Comments
Description
Transcript
ymc application data - MZ-AT
1 ビタミン Vitamins 2 糖類 Carbohydrates 3 YMC APPLICATION DATA 核酸 Nucleic acids 4 アミノ酸、ペプチド、 タンパク質 Amino acids, Peptides, Proteins 5 目 次 有機酸、脂肪酸誘導体 Organic acids, Fatty acid derivatives Contents 6 食品添加物 Food additives 分野別インデックス List of Applications 2 7 天然物 Natural products APPLICATION DATA 1 .ビタミン Vitamins 8 2 .糖類 Carbohydrates 20 3 .核酸 Nucleic acids 34 4 .アミノ酸、ペプチド、タンパク質 Amino acids, Peptides, Proteins 39 9 5 .有機酸、脂肪酸誘導体 Organic acids, Fatty acid derivatives 52 医薬品、生体関連物質 Drugs,Metabolites 6 .食品添加物 Food additives 54 7 .天然物 Natural products 60 8 .ステロイド Steroids 71 9 .医薬品、生体関連物質 Drugs, Metabolites 81 8 ステロイド Steroids 10 抗菌剤、抗生物質 Antibiotics 11 10.抗菌剤、抗生物質 Antibiotics 100 11.農薬、環境関連物質 Pesticides, Environmental 111 12.その他 The others 118 12 13.光学異性体 Optical isomers 126 その他 The others Index of Analytes 131 化合物名インデックス 農薬、環境関連物質 Pesticides, Environmental 13 光学異性体 Optical isomers 1 分野別インデックス 1 ビタミン List of Applications Vitamins 脂溶性ビタミン Oil-soluble vitamins ビタミンＡ脂肪酸エステル Vitamin A fatty acid ester 8 レチノール Retinol 8 カロテン Carotene 8 β-カロテン β-Carotene 9 ビタミン D Vitamin D ビタミン D2 とビタミンD3 Vitamin D2 and Vitamin D3 トコフェロール Tocopherols トコフェロールおよび酢酸トコフェロール Tocopherol and tocopherol acetate 8 9 10 10,11,12 12 ビタミン K Vitamin K 水溶性ビタミン Water-soluble vitamins 12 市販ドリンク剤中の水溶性ビタミン A drink including vitamins アラントインおよび水溶性ビタミン Allantoin and water-soluble vitamins 尿素、アラントインおよび水溶性ビタミン Urea, allantoin and water-soluble vitamins 17 ビタミンB1 誘導体 Thiamine and its modifide compounds 17 13,14,15,16,17 13,14 17 リボフラビンおよび関連化合物 Riboflavin and related compounds 18 ニコチン酸誘導体 Nicotinic acid and derivatives 18 パントテニルアルコール Pantothenyl alcohol 18 補酵素A誘導体 Coenzyme A and derivatives 18 L-アスコルビン酸およびエリソルビン酸 L-Ascorbic acid and erythorbic acid 19 L-アスコルビン酸の2,4-DNPH誘導体 2,4-Dinitrophenylhydrazone of L-ascorbic acid 19 2 糖類 Carbohydrates 単糖及び二糖類 Monosaccharides and disaccharides 20 単糖類 Monosaccharides 20 不飽和コンドロニ糖 Unsaturated chondro-disaccharides 21 糖アルコール Sugar alcohols 21 パラチノースとパラチニット Palatinose and palatinit 21 糖類および甘味料の一斉分析 Sugars and sweeteners 22 市販キャンディー中の糖類 Sugars in hard candy 22 オリゴ糖 Oligosaccharides フルクトオリゴ糖 Fructooligosaccharides 市販清涼飲料水のオリゴ糖 Oligosaccharides of soft drink 23 マルトオリゴ糖(G1 ∼G7) Maltooligosaccharides(G1 ∼G7) 24 マルトオリゴ糖(G11 ∼G15) Maltooligosaccharides(G11 ∼G15) マルトオリゴ糖(G1 ∼G15) Maltooligosaccharides(G1 ∼G15) サイクロデキストリン Cyclodextrins キチンオリゴ糖 N-Acetyl-chitooligosaccharides 28 ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains 28,29 ポリデキストロース Polydextrose 29 プルラン Pullulans 30 コンドロイチン Chondroitin 31 コンドロイチン硫酸 Ａ Chondroitin sulfate A 31 コンドロイチン硫酸 Ｂ Chondroitin sulfate B 31 コンドロイチン硫酸 Ｃ Chondroitin sulfate C 31 ヒアルロン酸 Hyaluronic acid 32 ヘパリン Heparin 32 ヘパラン硫酸 Heparan sulfate 32 ケラタン硫酸 Keratan sulfate 32 ケラタンポリ硫酸 Keratan polysulfate 33 2 22,23,24 23 25,26 26 26,27,28 INDEX 3 核酸 Nucleic acids 核酸塩基・ヌクレオシド Nucleosides and bases 34 核酸塩基 Nucleic acid bases 34 オロチン酸 Orotic acid 35 ヌクレオシド Nucleosides 35 ヌクレオチド Nucleotides 35,36 オリゴヌクレオチド Oligonucleotides オリゴヌクレオチド，poly(A) Poly(A) 37 糖ヌクレオチド Sugar nucleotides 37 プラスミドpBR322の制限酵素Msp I分解物 Plasmid pBR322 restriction fragments 38 4 アミノ酸、ペプチド、タンパク質 36 Amino acids, Peptides, Proteins アミノ酸 Amino acids 39 市販ドリンク剤中のタウリン Taurine in nutritious drink 39 PTC-アミノ酸 PTC-amino acids 39 PTH-アミノ酸 PTH-amino acids 39 NBD-アミノ酸 NBD-amino acids 40 酸化型および還元型グルタチオン Glutathione, oxidized and reduced form 40 ペプチドマップ Peptide mapping 40 アンジオテンシン Angiotensins インスリン Insulins ペプチド Peptides ペプチド -LC/MS Peptides -LC/MS ペプチドおよびタンパク質 Peptides and proteins タンパク質 Proteins ヒト免疫グロブリン Human immunoglobulins 41 41,42 42,43,44,45,49 43 45 45,46 46,47,48 チトクローム c Cytochrome c 48 ミオグロビン分解物 Peptide fragments from myoglobin 48 グリシンオリゴマー Glycine oligomers 49 カゼイン Casein 50 タンパク分子量マーカー Proteins for molecular weight marker 5 有機酸、脂肪酸誘導体 50,51 Organic acids, Fatty acid derivatives 有機酸 Organic acids 52 ピルビン酸 Pyruvic acids 52 不飽和脂肪酸メチルエステル Unsaturated fatty acid methyl esters 53 リノレン酸メチル異性体 Methyl linolenate isomers 53 トリアシルグリセロール Triacylglycerols 53 3 分野別インデックス 6 食品添加物 List of Applications Food additives 食品保存料 Food preservatives 54 パラオキシ安息香酸エステル類 p-Hydroxybenzoic acid esters 55 EDTAキレート EDTA-chelates 55 酸化防止剤 Antioxidants タール色素 Color additives 55,56,57 57 水溶性アナトー Annatto extract 市販炭酸飲料中のアスパルテーム Aspartame in a soft drink ステビオサイドとレバウディオサイドA Stevioside and rebaudioside A 59 グリチルリチン酸 Glycyrrhizic acid 59 7 天然物 58 58,59 Natural products アルカロイド Alkaloids アルカロイド-酢酸緩衝液 Alkaloids -acetate buffer 60 60 プリンアルカロイド Purine alkaloids 61 生薬成分（配糖体） Crude drugs(Glycosides) 61 ペオニフロリン Paeoniflorin 62 当帰芍薬散中のペオニフロリン Paeoniflorin in Toki-shakuyaku-san 62 トリテルペン系サポニン Saponins from roots of rubus suavissimus S.Lee 62 サイコサポニン Saikosaponins ジンセノサイド Ginsenosides 63 63,64 フラボノイド Flavonoids 64 クエルセチンとその配糖体 Quercetin and its glycosides 65 バイカリン Baicalin 65 フラバノン配糖体 Flavanone glycosides 65 市販みそ中のイソフラボン Isoflavones from miso, fermented soybean paste 66 カテキン類 Catechins 66 2-フェニルエチルグリコシド 2-Phenylethyl glycosides 67 センノシド Sennosides 67 アルブチンおよび関連化合物 Arbutin and related compounds ペオノール Paeonol 68 68,69 アフラトキシン Aflatoxins 69 クロロフィル Chlorophyll a and b 69 トウガラシ中のカプサイシン類 Capsaicinoids from red papper 70 トウガラシ中のカプサイシン類 -LC/MS Capsaicinoids from red papper -LC/MS 70 8 ステロイド Steroids ステロイド Steroids 副腎皮質ステロイド Adrenocorticosteroids ヒドロコルチゾンとプレドニゾン Hydrocortisone and prednisone 男性ホルモン Androgens テストステロン誘導体 Synthetic androgens 女性ホルモン Estrogens and progestins 71,74 71,72,73,74 72 75 75,76 76 エストラジオール誘導体 Estrogens ノルゲストレルとエチニルエストラジオール Norgestrel and ethinylestradiol 76,77 77 豚胆中の胆汁酸のフェナシルエステル誘導体 Phenacyl esters of bile acids from pig gall 77 エクダイソン Ecdysones 78 ステロール Sterols 78 強心配糖体 Cardiac glycosides 79 センソ成分 Steroids from toad venom 80 4 INDEX 9 医薬品、生体関連物質 Drugs, Metabolites 風邪薬成分 Ingredients in a cough/cold medication 81,82 市販風邪薬 A commercial cough/cold medication 81,82 市販鎮痛解熱剤 Pain-killing drugs 83 インドメタシン Indometacin 83 解熱鎮痛薬成分 Analgesics 83 解熱鎮痛薬 Pain-killing drugs 84 コデイン Codeine 84 市販点鼻薬 A commercial nasal spray 84 鼻炎用点鼻剤 Collunarium 85 塩化ベンゼトニウム Benzethonium chloride 85 塩化ベンザルコニウム Benzalkonium chlorides 85 殺菌剤 Disinfectans 86 クロルヘキシジン Chlorhexidine 86 鼻炎薬成分 Ingredients in a drug for rhinitis 86 点眼剤成分 Ingredients in an eye drop 86 抗ヒスタミン薬 Histamine antagonists 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 酸性非ステロイド性解熱鎮痛抗炎症薬 -LC/MS Acidic non-steroidal anti-inflammatory drugs -LC/MS 三環系抗うつ薬 Tricyclic antidepressants 抗精神病薬 Antipsychotic drugs 89 抗てんかん薬 Antiepileptics 89 抗高脂血症剤 Hypolipidemic drugs 90 ロバスタチン Lovastatin 90 酸性および塩基性医薬品(pH6.8) Acidic and basic drugs(pH6.8) 91 酸性および塩基性医薬品(pH8.8) Acidic and basic drugs(pH8.8) 91 カルシウム拮抗薬 Calcium antagonists 91,92 不整脈治療薬 Antiarrhythmic drugs 92 局所麻酔薬 Local anesthetics 93 利尿薬 Diuretics 93 87 87,88 88 88,89 制がん剤および関連化合物 Antineoplastic and related compounds 94 葉酸および葉酸拮抗物質 Folic acid and its antagonists 94 抗HIVヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents ニコチン酸構造類縁体 Nicotinic acid analogues 94,95 塩基性化合物 Basic compounds 96 殺菌薬 Disinfectants 96 95 アシクロビル Acyclovir 96 塩化カルニチン Carnitine chlorides 96 キサンチンオキシターゼ阻害剤および関連化合物 Xanthine oxidase inhibitor and related compounds 97 カフェイン代謝物 Caffeine metabolites 97 尿中のカフェイン代謝物 Caffeine metabolites in human urine 97 プリン代謝物 Purine catabolites 98 カテコールアミン Catecholamines 馬尿酸類 Hippuric and methylhippuric acids 98 98,99 5 分野別インデックス 10 抗菌剤、抗生物質 List of Applications Antibiotics 合成抗菌剤 Antibacterial agents サルファ剤 Sulfonamides 抗生物質 Antibiotics ナリジクス酸系抗生物質 Nalidixic acid antibiotics セフェム系抗生物質 Cephalosporin antibiotics セファロスポリンC Cephalosporin C セフトリアキソン Ceftriaxone セフォキシチンナトリウム Cefoxitin sodium ペニシリン系抗生物質 Penicillin antibiotics 100 100,101,102 102 102,103 103,104,105,106,107 108 108 108,109 109 アモキシシリン Amoxicillin 109 抗生物質の高速分離 -LC/MS Rapid separation of antibiotics -LC/MS 110 エリスロマイシン Erythromycin antibiotic drugs 110 テトラサイクリン系抗生物質 Tetracycline antibiotics 110 バンコマイシン Vancomycin 110 11 農薬、環境関連物質 Pesticides, Environmental ベンスリド Bensulide 111 チウラム Thiram 111 アシュラム Asulam 111 オキシン銅 Copper 8-quinolinolate 111 水質規制農薬9成分 Analysis of 9 pesticides 112 除草剤 Herbicides 112 アトラジン誘導体 Atrazine derivatives トリアジン系除草剤 Triazine herbicides 112 112,113 尿素系除草剤 Urea herbicides 113 酸アミド系除草剤 Acid amide herbicides 113 カルバメート系除草剤 Carbamate herbicides 113 カルバメート系殺虫剤 Carbamate insecticides 114 防かび剤 Fungicides 114 イマザリル Imazalil 114 陰イオン界面活性剤 Anionic surfactants 115 市販洗濯洗剤中の陰イオン界面活性剤 Anionic surfactants in a commercial laundry detergent 115 市販クレンザー中の陰イオン界面活性剤 Anionic surfactants in a commercial cleanser 非イオン性界面活性剤 Nonionic surfactants ポリエチレングリコール Polyethyleneglycols アルデヒドおよびケトン類の2,4-DNPH誘導体 2,4-Dinitrophenylhydrazones of aldehydes and ketones 116 多環芳香族炭化水素 Polyaromatic hydrocarbons 117 6 115 115,116 116 INDEX 12 その他 The others ヒドロキシ安息香酸 Hydroxybenzoic acids 118 レゾルシン酸異性体 Resorcylic acid isomers 118 安息香酸誘導体 Benzoic acid derivatives 118,119 メトキシ安息香酸異性体 Methoxybenzoic acid isomers クロロ安息香酸異性体 Chlorobenzoic acid isomers フタル酸異性体 Phthalic acid isomers ポリフェノール Polyphenols 120 ジメチルフェノール異性体 Dimethylphenol isomers 121 フェノール誘導体 Phenol derivatives 119 119,120 120 121,122 クレゾール異性体 Cresol isomers 122 クロロフェノール異性体 Chlorophenol isomers 122 クロロアセトフェノン異性体 Chloroacetophenone isomers 123 ターフェニル異性体 Terphenyl isomers 123 フラーレン同族体 Fullerenes 123 1- (2-ピリジルアゾ) -2-ナフトール(PAN)金属キレート Metal-PAN chelates 124 配位性化合物 Coordination compounds 124 ヒノキチオール Hinokitiol 124 アミノアルコール Aminoalcohols 124 尿素及び関連化合物 Urea and related compounds 125 芳香族アミン Aromatic amines 125 13 光学異性体 Optical isomers ビナフトール 1,1'-Bi-2-naphthol トリフルオロアンスリルエタノール 2,2,2-Trifluoro-1-(9-anthryl)-ethanol ヘキソバルビタール Hexobarbital 126 126 126,128 CBZ-フェニルアラニン CBZ-Phenylalanine(Z-Phe-OH) 塩酸プロプラノロール Propranolol hydrochloride 126,129,130 塩酸ケタミン Ketamine hydrochloride 127 クロルタリドン Chlorthalidone 127 フェノプロフェンカルシウム Fenoprofen calcium salt; hydrate フルルビプロフェン Flurbiprofen イブプロフェン Ibuprofen 128 インダパミド Indapamide 128 127,130 127 128,129 ケトプロフェン Ketoprofen 129 ナフチルエチルアルコール 1-(1-Naphthyl)-ethylalcohol 129 フェニルエチルアルコール Phenylethylalcohol 130 トレーガー塩基 Troger's base 130 7 ビタミン 1 1 Vitamins ビ タ ミ ン 脂溶性ビタミン Oil-soluble vitamins ビタミンＡ脂肪酸エステル Vitamin A fatty acid ester 1. Menadione (Vitamin K3) 2. Retinol (Vitamin A) 3. Retinol acetate 4. Menaquinone (Vitamin K2) 5. δ-Tocopherol 6. Ergocalciferol (Vitamin D2) 7. Cholecalciferol (Vitamin D3) 8. α-Tocopherol (Vitamin E) 9. α-Tocopherol acetate 10. Phylloquinone (Vitamin K1) 1. Retinol palmitate (Vitamin A palmitate) (G910415B) (G910523T) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.08 AUFS Injection : 15 μL (0.01∼0.21 mg/mL) : YMC-Pack SIL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : cyclohexane/dichloromethane/acetic acid (100/0.5/0.5) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 350 nm, 0.32 AUFS Injection : 5 μL (1.0 mg/mL) レチノール Retinol カロテン Carotene Column 1. α-Carotene 2. β-Carotene 1 Retinol (A970708A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (92/8) Flow rate : 1.0 mL/min Temperature : 35 ℃ Detection : UV at 325 nm, 0.02 AUFS Injection : 20 μL (0.5 μg/mL) 8 (G910523Q) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/THF (50/45/5) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : VIS at 450 nm, 0.16 AUFS Injection : 5 μL (0.05 mg/mL) ビタミン 1 Vitamins 1 Vitamins β-カロテン β-Carotene β-カロテン β-Carotene 1. β-Carotene 1. β-Carotene (M910529A) (A970716K) Column : YMC-Pack CN (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water/THF (35/35/30) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : VIS at 450 nm, 0.32 AUFS Injection : 7 μL (0.125 mg/mL) Column : YMC-Pack ODS-AL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/chloroform (96/4) Flow rate : 1.5 mL/min Temperature : 35 ℃ Detection : VIS at 455 nm, 0.04 AUFS Injection : 20 μL (4.0 μg/mL) ビタミン D Vitamin D ビタミン D Vitamin D 1 1 Ergocalciferol (Vitamin D2) Cholecalciferol (Vitamin D3) (C970808E) Column : YMC-Pack SIL (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : hexane/2-propanol (99.6/0.4) Flow rate : 1.6 mL/min Temperature : ambient (28 ℃) Detection : UV at 265 nm, 0.01 AUFS Injection : 100 μL (1.0 μg/mL) (C970808H) Column : YMC-Pack SIL (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : hexane/2-propanol (99.6/0.4) Flow rate : 1.6 mL/min Temperature : ambient (28 ℃) Detection : UV at 265 nm, 0.01 AUFS Injection : 100 μL (1.0 μg/mL) 9 ビタミン 1 1 Vitamins ビ タ ミ ン ビタミンD2 とビタミンD3 Vitamin D2 and vitamin D3 ビタミンD2 とビタミンD3 Vitamin D2 and vitamin D3 (P040818A) (P040819C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (95/5) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 265 nm Injection : 10 μL (0.01 mg/mL) Column : YMC-Pack ODS-AL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (95/5) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 265 nm Injection : 10 μL (0.01 mg/mL) トコフェロール Tocopherols トコフェロール Tocopherols 1. α-Tocopherol 2. β-Tocopherol 3. γ-Tocopherol 4. δ-Tocopherol 1 α-Tocopherol 2 β-Tocopherol 3 γ-Tocopherol 4 δ-Tocopherol (G910523S) Column : YMC-Pack SIL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : hexane/THF/acetic acid (97/3/0.25) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 295 nm, 0.08 AUFS Injection : 10 μL (0.20∼0.31 mg/mL) 10 (S970702A) Column : YMC-Pack SIL (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : hexane/2-propanol/acetic acid (1000/6/5) Flow rate : 1.4 mL/min Temperature : 35 ℃ Detection : FLS at Ex 298 nm, Em 325 nm Injection : 20 μL (5∼20 μg/mL) ビタミン 1 Vitamins 1 Vitamins トコフェロール Tocopherols トコフェロール Tocopherols 1. α-Tocopherol 2. β-Tocopherol 3. γ-Tocopherol 4. δ-Tocopherol 1 α-Tocopherol 2 β-Tocopherol 3 γ-Tocopherol 4 δ-Tocopherol (T910501A) (T920728E) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : n-hexane/tetrahydrofuran/acetic