12
O-2
Aspergillus nidulans
12:24
O-3
Aspergillus nidulans
NO
P450
C
,
12:36
O-4
Aspergillus nidulans
-1,3-
agsB
agsA, agsB
12:48
O-5
13:00
O-6
13:12
O-7
13:24
O-8
Epichloë festucae
A. oryzae
G
Cdc42
SclR
1
,
1
,
(
,
,
13:36
O-9
HECT
13:48
O-10
XlnR
,
,
1
,
)
(hulA)
,
,
,
14:00
O-11
Trichoderma reesei
,
14:12
O-12
14:24
O-13
14:36
O-14
14:48
O-15
,
Magnaporthe grisea
MoCV1
LAC2
Cryphonectria parasitica RAS3
4
RacA
O-16
14:30
O-16
14:42
O-17
O-26
11
17
14:30 – 16:42
A. oryzae
Fusarium asiaticum
*
14:54
O-18
*
laeA
Cordyceps militaris
1
Rina
Rachmawati1
1
15:06
O-19
15:18
O-20
15:30
O-21
15:42
O-22
O-23
16:06
O-24
Aspergillus oryzae
RolA
Trichoderma reesei
Aspergillus nidulans
2
1
2
1,2
1
2
2
2
2
O-25
1
(1
16:30
Hydrophobin
(QCM)
1,2
16:18
1
2
1
15:54
2
1, 2 ,3
1
2, 3
2
3
O-26
5
)
2
1, 2
11
11
P-1
Aspergillus awamori
1
15:10 - 16:40
13:00 - 14:30
ligD
1
,
P-2
16
17
2
,
1
,
1
,2
Cre-loxP
*
*
P-3
1
P-4
1
2
1
2
Coleophoma empetri F-11899
P-5
P-6
,
P-7
,
Aspergillus kawachii IFO 4308
1
1
1
P-8
,
1
1
2
1
SOLiD3
1
2
2
1
2
2
RNA-seq
1
1
2
,
1
,
P-9
VHH
1
1
1
,2
Aspergillus oryzae
2
1
2
3
3
1
1
P-10
2
A. oryzae
1
P-11
P-12
3
2
1
1
1
1
2
3
1
1
2
A. oryzae
1
1
2
Aspergillus niger
1
1
3
oxaloacetate hydrolase
P-13
P-14
6
1
(oahA)
P-15
1,2
1,2
2
2
1, 2
2
2
1
2
P-16
P-17
1,2
1,2
(1
2
2
P-18
2
porphobilinogen deaminase
,
P-19
P-22
Aspergillus nidulans
,
nitrosative stress
,
AoSO
, Cristopher Sarazar ESCAÑO,
P-21
2
)
,
P-20
1,2
Aspergillus nidulans
,
ypdA
Aspergillus nidulans
A. oryzae
PhkA,PhkB
MAP
AoFus3
Özgür Bayram1
Oliver Valerius1
Gerhard H. Braus1
1
P-23
P-24
Aspergillus fumigatus
Neurospora crassa
,
P-25
MAK-1
,
MAK-2 MAP
,
Aspergillus nidulans
C
,
P-26
Aspergillus oryzae
P-27
A. oryzae
P-28
A. oryzae
P-29
A. oryzae
AoApsB
A2
Ca2+
AoPlaA
A2
AipC
7
iPlaA
AipD
P-30
(
)
P-31
Aspergillus nidulans
-1,3-
agsB
agsA, agsB
1
2
,
P-32
1,2
,
1
,2
Aspergillus nidulans
CsmA
P-33
Aspergillus nidulans
P-34
Aspergillus nidulans
Saccharomyces cerevisiae CRH
Saccharomyces cerevisiae SKT5
AN3445
P-35
P-36
A. oryzae
Aoatg11
Magnaporthe oryzae
1) 3)
1)
1)
2) 3)
2
1) 3)
1) 3)
3)
P-37
CcAtg8
P-38
Coprinopsis cinerea
1
2
2
,
1
,
1
,2
P-39
cag1
P-40
Epichloë festucae
G
Cdc42
RacA
P-41
1
3
1
1
1
4
1
2
3
4
A. oryzae KBN630
Aph
1
1
3
1
P-43
P-44
2
1
2
P-42
1
2
Aspergillus oryzae
(QCM)
3
Hydrophobin
RolA
8
1
2
P-45
hydrophobin
1
Fv-hyd3
1
1
1
2
1
1
2
P-46
KexA
P-47
serine-type carboxypeptidase
P-48
Aspergillus nidulans
β
P-49
1
1
,
2
,
3
1
,
3
,
4
,
,
1
,
1
2
P-50
2
,
3
4
Phanerochaete chrysosporium
P-51
P-52
A. oryzae
SclR
1
,
P-53
P-54
1
,
,
Aspergillus nidulans
NmrA
AreB
,
,
,1
(
)
Aspergillus oryzae
P-55
Epichloë festucae
1
Scott
P-56
,
2
Sanjay Saikia2
1
Gemma Cartwright2
2
Massey Univ.
ProA
1
3
1
3
Aspergillus nidulans AmyR
,
,
P-57
Aspergillus aculeatus ace1
P-58
Aspergillus aculeatus Zn(II)2Cys6
CGAF
P-59
P-60
XlnR
,
,
,
,
9
,
Barry
P-61
Trichoderma reesei
,
P-62
P-63
,
Trichoderma reesei
Trichoderma reesei
ClbR
1,2
1
,
1
,
1
,
4
,4
DNA
,
, 2 JST, 3
P-64
3
3
,
1
,
1
,
AOEXE103
P-65
P-66
P-67
ABC
A. nidulans
AtrR
AmyR
P-68
,
P-69
Aspergillus niger NRRL 328
P-70
A. oryzae
csyB
1
1
P-71
III
III
1
,
1
2
1
,2
Pyripyropene
Pyr1
,
P-72
terretonin
1
1
P-73
1
2
1
1
2
2,
P-74
peroxide
Verruculogen
1
2
1
1
FtmF
1
2
P-75
1, 2
1
P-76
1
1
1
2
1
2
Coleophoma empetri F-11899
FR901379
10
T-DNA
1
P-77
(
P-78
)
CBP1,CBL1
P-79
B51
P-80
(
P-81
)
DNA
*
*
P-82
MoCV2
P-83
MoCV1
MoCV1
P-84
MoCV3
(Magnaporthe oryzae chrysovirus 3)
P-85
Magnaporthe grisea
P-86
Phomopsis sp. (Diaporthe sp.)
P-87
(Cochliobolus heterostrophus)
NADPH Oxidase
*
*
P-88
Cdc42
,
,
P-89
NIS1
P-90
P-91
LAC2
Cryphonectria parasitica RAS3
P-92
,
P-93
A. oryzae
,
AoSO
,
Stress Granule
11
(
)
/
1.
Atg
23
(atg)
ATG
Atg1
2.
PI3
Atg
Atg
Atg
Atg
PAS
Atg
PAS
Atg
PAS
PAS
PAS
TOR
2 2 2
Atg1-Atg13
Atg9
Atg13
Atg17
Atg1
Atg29
PAS
Atg31
Atg
Atg9
40-60nm
12
Atg9
PAS
Atg9
Atg9
Atg9
Atg9
PAS
PAS
Starvation signal
Isolation
membrane
Vacuole
Autophagosome
Formation
Disintegration
and Digestion
Autophagic
body
Autophagosome
.1
.2
PAS
Atg
13
.3
3.
pH
atg
atg
ROS
ρ
mtDNA
ρ
Atg32
Ty
in vitro
Lessons from Yeast –dissection of molecular mechanism of autophagic machinery Yoshinori Ohsumi（Professor, Frontier Research Center, Tokyo Institute of Technology）
14
S-1
—
—
(
)
Aspergillus oryzae
”
“
(gap
junction)
(plasmodesmata)
A. oryzae
Woronin body
Woronin
body
A. oryzae
1)
2
A. oryzae Woronin body
Woronin body
AoHex1
AoHex1
β
2)
AoHex1
Woronin
body
AoPex11-1
Woronin body
3)
Woronin body
AoSO
4)
AoSO
AoSO
1195
WW
5)
MAPK
6)
AoFus3
AoFus3
15
MAPK
AoFus3
AoApsB
AoFus3
(microtubule-organizing center)
7)
AoSO
8)
2.
3.
4.
5.
6.
7.
8.
9.
J. Maruyama et al. (2005) Biochem. Biophys. Res. Commun. Vol. 331, 1081-1088.
Y. Tanabe et al. (2011) J. Biol. Chem. Vol. 286, 30455-30461.
C. S. Escaño et al. (2009) Eukaryot. Cell Vol. 8, 296-305.
J. Maruyama et al. (2010) Biochem. Biophys. Res. Commun. Vol. 391, 868-873.
2011
p.48
2010
p. 54
2011
p. 51
2011
p. 26
Molecular biological lessons about multicellular organization from septal-pore mediated
intercellular communication in filamentous fungi
Jun-ichi MARUYAMA (Dept. of Biotechnol., Univ. of Tokyo)
16
S-2
Aspergillus oryzae
ku70
ku70
7
I.
:
A. nidulans
G-Protein
flbA
fadA
A. nidulans
A. oryzae
A. nidulans
G-protein
1)
DNA
MED6
II.
7
helix
RNA
:
helix-loopAO090011000215
2)
1) Ogawa M., et al., Fungal Genet. Biol., 47: 10-18 (2010).
2) Jin F.J., et al., Appl. Environ. Microbiol., 75: 5943- 5951 (2009).
Analysis of regulatory systems for conidial and hyphal developments using comprehensive
gene disruption methods in Aspergillus oryzae
Masahiro Ogawa, Feng Jie Jin, and Yasuji Koyama (Noda Institute for Scientific Research)
17
S-3
(Colletotrichum orbiculare)
(2,3,6,7,8)
Kelch
Kelch
CoKEL1, CoKEL2
CoKEL1
CoKEL2
(4,5)
TEA1
CoKEL2
(1) PEX13
PEX5
pex13
acetyl-CoA
acetyl-CoA
PEX5
PEX22
PEX22
Woronin Body(WB)
WB
WB
18
pex22
pex13
104-T (MAFF240422)
91Mb
graminis)
graminicola
(Blumeria
C.
57Mb 53Mb
C. higginsianum
AT
B. graminis
AT
64
1) Fujihara, N., Sakaguchi, A., Tanaka, S., Fujii, S., Tsuji, G., Shiraishi, T., O’Connell, R., and
Kubo Y. (2010) Peroxisome biogenesis factor PEX13 is required for appressorium-mediated
plant infection by the anthracnose fungus, Colletotrichum orbiculare. Mol. Plant-Microbe
Interact. 23: 436-445.
2) Kubo, Y. (2011) Appressorium function in Colletotrichum orbiculare and prospect for genome
based analysis. in Morphogenesis and Pathogenicity in Fungi Series: Topics in Current
Genetics, vol. 22 Pérez-Martín, José; Di Pietro, Antonio (Eds.), Springer. (in press)
3) Kubo, Y., and Tanaka, S. (2010) Pathogenesis and plant basal resistance in Colletotrichum
orbiculare and Magnaporthe oryzae infection. In Genome-Enabled Integration of Research in
19
4)
5)
6)
7)
8)
Plant Pathogen Systems. (Wolpert, T., Shiraishi, T., Allen, C., Glazebrook, J. and Akimitsu, K.
eds), APS Press.
Sakaguchi, A. Miyaji, T. Tsuji G. and Kubo Y. (2008) Kelch-repeat protein Clakel2p and
calcium signaling control appressorium development in Colletotrichum lagenarium. Eukaryot.
Cell 7:102-111.
Sakaguchi, A., Miyaji, T., Tsuji, G., and Kubo, Y. (2010) A Kelch repeat protein Cokel1p
associates with microtubules and is involved in appressorium development in Colletotrichum
orbiculare. Mol. Plant-Microbe Interact. 23: 103-111.
Sakaguchi, A., Tsuji, G., and Kubo, Y. (2010) A yeast STE11 homologue CoMEKK1 is essential
for pathogenesis-related morphogenesis in Colletotrichum orbiculare. Mol. Plant-Microbe
Interacti. 23:1563-1572.
Tanaka, S., Ishihama, N., Yoshioka, H., Huser, A., O'Connell, R., Tsuji, G. ,Tsuge, S., and Kubo
Y. (2009) The Colletotrichum orbiculare ssd1 mutant enhances Nicotiana benthamiana basal
resistance by activating a mitogen-activated protein kinase pathway. Plant Cell 21:2517-2526.
Tanaka, S., Yamada, K., Yabumoto, K., Fujii, S., Huser, A., Tsuji, G., Koga, H., Dohi, K., Mori,
M., Shiraishi, T., O’Connell R., and Kubo, Y. (2007) Saccharomyces cerevisiae SSD1
orthologues are essential for host infection by the ascomycete plant pathogens Colletotrichum
lagenarium and Magnaporthe grisea. Mol.Microbiol. 64: 1332–1349.
Infection structure development and pathogenesis of Colletotrichum orbiculare
Yasuyuki Kubo (Lab. Plant Pathol. Grad. Sch. Life and Environment. Sci., Kyoto Prefectural
University)
20
S-4
1
1
1
2
2
2
1
2,3
3
JAXA
100
1970
1.
3D
3D-
X-Y
2.
3D-
21
3.
mRNA
RDA
cDNAPCR
36
17
RNA
19
TFIIAγ
4.
2
subtilisin
Aspergillus
subtilisin
2D(
22
)-
5.
(stem cell)
[1] Miyazaki Y, Sunagawa M, Higashibata A, Ishioka N, Babasaki K, and Yamazaki T.
Differentially expressed genes under simulated microgravity in fruiting bodies of the fungus
Pleurotus ostreatus. FEMS Microbiol. Lett., 307, 72-79 (2010).
Mushroom formation affected by gravity.
-The gene expression under simulated space environmentYasumasa Miyazaki1, Masahide Sunagawa1, Akira Higashibata2, Noriaki Ishioka2, Katsuhiko
Babasaki1, Takashi Yamazaki2,3
(1Dept. Appl. Microbiol., FFPRI; 2Inst. Space Astronaut. Sci., JAXA; 3(Present address) Inst. Med.
Mycol.,Teikyo Uinv.)
23
S-5
(Coprinopsis cinerea)
[1, 2]
37 Mb
13
[4]
[3]
000
(n)
(n+n)
(2n)
(n+n)
A
A
B
B
(HD1, HD2)
A
B
B
num1
A
ich1
eln2, eln3
dst1, dst2 (wc-1 homolog), Cc.wc2
restriction enzyme-mediated integration (REMI)
24
Cc.ubc2,
clp1 pcc1
exp1
DNA
log2: >2
82
log2: <-2
34
fasciclin, galectin, lectin, hyrophobin
10
Super SAGE
chromatin immunoprecipitation (ChIP)-sequencing
[1] U. Kües (2000). Life history and developmental processes in the basidiomycete Coprinus
cinereus. Microbiol. Mol. Biol. Rev. 64, 316–353.
[2] T. Kamada (2002) Molecular genetics of sexual development in the mushroom Coprinus
cinereus. BioEssays 24: 449-459.
[3] J. E. Stajich et al. (2010) Insights into evolution of multicellular fungi from the assembled
chromosomes of the mushroom Coprinopis cinerea (Coprinus cinereus). Proc. Natl. Acad. Sci.
USA 107, 11889-11894.
[4] T. Nakazawa, Y. Ando, K. Kitaaki, K. Nakahori, T. Kamada (2011) Efficient gene targeting in
ΔCc.ku70 or ΔCc.lig4 mutants of the agaricomycete Coprinopsis cinerea. Fungal Genet. Biol.
48, 939-946.
Analysis of molecular mechanisms for multicellular morphogenesis of the homobasidiomycete
Coprinopsis cinerea
Takashi Kamada (Grad. Sch. Natural Science and Technology, Okayama Univ.)
25
O-1 (P-22)
A. oryzae
MAP
AoFus3
Özgür Bayram1
Oliver Valerius1
Gerhard H. Braus1
1
MAP
Saccharomyces cerevisiae
MAP
Fus3p
Fus3
A. oryzae
Fus3p
Aofus3
AoFus3
AoFus3
Fus3p
A. oryzae
AoFus3
AoFus3
C
Aofus3
TAP(Tandem Affinity Purification)
LC/MS/MS
2
AoFus3
RACE
EGFP
2
AoFus3
Identification and functional analysis of novel AoFus3(MAP kinase)-interacting proteins in Aspergillus oryzae
Daiki YAHAGI, Jun-ichi MARUYAMA, Özgür BAYRAM1, Oliver VALERIUS1, Gerhard H. BRAUS1, Katsuhiko
KITAMOTO (Dept. of Biotechnol., Univ. of Tokyo,1Georg-August-Universität Göttingen)
O-2
Aspergillus nidulans
NO
P450
nitric oxide (NO)
NO
NO
Fzf1p
100
NO
Yhb1p
NO
A. nidulans
NO
DNA
A. nidulans
P450
NO
NO
NO
NO
NO
NO
P450
NO
P450
GC-MS
ESI-MS
nitrated oleic acid (OA-NO2)
NO
NO
nitrated linoleic acid (LA-NO2)
P450
P450
OA-NO2
OA-NO2
P450
NO
New fungal cytochrome P450 tolerates nitrite oxide.
Motoyuki Shimizu, Toshihisa Narukami, Yosuke Kamimura, Shunsuke Masuo, Tatsuya Kitazume, Yasunobu
Terabayashi, Momoko Machida, Naoki Takaya (Graduate School of Life and Environmental Sciences, University of
Tsukuba)
26
O-3 (P-25)
Aspergillus nidulans
C
,
Aspergillus nidulans
DNA
C PKC
PKC
pkcA
42
,
2)
pkcA
ROS
1)
pkcA
30
, 42
pkcA
,
DNA
,
42
, DNA
,
,
PkcA
PkcA
MAP
,
MAP
bckA, mpkA
42
, DNA
,
, ROS
,
,
BckA, MpkA
42
PkcA
MAP
1) Ichinomiya, M., et al., (2007) Biosci. Biotechnol. Biochem., 71, 2787-2799
2)
,
2010
, p213
Analysis of PkcA function in the suppression of apoptosis under heat stress condition in Aspergillus nidulans
Takuya Katayama, Hiroyuki Horiuchi and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)
O-4 (P-31)
Aspergillus nidulans
agsA, agsB
1
2
,
-1,3-
1,2
,
agsB
1
,2
, Aspergillus nidulans
, MAP
,
AGS
AGS
MpkA
,
agsA, agsB
, agsA
, agsB
, agsA
AgsB
1,3AGS
agsB
agsB
MpkA
-1,3,
AGS
,
, agsB
,
2
-
, agsB
, Congo Red
,
, agsB
agsB
, agsA
agsB
,
, NMR
-1,3-
, Aspergillus
-1,3AGS
,
Analysis of the disruptant of
-1,3-glucan synthase gene agsB and the double disruptant of agsA and agsB in
Aspergillus nidulans
Azusa Inaba 1, Akira Yoshimi 2, Keietsu Abe 1,2
( 1 Grad. Sch. Agric. Sci., 2 Tohoku, Univ., NICHe.,Tohoku Univ.)
27
O-5
A Lentinula edodes laccase (Lcc1) influences their mycelial morphology
Keiko Nakade and Yuichi Sakamoto (IBRC)
O-6 (P-40)
Epichloë festucae
G
Cdc42
RacA
E. festucae
E. festucae
NoxA
G
RacA
NoxR
NoxA
BemA/Cdc24
RacA
NoxR
RacA
Cdc42
Nox
RacA
NoxR
Cdc42
Yeast two hybrid
BemA
racA
N
cdc42
racA
cdc42
cdc42
Cdc42
Cdc42
RacA
Functional analysis of small G proteins Cdc42 and RacA from fungal endophyte Epichloë festucae
Yka Kayano, Daigo Takemoto
(Grad. School Bioagr. Sci., Nagoya Univ.)
28
O-7
24
305
H2A, H2B, H3, H4
85
7
9
1
1
2
(1) Yun C-S, Nishida H (2011) PLoS ONE 6: e16548. (2) Nishida
H, Yun C-S (2011) Mobile Genetic Elements 1: 78-79.
Evolution of introns in fungal histone genes
Hiromi Nishida, Choong-Soo Yun
(Graduate School of Agricultural and Life Sciences, Univ. Tokyo)
O-8 (P-52)
A. oryzae
SclR
1
,
1
,
,
,
A. oryzae
,1
(
)
,
,
,
,A. oryzae
(sclerotium)
,
,
(perithecium)
,
,
,
A. oryzae
,
,
,
sC
/
(ΔpyrG)
(ΔadeB)
,
,
,
,
,
,A. oryzae
,
SclR
,
Analysis of hyphal fusion ability by using the strains overexpressing SclR, a promoting factor for sclerotia
formation in Aspergillus oryzae
Ryuta WADA, 1Feng Jie JIN, 1Yasuji KOYAMA, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO
(Dept. of Biotechnol., Univ. of Tokyo, 1Noda Ins. Sci. Res.)
29
O-9
HECT
(hulA)
CreA
Aspergillus nidulans
CreA
CreA
HECT
HulA
HulA
hulA
RSP5
hulA
hulA
nmtA
5´hulA
HulA
HulA
Construction of the conditional expression strain for HECT ubiquitin ligase (hulA) in Aspergillus oryzae.
Mizuki Tanaka, Takahiro Shintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ., Univ.)
O-10 (P-60)
XlnR
,
,
,
Aspergillus oryzae
,
,
XlnR ,
, XlnR
,
,XlnR
phosphate affinity SDS-PAGE
XlnR
DNA
,
c-myc
XlnR
phosphate affinity SDS-PAGE
,
XlnR
c-myc::XlnR
3-4
,
2
,
XlnR
EMSA
,
,XlnR
DNA
ChIP Assay
XlnR
XynF1
DNA
,
,
Inducer-dependent phosphorylation of the transcriptional activator XlnR
and its physiological role in
Aspergillus oryzae
Shuhei Ishikawa, Yuji Noguchi, Kyoko Kanamaru, Masashi Kato , Tetsuo Kobayashi (Graduate Sch. of Bioagric. Sci.,
Nagoya Univ.,
Sch. Agric. Sci., Meijo Univ.)
30
O-11 (P-61)
Trichoderma reesei
,
,
Trichoderma reesei
T.reesei
9129
Functional analysis of genes encoding a putative cello-oligosaccharide transporter in the filamentous fungus
Trichoderma reesei.
Hideyuki Kusaka, Takanori Furukawa, Eiji Fukaya, Yousuke Shida, Wataru Ogasawara
(Deot. Bioeng., Nagaoka Univ. of Tech)
O-12 (P-85)
Magnaporthe grisea
1
BJ-AFP1
Magnaporthe grisea
(ROS)
BJ-AFP1
M. grisea
Pichia pastoris
BJ-AFP1
M. grisea
ROS
15
ROS
BJ-AFP1
15
BJ-AFP1
BJ-AFP1
BJ-AFP1
BJ-AFP1
BJ-AFP1
BJ-AFP1
The mechanism of action of the plant defensin BJ-AFP1 in Magnaporthe gisea
Yoshifumi Oguro, Harutake Yamazaki, Masayuki Nashimoto, Masamichi Takagi, Hiroaki Takaku
(Applied Life Sci, Niigata Univ. of Pharmacy and Applied Life Science)
31
O-13 (P-83)
MoCV1
Magnaporthe oryzae chrysovirus 1(MoCV1)
4~5
RNA
MoCV1
MoCV1
MoCV1
20
MoCV1
2
SDS-PAGE
14
MoCV1
Functional analysis of viral proteins of MoCV1 (Magnaporthe oryzae chrisovirus 1) which confers hypovirlence
traits to rice blast fungus.
Tokomo Ohta, Syunichi Urayama, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu Moriyama.
(Dept. Agric., Tokyo Univ. of Agric. & Tech.)