acid/water (93/7/0.4/0.01) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 295 nm, 0.08 AUFS Injection : 7 μL (0.25 mg/mL) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : hexane/ethyl acetate (70/30) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 295 nm, 0.08 AUFS Injection : 7 μL (0.25 mg/mL) トコフェロール Tocopherols トコフェロール Tocopherols 1 1 δ-Tocopherol δ-Tocopherol 2 2 γ-Tocopherol γ-Tocopherol 3 3 β-Tocopherol β-Tocopherol 4 4 α-Tocopherol α-Tocopherol (S921217A) (S930726A) Column Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : 2-propanol/water (68/32) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 295 nm, 0.032 AUFS Injection : 7 μL (0.25 mg/mL) : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 2-propanol/water (75/25) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 295 nm, 0.08 AUFS Injection : 7 μL (0.25 mg/mL) Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992) 11 ビタミン 1 1 Vitamins ビ タ ミ ン トコフェロール Tocopherols トコフェロールおよび酢酸トコフェロール Tocopherol and tocopherol acetate 1 1 δ-Tocopherol 2 α-Tocopherol γ-Tocopherol 2 3 β-Tocopherol α-Tocopherol acetate 4 α-Tocopherol (S921216C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 2-propanol/water (65/35) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 295 nm, 0.04 AUFS Injection : 7 μL (0.25 mg/mL) (S970426E) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (98/2) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 284 nm, 0.51 AUFS Injection : 20 μL (1 mg/mL) Ref.) Y.Satomura, M. Kimura and Y. Itokawa, J.Chromatogr., 625, 372-376 (1992) トコフェロールおよび酢酸トコフェロール Tocopherol and tocopherol acetate （日本薬局方第13改正記載条件） ビタミン K Vitamin K 1 1 α-Tocopherol Menaquinone (Vitamin K2) 2 2 α-Tocopherol acetate Phylloquinone (Vitamin K1) (S970426C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (98/2) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 284 nm, 0.51 AUFS Injection : 20 μL (1 mg/mL) （日本薬局方第13改正記載条件） 12 (A970728B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/ethanol (5/1) Flow rate : 0.8 mL/min Temperature : 40 ℃ Detection : FLS at Ex 320 nm, Em 430 nm reduction column (platinum black, 10 x 4.0 mmI.D.) Injection : 20 μL (100 ng/mL) ビタミン 1 Vitamins 1 水溶性ビタミン Water-soluble vitamins 1. L-Ascorbic acid (Vitamin C) 2. Nicotinic acid 3. Pyridoxine HCl (Vitamin B6) 4. Nicotinamide 5. Thiamine HCl (Vitamin B1) 6. Folic acid 7. Cyanocobalamin (Vitamin B12) 8. Riboflavin (Vitamin B2) 1. Nicotinic acid 2. Pantothenic acid (Na salt) 3. Pyridoxine HCl (Vitamin B6) 4. Nicotinamide 5. Thiamine HCl (Vitamin B1) (G910415A) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)20mM KH2PO4 - K2HPO4 (pH 6.6) B)20mM KH2PO4 - K2HPO4 (pH 6.6)/acetonitrile (80/20) 0%B (0-5min), 0-100%B (5-20min, linear), 100%B (20-25min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 10 μL (0.04∼0.31 mg/mL) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NaH2PO4 - Na2HPO4 (pH 6.5) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.08 AUFS Injection : 5 μL (0.02∼0.58 mg/mL) 水溶性ビタミン Water-soluble vitamins 市販ドリンク剤中の水溶性ビタミン A drink including vitamins (G910523O) Column 1. L-Ascorbic acid (Vitamin C) 2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide 4. Panthenol 5. Caffeine 6. Flavin mononucleotide sodium salt (Vitamin B2) 3 1 1. L-Ascorbic acid (Vitamin C) 2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide 4. Panthenol 5. Caffeine 6. Flavin mononucleotide sodium salt (Vitamin B2) 5 5 1 3 2 4 6 2 6 4 0 10 20 30 0 min (F001004B) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3PO4(pH3.5) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) 0-60%B(0-20min), 60%B(20-30min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.32AUFS Injection : 5 μL (0.05 ∼ 0.40 mg/mL) 10 20 30 min (F001004A) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3PO4(pH3.5) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) 0-60%B(0-20min), 60%B(20-30min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.32AUFS Injection : 5 μL Sample : 5 times dilution with 5% metaphosphoric acid 13 Vitamine 水溶性ビタミン Water-soluble vitamins ビタミン 1 1 Vitamins ビ タ ミ ン 水溶性ビタミン Water-soluble vitamins 1 市販ドリンク剤中の水溶性ビタミン A drink including vitamins 1. Thiamine HCl (Vitamin B1) 2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide 4. Caffeine 5. Flavin mononucleotide sodium salt (Vitamin B2) 3 1. Thiamine HCl (Vitamin B1) 2. Pyridoxine HCl (Vitamin B6) 3. Nicotinamide 4. Caffeine 5. Flavin mononucleotide sodium salt (Vitamin B2) 4 4 2 3 5 1 5 2 0 10 20 30 0 min 10 20 30 min (F001011B) (F001011A) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3PO4(pH3.5) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) 0-90%B(0-20min), 90%B(20-30min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32AUFS Injection : 15 μL (0.04∼0.60 mg/mL) 水溶性ビタミン Water-soluble vitamins Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3PO4(pH3.5) B)50mM KH2PO4-H3PO4(pH3.5)/acetonitrile (80/20) 0-90%B(0-20min), 90%B(20-30min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32AUFS Injection : 15 μL Sample : 5 times dilution with water 水溶性ビタミン Water-soluble vitamins 1.p-Aminobenzoic acid (PABA) 2.Biotin (Vitamin H) 3.Riboflavin (Vitamin B2) 4.Cyanocobalamin (Vitamin B12) 1.p-Aminobenzoic acid (PABA) 2.Biotin (Vitamin H) 3.Cyanocobalamin (Vitamin B12) 4.Riboflavin (Vitamin B2) (M920212B) (M920212A) : YMC-Pack Ph (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 10 μL (0.02∼0.30 mg/mL) 14 Column Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 10 μL (0.02∼0.30 mg/mL) ビタミン 1 Vitamins 1 水溶性ビタミン Water-soluble vitamins 1.p-Aminobenzoic acid (PABA) 2.Biotin (Vitamin H) 3.Riboflavin (Vitamin B2) 4.Cyanocobalamin (Vitamin B12) 1.p-Aminobenzoic acid (PABA) 2.Biotin (Vitamin H) 3.Riboflavin (Vitamin B2) 4.Cyanocobalamin (Vitamin B12) (M920212C) (M920212D) Column : YMC-Pack TMS (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 10 μL (0.02∼0.30 mg/mL) : YMC-Pack C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 10 μL (0.02∼0.30 mg/mL) 水溶性ビタミン Water-soluble vitamins 水溶性ビタミン Water-soluble vitamins Column Column 1.L-Ascorbic acid (Vitamin C) 2.Orotic acid (Vitamin B13) 3.Pyrroloquinoline quinone (PQQ) 4.Pyridoxine HCl (Vitamin B6) 5.Nicotinamide 1.L-Ascorbic acid (Vitamin C) 2.Orotic acid (Vitamin B13) 3.Pyrroloquinoline quinone (PQQ) 4.Pyridoxine HCl (Vitamin B6) 5.Nicotinamide (M920207B) (M920207A) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM CH3COOH/100mM CH3COONH4 (30/70, pH 5.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.04∼0.20 mg/mL) Column : YMC-Pack TMS (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM CH3COOH/100mM CH3COONH4 (30/70, pH 5.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.04∼0.20 mg/mL) 15 Vitamins 水溶性ビタミン Water-soluble vitamins ビタミン 1 1 Vitamins ビ タ ミ ン 水溶性ビタミン Water-soluble vitamins 水溶性ビタミン Water-soluble vitamins 1.Nicotinic acid 2.Erythorbic acid 3.L-Ascorbic acid 1.Nicotinic acid 2.Erythorbic acid 3.L-Ascorbic acid (T920525A) (T920525B) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 250 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.1 mg/mL) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 250 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.1 mg/mL) 水溶性ビタミン Water-soluble vitamins 水溶性ビタミン Water-soluble vitamins 1.Pyridoxine HCl 2.Nicotinamide 3.Benfotiamine 4.Cyanocobalamin 5.Riboflavin 1 Xanthine(I.S.) 2 Pyridoxal HCl 3 Pyridoxine HCl 4 Nicotinamide (S930712E) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM (NH4)2HPO4 (10/90) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.13 AUFS Injection : 5 μL (0.04∼0.48 mg/mL) 16 (S960912B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.13 AUFS Injection : 5 μL (0.05∼0.2 mg/mL) ビタミン 1 Vitamins 1 Vitamins 水溶性ビタミン Water-soluble vitamins アラントインおよび水溶性ビタミン Allantoin and water-soluble vitamins 1. COOH H2 N 1. 1 p -Aminobenzoic acid 2. COOH 2 Nicotinic acid H 3C 3 3. CHO HO 4 5 1 OH OH OH CH2CH CH CH CH2OH O N 2. N N 2 CONH2 N Nicotinamide 3 4 H 3C NH N O Riboflavin CH2OH CH3 N 3. H2NCONH ・HCl Pyridoxal HCl H N Allantoin CH2OH CH2OH 4. HO CH3 N 4. HO ・HCl CH2OH CH2OH CH3 N ・HCl Pyridoxine HCl Pyridoxine HCl 5. O NH O CONH2 N Nicotinamide 0 2 4 0 6 min 5 10 15 20 min (S010125A) (S971121D) Column : Hydrosphere C18 (3μm, 12nm) 50 × 2.0 mm I.D. Eluent : 10mM CH3COONH4 Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.128 AUFS Injection : 2 μL (0.01∼0.1 mg/mL) : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - H3PO4 (pH 2.5) / acetonitrile (20/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 8 μL (0.01∼0.04 mg/mL) 尿素、アラントインおよび水溶性ビタミン Urea, allantoin and water-soluble vitamins ビタミンB1 誘導体 Thiamine and its modified compounds Column 1 Thiamine HCl 1. 5. CH3 OH HOCH2C CH CONH(CH2)3OH CH3 CH2OH HCOH O HO Panthenol (Pantothenyl alcohol) O 2 OH L-Ascorbic acid (Vitamin C) Benfotiamine 2. 6. COOH Nicotinic acid 6 HO CH3 N CH2OH CH2OH 3 ・HCl N Pyridoxine HCl (Vitamin B6) 3. Dicethiamine HCl H2NCONH2 1 Urea 4 2 4. 5 H2NCONH 3 O H N 4 O NH Bisbentiamine Allantoin 0 5 10 15 20 min (S940202D) (J030624B) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4-H3PO4 (pH2.8)/acetonitrile (20/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 10 μL (0.01∼5.0 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : A)50mM (NH4)2HPO4 B)methanol/50mM (NH4)2HPO4 (60/40) 10-100%B (0-15min, linear), 100%B (15-30min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.26 AUFS Injection : 10 μL (0.1∼0.2 mg/mL) 17 ビタミン 1 1 Vitamins ビ タ ミ ン リボフラビンおよび関連化合物 Riboflavin and related compounds 1 ニコチン酸誘導体 Nicotinic acid and derivatives H 3C OH OH OH O CH2CH CH CH CH2O P O N O H 3C N N O P ONa NH O NH2 N N O CH2 N O 1 2 N OH OH H 3C OH OH OH O CH2CH CH CH CH2O P OH O N ONa H 3C N 2 N 5 NH O Flavin sodium phosphate (Flavin mononucleotide sodium salt) 3 3 OH OH OH CH2CH CH CH CH2OH O N 6 N H 3C H 3C NH N Riboflavin O 0 0 10 4 1 2 Flavin adenine dinucleotide disodium 3 1. Nicotinamide adenine dinucleotide phosphate oxidized form (NADP) 2. Nicotinic acid 3. Nicotinamide adenine dinucleotide phosphate reduced form (NADPH) 4. Nicotinamide 5. β-Nicotinamide adenine dinucleotide (NAD) 6. β-Nicotinamide adenine dinucleotide reduced form (NADH) ONa 5 10 15 20 25 min 20 min (S000803B) (F000707B) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM KH2PO4 - K2HPO4 (pH 5.9) / acetonitrile (90/10) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.1 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4-K2HPO4(pH6.9)/acetonitrile (99/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 6 μL (0.05∼0.10 mg/mL) パントテニルアルコール Pantothenyl alcohol 補酵素A誘導体 Coenzyme A and derivatives 1. Malonyl-CoA 2. 1 Coenzyme A glutathione disulfide 3. Coenzyme A Pantothenyl alcohol 4. 2 Methylmalonyl-CoA 5. Sccinyl-CoA Resorcinol(I.S.) 6. 3-Hydroxy-3-methylglutarylCoA (HMG-CoA) 7. Acetoacetyl-CoA 8. Acetyl-CoA 9. Coenzyme A oxidized 10. Propionyl-CoA Courtesy of Dr. Takamura, Ibaraki University (S961119B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (5/95) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.13 AUFS Injection : 5 μL (0.05∼0.4 mg/mL) 18 Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)methanol/50mM KH2PO4 (10/90) B)methanol/50mM KH2PO4 (30/70) 10%B (0-5min), 10-100%B (5-30min) Flow rate : 0.7 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.016 AUFS Injection : 10 μL (16.7 nmol/mL) (S940630E) ビタミン 1 Vitamins 1 L-アスコルビン酸及びエリソルビン酸 L-Ascorbic acid and erythorbic acid 1. Erythorbic acid 2. L-Ascorbic acid (Vitamin C) 1. L-Ascorbic acid (Vitamin C) 2. Erythorbic acid (T910425A) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.16 AUFS Injection : 10 μL (0.1 mg/mL) (G910523N) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM CH3COONH4 in 5mM tetrabutylammonium bromide Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.08 AUFS Injection : 5 μL (0.05 mg/mL) L-アスコルビン酸の2,4-DNPH誘導体 2,4-Dinitrophenylhydrazone of L-ascorbic acid 1. L-Ascorbic acid 2,4-DNPH (C970808K) Column : YMC-Pack SIL (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : hexane/ethyl acetate/2-propanol/acetic acid (40/30/2/1) Flow rate : 1.5 mL/min Temperature : 35 ℃ Detection : VIS at 540 nm, 0.01 AUFS Injection : 20 μL (10 μg/mL) 19 Vitamins L-アスコルビン酸及びエリソルビン酸 L-Ascorbic acid and erythorbic acid 糖類 1 2 Carbohydrates 糖 類 単糖及び二糖類 Monosaccharides and disaccharides 単糖及び二糖類 Monosaccharides and disaccharides 1.Fructose 2.Glucose 3.Sucrose 4.Maltose 5.Lactose 1.Fructose 2.Glucose 3.Sucrose 4.Maltose 5.Lactose (T910726A) (T910723A) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (75/25) Flow rate : 1.0 mL/min Temperature : 26 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 5 μL (14.3∼28.6 mg/mL) : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : ethanol/ethyl acetate/acetonitrile/water (30/40/5/25) Flow rate : 1.0 mL/min Temperature : 26 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 5 μL (14.3∼28.6 mg/mL) 単糖及び二糖類 Monosaccharides and disaccharides 単糖類 Monosaccharides Column 1.Fructose 2.Glucose 3.Sucrose 4.Maltose 5.Lactose 1.Rhamnose 2.Fucose 3.Xylose 4.Fructose 5.Mannose 6.Glucose 7.Galactose (T910718E) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : ethanol/ethyl acetate/acetonitrile/water (30/40/5/25) Flow rate : 1.0 mL/min Temperature : 35 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 5 μL (14.3∼28.6 mg/mL) 20 (T910718D) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. × 2 Eluent : ethanol/ethyl acetate/acetonitrile/water (30/30/23/17) Flow rate : 1.0 mL/min Temperature : 26 ℃ Detection : RI, 16 x 10-6 RIU/FS Injection : 25 μL (0.65∼115.6 mg/mL) 糖類 2 Carbohydrates 不飽和コンドロニ糖 Unsaturated chondro-disaccharides 糖アルコール Sugar alcohols 2 1.ΔDi-0S 2.ΔDi-6S 3.ΔDi-4S 4.ΔDi-diSD 5.