O-14 (P-90)
LAC2
Colletotrichum orbiculare
LAC2
LAC2
GFP
LAC2
LAC2
lac2
lac2
Magnaporthe oryzae
LAC2
lac2
LAC2
LAC2
LAC2
Isolation and Functional Analysis of a Secreted Protein Gene LAC2 Required for Fungal Pathogenicity of
Colletotrichum orbiculare
ShaoYu Lin, Shiho Okuda, Tetsuro Okuno, Yoshitaka Takano
32
(Grad. School of Agriculture, Kyoto Univ.)
O-15 (P-91)
Cryphonectria parasitica RAS3
chestnut blight
American chestnut
Cryphonectria parasitica
1900
C. parasitica
C. parasitica
RAS3
C
Rin
Rit
RAS
RAS1
RAS2
RAS3
Ras
CAAX Box
RAS3
ras3
GFP
RAS3
C
32
RAS3
C
32
RAS3
GTP
GTPase
RAS3
Biochemical and physiological functions of Cryphonectria parasitica RAS3
Yuki Yamauchi, Takuya Takahashi and Shin Kasahara
(Dept. of Env. Sci., Miyagi Univ.)
O-16
A. oryzae
Aspergillus oryzae
A. oryzae
PKS
Zn
DNA
amyB
PKS
NMR
in vitro
Activation of a silent secondary metabolic gene cluster by upregulating a transcriptional regulator in A. oryzae
Takehito Nakazawa, Kan’ichiro Ishiuchi, Kenji Watanabe
(School of Pharmaceutical Sciences,Univ. of Shizuoka)
33
O-17
Fusarium asiaticum
*
*
Fusarium asiaticum
F. asiaticum10
11-
1F. asiaticum
2,1-
F. asiaticum
Effects of carbon sources on trichothecene production and Tri gene expression by Fusarium asiaticum.
Akira Kawakami, *Takashi Nakajima, and Kazuyuki Hirayae (Kyushu Okinawa Agricultural Research Center, *Food
Safety Commission)
O-18
laeA
Cordyceps militaris
1
Rina
Rachmawati1
2
1
1
2
Cordyceps militaris
C. militaris
LaeA
Aspergillus
LaeA
C. militaris
LaeA
Beauveria bassiana
A. nidulans
laeA
PEG
C. militaris
HPLC
gpd
1
H-NMR
EI-MS
Beauvericin
Activation of secondary metabolism of Cordyceps militaris by heterologous expression of global regulator
Hiroshi Kinoshita1, Rina Rachmawati1, Fumio Ihara2, Takuya Nihira1
(1ICBiotech, Osaka Univ, 2NIFTS)
34
O-19 (P-43)
Aspergillus oryzae
Hydrophobin
Hydrophobin
70~150a.a.
Hydrophobin
Aspergillus oryzae
hypA,B,C,D
hypA,B,C
HypA
HypB,C
hypA, B, C
Hydrophobin
4
HypA,B,C
Hydrophobin
HypA
PCR
SEM
A. oryzae
HypD
Hydrophobin
Functional analysis of Hydrophobins by multiple gene disruption in Aspergilllus oryzae
Yui Yamakawa, Yuka mizuno, Harushi Nakajim
(Dept. of Agricultural Chemistry, Univ. of Meiji)
O-20 (P-44)
(QCM)
PBSA
RolA
CutL1
hydrophobin
PBSA
PBSA
RolA
PBSA
Polybutylene succinate-co-adipate
cutinase CutL1
hydrophobin RolA
PBSA
CutL1
pH
RolA
RolA
RolA
PBSA
RolA
PBSA
PBSA
L137S, L142S, D134A/D136A,
RolA
RolA
RolA
K130A/D134A/D136A
PBSA
PBSA
PBSA
QCM
RolA
1) Takahashi et al., Mol Microbiol. 57: 1780-1798 (2005)
QCM analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Hiroki Tanabe 1, Keiko Orui 1, Kenji Uehara 1, Toru Takahashi 2, 3, Takanari Togashi 4, Toshihiko Arita 4,
Keietsu Abe 1, 2
(1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 NRIB., 4 IMRAM., Tohoku Univ.)
35
O-21
Trichoderma reesei
Trichoderma reesei
T. reesei
T. reesei
T. reesei
T.
reesei
RNA
Morphological analysis of cellulolytic fungus Trichoderma reesei
Yosuke Shida1, Mikiko Nitta1, Masako Osumi2, Wataru Ogasawara1
(1Nagaoka Univ. of Thecnol, 2Integr. Imaging Res Support)
O-22
Aspergillus nidulans
1
2
1
2
(DR)
(IR)
IR
25%
(illegitimate)
(NHEJ)
(homologous)
IR
5-10
IR
(ectopic)
85%
15%
IR
DNA
NHEJ
Effective gene targeting by addition of excessive non-homologous fragments in Aspergillus nidulans
Yasuo Itoh1, Ayana Kon2 (1Sch. Gen. Ed., 2Fac. Sci. / Shinshu Univ., Present affiliation: Frontier Sci. Univ. of Tokyo)
36
O-23
,
,
,
,
,
PEG
,
,
URA3
2µ Ori
,
,
,
,
,
Aspergillus nidulans
PyrG
Cre
, Cre-loxP
,
Comprehensive toolkits for fungal omicses: Versatile modules for gene manipulation regarding replacement,
expression, targeting, and tagging.
Toshiaki Harashima, Marie Nishimura
(NIAS)
O-24 (P-15)
1,2
1,2
2
2
2
2
1, 2
1
2
55
9
55
40%
A40Y
(A40Y)
60%
(S60Y)
S60Y
Evolution of industrial Aspergillus oryzae genome and its adaptation to the fermentation industries
Gaku Ito1, 2, Yuhei Senoo1, 2, Tuneko Sawamura2, Masahumi Tokuoka2, Atsuko Isogai2, Osamu Yamada2, Kazuhiro
Iwashita1, 2
(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB)
37
O-25
1)
1)2)3)
1)
2)3)
( 1)
2)
3)
)
EST
EST
56
187
Gf.HSP9
C2H2
zinc finger
EST
GS FLX (Roche)
GAIIx (Illumina)
33.8 Mb
2
280
10,500
EST
16,097
EST
Genomics approach for exploring mechanisms of fruiting body development using Grifola frondosa as model
mushroom
Atsushi Kurahashi1), Masayuki Sato1)2)3), Kozo Nishibori1), Fumihiro Fujimori2)3)
( 1)Yukiguni Maitake Co. Ltd., 2)Hyphagenesis Inc., 3)Tokyo Kasei Univ. )
O-26
Myelochroa
aurulenta
M. aurulenta
Genome Analyzer
37.5 Mbp GC
Augustus
illumina
47.5%
Blast2GO
2
Aspergillus spp.
GC
10,722
3
80
PKS
Genome sequencing and analysis of lichenized fungus
Kojiro Hara, Hikari Sato, Masashi Komine, Yoshikazu Yamamoto
38
(Akita Pref. Univ.)
P-1
Aspergillus awamori
1
1
,
,
ligD
2
1
,
1
,2
Aspergillus awamori
2008
A.awamori NBRC4314
ligD
[
] A.awamori NBRC4314
A.oryzae AoligD
AaligD
pksP
pksP
100%
5’
3’
500bp
pksP
Development of an efficient gene-targeting system in Aspergillus awamori by deletion of the non-homologous end
joining system
Toru Takahashi1, Osamu Mizutani1, Yohei Shiraishi2, Osamu Yamada1
(1NRIB, 2Bio'c Co. Ltd)
P-2
Cre-loxP
*
*
Aspergillus oryzae
Cre-loxP
Cre-loxP
loxP
Cre
Cre
Cre
Cre
loxP
(1)
(1)
loxP
loxP
2009
p. 271
Development of multiple gene expression system using Cre-loxP-mediated marker recycling in Aspergillus oryzae.
Naoki Ebara, Osamu Mizutani*, Takahiro Shintani, Katuya Gomi
(Grad. Sch. Agric. Sci., Tohoku Univ., *NRIB)
39
P-3
1
1
P1
2
UV
Cs#180
1
2
ATCC36910
5-FOA
CsURA5(
Cs#180
PEG
GPD
P1
5-FOA
CsURA5
(CsGPD)
CsURA5
CsURA5(pGEU5)
pGEU5
EGFP
pGPD-EGFP-
Cs#180
1) Yamagishi, K., Kimura, T., Watanabe, T. (2011) Biores. Technol., 102, 6937-6943
Transformation by complementation of a uracil auxotroph of the rice straw degrading basidiomycete Cyathus
stercoreus (bird's nest fungi)
1
Kenji Yamagishi, 1Toshiyuki Kimura, 2Takashi Watanabe (1National Agricultural Research Center, 2Kyoto Univ.)
P-4
Coleophoma empetri F-11899
Coleophoma empetri F-11899
micafungin
FR901379
FR901379
2
genome sequencer
454 FLX FLX; Roche
genome analyzer II GAII; Illumina
C. empetri F-11899
FLX;
469 Mb, GAII;
2,061 Mb
de novo
(
) FLX
GAII
FLX
(
)
FLX
400 b
GAII
75 b
Mb
de novo
FLX
GAII
75 b
FLX
GAII
FLX
GAII
GenomeMacher
GAII FLX
Comparison of genome sequence assembly by two NGSs in fungus Coleophoma empetri F-11899.
Kazuya Nakaya, Masato Yamada, Yohsuke Orino, Yasuhiro Isogai, Seiji Hashimoto
(Dept. of Biotechnol., Toyama Pref. Univ.)
40
P-5
Genome annotation using transcriptome sequence from filamentous fungi
Kiyohiko Igarashi1, Chiaki Hori1, Masahiro Samejima1, Yasuo Uemura2, Aya K. Takeda2
(1Dept. of Biomaterial and Science, Univ. of Tokyo, 2Genaris, Inc.)
P-6
,
,
,
100
Functional genomic analysis of subtropical fungi harboring antibacterial activity
Tomomi Toda, Yoshinori Koyama, Myco Umemura, Hideaki Koike, Masayuki Machida
(Bioproduction RI, AIST)
41
P-7
Aspergillus kawachii IFO 4308
1
1
1
1
1
2
1
2
2
2
1
1
2
Aspergillus kawachii
A. kawachii IFO 4308
Roche 454 GS FLX titanium
36,575,290 bp
11,488
CDS (coding sequence)
(>500 bp)
318
N50
138 kb
1,687
897 kb
CDS
247
enZYmes)
CAZy (Carbohydrate-Active
53
Genome analysis of white Koji mold Aspergillus kawachii IFO 4308
Taiki Futagami1, Kazuki Mori1, Ayaka Yamashita1, Shotaro Wada2, Yasuhiro Kajiwara2, Hideharu Takashita2, Toshiro
Omori2, Kaoru Takegawa1, Kosuke Tashiro1, Satoru Kuhara1, and Masatoshi Goto1
(1 Faculty of Agriculture, Kyushu Univ., 2 Sanwa Shurui, Co. Ltd.)
P-8
SOLiD3
RNA-seq
1
1
2
,
,
1
1
,2
Aspergillus oryzae
2005
RNAseq
30
A. oryzae RIB40
24
SOLiD 3
3.38Gbp
79.7%
CFGD
(URL: http://nribf21.nrib.go.jp/CFGD/)
2x107
Isogen
RNA
Qiagen
110,835,569
70.4%
176,694
113.8
RNA-seq
CFGD
RIB40
RNeasey
RIB40
29.6Mbp
DNAchip
RNA-seq analysis of Aspergillus oryzae and update of Comparative Fungal Genome Database
Kazuhiro Iwashita1, Akiko Shimahara1, Yasuo Uemura2, Osamu Yamada1 (
42
NRIB, 2 Genaris, Inc.)
P-9
VHH
1
1
1
Aspergillus oryzae
2
1
2
2
3
3
1
3
VHH
VHH
EGFR
A
sodM
EGFR-VHH
EGFR-VHH
30
150 rpm
10
EGFR
EGFR-VHH
(sTAA)
GPY
ELISA
A431
80 mg/L
VHH
EGFRVHH
EGFR
CD
EGFR
VHH
A431
Production of camel variable heavy chain antibody fragment VHH by Aspergillus oryzae
Jun-ichi AOKI1, Soichiro TABUCHI1, Fumiyoshi OKAZAKI2, Chiaki OGINO1, Tsutomu TANAKA2, Hiromoto
HISADA3, Yoji HATA3, Akihiko KONDO1 (1Dept. Chem. Sci. Eng., Grad. Sch. Eng., Kobe Univ., 2Org. Adv. Sci.
Tech., Kobe Univ., 3Res. Inst., Gekkeikan Sake Co.)
P-10
A. oryzae
1
2
1
1
1
2
A. oryzae
S-S
,
A. oryzae
Aoatg1
Aoatg4
Aoatg8
Aoatg13
Aoatg15
Aoatg15
5
4
3
thiA
1
Kimura et al. (2011) Biochem. Biophys. Res. Commun., 406: 464-470
Highly efficient production of heterologous proteins by control of autophagy in Aspergillus oryzae
Takashi Kikuma1, Jaewoo Yoon2, Jun-ichi Maruyama1, Katsuhiko Kitamoto1
(1 Dept. of Biotechnol., Univ. of Tokyo, 2 College of Pharmacy, Keimyung Univ.)
43
P-11
A. oryzae
1
1
1
1
1
2
1
1
1
2
3
3
AoxlnR
AoxlnR
1
LC-MS/MS
CAZy database
A. oryzae
OSI1013
LC-MS/MS
4
4
8
SDS-PAGE
NEDO
Expression of xylanase genes from Aspergillus oryzae.
Hiromoto Hisada1, Etsuko Habe1, Kazunori Shiota1, Hiroki Bando1, Hiroko Tsutsumi1, Hiroki Ishida1, Yoji Hata1,
Akihiko Kondo2, Mitsuyoshi Ueda3 (1Res. Inst., Gekkeikan Sake Co., 2Dept. Chem. Sci. Eng., Kobe Univ., 3Div. Appl.
Life Sci., Kyoto Univ.)
P-12
Aspergillus niger
oxaloacetate hydrolase
(oahA)
Aspergillus niger
A. niger WU-2223L
A. niger
oxaloacetate hydrolase(OAH)
A. niger WU-2223L
1,232bp
341
oahA
DOAH-1
oahA
oahA
oahA
EOAH-1
30 g/l
A. niger WU-2223L
oahA
oahA
WU-2223L
OAH
(EOAH-1)
WU-2223L
OAH
12
oahA
oahA
oahA
2
oahA
(DOAH-1) oahA
-PEG
P-No8142
-PEG
35
28.9 g/l(
96%)
OAH
Disruption and overexpression of oxaloacetate hydrolase gene (oahA) in citric acid-producing Aspergillus niger
Keiichi Kobayashi, Yuki Honda, Takasumi Hattori, Kohtaro Kirimura
(Dept. Appl. Chem., Fac. Sci. Eng., Waseda Univ.)
44
P-13
[
]
,
21
570
,
50
,
,
,
,
,
,
,
[
,
]
,
,
AC+Wash
,
AC+Wash
,AC+Wash
1
,
,
,
27
,
,DNA
3
Study on solubilization by the Aspergillus oryzae fermentation using the starch removal wheat sliding paper-door
Akira Suzuki1, Masato Wada1, Tsukasa Nakamura1, Motoaki Sano1, Kenji Ozeki1, Siniti ohashi1,akihiro kaneko2
(1KIT, 2nissin pharma)
P-14
Analysis of 25kDa protein gene on Aspergillus oryzae liquid cluture
Hiroaki Ishizaki, Taisuke Awakura, Momoko Tedoriya, Motoaki Sano, Kenji Ozeki, Shiniti Ohashi
(KIT, Univ. GENOMU Labo.)
45
P-15 (O-24)
1,2
1,2
2
2
2
2
1, 2
1
2
55
9
55
40%
A40Y
(A40Y)
60%
(S60Y)
S60Y
Evolution of industrial Aspergillus oryzae genome and its adaptation to the fermentation industries
Gaku Ito1, 2, Yuhei Senoo1, 2, Tuneko Sawamura2, Masahumi Tokuoka2, Atsuko Isogai2, Osamu Yamada2, Kazuhiro
Iwashita1, 2
(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB)
P-16
50
301
Uniplot
257
BLAST
156
147
130
Analysis of Aspergillus oryzae genes conserved among filamentous fungi but not characterized the functions.
Humiko Tomikawa 2, Nao Imaru 1. Shiho Terado 1. Yuriko Ikeda 2. Masatoshi Goto3. Kazuhiro Iwashita 1,2
1
AdSM,Hiroshima Univ
2
NRIB
3
Kyushu Univ
46
P-17
1,2
1,2
(1
2
2
2
1,2
2
)
160
(
1
)
55
2
3
6
1
B
15
(glaB)
-
20
3
2
3
1%
3
Functional characterization of RKP (Rice koji protein) disruptants in sake brewing procedure
Teruaki Hanada1.2, Shinichiro Fukuhara1.2, Junko Shitamoto.2, Minori Kono.2, Kazuhiro Iwashita1.2, Osamu Yamada.2
(1 Grad. Sch. Adv. Sci. Mat., Hiroshima Univ., 2 NRIB )
P-18
porphobilinogen deaminase
,
,
,
Aspergillus nidulans
nitrosative stress
,
(RNS)
RNS
RNS
A. nidulans
AN0121
WT
AN0121
FHb
Nir
PBG-D
RNS
RNS
RNS
porphobilinogen deaminase (PBG-D)
PBG-D
PBG-D
ALC
PD1>WT>ALC
RNS
AN0121
PD1
WT
RNS
PBG-D
Flavohemoglobin FHb
nitrite reductase (Nir) RNS
WT, PD1, ALC
FHb
Nir
PBG-D
RNS
FHb
Nir
RNS
Heme-biosynthetic porphobilinogen deaminase protects Aspergillus nidulans from nitrosative stress.
Shengmin Zhou, Toshiaki Narukami, Misuzu Nameki, Yosuke Kamimura, Takayuki Hoshino , Naoki Takaya
(Graduate School of Life and Environmental Sciences, Univ. of Tsukuba)
47
P-19
AoSO
, Cristopher Sarazar ESCAÑO,
,
SO/Pro40
Aspergillus oryzae
AoSO
1)
AoSO
AoSO
AoSO
AoSO
AoSO
EGFP
1195
AoSO
Saccharomyces cerevisiae
S. cerevisiae
AoSO-EGFP
AoSO
556-1146
A. oryzae
AoSO[556-1146]
AoSO
EGFP
AoSO
1) J. Maruyama et al. (2010) Biochem. Biophys. Res. Commun. Vol. 391, 868-873
Deletion analysis of A. oryzae AoSO protein for its aggregation mechanism in response to stresses
Kei SAEKI, Cristopher Sarazar ESCAÑO, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO
(Dept. of Biotechnol., Univ. of Tokyo)
P-20
Aspergillus nidulans
ypdA
His-Asp
Aspergillus nidulans
YpdA
NikA
YpdA
Conditional-ypdA
alcA
YpdA
CypdA
SskA
SrrA
ypdA
YpdA
CypdA
FGSC A89
ypdA
YpdA
ABPU1
CypdA
CypdA
alcA
ypdA
ypdA
sskA
HOG
srrA
YpdA
Analysis of the conditional-ypdA strain in Aspergillus nidulans
Yura Midorikawa1, Daisuke Hagiwara2,3, Akira Yoshimi 2, Keietsu Abe1,2
(1 Grad. Sch Agric. Sci., Tohoku Univ., 2 Tohoku Univ. NICHe , 3 Present address: MMRC, Univ. of Chiba)
48
P-21
Aspergillus nidulans
PhkA,PhkB
A. nidulans
15
HK
RR
Schizosaccharomyces pombe
HK
15
phk1/mak2 , phk2/mak3
HPt ,4
HK
phkA , phkB
phk3/mak1
3
S. pombe
A. nidulans
PhkA,PhkB
phkA
phkB
2
phkA
1.5mM
phkB
2mM
phkB
PCR
1)
NADPH
Nakamichi,N.et al. (2002) Biosci.Biotechnol.Biochem.66(12):pp.2663-2672
Characterization of Histidine kinase PhkA,PhkB in Aspergillus nidulans
Tomomi Sako,Yukari Yamazaki,Kyoko Kanamaru,Masashi Kato1,Tetsuo Kobayashi
(Grad. Sch. of Bioagricultual Sciences, Nagoya Univ. 1Sch .of Agriculture , Meijo Univ)
P-22 (O-1)
A. oryzae
MAP
AoFus3
Özgür Bayram
1
Oliver Valerius1
Gerhard H. Braus1
1
MAP
Saccharomyces cerevisiae
MAP
Fus3
Fus3p
A. oryzae
Fus3p
Aofus3
AoFus3
AoFus3
Fus3p
A. oryzae
AoFus3
AoFus3
C
TAP(Tandem Affinity Purification)
Aofus3
LC/MS/MS
AoFus3
2
RACE
EGFP
2
AoFus3
Identification and functional analysis of novel AoFus3(MAP kinase)-interacting proteins in Aspergillus oryzae
Daiki YAHAGI, Jun-ichi MARUYAMA, Özgür BAYRAM1, Oliver VALERIUS1, Gerhard H. BRAUS1, Katsuhiko
KITAMOTO (Dept. of Biotechnol., Univ. of Tokyo,1Georg-August-Universität Göttingen)
49
P-23
Aspergillus fumigatus
Aspergillus fumigatus
HogA/SakA MAPK
HAMP
N
NikA
HogA/SakA MAPK
A. fumigatus
NikA
SakA MAPK
NikA
SakA
NikA
SakA
catA
A. nidulans
SakA
Functional analysis of HOG pathway in opportunistic pathogen Aspergillus fumigatus
Daisuke Hagiwara, Tohru Gonoi, Susumu Kawamoto
(MMRC, Chiba Univ.)
P-24
Neurospora crassa
,
MAK-1
,
MAK-2 MAP
,
,
Slt2p MAP
, Neurospora crassa
MAK-1 (
,
Fus3p
)
, Δmak-1
Congo Red
Δmak-2
beta-1,3-glucan
N. crassa
2-sgfp
,
MAK-1
2
CWI
Calcofluor White
, micafungin
SDS
Δmak-1
,
MAP
GFP
mak-1-sgfp
, MAK-1
, MAK-1
MAK-2
Δmak-1
Δmak-2
,
,
polyoxin
MAK-2
, MAK-1
,
micafungin
MAP
(cell wall integrity:CWI)
Slt2p
)
, MAK-2 (
Pheromone
nikkomycin
MAK-2
Localization of two MAP kinases, MAK-1 and MAK-2 in Neurospora crassa.
Masayuki Kamei, Masakazu Takahashi, Akihiko Ichiishi and Makoto Fujimura
(Grad. Sch. of Life Sci. , Toyo Univ.)