ΔDi-diSE 6.ΔDi-triS Carbohydrates 1. Glycerol 2. Erythritol 3. Xylitol 4. Sorbitol 5. Maltitol 6. Lactitol 1 2 3 4 5 6 0 10 20 30 min (T910920A) (F030603A) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)0.01M NH4H2PO4 - (NH4)2HPO4(pH 6.7) B) 0.5M NH4H2PO4 - (NH4)2HPO4(pH 6.7) 0%B (0-5min), 0-100%B (5-30min,linear), 100%B (30-40min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 232 nm, 0.32 AUFS Injection : 9 μL (0.12∼0.24 mg/mL) : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (75/25) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : RI, 32 × 10-6 RIU/FS Injection : 5 μL (5.0 mg/mL) 糖アルコール Sugar alcohols パラチノースとパラチニット Palatinose and Palatinit Column 1. Glycerol 2. Erythritol 3. Xylitol 4. Ribitol 5. Arabitol 6. Sorbitol 7. Mannitol 8. Maltitol 9. Inositol 1. Palatinose 2. Palatinit (*) (Isomalt) 1 2 0 10 20 min (*)Mixture of 6-O-α-D-Glucopyranosyl-D-sorbitol and 1-O-α-D-Glucopyranosyl-D-mannitol (T910718B) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : ethanol/ethyl acetate/acetonitrile/water (30/30/25/15) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 15 μL (1.7∼3.3 mg/mL) (F030616B) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : RI, 64 x 10-6 RIU/FS Injection : 15 μL (5.0 mg/mL) 21 糖類 2 Carbohydrates 2 糖類および甘味料の一斉分析 Sugars and sweeteners 市販キャンディー中の糖類 Sugars in hard candy A) Standard 1. Sucralose 2. Erythritol 3. Xylitol 4. Fructose 5. Sorbitol 6. Glucose 7. Sucrose 8. Palatinose 9. Maltitol 10. Maltose 11. Lactitol 糖 類 1 2 1 2 3 4 5 7 6 0 F030617F 10 9 8 20 11 10 30 1. Sucralose 2. Erythritol 3. Xylitol 4. Fructose 5. Sorbitol 6. Glucose 7. Sucrose 8. Palatinose 9. Maltitol 10. Maltose 40 min 11. Lactitol B) Hard candy (Sample KM) 3 7 4 5 7 9 11 8 6 34 5 6 0 F030618A 0 10 20 9 2 10 30 10 10 20 30 40 min 40 min (F030618A) : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (75/25) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 5 μL (5.0 mg/mL) : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (75/25) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 5 μL Sample : A) 5 mg/mL B) 50 mg/mL (50% ethanol solution of candy) オリゴ糖 Oligosaccharides オリゴ糖 Oligosaccharides (F030613C) Column Column 1. 2. 1. 2. (A970625G) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : ambient (26 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (4.0 mg/mL) 22 (A970701G) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (4.0 mg/mL) 糖類 2 Carbohydrates フルクトオリゴ糖 Fructooligosaccharides オリゴ糖 Oligosaccharides 2 Carbohydrates 1. Frufβ2→1αGlc (Sucrose) 2. Frufβ2→1Frufβ2→1αGlc (1-Kestose) 3. Frufβ2→[1Frufβ2]2 →1αGlc (Nystose) 4. Frufβ2→[1Frufβ2]3 →1αGlc (1-Fructofuranosyl-D-nystose) 1 2 1. 2. 3 4 0 10 20 30 min (F000725B) (A970627F) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : RI, 64 x 10-6 RIU/FS Injection : 5μL (10 mg/mL) : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : ambient (26 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (4.0 mg/mL) 市販清涼飲料水のオリゴ糖 Oligosaccharides of soft drink オリゴ糖 Oligosaccharides Column 1. 2. 1. (Lactosyl fructoside) (A970630C) (A970628H) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 10 μL Sample : 10 times dilution with water Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : ambient (26 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (4.0 mg/mL) 23 糖類 2 Carbohydrates 2 オリゴ糖 Oligosaccharides マルトオリゴ糖(G1 ∼G7) Maltooligosaccharides(G1 ∼ G7) 糖 類 1. Glucose (G1) 2. Maltose (G2) 3. Maltotriose (G3) 4. Maltotetraose (G4) 5. Maltopentaose (G5) 6. Maltohexaose (G6) 7. Maltoheptaose (G7) 1. (Lactosyl fructoside) 1 2 3 4 5 6 7 0 10 20 30 min (A970703A) (F030620A) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (4.0 mg/mL) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (60/40) Flow rate : 1.0 mL/min Temperature : 25℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 10 μL (2.4 mg/mL) マルトオリゴ糖(G1 ∼G7) Maltooligosaccharides(G1 ∼ G7) マルトオリゴ糖(G1 ∼G7) Maltooligosaccharides(G1 ∼ G7) 1 1. Glucose (G1) 2. Maltose (G2) 3. Maltotriose (G3) 4. Maltotetraose (G4) 5. Maltopentaose (G5) 6. Maltohexaose (G6) 7. Maltoheptaose (G7) 2 1.Glucose(G1) 2.Maltose(G2) 3.Maltotriose (G3) 4.Maltotetraose (G4) 5.Maltopentaose (G5) 6.Maltohexaose (G6) 7.Maltoheptaose (G7) 3 4 5 6 7 0 5 10 15 20 25 min (F000612D) Column : Hydrosphere C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (26℃) Detection : RI, 64 x 10-6 RIU/FS Injection : 10 μL (5.0 mg/mL) 24 Courtesy of Dr.Kimura and Dr.Chiba, Hokkaido University, Japan Column : YMC-Pack ODS-AQ (5μm, 12nm) 250 × 10.0 mm I.D. Eluent : water Flow rate : 3.0 mL/min Temperature : ambient (25 ℃) Detection : RI (Y910501A) 糖類 2 Carbohydrates マルトオリゴ糖(G11 ∼G15) Maltooligosaccharides(G11 ∼ G15) マルトオリゴ糖(G11 ∼G15) Maltooligosaccharides(G11 ∼ G15) (T910718A) (M910604K) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (55/45) Flow rate : 1.0 mL/min Temperature : 26 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 200 μL (0.71∼0.95 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute Column マルトオリゴ糖(G11 ∼G15) Maltooligosaccharides(G11 ∼ G15) マルトオリゴ糖(G11 ∼G15) Maltooligosaccharides(G11 ∼ G15) : YMC-Pack ODS-A (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : 20 ℃ Detection : RI, 8 x 10-6 RIU/FS Injection : 25 μL (1 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute 1.Maltoundecaose (G11) 2.Maltododecaose (G12) 3.Maltotridecaose (G13) 4.Maltotetradecaose (G14) 5.Maltopentadecaose (G15) 1.Maltoundecaose (G11) 2.Maltododecaose (G12) 3.Maltotridecaose (G13) 4.Maltotetradecaose (G14) 5.Maltopentadecaose (G15) (M910604L) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : 20 ℃ Detection : RI, 8 x 10-6 RIU/FS Injection : 25 μL (1 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute 2 1.Maltoundecaose (G11) 2.Maltododecaose (G12) 3.Maltotridecaose (G13) 4.Maltotetradecaose (G14) 5.Maltopentadecaose (G15) Carbohydrates 1.Maltoundecaose (G11) 2.Maltododecaose (G12) 3.Maltotridecaose (G13) 4.Maltotetradecaose (G14) 5.Maltopentadecaose (G15) (M910604M) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (5/995) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : RI, 8 x 10-6 RIU/FS Injection : 25 μL (1 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute 25 糖類 2 Carbohydrates 2 マルトオリゴ糖(G11 ∼G15) Maltooligosaccharides(G11 ∼ G15) マルトオリゴ糖(G1 ∼G15) Maltooligosaccharides(G1 ∼ G15) 1.Maltoundecaose (G11) 2.Maltododecaose (G12) 3.Maltotridecaose (G13) 4.Maltotetradecaose (G14) 5.Maltopentadecaose (G15) 糖 類 1.Maltopentadecaose (G15) 2.Maltotridecaose (G13) 3.Maltoundecaose (G11) 4.Maltoheptaose (G7 ) 5.Maltopentaose (G5 ) 6.Maltotriose (G3 ) 7.Glucose (G1 ) (M910604N) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (5/995) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : RI, 8 x 10-6 RIU/FS Injection : 25 μL (1 mg/mL) Sample : Manufactured by Nakano Vinegar Co., Ltd., Nakano Central Research Institute : YMC-Pack Diol-60 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 64 x 10-6 RIU/FS Injection : 60 μL (0.60∼0.75 mg/mL) マルトオリゴ糖(G1 ∼G15) Maltooligosaccharides(G1 ∼ G15) サイクロデキストリン Cyclodextrins (G911216E) Column 1.Maltopentadecaose (G15) 2.Maltotridecaose (G13) 3.Maltoundecaose (G11) 4.Maltoheptaose (G7 ) 5.Maltopentaose (G5 ) 6.Maltotriose (G3 ) 7.Glucose (G1 ) 1. α-Cyclodextrin 2. β-Cyclodextrin 3. γ-Cyclodextrin 1 3 2 0 10 (G911216F) Column : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 64 x 10-6 RIU/FS Injection : 60 μL (0.60∼0.75 mg/mL) 26 20 min (F030617B) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (60/40) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 10 μL (0.4∼0.5 mg/mL) 糖類 2 Carbohydrates サイクロデキストリン Cyclodextrins サイクロデキストリン Cyclodextrins 2 1.γ-Cyclodextrin 2.α-Cyclodextrin 3.β-Cyclodextrin (M910417B) Carbohydrates 1.γ-Cyclodextrin 2.α-Cyclodextrin 3.β-Cyclodextrin (M910417A) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (7/93) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 8 μL (5∼15 mg/mL) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (7/93) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 8 μL (5∼15 mg/mL) サイクロデキストリン Cyclodextrins サイクロデキストリン Cyclodextrins 1.γ-Cyclodextrin 2.β-Cyclodextrin 3.α-Cyclodextrin 1.γ-Cyclodextrin 2.α-Cyclodextrin 3.β-Cyclodextrin 4.O-Dimethyl- β-cyclodextrin 5.O-Trimethyl-β-cyclodextrin (M910423A) Column (G911216I) Column : YMC-Pack Diol-60 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 30 μL (1.67 mg/mL) : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)water B)methanol 2%B (0-5min), 2-30%B (5-15min, linear), 30-100%B (15-20min, linear), 100%B (20-30min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection :Evaporative Mass Analyzer (ACS Ltd.) Temp 150 Time Constant 5 PMT 3 Attenuation 16 Pressure 20 psi Injection : 200 μL (1.5∼15 mg/mL) 27 糖類 2 Carbohydrates 2 サイクロデキストリン Cyclodextrins キチンオリゴ糖 N-Acetyl-chitooligosaccharides 1.O-Dimethyl- β-cyclodextrin 2.O-Trimethyl-β-cyclodextrin 糖 類 1. N-Acetylglucosamine 2. N-Acetyl-chitobiose 3. N-Acetyl-chitotriose 4. N-Acetyl-chitotetraose 5. N-Acetyl-chitopentaose 1 2 3 4 5 0 5 10 15 20 min (M910417C) (F030601A) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (80/20) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : RI, 32 x 10-6 RIU/FS Injection : 5 μL (15∼20 mg/mL) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : RI, 16 x 10-6 RIU/FS Injection : 15 μL (0.2 mg/mL) ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains 1.PA-Sugar Chain 014 1.PA-Sugar Chain 014 2.PA-Sugar Chain 001 2.PA-Sugar Chain 001 3.PA-Sugar Chain 002 3.PA-Sugar Chain 002 (G910607C) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : FLS at Ex.320 nm, Em. 400 nm, 8 mV/FS Injection : 2 μL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. 28 (G910607B) Column : YMC-Pack ODS-A (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : FLS at Ex.320 nm, Em. 400 nm, 16 mV/FS Injection : 2 μL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. 糖類 2 Carbohydrates ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains 2 1.PA-Sugar Chain 014 2.PA-Sugar Chain 001 2.PA-Sugar Chain 001 3.PA-Sugar Chain 002 3.PA-Sugar Chain 002 (G910607A) Carbohydrates 1.PA-Sugar Chain 014 (G910620B) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : FLS at Ex.320 nm, Em. 400 nm, 16 mV/FS Injection : 2 μL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. Column : YMC-Pack Polyamine II 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM NH4H2PO4 (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : FLS at Ex. 320 nm, Em. 400 nm, 8 mV/FS Injection : 5 μL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. ピリジルアミノ化(PA)糖 Pyridylamino(PA)-Sugar chains ポリデキストロース Polydextrose 1.Polydextrose II 1.PA-Sugar Chain 014 2.PA-Sugar Chain 001 3.PA-Sugar Chain 002 (G910620A) (G911226A) Column : YMC-Pack Polyamine II 150 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (80/20) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : FLS at Ex.320 nm, Em. 400 nm, 4 mV/FS Injection : 3 μL (3.3 pmol/mL) Sample : PA-Sugar Chain Series, manufactured by TAKARA SHUZO CO., LTD. Column : YMC-Pack Diol-60 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : RI, 64 x 10-6 RIU/FS Injection : 40 μL (25 mg/mL) 29 糖類 2 Carbohydrates 2 プルラン Pullulans プルラン Pullulans 1.Pullulan P-800 (MW 853,000) 2.Pullulan P-50 (MW 48,000) 3.Pullulan P-20 (MW 23,700) 4.Pullulan P-5 (MW 5,800) 1.Pullulan P-800 (MW 853,000) 2.Pullulan P-50 (MW 48,000) 3.Pullulan P-20 (MW 23,700) 4.Pullulan P-5 (MW 5,800) 糖 類 (G911216A) (G911216B) Column : YMC-Pack Diol-60 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 16 x 10-6 RIU/FS Injection : 180 μL (0.25 mg/mL) Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K. Column : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 16 x 10-6 RIU/FS Injection : 180 μL (0.25 mg/mL) Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K. プルラン Pullulans プルラン Pullulans 1.Pullulan P-800 (MW 853,000) 2.Pullulan P-50 (MW 48,000) 3.Pullulan P-20 (MW 23,700) 4.Pullulan P-5 (MW 5,800) 1.Pullulan P-800 (MW 853,000) 2.Pullulan P-50 (MW 48,000) 3.Pullulan P-20 (MW 23,700) 4.Pullulan P-5 (MW 5,800) (G911216C) Column : YMC-Pack Diol-200 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 16 x 10-6 RIU/FS Injection : 180 μL (0.25 mg/mL) Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K. 30 (G911216D) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 16 x 10-6 RIU/FS Injection : 180 μL (0.25 mg/mL) Sample : Shodex STANDARD P-82, manufactured by SHOWA DENKO K.K. 糖類 2 Carbohydrates コンドロイチン Chondroitin コンドロイチン硫酸 Ａ Chondroitin sulfate A 1.Chondroitin, Na salt 1.Chondroitin sulfate A, Na salt 2 Carbohydrates (G920107A) (G920107C) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 16 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) コンドロイチン硫酸 Ｂ Chondroitin sulfate B コンドロイチン硫酸 Ｃ Chondroitin sulfate C Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) Column 1.Chondroitin sulfate B, Na salt 1.Chondroitin sulfate C, Na salt (G920107E) (G920107D) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 16 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) 31 糖類 2 Carbohydrates 2 ヒアルロン酸 Hyaluronic acid ヘパリン Heparin 1.Hyaluronic acid, Na salt 1.Heparin, Na salt 糖 類 (G920107B) (G920107I) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 8 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) ヘパラン硫酸 Heparan sulfate ケラタン硫酸 Keratan sulfate 1.Heparan sulfate, Na salt 1.Keratan sulfate, Na salt (G920107F) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) 32 (G920107G) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) 糖類 2 Carbohydrates ケラタンポリ硫酸 Keratan polysulfate 2 1.Keratan polysulfate, Na salt Carbohydrates (G920107H) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : water Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : RI, 32 x 10-6 RIU/FS Injection : 20 μL (5.0 mg/mL) 33 核酸 3 Nucleic acids 核酸塩基・ヌクレオシド Nucleosides and bases 核酸塩基・ヌクレオシド Nucleosides and bases 1.Cytocine 2.Cytidine 3.Uracil 4.Deoxycytidine 5.Guanine 6.Adenine 7.Hypoxanthine 8.Uridine 9.Xanthine 10.Thymine 11.Inosine 12.Guanosine 13.Deoxyinosine 14.Deoxyguanosine 15.Xanthosine 16.Thymidine 17.Adenosine 18.Deoxyadenosine 3 核 酸 1.Cytocine 2.Cytidine 3.Uracil 4.Deoxycytidine 5.Guanine 6.Adenine 7.Hypoxanthine 8.Uridine 9.Xanthine 10.Thymine 11.Inosine 12.Guanosine 13.Deoxyinosine 14.Deoxyguanosine 15.Xanthosine 16.Thymidine 17.Adenosine 18.Deoxyadenosine (T910404C) (T910404A) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)20mM CH3COOH - CH3COONH4 (pH 3.5) B)20mM CH3COOH - CH3COONH4 (pH 3.5)/methanol (90/10) 30%B (0-5min), 30-100%B (5-13min,linear), 100%B (13-40min) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.08 AUFS Injection : 12 μL (0.001∼0.05 mg/mL) Column 核酸塩基 Nucleic acid bases 核酸塩基 Nucleic acid bases 3 1. 