50
, mak,
P-25 (O-3)
Aspergillus nidulans
C
,
C PKC
PKC
pkcA
42
,
2)
pkcA
ROS
Aspergillus nidulans
DNA
1)
pkcA
30
, 42
pkcA
,
DNA
,
42
,
, DNA
,
PkcA
PkcA
MAP
,
MAP
bckA, mpkA
42
, DNA
,
, ROS
,
,
BckA, MpkA
42
PkcA
MAP
1) Ichinomiya, M., et al., (2007) Biosci. Biotechnol. Biochem., 71, 2787-2799
2)
,
2010
, p213
Analysis of PkcA function in the suppression of apoptosis under heat stress condition in Aspergillus nidulans
Takuya Katayama, Hiroyuki Horiuchi and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)
P-26
Aspergillus oryzae
AoApsB
Aspergillus nidulans
organizing center)
ApsB
A. oryzae
ApsB
MAP
(microtubuleAoFus3
MAP
A. oryzae
A. nidulans apsB
AO090020000040
A. oryzae
AoapsB
BLAST
AoApsB
AoapsB
AoapsB
AoApsB
Aofus3
AoFus3
Functional characterization of microtubule-organizing center component AoApsB in Aspergillus oryzae
Junpei KAWABATA, Daiki YAHAGI, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO
(Dept. of Biotechnol., Univ. of Tokyo)
51
P-27
A. oryzae
A2
AoPlaA
A2 (PLA2)
PLA2 (cytosolic PLA2; cPLA2)
cPLA2
cPLA2
AoPlaA
AoPlaA
AoPlaA
AoplaA
MS/MS
AoplaA
(PE)
34:1
36:3
34:2
36:4
PE
AoplaA
AoplaA
15°C
PD
AoPlaA
Functional analysis of cytosolic phospholipase A2-like protein AoPlaA in A. oryzae
Noriyuki Komai, Shohei Kotani, Katsuhiko Kitamoto, Manabu Arioka
P-28
A. oryzae
Ca2+
A2
A2 (PLA2)
PLA2
cytosolic PLA2 (cPLA2)
iPLA2
Ca2+
A. oryzae
(AoPlaA)
A. oryzae
300 bp
2
cDNA
iplaA
sPLA2 (sPlaA sPlaB)
cPLA2
A. oryzae iPlaA (AO090011000913)
DOGAN
ORF
DOGAN
2
iPlaA-EGFP
90 kDa
AIE
iPlaA
sn-2
secretory PLA2 (sPLA2)
2+
Ca -independent PLA2 (iPLA2)
3
iPLA2
DPY
PCR
(Dept. of Biotechnol., Univ. of Tokyo)
RACE
200 bp
601
AIE
EGFP
iPlaA
iPlaA
Identification and characterization of putative Ca2+-independent phospholipase A2-like protein iPlaA in A. oryzae
Sou Kohashiguchi, Katsuhiko Kitamoto, Manabu Arioka
(Dept. of Biotechnol., Univ. of Tokyo)
52
P-29
A. oryzae
aipC, aipD
yeast two-hybrid
AipC
AoAbp1
Aspergillus
S. cerevisiae
Aspergillus
bait
1)
AipD
SH3
S. cerevisiae
2
aipC
AipC
1
AoAbp1
1)
AoAbp1
4
aipA D (AoAbp1 interacting protein)
App1p (actin patch protein)
A. oryzae
aipD
RACE
798
AipD
399
aipC
yeast two-hybrid
9
AipD
cDNA
aipD
cDNA
p. 31
Analysis of aipC and aipD related to endocytosis in Aspergillus oryzae
Kento MATSUO, Yujiro HIGUCHI, Manabu ARIOKA, Katsuhiko KITAMOTO
(Dept. of Biotechnol., The Univ. of Tokyo)
P-30
(
)
(MalP)
1)
MalP
MalP
GFP-MalP
GFP-MalP
GFP
GFP
6-
2-
3-
-O-
MalP
MalP
1) Hasegawa et al. Fungal Genet. Biol. 47, 1-9 (2010).
Intracellular localization of maltose permease (MalP) in response to carbon sources in Aspergillus oryzae
Tetsuya Hiramoto
Ryoko Daidouguchi
Mizuki Tanaka
Takahiro Shintani
(Div.Biosci.Biotechnol.Future Bioind.,Grad.Sch.Agric.Sci.,Tohoku Univ.)
53
Katsuya Gomi
P-31 (O-4)
Aspergillus nidulans
agsA, agsB
1
2
,
-1,3-
1,2
,
agsB
1
,2
, Aspergillus nidulans
, MAP
,
AGS
AGS
MpkA
MpkA
-1,3,
AGS
,
, agsB
,
,
agsA, agsB
, agsA
, agsB
, agsA
AgsB
1,3AGS
agsB
agsB
2
-
, agsB
, Congo Red
,
, agsB
agsB
, agsA
agsB
,
, NMR
-1,3-
-1,3-
, Aspergillus
AGS
,
Analysis of the disruptant of
-1,3-glucan synthase gene agsB and the double disruptant of agsA and agsB in
Aspergillus nidulans
Azusa Inaba 1, Akira Yoshimi 2, Keietsu Abe 1,2
( 1 Grad. Sch. Agric. Sci., 2 Tohoku, Univ., NICHe.,Tohoku Univ.)
P-32
Aspergillus nidulans
A. nidulans
CsmA
V
csmA
CsmA
N
KinA
1)
CsmA
CsmA
KinA
KipA
CsmA
kipA
EGFP-CsmA
CsmA
EGFP-
kinA
EGFP-CsmA
rigor
EGFP-KipA
mDsRed-CsmA
EGFP-KipArigor
CsmA-HA
CsmA
1)
EGFP-KipA
rigor
KinA
KipA
p. 198
2010
The roles of kinesins in the localization of chitin synthase CsmA in Aspergillus nidulans
Makusu Tsuizaki, Hiroyuki Horiuchi, and Akinori Ohta. (Dept. of Biotechnol., Univ. of Tokyo)
54
mDsRed-CsmA
HA
P-33
Aspergillus nidulans
Saccharomyces cerevisiae CRH
12%
10-30%
S. cerevisiae
nidulans
5
Crh (Crh1p Crh2p)
(crhA crhB crhC crhD
CRH
A. nidulans A1149(ΔnkuA)
ΔcrhA
A.
crhE)
5
CRH
ΔcrhB ΔcrhC ΔcrhE
ΔcrhD
76%
A. nidulans
crh
Functional analyses of CRH orthologs of Saccharomyces cerevisiae in Aspergillus nidulans.
Koji Isomura, Makusu Tsuizaki, Hiroyuki Horiuchi, and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)
P-34
Aspergillus nidulans
AN3445
Saccharomyce cerevisiae
Saccharomyces cerevisiae SKT5
division 2
Chs3p
Chs3p
Chs3p
division 2
Skt5p
CsmA
A. nidulans
CsmB
SKT5
AN3445
EGFP-AN3445
AN3445
division 2
EGFP-CsmA
CsmB
1)
AN3445
CsmA
1)
Chs3p
bud neck
Aspergillus nidulans
2010
CsmA-FLAG
AN3445
AN3445
3
CsmA
EGFP-AN3445
CsmA
p. 212
Functional analysis of a Saccharomyces cerevisiae SKT5 ortholog in Aspergillus nidulans
Hiroomi Hoshi, Makusu Tsuizaki, Hiroyuki Horiuchi, and Akinori Ohta (Dept. of Biotechnol., Univ. of Tokyo)
55
P-35
A. oryzae
Aoatg11
/
A. oryzae
S. cerevisiae
Atg11p
A. oryzae
AoAtg11
Aoatg11
A. oryzae
AoAtg8
EGFP
AoAtg11
Aoatg11
AoApe1
AoAtg11
Analysis of Aoatg11 related to selective autophagy in Aspergillus oryzae
Takayuki Tadokoro, Takashi Kikuma, Katsuhiko Kitamoto (Dept. of Biotechnol., Univ. of Tokyo)
P-36
Magnaporthe oryzae
1) 3)
1)
1)
2
2) 3)
1) 3)
1) 3)
3)
Magnaporthe oryzae
12
24
Woronin
Differentiation in Magnaporthe oryzae regulated metabolism in conidia via autophagy
Kanako Inoue1) 3), Satoko Kanematsu2) 3), Pyoyun Park2) 3), Kenichi Ikeda2) 3)
(Kobe Univ.1), Natl. Inst. Fruit Tree Sci.2), Bio-oriented Tech. Res. Adv. Inst.3) )
56
P-37
CcAtg8
Coprinopsis cinerea
CcAtg8
CcAtg8
EDTA
CcAtg8
Ccatg8
dsRNA
RNAi
Analysis of CcAtg8 involved in autophagy of Coprinopsis cinerea
Akira Watanabe, Takahiro Yayoi, Masanori Yoshimura, Yasuhiko Asada
(Dept. of Appl. Biol. Sci., Fac. of Agr., Univ. of Kagawa)
P-38
Coprinopsis cinerea
1
2
,
2
,
1
1
,2
Coprinopsis cinerea
C. cinerea
C.
cinerea
Involvement of myosins in protein secretion by basidiomycete, Coprinopsis cinerea
Kohsuke Hashimoto1, Etsuo Yokota2, Teruo Shimmen2, Makoto Yoshda1
(1Fac. of Agri., Tokyo Univ. of Agri. & Tech., 2Grad. Sch., Life Sci., Univ. Hyogo)
57
P-39
cag1
F1
2.89, P>0.05
growthless1 (cag1)
47
65
cap-
cag1
F1
DNA
RAPD PCR
IX
G13-900B
cag1
BAC DNA
trp1
+
RAPD
15%
#58 (cag1-1 trp1-1, 1-6)
BAC
G13-900B
7H8
1F2
(cag1+)
7H8
DNA
HindIII
B4
B4
HindIII
B4
DNA
HindIII
cag1
Analysis of the cag1 gene, which is involved in cap growth of Coprinopsis cinerea
Hajime Muraguchi, Kazuki Kemuriyama
(Dept. of Biotechnology, Akita Prefectural Univ.)
P-40 (O-6)
Epichloë festucae
G
Cdc42
RacA
E. festucae
E. festucae
NoxA
G
RacA
NoxR
NoxA
BemA/Cdc24
RacA
NoxR
RacA
Cdc42
Nox
RacA
NoxR
Cdc42
Yeast two hybrid
BemA
racA
N
cdc42
racA
cdc42
cdc42
Cdc42
Cdc42
RacA
Functional analysis of small G proteins Cdc42 and RacA from fungal endophyte Epichloë festucae
Yuka Kayano, Daigo Takemoto
(Grad. School Bioagr. Sci., Nagoya Univ.)
58
P-41
1
1
4
1
1
1
1
1
2
3
2
2
3
4
Aspergillus oryzae KBN8048
503
KBN8048
pH
13
type R
type S
type R
5.0
RT-PCR
type S
pH
Transcriptional profiles of the acid phosphatase genes in A. oryzae low acid phosphatase activity strain in solidstate rice and soybean cultures for miso brewing
Junichiro Marui1, Sawaki Tada1, Mari Fukuoka1, Satoshi Suzuki1, Ryota Hattori1, Yutaka Wagu2, Yohei Shiraishi2,
Noriyuki Kitamoto3, Tatsuya Sugimoto, Ken-Ich Kusumoto1
(1NFRI, 2Bio’c, 3Food Res. Center, Aichi Ind. Tech. Inst., 4Nakamo)
P-42
A. oryzae KBN630
(Aph)
1
3
1
1
2
1
2
3
Aph
Aph
aph
aph
Aph
A. oryzae KBN630
oryzae KBN630-17K3
niger phyA
ku70
pyrG
90
8
aphA
aphH
3
A. niger phoA
2
aphI
A.
A.
A. niger aphA
aphM
13
aphC
Disruption of the acid phosphatase (Aph) genes in the miso koji mold, A. oryzae KBN630
Shoko YOSHINO-YASUDA, Osamu HASEGAWA, Natsuko ONO, Yoshimi IGA1, Yohei SHIRAISHI1, Yutaka
WAGU1, Tatsuya SUGIMOTO2, Ken-Ichi KUSUMOTO3, Noriyuki KITAMOTO ( Food Res. Center, Aichi Ind. Tech.
Inst., 1 Bio’c CO.,LTD, 2 NAKAMO CO.,LTD, 3 Nat’l Food Res. Inst. )
59
P-43 (O-19)
Aspergillus oryzae
Hydrophobin
Hydrophobin
70~150a.a.
Hydrophobin
Aspergillus oryzae
hypA,B,C,D
hypA,B,C
HypA
HypB,C
hypA, B, C
Hydrophobin
4
HypA,B,C
Hydrophobin
HypA
PCR
SEM
A. oryzae
HypD
Hydrophobin
Functional analysis of Hydrophobins by multiple gene disruption in Aspergilllus oryzae
Yui Yamakawa, Yuka mizuno, Harushi Nakajima
(Dept. of Agricultural Chemistry, Univ. of Meiji)
P-44 (O-20)
(QCM)
PBSA
RolA
CutL1
hydrophobin
PBSA
PBSA
RolA
PBSA
Polybutylene succinate-co-adipate
cutinase CutL1
hydrophobin RolA
PBSA
CutL1
pH
RolA
RolA
RolA
PBSA
RolA
PBSA
PBSA
L137S, L142S, D134A/D136A,
RolA
RolA
RolA
K130A/D134A/D136A
PBSA
PBSA
PBSA
QCM
RolA
1) Takahashi et al., Mol Microbiol. 57: 1780-1798 (2005)
QCM analysis of the interaction between Aspergillus oryzae hydrophobin RolA and solid surfaces
Hiroki Tanabe1, Keiko Orui 1, Kenji Uehara1, Toru Takahashi2, 3, Takanari Togashi4, Toshihiko Arita4, Keietsu Abe1, 2
(1 Grad. Sch. Agric. Sci., Tohoku Univ., 2 NICHe., Tohoku Univ., 3 NRIB., 4 IMRAM., Tohoku Univ.)