5 O HN H2N N N H 6. NH2 F N O N H 24 3. HN O Uracil 7 6 4. 2. O HN O N H CH3 1 Uracil O 3. HN NH Adenine H2N 4 O N NH N Guanine 5 F HN O H N N H N Cytosine NH2 N N O 3 2 O HN O N H 7. O NH Thymine 5-Fluorocytosine 1 NH2 N N Guanine Cytosine 2. 1. 5. NH2 N O : YMC-Pack ODS-AQ (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)20mM CH3COOH - CH3COONH4 (pH 3.5) B)20mM CH3COOH - CH3COONH4 (pH 3.5)/methanol (90/10) 30%B (0-5min), 30-100%B (5-13min,linear), 100%B (13-40min) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.08 AUFS Injection : 12 μL (0.001∼0.05 mg/mL) 4. O HN O N H N H 5-Fluorouracil CH3 Thymine 5. NH2 N N N NH Adenine 0 5 10 0 min 10 20 min (S991029A) (J010209E) Column : Hydrosphere C18 (3μm, 12nm) 100 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.128 AUFS Injection : 10 μL (0.01∼0.02 mg/mL) 34 Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.064 AUFS Injection : 8μL (0.01∼0.02 mg/mL) 核酸 3 Nucleic acids オロチン酸 Orotic acid ヌクレオシド Nucleosides NH2 1. N O N HOH2C O O 1. HN O N H O 2 N H 5 4. N H N HN N O 10 11. NH2 N N N N HOH2C O OH N N H N HOH2C O OH OH 5 OH OH Adenosine O 8. N OH OH Inosine 0 HOH2C O OH HOH2C O Orotic acid N Deoxyguanosine Deoxyadenosine O HN COOH N N O N HN H 2N N N HOH2C O Uridine HN 3 NH2 N Deoxyinosine OH OH O O 10. 7. O O N HOH2C O 8 2 3. N HOH2C O N OH HN 4 1 N Deoxycytidine 3. 3 HN 9 COOH 5-Fluoroorotic acid O 6. OH 7 OH Thymidine Nucleic acids F HN 3 NH2 O N HOH2C O 11 1 OH OH 6 O 2. O N HOH2C O Guanosine N CH3 HN OH OH 10 O 9. Cytidine 2. Uracil O N HN H 2N N N HOH2C O 5. Xanthosine min 0 5 10 15 min (A000802B) (A000711A) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM H3PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.256 AUFS Injection : 10μL (0.015∼0.1 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.5) / methanol (92/8) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 15 μL (5∼15 μg/mL) ヌクレオチド Nucleotides ヌクレオチド Nucleotides 1. 5'-CMP 2. 5'-UMP 3. 5'-dCMP 4. 5'-CDP 5. 5'-GMP 6. 5'-UDP 7. 5'-IMP 8. 5'-CTP 9. 5'-UTP 10. 5'-GDP 11. 5'-TMP 12. 5'-AMP 13. 5'-dGMP 14. 5'-GTP 15. 5'-TDP 16. 5'-ADP 17. 5'-TTP 18. 5'-dAMP 19. 5'-ATP 1. 5'-UTP 2. 5'-UDP 3. 5'-CMP 4. 5'-UMP 5. 5'-dCMP 6. 5'-GMP 7. 5'-ATP 8. 5'-IMP 9. 5'-ADP 10. 5'-TMP 11. 5'-AMP 5 1 7 2 4 8 10 6 3 11 9 0 5 10 15 20 min (T910424A) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluen : A) 0.2M TEAA* (pH6.6) B) 0.2M TEAA (pH6.6)/acetonitrile (95/5) 4%B (0-10min), 4-100%B (10-35min, linear), 100%B (35-50min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 14 μL (0.029∼0.16 mg/mL) * TEAA:triethylamine-acetic acid (A000922A) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM KH2PO4 - K2HPO4 (pH 5.5) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.256 AUFS Injection : 10 μL (0.05∼0.15 mg/mL) 35 核酸 3 Nucleic acids ヌクレオチド Nucleotides ヌクレオチド Nucleotides 1. 5'-CMP 2. 5'-UMP 3. 5'-GMP 4. 5'-IMP 5. 5'-TMP 6. 5'-AMP 5 2 4 3 1. 5'-CMP 2. 5'-AMP 3. 5'-UMP 4. 5'-IMP 5. 5'-GMP 6 3 1 核 酸 0 5 10 15 20 min (A000912B) (T920525C) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM KH2PO4 Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.256 AUFS Injection : 10μL (0.1∼0.3 mg/mL) : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : 50mM KH2PO4 - H3PO4 (pH 3.5) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.1 mg/mL) ヌクレオチド Nucleotides オリゴヌクレオチド Oligonucleotides Column 1. 5'-CMP 2. 5'-AMP 3. 5'-UMP 4. 5'-IMP 5. 5'-GMP 1.c-Ha-ras/12 (20mer) GTGGGCGCC-AGC-GGTGTGGG Ser 2.c-Ha-ras/12 (20mer) GTGGGCGCC-GCC-GGTGTGGG Ala 3.c-Ha-ras/12 (20mer) GTGGGCGCC-GAC-GGTGTGGG Asp 4.c-Ha-ras/12 (20mer) GTGGGCGCC-GTC-GGTGTGGG Val 5.c-Ha-ras/12 (20mer) GTGGGCGCC-CGC-GGTGTGGG Arg (G910620C) Column (T920525D) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 50mM KH2PO4 - H3PO4 (pH 3.5) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.1 mg/mL) 36 : YMC-Pack ODS-A (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : A)100mM triethylamine - acetic acid (pH 6.5)/acetonitrile (94/6) B)100mM triethylamine - acetic acid (pH 6.5)/acetonitrile (85/15) 0-100%B (0-25min,linear) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm,0.01 AUFS Injection : 15 μL (2.0 pmol/μL ) Sample : TAKARA ras Gene Probe set, manufacutured by TAKARA SHUZO CO., LTD. 核酸 3 Nucleic acids オリゴヌクレオチド，poly(A) Poly(A) 糖ヌクレオチド Sugar nucleotides 1.CDP-D-glucose 2.UDP-D-glucose 3.UDP-N-acetyl-D-glucosamine 4.GDP-D-mannose 5.ADP-D-glucose 3 Nucleic acids Courtesy of Dr.Y.Baba, Kobe Women's College of Pharmacy (Y920608A) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : A)0.1M phosophate buffer (pH 7) B)0.1M phosophate buffer (pH 7) / acetonitrile (75/25) 20-27%B (0-15min), 27-35%B (15-90min) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 260 nm Injection : 10 μL Sample : Poly(A) 糖ヌクレオチド Sugar nucleotides (T911206D) Column : YMC-Pack Ph (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 100mM TEAA1) (pH 6.0) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.27∼0.71 mg/mL) 1) TEAA: triethylamine-acetic acid 糖ヌクレオチド Sugar nucleotides 1.CDP-D-glucose 2.UDP-D-glucose 3.UDP-N-acetyl-D-glucosamine 4.GDP-D-mannose 5.ADP-D-glucose 1.CDP-D-glucose 2.UDP-D-glucose 3.UDP-N-acetyl-D-glucosamine 4.GDP-D-mannose 5.ADP-D-glucose (T911206B) (T911206E) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 20mM TEAA1) (pH 5.7)/acetonitrile (99/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.27∼0.71 mg/mL) Column : YMC-Pack ODS-A (5μm, 30nm) 250 × 4.6 mm I.D. Eluent : 100mM TEAA1) (pH 6.0) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.27∼0.71 mg/mL) 1) TEAA: triethylamine-acetic acid 1) TEAA: triethylamine-acetic acid 37 核酸 3 Nucleic acids プラスミドpBR322の制限酵素Msp I分解物 Plasmid pBR322 restriction fragments 1.Plasmid pBR322 cleaved with restriction endonuclease MspⅠ 3 核 酸 (G920109B) Column : YMC-Pack Diol-300 + Diol-200 500 × 8.0 mm I.D. × 2 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 260 nm, 0.01 AUFS Injection : 3 μL (0.49 mg/mL) Sample : Plasmid pBR322 cleaved with restriction endonuclease MspⅠ 38 アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins アミノ酸 Amino acids 市販ドリンク剤中のタウリン Taurine in nutritious drink 1. L-Valine 2. L-Tyrosine 3. L-Phenylalanine 4. L-Tryptophan (A) FLS 1. Taurine (B) UV 1 1 2 4 1 3 4 10 20 30 min 0 10 20 min 0 10 20 min (F001115C) (A000419B) : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 5 μL (0.05∼1.6 mg/mL) : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 10mM KH2PO4-K2HPO4(pH6.8)/acetonitrile (85/15) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : (A)FLS at Ex 345 nm, Em 450 nm (B)UV at 254 nm, 0.32 AUFS Injection : 15μL Sample : OPA derivative PTC-アミノ酸 PTC-amino acids PTH-アミノ酸 PTH-amino acids Column Column 1. L-Aspartic acid 2. L-Glutamic acid 3. L-Serine 4. Glycine 5. L-Histidine 6. L-Arginine 7. L-Threonine 8. L-Alanine 9. L-Proline 10. NH3 11. L-Tyrosine 12. L-Valine 13. L-Methionine 14. L-Cystine 15. L-Isoleucine 16. L-Leucine 17. L-Phenylalanine 18. L-Lysine 1. Asp 2. His 3. Arg 4. Asn 5. Glu 6. Ser 7. Gln 8. Thr 9. Gly 10. Ala 11. Tyr 12. Δ-Thr 13. Pro 14. Met 15. Val 16. Trp 17. Phe 18. Lys 19. Ile 20. Leu (G910523A) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A) 60mM CH3COONa - CH3COOH (pH 6.6)/acetonitrile (94/6) B) 60mM CH3COONa - CH3COOH (pH 6.6)/acetonitrile (40/60) 0-55%B (0-20min, linear), 100%B (20-25min), 0%B (25-35min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 250 nm, 0.08 AUFS Injection : 5 μL (62.5 pmoL/μL ) Sample : PTC derivatives (K940331A) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 2.0 mm I.D. Eluent : A)acetonitrile/THF/300mM TEAA* (pH 5.1) (10/4/90) B)acetonitrile/THF/300mM TEAA (pH 5.1) (75/4/25) 0-30%B (0-15min), 30-80%B (15-30min) Flow rate : 0.2 mL/min Temperature : 50 ℃ Detection : UV at 270 nm, 0.08 AUFS Injection : 30 μL (25pmol/10μL) *TEAA: triethylamine-acetic acid 39 Amino acids, Peptides, Proteins 0 アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins NBD-アミノ酸 NBD-amino acids 1. L-Aspartic acid 2. L-Glutamic acid 3. L-Asparagine 4. L-Serine 5. L-Histidine 6. Glycine 7. L-Arginine 8. L-Threonine 9. Taurine 10. L-Alanine 11. L-Proline 12. L-Valine 13. L-Cystine 14. L-Methionine 15. L-Isoleucine 16. L-Leucine 17. L-Phenylalanine 18. L-Lysine 19. L-Tyrosine 12 6 15 4 ア ミ ノ 酸 ・ ペ プ チ ド ・ タ ン パ ク 質 16 17 4 9 2 8 3 1 10 酸化型および還元型グルタチオン Glutathione, oxidized and reduced form 1.Glutathione (GSH) 2.Glutathione disulfide (GSSG) 19 7 11 5 18 13 14 0 10 20 30 40 50 60 min (F001129E) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)20mM KH2PO4-K2HPO4(pH6.5) B)20mM KH2PO4-K2HPO4(pH6.5)/acetonitrile (50/50) 15-25%B(0-25min), 25-40%B(25-30min), 40-65%B(30-50min),65-100%B(50-55min), 100%B(55-60min) Flow rate : 1.0 mL/min(0-25min), 1.5 mL/min(25-60min) Temperature : 30 ℃ Detection : FLS at Ex 470 nm, Em 530 nm Injection : 20 μL Sample : NBD derivatives ペプチドマップ Peptide mapping (H900710D) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 0.1M sodium perchlorate in A A)acetonitrile/water/TFA (5/95/0.05) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm,0.64 AUFS Injection : 10 μL ペプチドマップ Peptide mapping 39 50 Ser Glu Gln Asn Glu Glu Val Gly Ala Pro Leu Ser 1 16 17 25 Asn Pro Val Tyr Gly Ser Val Ser Asn Ala Asp Leu Met Asp Phe Lys Asn Leu Leu Asp His Leu Glu Asp Lys T1 T2 26 39 50 Met Pro Leu Glu Asp Glu Ala Met Pro Pro Gln Val Leu Ser Glu Gln Asn Glu Glu Val Gly Ala Pro Leu Ser T3 51 67 68 74 75 Pro Leu Leu Glu Val Pro Pro Trp Thr Gly Glu Val Asn Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly T4 76 82 83 87 88 89 90 91 92 98 99 Pro Trp Asp Ala Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu Ala Ala Pro Arg Ser Leu T5 T6 T7 T8 T9 T10 51 67 68 74 75 Pro Leu Leu Glu Val Pro Pro Trp Thr Gly Glu Val Asn Pro Ala Gln Arg Asp Gly Gly Ala Leu Gly Arg Gly T3' T4 82 83 87 88 89 90 91 92 98 99 76 Pro Trp Asp Ala Ser Asp Arg Ser Ala Leu Leu Lys Ser Lys Leu Arg Ala Leu Leu Ala Ala Pro Arg Ser Leu T5 T6 T7 T8 T9 T10 101 102 103 109 110 112 113 125 126 Arg Arg Ser Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr T14 T11 T15 T12 T13 101 102 103 109 110 112 113 125 126 Arg Arg Ser Ser Cys Phe Gly Gly Arg Met Asp Arg Ile Gly Ala Gln Ser Gly Leu Gly Cys Asn Ser Phe Arg Tyr T14 T11 T15 T12 T13 Courtesy of Dr.Nokihara, GBF-Gesellschaft für Biotechnologische Forschung Column Eluent Flow rate Detection Sample 40 : YMC-Pack ODS/R (5μm, 12nm) 250 × 4.6 mm I.D. : A)0.01N HCl B)acetonitrile 0%B (0min),0%B (10min),50%B (60min,linear) : 1.0 mL/min : UV 210 nm : Tryptic digests of synthetic pCDD 126 (Y890712B) Courtesy of Dr.Nokihara, GBF-Gesellschaft für Biotechnologische Forschung Column Eluent Flow rate Detection Sample : YMC-Pack ODS/R (5μm, 12nm) 250 × 4.6 mm I.D. : A)0.01N HCl B)acetonitrile 0%B (0min),0%B (10min),60%B (60min,linear) : 1.0 mL/min : UV 210 nm : Tryptic digests of synthetic pCDD 88 (Y890712A) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins アンジオテンシン Angiotensins アンジオテンシン Angiotensins 1.Angiotensin III 2.Angiotensin II 3.Angiotensin I 1.Angiotensin III 2.Angiotensin II 3.[Asn1,Val5] Angiotensin I 4.[Val5] Angiotensin I 5.Angiotensin I 4 (H900723B) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (20/80) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm,0.08 AUFS Injection : 8 μL (0.05∼0.15 mg/mL) Column : YMC-Pack PolymerC18 250 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (18/82/0.05) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm,0.08 AUFS Injection : 10 μL インスリン Insulins インスリン Insulins 1.Insulin (Bovine) 2.Insulin (Sheep) 3.Insulin (Human) 4.Insulin (Equine) 5.Insulin (Porcine) 1.Insulin (Bovine) 2.Insulin (Sheep) 3.Insulin (Human) 4.Insulin (Equine) 5.Insulin (Porcine) (G910522C) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/water/TFA (30/70/0.1) B)acetonitrile/water/TFA (32/68/0.1) 0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min) Flow rate : 0.6 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.16 AUFS Injection : 15 μL (0.2 mg/mL) (G910522B) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : A)acetonitrile/water/TFA (30/70/0.1) B)acetonitrile/water/TFA (32/68/0.1) 0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm,0.16 AUFS Injection : 15 μL (0.2 mg/mL) 41 Amino acids, Peptides, Proteins (G910520A) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins インスリン Insulins インスリン Insulins 1.Insulin (Bovine) 2.Insulin (Sheep) 3.Insulin (Human) 4.Insulin (Porcine) 1.Insulin (Bovine) 2.Insulin (Sheep) 3.Insulin (Human) 4.Insulin (Equine) 5.Insulin (Porcine) 4 ア ミ ノ 酸 ・ ペ プ チ ド ・ タ ン パ ク 質 (G910522A) (G910522D) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 6.0 mm I.D. Eluent : A)acetonitrile/water/TFA (30/70/0.1) B)acetonitrile/water/TFA (32/68/0.1) 0%B (0-3min), 0-100%B (3-25min,linear), 100%B (25-40min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm,0.16 AUFS Injection : 15 μL (0.2 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : A)acetonitrile/0.01N HCl (28/72) B)acetonitrile/0.01N HCl (35/65) 0-100%B (0-60min,linear) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm,0.16 AUFS Injection : 15 μL (0.25 mg/mL) ペプチド Peptides ペプチド Peptides 1. Bradykinin 2. Dynorphin A (Human,1-13) 3. Eledoisin related peptide 4. Neurotensin 5. Bombesin 6. Substance P 1. Oxytocin 2. Met-Enkephalin 3. Leu-Enkephalin 4. Angiotensin I 5. α-Mating factor 6. Insulin 2 5 3 4 4 5 1 6 1 6 2 3 0 5 10 15 0 20 min 5 10 15 (A980827C) (A980827D) Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 20-40%B (0-20min, linear) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 15 μL (0.05∼0.15 mg/mL) 42 20 min Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : A) water / TFA (100/0.1) B) acetonitrile / TFA (100/0.1) 20-40%B (0-20min, linear) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 15 μL (0.04∼0.15 mg/mL) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins ペプチド(MW 556 - 3, 465) Peptides(MW 556 - 3, 465) ペプチド Peptides 1. Oxytocin 2. Met-Enkephalin 3. Neurotensin 4. Leu-Enkephalin 5. γ-Endorphin 6. β-Endorphin(Human) 1. BAM-12P 2. [D-Ala2,Met5]-Enkephalinamide 3. α-Endorphin 4. Met-Enkephalin 5. [D-Ala2,Met5]-Enkephalin 6. γ-Endorphin 7. β-Endorphin(Human) (MW 1,007) (MW 574) (MW 1,673) (MW 556) (MW 1,859) (MW 3,465) 1 5 4 7 10 15 20 min (N041021C) (A000308B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) water / TFA (100/0.05) B) acetonitrile / TFA (100/0.05) 20-40%B (0-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm Injection : 10 μL (0.075∼0.25 mg/mL) Column ペプチド Peptides ペプチド - LC/MS Peptides - LC/MS 1. [Arg8]-Vasopressin 2. Oxytocin 3. Bradykinin 4. Angiotensin II 5. Leu-Enkephalin 6. Angiotensin I 5 4 : Hydrosphere C18 (5μm, 12nm) 150× 4.6 mm I.D. Eluent : A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 20-40%B(0-15 min, linear),40%B(15-20 min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.256 AUFS Injection : 10 μL (0.05∼0.1 mg/mL) 5 3 6 1. [Arg8]-Vasopressin 2. Bradykinin 3. Angiotensin II 4. Oxytocin 5. Angiotensin I 6. Leu-Enkephalin 4 A) UV 1 2 6 2 1 0.0 5.00 10.00 15.00 20.00 min 15.00 20.00 min 3 2 3 5 B) MS 1 0 5 10 15 6 4 min 0.0 5.00 10.00 (A020808A) (A010821A) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150× 4.6 mm I.D. Eluent : A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 15-35%B(0-15 min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 20 μL (0.05 mg/mL) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150× 2.0 mm I.D. Eluent : A) water/TFA (100/0.01) B) acetonitrile/TFA (100/0.01) 10-35%B(0-15 min),10%B(15-20 min) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : A) UV at 220 nm, B) ESI positive-mode Injection : 4 μL (0.05 mg/mL) 43 Amino acids, Peptides, Proteins 0 6 4 5 23 アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins ペプチド Peptides ペプチド Peptides 1.Met-Enkephalin 2.Bradykinin 3.Angiotensin II 4.Leu-Enkephalin 5.CCK-Octapeptide 6.Insulin Chain B 1. Bradykinin 2. Met-Enkephalin 3. Angiotensin III 4. Leu-Enkephalin 4 ア ミ ノ 酸 ・ ペ プ チ ド ・ タ ン パ ク 質 (S930909D) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/water/TFA (5/95/0.1) B)acetonitrile/water/TFA (40/60/0.1) 20-100%B (0-40min, linear) Flow rate : 1.5 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 40 μL (0.067∼0.13 mg/mL) : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (20/80/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 20 μL (34∼65 μg/mL) ペプチド Peptides ペプチド Peptides (O940804D) Column 1. Bradykinin 2. Met-Enkephalin 3. Angiotensin III 4. Leu-Enkephalin 1. Bradykinin 2. Met-Enkephalin 3. Angiotensin III 4. Leu-Enkephalin (O940804A) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 2.0 mm I.D. Eluent : acetonitrile/water/TFA (20/80/0.1) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.08 AUFS Injection : 4 μL (34∼65 μg/mL) 44 (O940804B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 1.5 mm I.D. Eluent : acetonitrile/water/TFA (20/80/0.1) Flow rate : 0.1 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.08 AUFS Injection : 2 μL (34∼65 μg/mL) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins ペプチド Peptides ペプチドおよびタンパク質(MW 4,330 - 17,000) Peptides and proteins(MW 4,330 - 17,000) 1. Bradykinin 2. Met-Enkephalin 3. Angiotensin III 4. Leu-Enkephalin 4 (O940804C) : J'sphere ODS-H80 (4μm, 8nm) 150 × 1.0 mm I.D. Eluent : acetonitrile/water/TFA (20/80/0.1) Flow rate : 0.05 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.08 AUFS Injection : 1 μL (34∼65 μg/mL) : YMC-Pack C8 (5μm, 20nm) 150 × 4.6 mm I.D. Eluent : A) water/TFA (100/0.1) B) acetonitrile/TFA (100/0.1) 25-60%B (0-20 min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm Injection : 10 μL (0.1∼0.2 mg/mL) ペプチドおよびタンパク質 Peptides and proteins タンパク質 Proteins Column Column 1.Ribonuclease A 2.Cytochrome c 3.Lysozyme 4.Myoglobin 1.Met-Enkephalin 2.Leu-Enkephalin 3.BSA 4.α-Mating factor 5.Insulin 6.Ovalbumin (H900606A) Column : YMC-Pack PolymerC18 150 × 4.6 mm I.D. Eluent : A)acetonitrile/water/TFA (20/80/0.05) B)acetonitrile/water/TFA (45/55/0.05) 0-100%B (0-30min,linear) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 30 μL (T931029A) Column : YMC-Pack ODS-A (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/water/TFA (5/95/0.1) B)acetonitrile/water/TFA (60/40/0.1) 30-90%B (0-20min, linear),90%B (20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 16 μL (0.16∼0.33 mg/mL) 45 Amino acids, Peptides, Proteins (N040820A) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins タンパク質 Proteins タンパク質(MW 18,300 - 45,000) Proteins(MW 18,300 - 45,000) 1.Ribonuclease A 2.Cytochrome c 3.Lysozyme 4.Myoglobin 1.β-Lactoglobulin B (Bovine milk) 2.β-Lactoglobulin A (Bovine milk) 3.α-Chymotrypsinogen A (Bovine pancreas) 4.Ovalbumin (MW 18,300) (MW 18,400) (MW 25,700) (MW 45,000) 4 ア ミ ノ 酸 ・ ペ プ チ ド ・ タ ン パ ク 質 (B931023A) (N041126A) Column : YMC-Pack C4 (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/water/TFA (5/95/0.1) B)acetonitrile/water/TFA (60/40/0.1) 30-90%B (0-20min, linear),90%B (20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 16 μL (0.16∼0.33 mg/mL) Column : YMC-Pack C4 (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : A)water/TFA (100/0.1) B)acetonitrile/TFA (100/0.1) 40-50%B (0-10min),50-90%B (10-15min),90%B (15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm Injection : 10 μL (0.2∼0.3 mg/mL) タンパク質(MW 66,000 - 96,000) Proteins(MW 66,000 - 96,000) ヒト免疫グロブリン Human immunoglobulins 1.Human IgM (κ),myeloma protein 2.Normal human IgA 3.Normal human IgG (N040930G) Column : YMC-Pack C4 (5μm, 30nm) 150 × 4.6 mm I.D. Eluent : A)water/TFA (100/0.1) B)acetonitrile/2-propanol/TFA (50/50/0.1) 30-75%B (0-15min),75%B (15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm Injection : 10 μL (0.25∼1.0 mg/mL) 46 (G920108G) Column : YMC-Pack Diol-300 + Diol-200 500 × 8.0 mm I.D. × 2 Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 0.7 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.04 AUFS Injection : 60 μL (0.33 mg/mL) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins ヒト免疫グロブリン Human immunoglobulins ヒト免疫グロブリン Human immunoglobulins 1.Human IgM (κ),myeloma protein 2.Normal human IgA 1.Human IgM (κ),myeloma protein 2.Normal human IgG 4 (G920108E) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.04 AUFS Injection : 40 μL (0.5 mg/mL) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.04 AUFS Injection : 40 μL (0.5 mg/mL) ヒト免疫グロブリン(IgM κ鎖, ミエローマタンパク質) Human IgM(κ),myeloma protein ヒト免疫グロブリン(IgA) Normal human IgA 1.Normal human IgM (κ),myeloma protein 1.Normal human IgA (G920108D) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.04 AUFS Injection : 20 μL (1.0 mg/mL) (G920108C) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.02 AUFS Injection : 20 μL (1.0 mg/mL) 47 Amino acids, Peptides, Proteins (G920108F) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins ヒト免疫グロブリン(IgG λ鎖, ミエローマタンパク質) Human IgG(λ),myeloma protein ヒト免疫グロブリン(IgG) Normal human IgG 1.Normal human IgG 1.Human IgG (λ),myeloma protein 4 ア ミ ノ 酸 ・ ペ プ チ ド ・ タ ン パ ク 質 (G920108B) (G920108A) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.04 AUFS Injection : 20 μL (1.0 mg/mL) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M NaH2PO4 - Na2HPO4 (pH 6.8) containing 0.1M Na2SO4 Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 280 nm, 0.04 AUFS Injection : 20 μL (1.0 mg/mL) チトクローム c Cytochrome c ミオグロビン分解物 Peptide fragments from myoglobin 1.Cytochrome c (hexamer) 2.Cytochrome c (tetramer) 3.Cytochrome c (trimer) 4.Cytochrome c (dimer) 5.Cytochrome c (monomer) 1. 1-153 (MW 17,000) 2. 1-131 (MW 14,500) 3. 56-153 (MW 10,700) 4. 56-131 (MW 8,200) 5. 1-55 (MW 6,300) 6. 132-153 (MW 2,500) (G911218A) Column (G911217D) Column : YMC-Pack Diol-200 + Diol-120 500 × 8.0 mm I.D. × 2 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 215 nm, 0.08 AUFS Injection : 30 μL (0.2 mg/mL) 48 : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) Flow rate : 0.7 mL/min Temperature : ambient (25 ℃) Detection : UV at 215 nm, 0.32 AUFS Injection : 20 μL (2.0 mg/mL) Sample : cyanogen bromide cleavages of horse heart myoglobim. Molecular Weight Marker for proteins,manufactured by Fluka Chemie AG. アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins ペプチド Peptides ペプチド Peptides 1.Insulin (Bovine) 2.Neurotensin 3.Angiotensin II 4.Glycine 1.Insulin (Bovine) 2.Neurotensin 3.Angiotensin II 4.Glycine 4 (G911217B) Column : YMC-Pack Diol-60 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) Flow rate : 0.7 mL/min Temperature : ambient (25 ℃) Detection : UV at 215 nm, 0.16 AUFS Injection : 25 μL (0.07∼5.3 mg/mL) Column : YMC-Pack Diol-120 500 × 8.0 mm I.D. × 2 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) Flow rate : 0.7 mL/min Temperature : ambient (25 ℃) Detection : UV at 215 nm, 0.16 AUFS Injection : 25 μL (0.07∼5.3 mg/mL) ペプチド Peptides グリシンオリゴマー Glycine oligomers 1.Insulin (Bovine) 2.Neurotensin 3.Angiotensin II 4.Glycine 1.Pentaglycine 2.Triglycine 3.Glycine (G911217C) Column : YMC-Pack Diol-120 + Diol-60 500 × 8.0 mm I.D. × 2 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (70/30) Flow rate : 0.7 mL/min Temperature : ambient (25 ℃) Detection : UV at 215 nm, 0.08 AUFS Injection : 25 μL (0.07∼5.3 mg/mL) (G911227B) Column : YMC-Pack Diol-60 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0)/acetonitrile (70/30) Flow rate : 1.0 mL/min Temperature : ambient (24 ℃) Detection : UV at 215 nm, 0.08 AUFS Injection : 20 μL (0.25∼2.5 mg/mL) 49 Amino acids, Peptides, Proteins (G911217A) アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins カゼイン Casein タンパク分子量マーカー Proteins for molecular weight marker 1.Casein 1.Glutamate dehydrogenase 2.Lactate dehydrogenase 3.Enolase 4.Adenylate kinase 5.Cytochrome c (MW 290,000) (MW 142,000) (MW 67,000) (MW 32,000) (MW 12,400) 4 ア ミ ノ 酸 ・ ペ プ チ ド ・ タ ン パ ク 質 (G911213A) (G920123B) : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl/acetonitrile (90/10) Flow rate : 1.0 mL/min Temperature : ambient (22 ℃) Detection : UV at 215 nm, 0.16 AUFS Injection : 15 μL (1.0 mg/mL) Column : YMC-Pack Diol-300 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 280 nm, 0.08 AUFS Injection : 15 μL (100 μL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. タンパク分子量マーカー Proteins for molecular weight marker タンパク分子量マーカー Proteins for molecular weight marker Column 1.Glutamate dehydrogenase 2.Lactate dehydrogenase 3.Enolase 4.Adenylate kinase 5.Cytochrome c (MW 290,000) (MW 142,000) (MW 67,000) (MW 32,000) (MW 12,400) 1.Glutamate dehydrogenase 2.Lactate dehydrogenase 3.Enolase 4.Adenylate kinase 5.Cytochrome c (G911214A) Column : YMC-Pack Diol-200 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 280 nm, 0.08 AUFS Injection : 15 μL (100 μL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. 50 (MW 290,000) (MW 142,000) (MW 67,000) (MW 32,000) (MW 12,400) (G911214I) Column : YMC-Pack Diol-200 300 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 280 nm, 0.16 AUFS Injection : 15 μL (100 μL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. アミノ酸、ペプチド、タンパク質 4 Amino acids, Peptides, Proteins タンパク分子量マーカー Proteins for molecular weight marker 1.Glutamate dehydrogenase 2.Lactate dehydrogenase 3.Enolase 4.Adenylate kinase 5.Cytochrome c タンパク分子量マーカー Proteins for molecular weight marker (MW 290,000) (MW 142,000) (MW 67,000) (MW 32,000) (MW 12,400) 1.Glutamate dehydrogenase 2.Lactate dehydrogenase 3.Enolase 4.Adenylate kinase 5.Cytochrome c (MW 290,000) (MW 142,000) (MW 67,000) (MW 32,000) (MW 12,400) 4 Column : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 280 nm, 0.08 AUFS Injection : 15 μL (100 μL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. (G911214C) Column : YMC-Pack Diol-120 + Diol-300 500 × 8.0 mm I.D. × 2 Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 280 nm, 0.08 AUFS Injection : 15 μL (100 μL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. タンパク分子量マーカー Proteins for molecular weight marker 1.Glutamate dehydrogenase 2.Lactate dehydrogenase 3.Enolase 4.Adenylate kinase 5.Cytochrome c (MW 290,000) (MW 142,000) (MW 67,000) (MW 32,000) (MW 12,400) (G911214H) Column : YMC-Pack Diol-200 + Diol-200 500 × 8.0 mm I.D. + 300 × 8.0 mm I.D. Eluent : 0.1M KH2PO4 - K2HPO4 (pH 7.0) containing 0.2M NaCl Flow rate : 0.7 mL/min Temperature : ambient (26 ℃) Detection : UV at 280 nm, 0.08 AUFS Injection : 15 μL (100 μL/1 vial) Sample : MW-Marker(HPLC), manufactured by ORIENTAL YEAST CO.,LTD. 51 Amino acids, Peptides, Proteins (G911214B) 有機酸、脂肪酸誘導体 5 Organic acids, Fatty acid derivatives 有機酸 Organic acids 有機酸 Organic acids 1. Glycolic acid 2. L-Malic acid 3. Malonic acid 4. Lactic acid 5. Acetic acid 6. Maleic acid 7. Citric acid 8. Fumaric acid 9. Succinic acid 10. Propionic acid 1. Tartaric acid 2. L-Malic acid 3. Lactic acid 4. Acetic acid 5. Citric acid 6. Succinic acid 1 6 24 3 5 5 0 有 機 酸 ・ 脂 肪 酸 誘 導 体 5 10 min (T910226A) (A001218A) Column : YMC-Pack ODS-AQ (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 20mM H3PO4 - NaH2PO4 (pH 2.8) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.08 AUFS Injection : 10 μL (0.007∼1.8 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NaH2PO4 - H3PO4(pH 2.8) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.064 AUFS Injection : 10 μL (0.25∼2.0 mg/mL) 有機酸 Organic acids ピルビン酸 Pyruvic acids 1. L-Malic acid 2. Lactic acid 3. Acetic acid 4. Maleic acid 5. Succinic acid 6. Propionic acid 7. Fumaric acid 1 Phosphoenolpyruvic acid monopotassium salt 2 Pyruvic acid (Y910209) Column : YMC-Pack PolymerC18 250 × 4.6 mm I.D. Eluent : 0.1% TFA Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.08 AUFS Injection : 10 μL (0.016∼2.2 mg/mL) 52 (O940901H) Column : YMC-Pack PolymerC18 250 × 4.6 mm I.D. Eluent : 100mM H3PO4 (pH 1.7) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.64 AUFS Injection : 5 μL (0.7, 20 mg/mL) 有機酸、脂肪酸誘導体 5 Organic acids, Fatty acid derivatives 不飽和脂肪酸メチルエステル Unsaturated fatty acid methyl esters リノレン酸メチル異性体 Methyl linolenate isomers 1.Eicosapentaenoic acid 1. Methyl linolenate 2. Methyl γ-linolenate 2.