60
P-45
hydrophobin
1
1
1
Fv-hyd3
2
1
1
2
Hydrophobin
hydrophobin
Hydrophobin
2
hydrophobin
Fv-hyd1
Fv-hyd3
RT-PCR
2
1
hydrophobin
cDNA
pGEX 5X-
E.coli Rosetta2(DE3)
Fv-hyd3
SDS-PAGE
37k Da
GST
Fv-hyd3
Fv-hyd3
Expression of hydrophobin (Fv-hyd3) fusion protein derived from Flammulina velutipes
Masumi Yoshida1, Masayuki Fujita1, Ryohei Nishikawa1, Satoshi Inatomi2, Goro Taguchi1, Makoto Shimosaka1
( 1Division Appl.Bio., Fac.Tex.Sci.Shinshu Univ., 2Mushroom Lab.Hokuto Co. )
P-46
KexA
serine-type carboxypeptidase
Saccharomyces cervisiae
KexA
Lys,Arg
12
KEX1
S.cerevisiae
Kex1p
exo
KexA
KexA
Calcofluor White
KexA
Functional analysis of KexA in Aspergillus oryzae
Sayako Tomita, Hiroto Morita, Hirosi Maeda, Mitio Takeuti, Youhei Yamagata
(Dept. of Applied Biological Science, Tokyo univ. of Agriculture and Technology)
61
C
P-47
serine-type carboxypeptidase
Aspergillus nidulans
Aspergillus oryzae
12
12
CPase
serine-type carboxypeptidases (CPase)
Aspergillus nidulans
5
CPase
CPase
TA4
TA13
5
CPase
A.
nidulans A89
PCR
5
5
TA13
5
CPase
SDS-PAGE
CPase
Serine-type carboxypeptidase from Aspergillus oryzae hyper expression mutants in Aspergillus nidulans
Kazunori Sano, Hiroto Morrita, Hiroshi Maeda, Yohei Yamagata, Michio Takeuchi
(Dept. of Applied Biological Science, Tokyo Univ. of Agriculture and Technology)
P-48
β
β
β
(1)
BglA
GH family 3
β
p-nitrophenyl-β-D-glucopyranoside (pNPG)
β
BglA
pNPG
β
BGL1
BglA
(2)
pNPG
BglA
(1)
p. 31
(2)Kaya. et al., Appl Microbiol Biotechnol (2008) 79:51-60
The Unique Substrate Specificity of Novel β-Glucosidases from Aspergillus oryzae
Kanako Kudo, Seiryu Ujiie, Akira Watanabe, Takahiro Sintani, Katsuya Gomi
(Div. Biosci. Biotechnol. Future Bioind., Grad. Sch. Agric. Sci., Tohoku Univ.)
62
BGL1
P-49
1
1
,
1
2
,
1
,
1
2
,
3
,
2
I
,
4
II
CBH I, CBH II
LBKP
BGL
CBH I
3
,
3
EG
EG
4
,
BGL
LBKP
LBKP
Consolidated BioProcessing
CBP
CBP
Enzymatic saccharification of kraft pulp using cellulase mixture produced by recombinant Aspergillus oryzae.
Toshihide YOSHIE1, Shoji SAKAI1, Fumiyoshi OKAZAKI2, Chiaki OGINO1, Tsutomu TANAKA2, Hiromoto
HISADA3, Shin-ya HAGIWARA4, Yoji HATA3, Akihiko KONDO1 (1Dept. Chem. Sci. Eng., Grad. Sch. Eng., Kobe
Univ., 2Org. Adv. Sci. Tech., Kobe Univ., 3Res. Inst., Gekkeikan Sake Co. , 4JPRI)
P-50
Phanerochaete chrysosporium
櫻木 潔 , 堀 千明 , 林 礼子 , 三橋 秀一 , 五十嵐 圭日子 , 鮫島 正浩
1
1
2
2
1
1
（ 東大院農生科、 バイオエタ
1
2
ノール革新技術研究組合）
セルロース系バイオマスの酵素糖化は, アルコールやプラスチックの原料となる単糖を穏和な条件で得る
ために重要な技術である。酵素糖化を効率的に行うためには前処理が必要であり, その一つにアンモニア処
理がある。これまでの研究によって, アンモニア処理はシラカバやヤナギなどの広葉樹材に対して効果が高
いことを見いだした。しかし, バイオマスのどのような変化が酵素糖化効率を上げているかに関しては依然
不明な点が多い。そこで本研究では, シラカバの未処理木粉とアンモニア処理木粉を基質として担子菌
Phanerochaete chrysosporiumを培養し, 得られた菌体外液に含まれる主要なタンパク質のセクレトーム解析を
行うことで, アンモニア処理が本菌による菌体外酵素の生産に与える影響について調べた。その結果, アン
モニア処理によってエステラーゼやキシラナーゼ, キシログルカナーゼの分泌パターンに変化が起こってい
ることが明らかになった。このことは, 本菌がアンモニア処理によるヘミセルロース構造の変化に対応して
酵素を生産している可能性を示しており, セルロース系バイオマスを糖化する際の酵素選択に重要な情報を
与えると考えられた。
Secretome analysis on extracellular proteins of basidiomycete Phanerochaete chrysosporium grown on ammonia
treated wood
Kiyoshi Sakuragi1, Chiaki Hori1, Reiko Hayashi2, Shuichi Mihashi2, Kiyohiko Igarashi1, Masahiro Samejima1.
(1Dept. Biomat. Sci., Univ. of Tokyo., 2RAIB)
63
P-51
DNA
ITS
DNA
Phi29 DNA
PCR
ITS
ITS
ITS
GAPDH
2
3
GAPDH
Phylogenetic classification of wood rotting fungi using enzyme genes related to carbohydrate metabolism
Tomoko Wada, Kiyohiko Igarashi, Masahiro Samejima
P-52 (O-8)
A. oryzae
SclR
1
,
(Dept. of Biomaterial and Science, Univ. of Tokyo)
1
,
,
,
A. oryzae
,1
(
)
,
,
,
,A. oryzae
(sclerotium)
,
,
,
,
(perithecium)
,
A. oryzae
,
,
,
/
sC
(ΔpyrG)
(ΔadeB)
,
,
,
,
,
,A.
oryzae
,
SclR
,
Analysis of hyphal fusion ability by using the strains overexpressing SclR, a promoting factor for sclerotia
formation in Aspergillus oryzae
Ryuta WADA, 1Feng Jie JIN, 1Yasuji KOYAMA, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO
(Dept. of Biotechnol., Univ. of Tokyo, 1Noda Ins. Sci. Res.)
64
P-53
Aspergillus nidulans
NmrA
,
AreB
,
A. nidulans
NmrA
NAD(P)+
NAD(P)H
NAD(P)+/NAD(P)H
NmrA
glucose-6-phosphate
6-phosphogluconate
glycerol
A. nidulans
(MD)
NmrA
GATA
AreB
Complementation
AreA
MD
NmrA
Bimolecular Fluorescence
AreB
GST pull-down
AreB
in vitro
NADP
NmrA
+
NADPH
AreB
NmrA
MD
NADP
+
AreB
NADPH
The Aspergillus nidulans NmrA and AreB are involved in menadione stress tolerance.
Eriko Ito, Shunnsuke Masuo, Motoyuki Shimizu, Naoki Takaya
(Graduate Sch. of Life and Environmental Sci., Univ. of Tsukuba)
P-54
Aspergillus oryzae
DNA
PCR
AO090005000179 (hypB)
ΔHYP
ΔHYP
ΔHYP
pH
pH
ΔHYP
ΔHYP
hypB
pH
hypB
pH
A. oryzae
HYP
HYP
HYP
hypB
Transcription factor regulating growth and development of Aspergillus oryzae under stress conditions
Akinori Kuroda
Naoki Takaya
(Graduate School of Life and Environmental Sciences
Univ. of Tsukuba)
65
brlA
P-55
Epichloë festucae
1
Sanjay Saikia2
2
Massey Univ.
Epichloë
festucae
EfProA
Gemma Cartwright2
ProA
1
3
1
1
3
E.
Zn(II)2Cys6
ProA/NosA
EfPROA
EfPROA
esdC
ProA
Barry Scott2
Aspergillus nidulans
EfESDC
ProA
EfESDC
GCGCCG
EfESDC
EfESDC
EF320
EfESDC
EfESDC
EfPROA
EfESDC
EF320
EF320
ProA
EfESDC
ProA
GCGCCG
2.4 kb
1
EF320
Identification of the promoter sequence for the ProA binding in Epichloë festucae, a mutualistic symbiont of
perennial ryegrass.
Aiko Tanaka1, Sanjay Saikia2
Scott2
Gemma Cartwright2
(1Grad. Sch. Bioagric. Sci., Nagoya Univ.
Daigo Takemoto1
Masashi Kato3
2
IMBS, Massey Univ.
Takashi Tsuge1
Barry
3
Dept. of Agr., Meijo Univ.)
P-56
Aspergillus nidulans AmyR
,
,
Aspergillus
,
AmyR
isomaltose (IM)
AmyR
, IM
AmyR
IM
AmyR
GFP-AmyR
AmyR
GFP-AmyRH478L
GFP-AmyR
IM
GFP-AmyRpmw
IM
SV40
GFP-AmyR
IM
NLS
IM
GFP-AmyR
IM
IM
GFP-AmyR
AmyR
IM
Analysis on inducer-dependent degradation of AmyR in Aspergillus nidulans
Akio Osaki, Shota Morimoto, Kyoko Kanamaru, Masashi Kato, Tetsuo Kobayashi
(Graduate Sch. of Bioagric. Sci., Nagoya Univ.)
66
NLS-GFP-AmyRpmw
IM
NLS-
P-57
Aspergillus aculeatus ace1
Aspergillus aculeatus
Trichoderma reesei AceI
Aspergillus nidulans StzA
A. aculeatus Ace1
aculeatus ace1
A. aculeatus Ace1
A. aculeatus ace1
endoglucanase, endoxylanase
β-glucosidase(BGL)
2
ace1
100, 300, 500 mM NaCl
YPD
300, 500 mM NaCl
A. aculeatus BGL
Ace1
AceI/StzA
Aspergillus
A. aculeatus Ace1
BGL1
Production of biomass degrading enzymes and salt stress response in Aspergillus aculeatus ace1 deficient mutant
Manami Yamazaki, Hirokazu Konishi, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi
( Grad. Sch. Life & Env. Sci., Osaka Pref. Univ.)
P-58
Aspergillus aculeatus Zn(II)2Cys6
Aspergillus aculeatus
CGAF
cellobiohydrolase I
(cbhI)
carboxymethyl cellulase 2
(cmc2)
XlnR
Zn(II)2Cys6
genes-activting factor (cgaF)
DNA
CGAF
cgaF
PCR
cbhI
cmc2
XlnR
xylanase
cellulase
cmc1
FIb-
(xynIb)
transcription elongation factor 1α
(tef1)
10
cgaF
2
CGAF
Zn(II)2Cys6 type transcription factor CGAF activates expression of the cellulase and hemicellulase genes in
response to cellulosic carbon sources in Aspergillus aculeatus
Emi Kunitake, Shuji Tani, Jun-ichi Sumitani, Takashi Kawaguchi
67
(Grad. Sch. Life Env. Sci., Osaka Pref. Univ.)