γ-Linolenic acid 3.Docosahexaenoic acid 4.Arachidonic acid 5.Linoleic acid 6.Eicosatrienoic acid 5 (K930323A) (F040713A) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (81/19) Flow rate : 1.5 mL/min Temperature : ambient Detection : UV at 190 nm, 0.128 AUFS Injection : 20 μL (0.165 mg/mL) Column : YMC-Pack Pro C18 RS (3μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/THF/water (35/30/35) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm Injection : 2 μL (2.5 μL/mL) 不飽和脂肪酸メチルエステル Unsaturated fatty acid methyl esters トリアシルグリセロール Triacylglycerols 1. LLL 2. LLO 3. POL 4. PPL 5. OOO 6. OOP 7. PPO 1.Methyl linolenate 2.Methylγ-linolenate 3.Methyl linoleate L : linoleic acid O: oleic acid P: palmitic acid 4.Methyl oleate (S931012C) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/THF/water (10/30/60) B)acetonitrile/THF/water (65/30/5) 20-100%B (0-40min, linear) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.51 AUFS Injection : 18 μL (0.25∼4 μL/mL) (O940914F) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/2-propanol (55/45) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.08 AUFS Injection : 30 μL (0.08∼1.66 mg/mL) 53 Organic acids, Fatty acid derivatives Courtesy of Dr. Yuji Saito, Tokyo Institute of Technology 食品添加物 6 Food additives 食品保存料 Food preservatives 食品保存料 Food preservatives 1 1 Dehydroacetic acid Saccharin sodium 2 2 Sorbic acid Dehydroacetic acid 3 3 Benzoic acid Sorbic acid 4 Benzoic acid 6 食 品 添 加 物 (O940819A) (O940802I) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/water/TFA (20/80/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 2 μL (0.13∼0.32 mg/mL) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/water/acetic acid (20/80/0.5) Flow rate : 2.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.08 AUFS Injection : 4 μL (56 μg/mL) 食品保存料 Food preservatives 食品保存料 Food preservatives COOH 1. 1. Benzoic acid Dehydroacetic acid 2. 2. CH3CH CHCH CHCOOH Sorbic acid Sorbic acid 1 3. 3. 2 Benzoic acid (S970204F) Column : YMC-Pack Pro C18 (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : THF/water/acetic acid (20/80/0.5) Flow rate : 2.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 5 μL (0.05 mg/mL) 54 5 10 O O 3 0 H3 C O COCH3 Dehydroacetic acid 15 min (J011213F) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM CH3COONa-CH3COOH(pH 4.3) / acetonitrile (80/20) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.26 AUFS Injection : 10 μL (0.02 mg/mL) 食品添加物 6 Food additives パラオキシ安息香酸エステル類 p-Hydroxybenzoic acid esters EDTAキレート EDTA-chelates 1. OH COOCH3 1 1 Methyl p-hydroxybenzoate 2. OH 2 COOCH2CH3 Ethyl p-hydroxybenzoate 3. OH 3 Fe (Ⅲ)-EDTA CH3 COOCH CH3 Isopropyl p-hydroxybenzoate 4 2 4. OH COO(CH2)2CH3 Propyl p-hydroxybenzoate 56 5. Cu (Ⅱ)-EDTA CH3 COOCH2CH CH3 EDTA: Ethylenediaminetetraacetic acid OH Isobutyl p-hydroxybenzoate 6. OH COO(CH2)3CH3 Butyl p-hydroxybenzoate 0 10 20 6 min (T940208A) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water (60/40) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.32 AUFS Injection : 4 μL (0.1 mg/mL) : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM CH3COONa - CH3COOH (pH 4.6) containing 20mM tetrabutylammonium bromide Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.04 AUFS Injection : 10 μL (0.017∼0.13 mg/mL) 酸化防止剤 Antioxidants 酸化防止剤 Antioxidants 1 Column n-Propyl gallate(PG) 2 tert-Butylhydroquinone (TBHQ) 2 6 n-Octyl gallate(OG) 4-Hydroxymethyl2,6-di-tert-butylphenol (HMBP) 6 n-Octyl gallate(OG) 2,4,5-Trihydroxy butyrophenone (THBP) 3 7 7 tert-Butylhydroquinone (TBHQ) n-Dodecyl gallate(DG) n-Dodecyl gallate(DG) 4 4 Nordihydroguaiaretic acid(NDGA) Nordihydroguaiaretic acid(NDGA) (A970524B) Column 5 n-Propyl gallate(PG) 4-Hydroxymethyl2,6-di-tert-butylphenol (HMBP) 2,4,5-Trihydroxy butyrophenone (THBP) 3 1 5 : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/methanol/5% acetic acid (20/20/60) B)acetonitrile/methanol/5% acetic acid (45/45/10) 0-100%B (0-10min), 100%B (10-20min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 9 μL (0.05∼0.6 mg/mL) (A970526C) Column : YMC-Pack Pro C18 (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : A)acetonitrile/methanol/5% acetic acid (20/20/60) B)acetonitrile/methanol/5% acetic acid (45/45/10) 0-100%B (0-5min), 100%B (5-15min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 9 μL (0.05∼0.6 mg/mL) 55 Food additives (J020826A) 食品添加物 6 Food additives 酸化防止剤 Antioxidants 酸化防止剤 Antioxidants 1 1 n-Propyl gallate(PG) n-Propyl gallate(PG) 2 2 2,4,5-Trihydroxybutyrophenone (THBP) 2,4,5-Trihydroxybutyrophenone (THBP) 3 3 tert-Butylhydroquinone(TBHQ) tert-Butylhydroquinone(TBHQ) 6 食 品 添 加 物 (A970522B) (A970522D) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/5% acetic acid (20/20/60) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 10 μL (0.05∼0.2 mg/mL) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/5% acetic acid (20/20/60) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 10 μL (0.05∼0.2 mg/mL) 酸化防止剤 Antioxidants 酸化防止剤 Antioxidants 1. CH3 CH3 CH2CH CHCH2 HO 1 HO Nordihydroguaiaretic acid(NDGA) 2. 24 5 HO HO OH Nordihydroguaiaretic acid (NDGA) 3 COOC8H17 HO n -Octyl gallate(OG) 5. OH C(CH3)3 6 OH 3(2)- tert -Butyl-4-hydroxyanisol (BHA) 3. 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP) CH3 2,6-Di- tert -butyl4-methylphenol (BHT) 6. OH (CH3)3C C(CH3)3 CH2OH 4-Hydroxymethyl-2,6di- tert -butylphenol (HMBP) C(CH3)3 (CH3)3C OCH3 1 2 4. OH HO HO COOC12H25 HO n -Dodecyl gallate(DG) 3 0 n-Octyl gallate(OG) 5 10 15 min (A970521B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/5% acetic acid (35/35/30) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 10 μL (0.1∼0.6 mg/mL) 56 (J010215B) Column : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : acetonitrile / methanol / 5% acetic acid (35/35/30) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 10 μL (0.05 ∼ 0.5 mg/mL) 食品添加物 6 Food additives 酸化防止剤 Antioxidants タール色素 Color additives 1 Butyl hydroxyanisol (BHA) 2 Dibutyl hydroxytoluene (BHT) 1. Tartrazine (FD&C Yellow No.5, C.I.19140, Y-4) 2. Amaranth (C.I.16185, R-2) 3. Indigo Carmine (FD&C Blue No.2, C.I.73015, B-2) 4. New Coccine (C.I.16255, R-102) 5. Sunset Yellow FCF (FD&C Yellow No.6, C.I.15985, Y-5) 6. Fast Green FCF (FD&C Green No.3, C.I.42053, G-3) 7. Brilliant Blue FCF (FD&C Blue No.1, C.I.42090, B-1) 8. Erythrosine (FD&C Red No.3, C.I.45430, R-3) 9. Acid Red (C.I.45100, R-106) 10. Phloxine (C.I.45410, R-104) 11. Rose Bengale (C.I.45440, R-105) 6 : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/water (95/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.16 AUFS Injection : 1 μL (0.5 mg/mL) : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 10mM NH4H2PO4 - (NH4)2HPO4 (pH 6.0)/methanol (90/10) B) 10mM NH4H2PO4 - (NH4)2HPO4 (pH 6.0)/methanol (20/80) 0-100%B (0-40min, linear), 100%B (40-60min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 100 μL (5 μg/mL) タール色素 Color additives タール色素 Color additives (O940810D) Column Column 1. Fast Green FCF (FD&C Green NO.3, C.I.42053, G-3) 2. Brilliant Blue FCF (FD&C Blue No.1, C.I.42090, B-1) 3. Erythrosine (FD&C Red No.3, C.I.45430, R-3) 4. Acid Red (C.I.45100, R-106) 5. Phloxine (C.I.45410, R-104) 6. Rose Bengale (C.I.45440, R-105) 1. Tartrazine (FD&C Yellow No.5, C.I.19140,Y-4) 2. Amaranth (C.I.16185, R-2) 3. Indigo Carmine (FD&C Blue No.2, C.I.73015, B-2) 4. New Coccine (C.I.16255, R-102) 5. Sunset Yellow FCF (FD&C Yellow No.6, C.I.15985, Y-5) (M910430C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM CH3COONH4 (25/75) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 10 μL (35∼140 μg/mL) (M910430A) Column :YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM CH3COONH4 (8/92) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (25∼200 μg/mL) 57 Food additives (M910427A) 食品添加物 6 Food additives 水溶性アナトー Annatto extract 市販炭酸飲料中のアスパルテーム Aspartame in a soft drink 1. Annatto pigment 1. Benzoic acid 2. Caffeine 3. Aspartame 6 (M910430E) 食 品 添 加 物 (T910123) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (65/34/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : VIS at 454 nm, 0.16 AUFS Injection : 5 μL (2 mg/mL) Sample : Annatto pigment : YMC-Pack Ph (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM KH2PO4/acetonitrile/methanol (84/3/13) Flow rate : 1.5 mL/min Temperature : 30 ℃ Detection : UV at 214 nm, 0.64 AUFS Injection : 10 μL 市販炭酸飲料中のアスパルテーム Aspartame in a soft drink 市販炭酸飲料中のアスパルテーム Aspartame in a soft drink Column 1.Caffeine 2.Aspartame 3.Benzoic acid 1.Caffeine 2.Aspartame 3.Benzoic acid (H900710A) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (15/85/0.05) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.64 AUFS Injection : 5 μL 58 (H900710B) Column : YMC-Pack PolymerC18 150 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (15/85/0.05) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.64 AUFS Injection : 5 μL 食品添加物 6 Food additives 市販炭酸飲料中のアスパルテーム Aspartame in a soft drink ステビオサイドとレバウディオサイドA Stevioside and rebaudioside A 1.Caffeine 2.Aspartame 3.Benzoic acid 1.Stevioside 2.Rebaudioside A 1 3 2 6 Column : YMC-Pack CN (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (15/85/0.05) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.64 AUFS Injection : 5 μL (G900727B) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water (35/65) Flow rate : 0.5 mL/min Temperature : 30 ℃ Detection : UV at 205 nm, 0.32 AUFS Injection : 7 μL グリチルリチン酸 Glycyrrhizic acid (F040622C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/5M acetic acid (400/560/40) Flow rate : 0.5 mL/min Temperature : 20 ℃ Detection : UV at 254 nm Injection : 20 μL (0.05∼0.25 mg/mL) （日本薬局方第14改正記載条件） 59 Food additives (H900710C) 天然物 7 Natural products アルカロイド Alkaloids アルカロイド Alkaloids 1.Hordenine sulfate 2.Pilocarpine chloride 3.Scopolamine bromide 4.Atropine sulfate 5.Cinchonine 6.Sparteine sulfate 7.Quinine 8.Papaverine chloride 9.Narcotine chloride 1.Hordenine sulfate 2.Pilocarpine chloride 3.Scopolamine bromide 4.Atropine sulfate 5.Cinchonine 6.Sparteine sulfate 7.Quinine 8.Papaverine chloride 9.Narcotine chloride (G910523K) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4(20/80) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 5 μL (0.05∼2.19 mg/mL) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : A)acetonitrile/20mM KH2PO4 (17/83) B)acetonitrile/20mM KH2PO4 (40/60) 0-100%B (0-30min, linear), 100%B (30-40min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 5 μL (0.03∼2.19 mg/mL) アルカロイド Alkaloids アルカロイド - 酢酸緩衝液 Alkaloids - acetate buffer (G910523J) 7 天 然 物 Column 1. 4 CH2 CH HO C H N H H 4. CH2 CH HO 2. CH2 CH HO 1 C H N H H 5. HO N 3. CH2 CH HO 6 CH3O N C H H N Quinidine 0 10 20 C H 2. 2 N H H HO C CH2 CH HO C 3. H 6 N H H CH3O N C H H Quinidine 0 10 20 N H H Quinine H N H H N N Dihydroquinine H N CH2 CH HO C CH3O 5. CH3CH2 HO C CH3O H N H H N Dihydroquinidine Cinchonidine 5 CH3CH2 CH3O 4. N N 6. N H H CH2 CH HO 1 Dihydroquinidine H C H N min : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/20mM KH2PO4 (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 235 nm, 0.08 AUFS Injection : 10 μL (0.01∼0.03 mg/mL) 60 HO 3 (J020828B) Column CH2 CH Cinchonine CH3CH2 CH3O Cinchonidine 5 N H H Quinine Cinchonine 2 1. 4 H N N 3 C CH3O Column H 6. CH3CH2 HO C CH3O H N H H N Dihydroquinine min (J021010D) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM CH3COOH-CH3COONH4(pH4.9)/methanol (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 235 nm, 0.08 AUFS Injection : 10 μL (0.01∼0.03 mg/mL) 天然物 7 Natural products プリンアルカロイド Purine alkaloids 生薬成分（配糖体） Crude drugs(Glycosides) 1. Trigonerlline 2. Theobromine 3. Theophylline 4. Caffeine 5. Phenol 1.Arbutin 2.Salicin 3.Esculine 4.G-Strophanthin (Ouabain) 5.Baicalin 6.Barbaloin 7.Glycyrrhizin 8.Digitoxin (G910618B) 生薬成分（配糖体） Crude drugs(Glycosides) 生薬成分（配糖体） Crude drugs(Glycosides) Column Column 1.Arbutin 2.Salicin 3.Esculine 4.G-Strophanthin (Ouabain) 5.Baicalin 6.Barbaloin 7.Glycyrrhizin 8.Digitoxin 1.Arbutin 2.Salicin 3.Esculine 4.G-Strophanthin (Ouabain) 5.Baicalin 6.Barbaloin 7.Glycyrrhizin 8.Digitoxin (G910607D) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)methanol/20mM NH4H2PO4 (5/95) B)methanol/20mM NH4H2PO4 (80/20) 0-100%B (0-20min, linear), 100%B (20-40min) Flow rate : 0.6 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.08 AUFS Injection : 18 μL (0.01∼0.49 mg/mL) 7 (G910607E) Column : YMC-Pack ODS-AQ (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)methanol/20mM NH4H2PO4 (5/95) B)methanol/20mM NH4H2PO4 (80/20) 0-100%B (0-20min, linear), 100%B (20-40min) Flow rate : 0.6 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.08 AUFS Injection : 18 μL (0.01∼0.49 mg/mL) 61 Natural Products : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (8/92) Flow rate : 1.0 mL/min Temperature : 20 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 5 μL (0.3∼4.5 mg/mL) : YMC-Pack ODS-A (5μm, 30nm) 250 × 6.0 mm I.D. Eluent : A)methanol/20mM NH4H2PO4 (5/95) B)methanol/20mM NH4H2PO4 (80/20) 0-100%B (0-20min, linear), 100%B (20-40min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.04 AUFS Injection : 20 μL (0.01∼0.49 mg/mL) (M910612C) 天然物 7 Natural products ペオニフロリン Paeoniflorin ペオニフロリン Paeoniflorin 1 1 Paeoniflorin 2 1. Albiflorin 2. Paeoniflorin 3. p-Hydroxyacetophenone(I.S.) 2 3 0 5 10 p-Hydroxyacetophenone(I.S.) 15 20 min (A980818A) 7 天 然 物 (O941121M) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (20/80/1) Flow rate : 0.4 mL/min Temperature : 25 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 10 μL (48∼56 μg/mL) : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM (NH4)2HPO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 3 μL (0.67, 0.33 mg/mL) 当帰芍薬散中のペオニフロリン Paeoniflorin in Toki-shakuyaku-san トリテルペン系サポニン Saponins from roots of rubus suavissimus S. Lee Column 1 1 Paeoniflorin 2 Courtesy of Dr.Ohtani,Hiroshima University,Japan Column (O941121I) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : THF/50mM (NH4)2HPO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 3 μL Sample : water extract from Toki-shakuyaku-san 62 (Y910123) : YMC-Pack PolymerC18 and YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (45/55) for PolymerC18 methanol/water (50/50) for ODS-AM Flow rate : 0.8 mL/min Temperature : 50 ℃ Detection : UV at 210 nm, 0.32 AUFS Sample : 1) β-D-Glucopyranosyl 2α,3β,23-trihydroxyurs12,18-dien-28-oate 2) β-D-Glucopyranosyl 2α,3β,23-trihydroxyurs12,19-dine-28-oate 天然物 7 Natural products サイコサポニン Saikosaponins サイコサポニン Saikosaponins 1 1 Saikosaponin c Saikosaponin c 2 2 Saikosaponin a Saikosaponin a 3 3 Saikosaponin b2 Saikosaponin b2 4 4 Saikosaponin d Saikosaponin d (S931210C) (S931210D) : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (38/62) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.26 AUFS Injection : 8 μL (0.5∼0.75 mg/mL) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (38/62) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.26 AUFS Injection : 8 μL (0.5∼0.75 mg/mL) ジンセノサイド Ginsenosides ジンセノサイド Ginsenosides 7 Natural Products Column 1 1 Ginsenoside-Rg1 Ginsenoside-Rg1 2 2 Ginsenoside-Rb1 Ginsenoside-Rb1 (A971118B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 203 nm, 0.16 AUFS Injection : 10 μL (0.125, 0.5 mg/mL) (A971120C) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 203 nm, 0.16 AUFS Injection : 10 μL (0.125, 0.5 mg/mL) 63 天然物 7 Natural products ジンセノサイド Ginsenosides フラボノイド Flavonoids 1 1 Myricetin 2 Ginsenoside-Rg1 Quercetin 2 3 Kaempferol Ginsenoside-Rb1 (A971121D) Column 7 天 然 物 : YMC-Pack Pro C18 (5μm, 12nm) 150 × 3.0 mm I.D. Eluent : A)acetonitrile/water (20/80) B)acetonitrile/water (40/60) 0-100%B (0-10min), 100%B (10-20min) Flow rate : 0.4 mL/min Temperature : 37 ℃ Detection : UV at 203 nm, 0.32 AUFS Injection : 4 μL (0.125, 0.5 mg/mL) フラボノイド Flavonoids (D960722A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 2 μL (0.05 mg/mL) フラボノイド Flavonoids 1 2 1 Luteolin 2 Apigenin 3 3 Chrysoeriol 4 Baicalein 5 5 Chrysin 6 Acacetin Acacetin (S961107C) 64 Baicalein Chrysin 6 : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 5 μL (0.05 mg/mL) Apigenin Chrysoeriol 4 Column Luteolin (S961111B) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 5 μL (0.05 mg/mL) 天然物 7 Natural products クエルセチンとその配糖体 Quercetin and its glycosides バイカリン Baicalin 1 HO HO O OH OR OH O Baicalin 2 1 1. Rutin R=rutinose 2. Isoquercitrin R=glucose 3. Quercitrin R=rhamnose 4. Quercetin R=H 2 3 Methyl p-hydroxybenzoate 4 0 5 10 15 min (J020828F) Column Column : YMC-Pack Pro C18 (5μm, 12nm) 50 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (30/70) Flow rate : 0.7 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.064 AUFS Injection : 10 μL (0.01, 0.02 mg/mL) 7 Natural Products : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (25/75) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.05 mg/mL) (S961105C) （日本薬局方第13改正参照） フラバノン配糖体 Flavanone glycosides フラバノン配糖体 Flavanone glycosides 1. 1. Narirutin Narirutin 2. 2. Naringin Naringin 3. 3. Hesperidin Hesperidin 4. 4. Neohesperidin Neohesperidin (A970430A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (20/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.04 AUFS Injection : 7 μL (0.025 mg/mL) (A970506A) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM H3PO4 (20/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.04 AUFS Injection : 7 μL (0.025 mg/mL) 65 天然物 7 Natural products 市販みそ中のイソフラボン Isoflavones from miso,fermented soybean paste カテキン類 Catechins OH 1 1 OH HO HOH2C HO O O O OH OH (-)-Epigallocatechin O HO OH Daidzin 1 OH 2 4 OH HO O 2 HOH2C 2 HO OH OH O OH O O OH O (+)-Catechin OH OH O HO 1 OH 3 2 Genistin 4 OH 3 OH HO 5 O OH 3 3 OH HO (-)-Epicatechin O OH O 4 OH OH HO O Daidzein 4 HO OH O OH OH CO OH OH O (-)-Epigallocatechin gallate OH O OH OH 5 Genistein OH HO O O 0 10 20 30 40 OH min 0 10 20 min OH OH (F001025B) (-)-Epicatechin gallate Column 7 天 然 物 : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)water/acetic acid (100/1) B)acetonitrile/water/acetic acid (50/50/1) 30%B(0-5min), 30-70%B(5-35min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08AUFS Injection : 7 μL Sample : methanol extract of miso OH CO (F000828A) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/ethyl acetate/water/TFA (9/3/88/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 7 μL (0.2∼0.8 mg/mL) カテキン類 Catechins カテキン類 Catechins 1 1 (-)-Epigallocatechin (-)-Epigallocatechin 2 2 (+)-Catechin (+)-Catechin 3 3 (-)-Epigallocatehin gallate (-)-Epigallocatehin gallate 4 4 (-)-Epicatechin (-)-Epicatechin 5 5 (-)-Epicatechin gallate (-)-Epicatechin gallate (S940124A) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - H3PO4 (pH 2.4)/methanol (75/25) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 8 μL (0.1∼0.4 mg/mL) 66 (S940122D) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - H3PO4 (pH 2.4)/methanol (75/25) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 8 μL (0.1∼0.4 mg/mL) 天然物 7 Natural products 2-フェニルエチルグリコシド 2-Phenylethyl glycosides 1 センノシド Sennosides HO OH O HO OH O HO HO O O OH 2-Phenylethyl β-melibioside 2 1 HO O HO HO O OH HO HO O O OH 2-Phenylethyl β-gentiobioside 3 HO OH HO O O O HO OH HO O OH 2-Phenylethyl β-lactoside 2 1 4 HO HO O HO HO 2 O O OH 3 Sennoside B HO O OH 2-Phenylethyl β-cellobioside 4 5 5 HO HO HO O OH HO O O HO O OH 2-Phenylethyl β-maltoside Sennoside A 0 10 20 30 40 50 min (F000911A) (A961126C) Courtesy of Dr.K.Sakata, Institute for Chemical Research Kyoto University, Japan Column : Hydrosphere C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/water (8/4/88) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 5 μL (0.2 mg/mL) Column センノシド Sennosides センノシド Sennosides : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (30/65/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 340 nm, 0.16 AUFS Injection : 10 μL (0.1 mg/mL) Natural Products 1 1 Sennoside B Sennoside B 2 2 Sennoside A Sennoside A (A961126B) Column 7 : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (30/65/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 340 nm, 0.16 AUFS Injection : 10 μL (0.1 mg/mL) (A961126D) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (30/65/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 340 nm, 0.16 AUFS Injection : 10 μL (0.1 mg/mL) 67 天然物 7 Natural products アルブチンおよび関連化合物 Arbutin and related compounds アルブチンおよび関連化合物 Arbutin and related compounds 1 1 Arbutin Arbutin 2 2 Hydroquinone Hydroquinone 3 3 Gallic acid Gallic acid (A970514D) 7 天 然 物 (A970514B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM H3PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 5 μL (0.2∼0.5 mg/mL) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM H3PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 5 μL (0.2∼0.5 mg/mL) アルブチンおよび関連化合物 Arbutin and related compounds ペオノール Paeonol OH 1. 1 3 O glucose Arbutin Paeonol 2 2 2. OH Butyl p-hydroxybenzoate OH 1 Hydroquinone 3. COOH OH HO OH Gallic acid 0 5 10 min (S971106D) (A980831L) Column Column : YMC-PacK Pro C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : methanol / water / TFA (5/95/0.05) Flow rate : 0.2 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.16 AUFS Injection : 1 μL (0.2∼0.5 mg/mL) 68 : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (35/65/2) Flow rate : 1.0 mL/min Temperature : ambient (22 ℃) Detection : UV at 274 nm, 0.26 AUFS Injection : 10 μL (0.04, 0.2 mg/mL) （日本薬局方第13改正記載条件） 天然物 7 Natural products ペオノール Paeonol アフラトキシン Aflatoxins 1 1 Aflatoxin G2 2 Paeonol 2 Aflatoxin G1 3 Butyl p-hydroxybenzoate Aflatoxin B2 4 Aflatoxin B1 (S971107A) (K931124B) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (35/65/2) Flow rate : 1.0 mL/min Temperature : ambient (22 ℃) Detection : UV at 274 nm, 0.26 AUFS Injection : 10 μL (0.04, 0.2 mg/mL) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water (40/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 365 nm, 0.04 AUFS Injection : 10 μL (7∼10 ppm) アフラトキシン Aflatoxins Natural Products （日本薬局方第13改正記載条件） 7 クロロフィル Chlorophyll a and b 1 1 Aflatoxin G2 2 Chlorophyll b Aflatoxin G1 3 2 Aflatoxin B2 4 Aflatoxin B1 Chlorophyll a Courtesy of Dr. Toyoaki Aoki, University of Osaka Pref. (K940228A) (K931125A) Column Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water (40/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 365 nm, 0.04 AUFS Injection : 10 μL (7∼10 ppm) : YMC-Pack ODS-A (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : methanol Flow rate : 1.0 mL/min Temperature : ambient Detection : VIS at 450 nm, 1 AUFS Injection : 100 μL Sample : extract of Spinacia with acetone/water (90/10) 69 天然物 7 Natural products トウガラシ中のカプサイシン類 Capsaicinoids from red pepper トウガラシ中のカプサイシン類 - LC/MS Capsaicinoids from red pepper - LC/MS 1. O mAU H3CO N H HO Nordihydrocapsaicin 70 2. O 60 H3CO N H HO Capsaicin 50 3. 40 O H3CO N H HO 2 30 Dihydrocapsaicin 20 3 10 1 0 0 2 4 6 8 10 min (J040910A) (F040728C) Column Column 7 天 然 物 : YMC-Pack Pro C18 RS (3μm, 8nm) 50 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (60/40/1) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 280 nm Injection : 1 μL (1 g/3 mL) Sample : methanol extract of a commercial cayenne pepper 70 : YMC-Pack Pro C18 RS (3μm, 8nm) 50 × 2.0 mm I.D. Eluent : methanol/water/acetic acid (60/40/0.1) Flow rate : 0.2 mL/min Temperature : 40 ℃ Detection : A) UV at 280 nm B) ESI positive-mode Injection : 1 μL (1 g/3 mL) Sample : methanol extract of a commercial cayenne pepper ステロイド 8 Steroids ステロイド Steroids ステロイド Steroids 1 1 Progesterone Hydrocortisone 2 2 Corticosterone Deoxycorticosterone 3 3 Deoxycorticosterone Corticosterone 4 4 Progesterone Hydrocortisone (S930322B) Column : YMC-Pack CN (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (40/60) Flow rate : 0.6 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 3 μL (0.25 mg/mL) (S930319D) Column : YMC-Pack CN (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : hexane/methanol/dichloromethane (75/20/5) Flow rate : 0.6 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 3 μL (0.25 mg/mL) 8 副腎皮質ステロイド Adrenocorticosteroids 1 1 Steroids 副腎皮質ステロイド Adrenocorticosteroids 6 4 Prednisolone 2 Hydrocortisone Prednisone 2 Hydrocortisone acetate 7. 