5
P-59
Aspergillus oryzae
ManR
A. oryzae
A. nidulans
ManR
ManR
CMC
konjac glucomannan
EglA
EglB
4-5
2
2
EglA
EglB
ManR
ManR
CelR
Regulation of cellulase synthesis in filamentous fungi
Miki Aoyama, Kyoko Kanamaru, Tetsuo Kobayashi
(Graduate Sch. of Bioagric. Sci., Nagoya Univ. )
P-60 (O-10)
XlnR
,
,
,
,
Aspergillus oryzae
,
XlnR
,
,
, XlnR
,XlnR
phosphate affinity SDS-PAGE
,
XlnR
c-myc
XlnR
DNA
phosphate affinity SDS-PAGE
c-myc::XlnR
,
,
XlnR
3-4
2
,
XlnR
EMSA
,XlnR
ChIP Assay
,
XlnR
DNA
XynF1
DNA
,
,
Inducer-dependent phosphorylation of the transcriptional activator XlnR and its physiological role in Aspergillus
oryzae
Shuhei Ishikawa, Yuji Noguchi, Kyoko Kanamaru, Masashi Kato , Tetsuo Kobayashi
(Graduate Sch. of Bioagric. Sci., Nagoya Univ., Sch. Agric. Sci., Meijo Univ.)
68
P-61 (O-11)
Trichoderma reesei
,
,
Trichoderma reesei
T.reesei
9129
Functional analysis of genes encoding a putative cello-oligosaccharide transporter in the filamentous fungus
Trichoderma reesei.
Hideyuki Kusaka, Takanori Furukawa, Eiji Fukaya, Yousuke Shida, Wataru Ogasawara
(Deot. Bioeng., Nagaoka Univ. of Tech)
P-62
Trichoderma reesei
Trichoderma reesei
XYRI
T. reesei
T. reesei
SDS-PAGE
,
XYR1, ACEI, ACEII, CreI
,
,
,
,
Search for novel transcriptional mechanism in Trichoderma reesei
Haruna Sato, Masahiro Yuki, Wataru Ogasawara
(Dept. of Bioeng., Nagaoka Univ. of Tech.)
69
P-63
Trichoderma reesei
1,2
ClbR
1
,
1
,
, 2 JST, 3
3
,
,4
Trichoderma reesei
ORF
4
,
3
,
1
,
1
,
1
DNA
PC-3-7
9
SNPs
KDG-12
Avicel
2
PC-3-7
9
SNP
9
Regulator
1
Blast
AmyR
SNPs
Zn(II)2Cys6
Aspergillus
PC-3-7
DNA
ClbR:Cellobiose responsing
AoAmyR
2
ClbR
ClbR
PC-3-7
clbR
SNP
KDG-12
ORF
QM6a
PC-3-7
KDG-12
clbR
SNP
PC-3-
7
Identification of new Zn(II)2Cys6 type transcription factor involved in cellobiose hydrolysis in Trichoderma
reesei
Mikiko Nitta1,2, Kaori Yamaguchi1, Yosuke Shida1, Kazuki Mori3, Hideki Hirakawa4, Satoru Kuhara3, Yashushi
Morikawa1, Wataru Ogasawara1 (1 Nagaoka Univ. of Tech., 2 JST, 3 Kyushu Univ., 4 Kazusa DNA Inst.)
P-64
AOEXE103
Aspergillus oryzae
(CPase)
1775 bp
A.oryzae
12
AOEXE103
12
3
CPase
1
mRNA
DNA
cDNA
AOEXE103
A.oryzae RIB40
N
mRNA
AOEXE103
cDNA
Czapeck-Dox
PCR
PCR
mRNA
g
cDNA
AOEXE103 cDNA
3
3
AOEXE103
Alternative splicing of protease gene AOEXE103 in Aspergillus oryzae.
Megumi Kuboshima, Hiroto Morita, Hiroshi Maeda, Ayako Okamoto, Youhei Yamagata, Michio Takeuchi
(Tokyo Univ. of Agriculture and Technology)
70
P-65
A.oryzae
12
2
4
12
12
A.oryzae RIB40
mRNA
Czapeck-Dox
cDNA
cDNA
PCR
12
11
mRNA
N
Semiquantitative transcriptional analysis of serine-type carboxypeptidase encoding genes in Aspergillus oryzae
Haruka Abo, Hiroto Morita, Ayako Okamoto, Hiroshi Maeda, Yohei Yamagata, Michio Takeuchi
(Tokyo Univ. of Agriculture and Technology)
P-66
ABC
AtrR
AtrR
ABC
ABC
PDR1/PDR3
GAL11/MED15
GAL11
ABC
TAP
Purification
HSP70
SSZ1
AtrR
Tandem Affinity
AtrR
AtrR
HSP70
PDR1
PDR3
HSP70
HSP70
SSA1
SSA1/SSA2
AtrR
SSA1
Screening for the factor(s) that interacts with the transcription activator AtrR involved in ABC transporter gene
expression
Ayumi Ohba, Mizuki Tanaka, Shintani Takahiro, Katsuya Gomi
(Div.Biosci.Biotechnol.Future Bioind., Grad.Sch.Agric.Sci., Tohoku Univ.)
71
P-67
A. nidulans
AmyR
AmyR
GMM
A. nidulans
AmyR
ST
DamyR
AflR
ST
ΔamyR
ST
DamyR
GMM
AflR
ST
AflR
ST
ST
CreA
CreA
CreA
A. nidulans
AflR
ST
CreA
ST
CreA
AflR
DamyR
GMM
AmyR
AflR
DamyR
AmyR
ST
ST
GMM
DNA
AmyR
Transcription factor AmyR of A.nidulans represses sterigmatocystin biosynthesis on glucose minimal medium.
Yosuke Kamimura, Toshiaki Narukami, Motoyuki Shimizu, Shunsuke Masuo, Naoki Takaya
(Graduate School of Life and Environmental Sciences, Univ. of Tsukuba)
P-68
,
,
(Aspergillus oryzae)
,
,
1)
,
,DNA
DNA
oryzae
,
ligD
A.
pyrG
,
,
Terabayashi et al.: Fungal. Genet. Biol. (2010) 47, p953-61.
Analysis of kojic acid synthesis genes from Aspergillus oryzae
Motoaki Sano, Mistuko Dohmoto, Hideaki Koike1, Eiji Ohsima2, Kuniharu Tachibana2, Yoshitaka Higa2,
Masayuki Machida1, Shinichi Ohashi
(KIT, 1AIST, 2Sansho Seiyaku Co., Ltd)
72
P-69
Aspergillus niger NRRL 328
III
III
III
328
PKS
CsyA
csyA
CsyA
36
An-csyA
III
PKS
An-cysA
A. niger NRRL 328
An-CsyA
RT-PCR
An-CsyA
2 20
An-CsyA
CoA
LC-MS/MS
CoA
2
An-CsyA
CoA
2 12
5
A. oryzae
3
2
An-CsyA
mRNA
His-tag
cDNA
CsyA
(PKS)
Aspergillus oryzae
A. niger NRRL
CsyA
CsyA
CsyA
III
2
14
CoA
An-
PKS
Production of pyrone compounds by a type III polyketide synthase from Aspergillus niger NRRL 328
Tatsuya Hamachi, Nozomi Miyai, Keiichi Kobayashi, Yuki Honda, Kohtaro Kirimura
(Dept. Appl. Chem., Fac. Sci. Eng., Waseda Univ.)
P-70
A. oryzae
III
1
1
1
csyB
1
2
1
,2
III
PKS
A. oryzae
csyB
4
amyB
III
csypyrone B1
α-pyrone
2
csypyrone B2, B3
ODS
HPLC
1.5 L
B2
PKS
A. oryzae M-2-3
ODS-
csypyrone B2
csypyrone B1
1
4.3 mg
B3
B3
III
PKS
13
C
2.8 mg
2
germicidin
csypyrone B
Functional analysis of type III polyketide synthase gene csyB from A. oryzae
Satomi Ishida, Makoto Hashimoto, Yasuyo Seshime, Katsuhiko Kitamoto, Isao Fujii
(1School of Pharmacy, Iwate Medical Univ., 2Dept. of Biotechnol., Univ. of Tokyo)
73
P-71
Pyripyropene
Pyr1
,
Pyripyropene A
Pyripyropene
Pyripyropene A
(Pyr1)
(Pyr2)
CoA
in vitro
Pyr1
Aspergillus fumigatus F37
CoA
AMP
Pyr1
cDNA
Aspergillus oryzae
CoA ligase
Pyripyropene A
Pyr1
GST
ATP
CoA
AMP
Pyr1
CoA
N-acetylcysteamine (NAC)
career protein (ACP)
acyl
Structure functional analysis of Pyr1 involved in the biosynthesis of Pyripyropene
Yoshihiko Shimokawa, Hiroyuki Morita, Ikuro Abe
(Graduate School of Pharmaceutical Science, The University of Tokyo)
P-72
terretonin
1
1
Aspergillus terreus
2
1
1
terretonin
1
pyripyropene
terretonin
PT
PKS
CYC
FMO
A.
terretonin
PKS
PKS
oryzae
PT
CYC
PKS
PT
FMO
CYC
FMO
PT
FMO
CYC
3
A.
CYC
MT
PT
2
farnesyl
pyripyropene
terreus
1
MT
PKS
FMO
A. oryzae
PKS
PT
CYC
Functional analysis of the biosynthetic genes involved in the biosynthesis of a fungal meroterpenoid terretonin
Yudai Matsuda1, Takayuki Itoh1, Kinya Tokunaga1, Isao Fujii2, Tetsuo Kushiro1, Yutaka Ebizuka1, Ikuro Abe1
(1Graduate School of Pharmaceutical Sciences, Univ. of Tokyo, 2School of Pharmacy, Iwate Medical Univ.)
74
P-73
2,
22Wagoner
Scorpinone
,
1
ACP
,
(R)
type I PKS
Fusarium solani
2-
F. solani
bostrycoidin
3-methylorcinaldehyde
,R
R
type I PKS (bos1)
Adenosine deaminase like protein (bos2)
Bos1
,
Bos2
bos1
bos2
Aspergius oryzae M-2-3
,
,
2-
in vitro
,
1: Van Wagoner, R.M. et al. J. Nat. Prod. (2008) 71, 426-430
Biosynthesis of 2-Azaanthraquinone
Takuya Kaji, Takayoshi Awakawa, Ikuro Abe
(Dept. of Pharmaceutical Sciences, Univ. of Tokyo)
P-74
peroxide
1
Verruculogen
2
1
verruculogen
1
FtmF
1
Aspergillus fumigatus
2
fumitremorgin
peroxide
fumitremorgin
α−
ftmF
FtmF
peroxide
FtmF
His
FtmF
fumitremorgin B
fumitremorgin B
Fe2+
α-
verruculogen
α−
PEG4000
X
1.82Å
FtmF
FtmF
FtmF
synthase-like
double-stranded β-helix
Functional characterization of verruculogen synthase FtmF of Aspergillus fumigatus
Naoki Kato1, Hideo Okumura2, Shunji Takahashi1, Hiroyuki Osada1
(1Chemical Biology Dept., RIKEN ASI, 2JASRI)
75
Clavaminate
P-75
1, 2
1
1
1
2
1
1
2
Fusarium sp. RK97-94
p53
p53
p53
p53
C
PKS-NRPS hybrid
Fusarium sp.
FUSS
C
PKS
AT
FUSS
AT
Genome Walking
methyltransferase
Analysis of the lucilactaene biosynthetic gene cluster
Akira Tanaka1, 2, Takayuki Motoyama1, Toshiaki Hayashi1, Hiroshi Hirota1, Naoko Takahashi-Ando2, Hiroyuki Osada1
(1Chemical Biology Dept., RIKEN
2
Graduate School of Engineering, Univ. of Toyo)
P-76
Coleophoma empetri F-11899
FR901379
Coleophoma empetri F-11899
T-DNA
FR901379
ATMT
FR901379
77-6D
DNA
TAIL-PCR
LB
1
T-DNA
DNA
RB
FR901379
2
T-DNA
2
T-DNA
4
FR901379
77-6D
T-DNA
DNA
77-6D
DNA
PCR
4
PCR
77-6D
DNA
Cla I
Eco RV
4
Investigation of inserted T-DNA regions in FR901379 non-producing mutant of fungus Coleophoma empetri F11899
Chikako Sakai, Masato Yamada, Yhosuke Orino, Yasuhiro Isogai, Seiji Hashimoto
(Dept. of Biotechnol., Toyama Pref. Univ.)
76
P-77
(
)
(Magnaporthe oryzae)
14
(RXM)
RXM
RXM
RXM
RXM
RXM
A Possible alternative Target of Roxithromycin in Fungi
Akira Ishii
Mayu Kumasaka
Yuuki Koizumi
Yuuya Kakumu
Takashi Kamakura
(Tokyo Univ. of science)
P-78
CBP1,CBL1
Magnaporthe oryzae
CBP1(chitin binding protein 1)
CBP1
CBP1
CBP1
CBL1
CBL1 (CBP1 like gene 1)
CBP1CBL1
CBP1
CBL1
CBL1
CBP1
CBP1CBL1
CBP1 CBL1
The role of Magnaporthe oryzae Chitin-Binding Protein Genes, CBP1 and CBL1, in Appressorium Differentiation
Sho Yoshida, Yuko Ohno, Yuichi Nakajima, Takashi Kamakura
(Dept. of Applied Biological Sci., Tokyo Univ. of Science)
77
P-79
B51
Magnaporthe oryzae
,
B51
B51
DNA
FHA
B51
,
B51
,
,
,
Functional analysis of germ tube expressing cDNA library of Magnaporthe oryze
Kengo Sasaki, Yuki Koizumi, Yuya Kakumu, Tsukasa Seida, Takashi Kamakura
(Dept. of Applied Biological Sci., Tokyo univ. of Sci.)
P-80
(
3
)
G
G
RGS
Regulators of G-protein Signaling
RGS
Rgs2
G
rgs2
rgs2
rgs2
G
RNA
Rgs2
Rgs2
25
rgs2
Screening of genes related to production of an elicitor-like agent in Magnaporthe oryzae.
Ayumu Sakaguchi, Toshiaki Harashima, Marie Nishimura.
(NIAS)
78
P-81
DNA
*
*
(Magnaprothe oryzae)
(HR)
HR
HR
(
22
HR
HR
DNA
DSBs
I-Sce I
YFP
)
(DSBs)
HR
DSBs
18
DNA
I-Sce I
DNA
DSBs
I-Sce I
I-Sce I
YFP
I-Sce I
DSBs
I-Sce I
HR
I-Sce I
YFP
DSBs
DSBs
HR
Induction of artificial DNA double strand breaks and homologous recombination repair in Rice blast fungus
Takayuki Arazoe, Shuichi Ohsato, * Tsutomu Arie, Katsuyoshi Yoneyama, Shigeru Kuwata
(Sch. Agric., Meiji Univ.; *Grad. Sch. Agric. Sci., Tokyo Univ. of Agric. & Tech.)
P-82
MoCV2
MoCV1
Magnaporthe oryzae
S-0412c
2
Magnaporthe oryzae chrysovirus 2(MoCV2) Magnaporthe oryzae endogenousvirus
1(MoEV1)
MoCV2
2.8-3.6kb
4-5
2
RNA
MoEV1
1.2-2.4kb
4
2
RNA
MoCV2
S-0412a
Magnaporthe oryzae chrysovirus 1(MoCV1)
:99%
:99%
MoCV2
S-0412c
MoCV1
S-0412a
MoCV2
MoCV1
MoCV2
MoCV1
35nm
130kDa 70kDa 65kDa
MoCV2
MoCV1
58kDa
MoCV2
MoCV2
SDS-PAGE
4
MoCV2
MoCV1
1/10
2
Comparison between two related mycoviruses, MoCV2 and MoCV1, infecting Magnaporthe oryzae
Tomoya Higashiura, Syunichi Urayama, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka,
Hiromitsu Moriyama
(Faculty of Agriculture, Tokyo Univ. of Agri.
79
Tech.)
P-83 (O-13)
MoCV1
Magnaporthe oryzae chrysovirus 1(MoCV1)
4~5
RNA
MoCV1
MoCV1
MoCV1
20
MoCV1
2
SDS-PAGE
14
MoCV1
Functional analysis of viral proteins of MoCV1 (Magnaporthe oryzae chrisovirus 1) which confers hypovirlence
traits to rice blast fungus.
Tokomo Ohta, Syunichi Urayama, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu Moriyama.
(Dept. Agric., Tokyo Univ. of Agric. & Tech.)
P-84
MoCV3 (Magnaporthe oryzae chrysovirus
3)
S-0412-
2a
MoCV1
RNA
dsRNA
Magnaporthe oryzae chrysovirus 1
MoCV1
MoCV3
Magnaporthe oryzae chrysovirus 3
MoCV3
MoCV1
1.36-1.39g/ml
35nm
SDS-PAGE
4
P130 P70 P65 P58
S-0412-
2a
Characterization of MoCV3 (Magnaporthe oryzae chrysovirus 3) that confers hypovirulence to rice blast fungus.
Hirofumi Sakoda, Syunichi Urayama, Ryoko Takai, Toshiyuki Fukuhara, Tsutomu Arie, Tohru Teraoka, Hiromitsu
Moriyama
(Faculty of Agriculture, Tokyo Univ. of Agri. & Tech)
80
P-85 (O-12)
Magnaporthe grisea
1
BJ-AFP1
(ROS)
BJ-AFP1
Pichia pastoris
ROS
BJ-AFP1
Magnaporthe grisea
M. grisea
BJ-AFP1
M. grisea
15
ROS
15
BJ-AFP1
BJ-AFP1
BJ-AFP1
BJ-AFP1
BJ-AFP1
BJ-AFP1
The mechanism of action of the plant defensin BJ-AFP1 in Magnaporthe gisea
Yoshifumi Oguro, Harutake Yamazaki, Masayuki Nashimoto, Masamichi Takagi, Hiroaki Takaku
(Applied Life Sci, Niigata Univ. of Pharmacy and Applied Life Science)
P-86
Phomopsis sp. (Diaporthe sp.)
Bipolaris oryzae
300-400 nm
rDNA
sp. (Diaporthe sp.)
ITS
Phomopsis
D1
D1
D1
D1
PDA
D1
D1
D1
Conidiation-promoting fungus Phomopsis sp. (Diaporthe sp.) against rice brown spot fungus Bipolaris oryzae
Yusuke Ohnishi, Makoto Ueno, Sakae Arase, Junichi Kihara
(Fac. Life Env. Sci, Shimane Univ.)
81
P-87
(Cochliobolus heterostrophus)
*
NADPH Oxidase
*
NADPH Oxidase
(O2-)
NADPH Oxidase
NADPH Oxidase
(Cochliobolus heterostrophus)
NADPH Oxidase
3
NADPH Oxidase
(Nox1, Nox2, Nox3)
Nox1
Nox2
Nox3
Nox2
Nox1
Nox3
Nox2
Nox1
Nox2
Characterization of NADPH oxidase genes in Cochliobolus heterostrophus.
*Kosuke Izumitsu, Yoshimoto Saitoh, Takuya Sumita , Atsushi Morita, Chihiro Tanaka
(Grad. School of Agriculture, Kyoto Univ., * Research Fellow of the Japan Society for the Promotion of Science)
P-88
Cdc42
,
,
Cochliobolus heterostrophus
CHK1-MAPK
Nox2
CHK1-MAPK
CHK1-MAPK
Ste20
Saccharomyces cerevisiae
MAPKK
Ste20
Rho
CHK1-MAPK
ChSte11
Ste11
Ste11
MAPKKK
small GTPase Cdc42
MAPKK
Cdc42
ChCdc42
Characterization of Cdc42 gene of Cochliobolus heterostrophus
Takuya Sumita, Kosuke Izumitsu, Atsushi Morita, Chihiro Tanaka
82
(Grad. School of Agriculture, Kyoto Univ.)
P-89
NIS1
Studies on an effector protein NIS1 secreted by Colletotrichum orbiculare.
Hiroki Irieda, Kae Yoshino, Kei Hiruma, Tetsuro Okuno, and Yoshitaka Takano
(Grad. School of Agriculture, Kyoto Univ.)
P-90 (O-14)
LAC2
Colletotrichum orbiculare
LAC2
LAC2
GFP
LAC2
LAC2
lac2
lac2
Magnaporthe oryzae
LAC2
lac2
LAC2
LAC2
LAC2
Isolation and Functional Analysis of a Secreted Protein Gene LAC2 Required for Fungal Pathogenicity of
Colletotrichum orbiculare
ShaoYu Lin, Shiho Okuda, Tetsuro Okuno, Yoshitaka Takano
83
(Grad. School of Agriculture, Kyoto Univ.)
P-91 (O-15)
Cryphonectria parasitica RAS3
chestnut blight
American chestnut
Cryphonectria parasitica
1900
C. parasitica
C. parasitica
RAS3
C
Rin
Rit
RAS
RAS1
RAS2
RAS3
Ras
CAAX Box
RAS3
ras3
GFP
RAS3
C
32
RAS3
C
32
RAS3
GTP
GTPase
RAS3
Biochemical and physiological functions of Cryphonectria parasitica RAS3
Yuki Yamauchi, Takuya Takahashi and Shin Kasahara
(Dept. of Env. Sci., Miyagi Univ.)
P-92
,
,
,
(Reactive Oxygen Species: ROS)
ROS
2
NADPH oxidase (Nox)
noxA
noxB
nox
noxB
gfp
noxB
GFP
GFP
NoxB
NoxB
The functional analysis of Nox complex in infection structures of Alternaria alternata Japanese pear pathotype
Yuichi Morita, Gang-Su Hyon, Kyoko Morikawa, Ken-ichi Ikeda, and Pyoyun Park
(Dept. of Agriculture, Univ. of Kobe)
84
P-93
A. oryzae
AoSO
Stress Granule
mRNA
stress granule
1)
A. oryzae AoSO
A. oryzae
stress granule
Saccharomyces cerevisiae Pab1p
EGFP
AoPab1-EGFP
AoPub1-EGFP
AoSO
Pub1p
A. oryzae
AoPab1-mDsRed
granule
AoSO
AoPab1, AoPub1
AoSO-EGFP
AoSO
stress
stress granule
1) J. Maruyama et al. (2010) Biochem. Biophys. Res. Commun. Vol. 391, 868-873.
Localization Analysis of AoSO and Stress Granule in Aspergillus oryzae
Hsiang Ting HUANG, Jun-ichi MARUYAMA, Katsuhiko KITAMOTO
85
(Dept. of Biotechnol., Univ. of Tokyo)
·························································· 81
·························································· 43
·························································· 68
·························································· 71
·························································· 79
····················································· 31, 69
······················································· 62
·························································· 67
·························································· 70
·························································· 18
·························································· 65
···················································· 41
·························································· 77
····················································· 30, 68
·························································· 45
·························································· 73
·························································· 82
·························································· 55
······················································· 65
····················································· 37, 46
·························································· 36
····················································· 27, 54
······················································· 56
·························································· 83
·························································· 42
·························································· 41
·························································· 52
·························································· 44
·························································· 52
·························································· 48
······················································· 76
·························································· 78
·························································· 28
·························································· 63
·························································· 80
·························································· 49
······················································· 78
·························································· 38
·························································· 62
·························································· 72
·························································· 39
·························································· 12
····················································· 32, 80
·························································· 71
·························································· 17
····················································· 31, 81
·························································· 36
·························································· 26
·························································· 74
·························································· 75
····················································· 27, 51
·························································· 75
·························································· 24
·························································· 72
·························································· 50
····················································· 28, 58
·························································· 34
·························································· 51
·························································· 45
·························································· 82
·························································· 47
·························································· 39
·························································· 56
·························································· 66
·························································· 43
·························································· 34
86
·························································· 76
·························································· 30
····················································· 35, 60
·························································· 53
·························································· 74
······················································· 59
·························································· 15
·························································· 54
·························································· 48
·························································· 21
·························································· 46
·························································· 58
······················································· 61
·························································· 84
·························································· 66
·························································· 33
·························································· 40
············································· 59
····················································· 26, 49
····················································· 33, 84
····················································· 35, 60
·························································· 40
·························································· 67
·························································· 29
······················································· 70
·························································· 50
·························································· 57
·························································· 47
·························································· 73
·························································· 38
·························································· 37
85
·························································· 69
·························································· 63
·························································· 77
·························································· 61
·························································· 79
·························································· 44
·························································· 53
····················································· 32, 83
...................................................................... 85
·························································· 42
·························································· 64
·························································· 57
····················································· 29, 64
·························································· 55
87
Fungal Molecular Biology Society of Japan
Conference on Fungal
Genetics and Molecular Biology
88
2010
89