5 Hydrocortisone 3 Corticosterone Cortisone 3 Cortisone acetate 8 6 Cortisone 4 Prednisolone Deoxycorticosterone 9 Methylprednisolone Corticosterone 5 Corticosterone acetate 10 Benzene Fluocinolone acetonide (S940308B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : THF/water (20/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 8 μL (0.1 mg/mL) (S940222C) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 7 μL (0.07 mg/mL) 71 ステロイド 8 Steroids 副腎皮質ステロイド Adrenocorticosteroids 副腎皮質ステロイド Adrenocorticosteroids 1 5 5 1 Hydrocortisone Hydrocortisone Dexamethasone 2 Dexamethasone 6 6 2 Cortisone Cortisone Corticosterone Corticosterone 3 7 7 3 Methylprednisolone 4 Beclomethasone Methylprednisolone 8 4 Beclomethasone 8 Triamcinolone acetonide Betamethasone Triamcinolone acetonide Betamethasone (S940310E) (S940310F) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (27/73) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 10 μL (0.08 mg/mL) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (27/73) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 10 μL (0.08 mg/mL) ヒドロコルチゾンとプレドニゾン Hydrocortisone and prednisone 副腎皮質ステロイド Adrenocorticosteroids 8 ス テ ロ イ ド 1 1 Fluocinolone acetonide 2 Prednisone 2 Prednisolone acetate 3 Hydrocortisone Deoxycorticosterone 4 Fluocinonide (K940530C) Column : YMC-Pack SIL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : chloroform/2-propanol/acetic acid (95/5/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.32 AUFS Injection : 10 μL (0.25 mg/mL) 72 (O940408A) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 5 μL (0.1 mg/mL) ステロイド 8 Steroids 副腎皮質ステロイド Adrenocorticosteroids 副腎皮質ステロイド Adrenocorticosteroids 1 1 Triamcinolone Fluocinolone acetonide 2 2 Prednisolone Prednisolone acetate 3 3 Triamcinolone acetonide Deoxycorticosterone 4 Fluocinonide (O940408C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 5 μL (0.1 mg/mL) (S940420B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 4 μL (0.1∼0.2 mg/mL) 8 副腎皮質ステロイド Adrenocorticosteroids 1 Steroids 副腎皮質ステロイド Adrenocorticosteroids 1 Triamcinolone Prednisolone succinate 2 2 Prednisolone Hydrocortisone succinate 3 Triamcinolone acetonide (S940420C) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 4 μL (0.1∼0.2 mg/mL) (S940414A) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (30/70/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 5 μL (0.1 mg/mL) 73 ステロイド 8 Steroids 副腎皮質ステロイド Adrenocorticosteroids 副腎皮質ステロイド Adrenocorticosteroids 1. Prednisone 2. Cortisone 3. Prednisolone 4. Hydrocortisone 5. Methylprednisolone 6. Corticosterone 7. Betamethasone 8. Dexamethasone 9. Triamcinolone acetonide 10. Beclomethasone 11. Fluocinolone acetonide 12. Prednisolone acetate 13. Hydrocortisone acetate 14. Cortisone acetate 15. Deoxycorticosterone 16. Corticosterone acetate 17. Fluocinonide 18. Betamethasone valerate 19. Deoxycorticosterone acetate 20. Beclomethasone dipropionate 21. Ethylbenzene 1. Prednisone 2. Cortisone 3. Prednisolone 4. Hydrocortisone 5. Methylprednisolone 6. Corticosterone 7. Betamethasone 8. Dexamethasone 9. Triamcinolone acetonide 10. Beclomethasone 11. Fluocinolone acetonide 12. Prednisolone acetate 13. Hydrocortisone acetate 14. Cortisone acetate 15. Deoxycorticosterone 16. Corticosterone acetate 17. Fluocinonide 18. Betamethasone valerate 19. Deoxycorticosterone acetate 20. Beclomethasone dipropionate 21. Ethylbenzene (S940303D) (S940303B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/THF/water (15/10/75) B)acetonitrile/water (75/25) 0-50%B (0-30min, linear), 50-100%B (30-50min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 10 μL (0.045 mg/mL) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/THF/water (15/10/75) B)acetonitrile/water (75/25) 0-50%B (0-30min, linear), 50-100%B (30-50min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 10 μL (0.045 mg/mL) 副腎皮質ステロイド Adrenocorticosteroids ステロイド Steroids 8 ス テ ロ イ ド 1. Prednisone 2. Cortisone 3. Prednisolone 4. Hydrocortisone 5. Methylprednisolone 6. Corticosterone 7. Betamethasone 8. Dexamethasone 9. Triamcinolone acetonide 10. Beclomethasone 11. Fluocinolone acetonide 12. Prednisolone acetate 13. Hydrocortisone acetate 14. Cortisone acetate 15. Deoxycorticosterone 16. Corticosterone acetate 17. Fluocinonide 18. Betamethasone valerate 19. Deoxycorticosterone acetate 20. Beclomethasone dipropionate 21. Ethylbenzene 1 Fluoxymesterone 2 Methylprednisolone 3 Prednisolone (S940303C) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/THF/water (15/10/75) B)acetonitrile/water (75/25) 0-50%B (0-30min, linear), 50-100%B (30-50min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 10 μL (0.045 mg/mL) 74 (K940602B) Column : YMC-Pack SIL-06 (5μm, 6nm) 250 × 4.6 mm I.D. Eluent : hexane/THF/acetic acid (60/40/1) Flow rate : 1.5 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.16 AUFS Injection : 10 μL (0.25 mg/mL) ステロイド 8 Steroids 男性ホルモン Androgens 男性ホルモン Androgens 1 4 1 Fluoxymesterone Methyltestosterone Fluoxymesterone 2 2 5 5 trans -Androsterone 3 3 Methyltestosterone Testosterone trans -Androsterone Testosterone 4 6 6 Dehydroisoandrosterone Dehydroisoandrosterone Androsterone Androsterone (S930707D) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/acetonitrile/water (45/15/40) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 10 μL (0.1∼2.0 mg/mL) (S930707C) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/acetonitrile/water (45/15/40) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 10 μL (0.1∼2.0 mg/mL) 8 テストステロン誘導体 Synthetic androgens 1 Steroids 男性ホルモン Androgens 4 1 Fluoxymesterone 2 Methyltestosterone 5 Testosterone 2 Testosterone 3 trans -Androsterone 6 Testosterone propionate 3 Dehydroisoandrosterone Androsterone Testosterone enanthate (S930706G) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/acetonitrile/water (45/15/40) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 10 μL (0.1∼2.0 mg/mL) (S940412B) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 5 μL (0.1∼0.25 mg/mL) 75 ステロイド 8 Steroids テストステロン誘導体 Synthetic androgens 女性ホルモン Estrogens and progestins 1 1 5 Estriol Testosterone Estrone 2 6 2 β-Estradiol Testosterone propionate Progesterone 3 7 3 Norethisterone 4 Testosterone enanthate Medroxyprogesterone acetate 8 Ethinylestradiol Mestranol (S940412C) (S930311D) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (70/30) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 5 μL (0.1∼0.25 mg/mL) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/THF/water (46/4/50) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 7 μL (0.1 mg/mL) 女性ホルモン Estrogens and progestins エストラジオール誘導体 Estrogens 8 ス テ ロ イ ド 1 5 1 Estriol Estrone 2 6 β-Estradiol 2 β-Estradiol Progesterone 3 7 Norethisterone Medroxyprogesterone acetate Estradiol benzoate 3 4 8 Ethinylestradiol Estradiol dipropionate Mestranol (S930312C) Column : YMC-Pack C8 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/THF/water (46/4/50) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 7 μL (0.1 mg/mL) 76 (S940428E) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 6 μL (0.08∼0.5 mg/mL) ステロイド 8 Steroids エストラジオール誘導体 Estrogens ノルゲストレルとエチニルエストラジオール Norgestrel and ethinylestradiol 1. 1. β-Estradiol Ethinylestradiol 2. 2. Norgestrel Estradiol benzoate 3. 3. Diphenyl(I.S.) Estradiol dipropionate (S940428F) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 6 μL (0.08∼0.5 mg/mL) (S940427B) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (50/50) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 6 μL (0.05∼0.1 mg/mL) 8 トンタン（豚胆）中の胆汁酸のフェナシルエステル誘導体 Phenacyl esters of bile acids from pig gall 1.Cholic acid (CA) 2.Hyodeoxycholic acid (HDCA) 3.Chenodeoxycholic acid (CDAC) 1. Ethinylestradiol 2. Norgestrel 3. Diphenyl(I.S.) (G900417B) (S940426A) Column Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (50/50) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 6 μL (0.05∼0.1 mg/mL) : YMC-Pack ODS-AL (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (60/40) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 240 nm, 0.04 AUFS Sample : extract of pig gall were derivatized with phenacyl bromide Injection : 5 μL 77 Steroids ノルゲストレルとエチニルエストラジオール Norgestrel and ethinylestradiol ステロイド 8 Steroids エクダイソン Ecdysones ステロール Sterols 1.β-Ecdysone 2.α-Ecdysone 4 1 Brassicasterol Stigmasterol 5 2 Cholesterol Campesterol 6 3 Fucosterol β-Sitosterol (O940701C) Courtesy of Dr.Furusawa, Kyoto Institute of Technology, Japan (G910523H) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/water(5/50/45) Flow rate : 0.5 mL/min Temperature : ambient Detection : UV at 254 nm, 0.04 AUFS Injection : 5 μL (0.04 mg/mL) Column : YMC-Pack ODS-AL (5μm, 12nm) + ODS-AL + ODS-AL + J'sphere ODS-M80 (4μm, 8nm) (250 × 4.6 mm I.D.) x 4 Eluent : methanol/water (100/2) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 10 μL (0.4∼1 mg/mL) ステロール Sterols ステロール Sterols Column 8 ス テ ロ イ ド 1 1 Brassicasterol Brassicasterol 2 2 Cholesterol Cholesterol 3 3 Fucosterol Fucosterol 4 4 Stigmasterol Stigmasterol 5 5 Campesterol Campesterol 6 6 β-Sitosterol β-Sitosterol (O940706H) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : methanol/water (100/2) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.64 AUFS Injection : 10 μL (0.4∼1 mg/mL) 78 (O940706C) Column : YMC-Pack ODS-AL (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : methanol/water (100/2) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.32 AUFS Injection : 10 μL (0.4∼1 mg/mL) ステロイド 8 Steroids 強心配糖体 Cardiac glycosides 強心配糖体 Cardiac glycosides 1 4 1 4 2 5 2 5 3 3 (S961125B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.13 AUFS Injection : 4 μL (0.05∼0.2 mg/mL) (S961126F) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.13 AUFS Injection : 4 μL (0.05∼0.2 mg/mL) 8 強心配糖体 Cardiac glycosides Steroids 強心配糖体 Cardiac glycosides 1 1 Lanatoside C 2 Ouabagenin 2 Proscillaridin A 3 G-Strophanthin(Ouabain) Lanatoside B (S961129G) Column : YMC-Pack Pro C18 (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.13 AUFS Injection : 8 μL (0.1∼0.2 mg/mL) (S961129I) Column : YMC-Pack Pro C18 (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 5 μL (0.2, 0.4 mg/mL) 79 ステロイド 8 Steroids センソ成分 Steroids from toad venom 1. O O O O H3C H3C OH HO H Bufalin 2. H3C 3 OCCH3 O H3C 2 O 1 HO H Cinobufagin O 3. O 4 H3C H3C O HO H Resibufogenin 4. H3CO 0 10 20 min Cl CO N CH3 CH2COOH Indomethacin (I.S.) (S010806A) Column : YMC-Pack ODS-AL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/0.1% phosphoric acid (45/55) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 300 nm, 0.08 AUFS Injection : 10 μL (0.04∼0.08 mg/mL) （日本薬局方第14改正記載条件） 8 ス テ ロ イ ド 80 医薬品、生体関連物質 9 Drugs, Metabolites 風邪薬成分 Ingredients in a cough/cold medication 市販風邪薬 A commercial cough/cold medication (F040616B) (F040616A) : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5) B) methanol 0-20%B(0-10min), 20-90%B(10-20min), 90%B(20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm(0-13min), UV at 235 nm(13-25min) Injection : 10 μL (12.5∼50.0 μg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5) B) methanol 0-20%B(0-10min), 20-90%B(10-20min), 90%B(20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm(0-13min), UV at 235 nm(13-25min) Injection : 15 μL (1.5 mg/mL) Sample : 70% methanol extract of a commercially available cough/cold medication 風邪薬成分 Ingredients in a cough/cold medication 市販風邪薬 A commercial cough/cold medication Column 9 Drugs, Metabolites (F040524B) (F040524A) Column Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5) B) methanol 0-20%B(0-10min), 20-90%B(10-20min), 90%B(20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm(0-12min), UV at 235 nm(12-25min) Injection : 10 μL (12.5∼50.0 μg/mL) : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5) B) methanol 0-20%B(0-10min), 20-90%B(10-20min), 90%B(20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm(0-12min), UV at 235 nm(12-25min) Injection : 15 μL (1.5 mg/mL) Sample : 70% methanol extract of a commercially available cough/cold medication 81 医薬品、生体関連物質 9 Drugs, Metabolites 市販風邪薬 A commercial cough/cold medication 市販風邪薬 A commercial cough/cold medication 1.Caffeine 2.Chlorpheniramine maleate 3.Narcotine 4.Ethenzamide 5.Bucetine (F040706B) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-K2HPO4 (pH 6.9) B) methanol 5-95%B(0-20min), 95%B(20-25min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 215 nm(0-13min), UV at 235 nm(13-25min) Injection : 2 μL (15 mg/mL) Sample : 70% methanol extract of a commercially available cough/cold medication 風邪薬成分 Ingredients in a cough/cold medication (G910523U) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 3 μL (10 mg/mL) Sample : an extract from a commercially available cough/cold preparation 風邪薬成分 Ingredients in a cough/cold medication 1.Acetaminophen 2.Caffeine 3.Chloropheniramine malate 4.Narcotine 5.Ethenzamide 6.Bucetine 9 医 薬 品 ・ 生 体 関 連 物 質 1.Acetaminophen 2.Caffeine 3.Chloropheniramine malate 4.Narcotine 5.Ethenzamide 6.Bucetine (G910523E) Column : YMC-Pack ODS-AM (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 10 μL (0.02∼0.22 mg/mL) 82 (G910523F) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/methanol/0.1% phosphoric acid (18/15/77) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 17 μL (0.02∼0.22 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 市販鎮痛解熱剤 Pain-killing drug インドメタシン Indometacin 1.Acetaminophen 2.Caffeine 3.Ethenzamide 4.Bromovalerylurea 1.p-Chlorobenzoic acid 2.p-Hydroxybenzoic acid n-butyl ester 3.Indometacin 2 1 3 (G900727F) (G900727D) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/0.1% phosphoric acid (70/30) Flow rate : 1.3 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.64 AUFS Injection : 20 μL Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/0.1% phosphoric acid (3/17) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (日本薬局方第12改正記載条件) 解熱鎮痛薬成分 Analgesics 解熱鎮痛薬成分 Analgesics 9 1 Drugs, Metabolites 1 Acetaminophen Acetaminophen 2 2 Caffeine 3 Caffeine 3 Ethenzamide Ethenzamide 4 4 Apronalide Apronalide (S930422D) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (40/60) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.51 AUFS Injection : 5 μL (0.2∼2.0 mg/mL) (S930422G) Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water (40/60) Flow rate : 0.7 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.51 AUFS Injection : 5 μL (0.2∼2.0 mg/mL) 83 医薬品、生体関連物質 9 Drugs, Metabolites 解熱鎮痛薬 Pain-killing drugs コデイン Codeine 2 3 4 1. H3C 1 YMC-Pack Pro C4 O 2 N N CH3 Caffeine 2. 3 CONH2 Codeine OC2H5 Ethenzamide 1 4 YMC-Pack Pro C8 CH3 N O N 3. H3C CHCHCONHCONH2 H3C Br Bromovalerylurea 2 3 4. CH3 CHCH2 CH3 1 YMC-Pack Pro C18 4 0 10 20 CH3 CHCOOH Ibuprofen 30 min (A001019B) Column : YMC-Pack Pro C4, Pro C8 and Pro C18 (5μm,12nm) 150 × 4.6 mm I.D. Eluent : methanol/20mM H3PO4 (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 10 μL (0.01∼2.0 mg/mL) Column 市販点鼻薬 A commercial nasal spray 1 A) Standard A) Standard CHCOOH CHCOOH 60 NH Tetrahydrozoline 2 CH3 CH3 10 0 10 CH2 Naphazoline 4 B) Nasal spray B) Nasal spray mAU 20 min F040128A Chlorpheniramine 20 min F040116F H 0 Cl 0 3 N 0 NHCCH2 N(CH2CH3 )2 CH3 1 N CHCH2CH2N CH3 O Lidocaine 20 3 2 2 40 40 1 CHCOOH CHCOOH Maleic acid 6 5 2 N 20 4 80 4 80 60 3 100 3 100 1 mAU Maleic acid mAU N 4 3 CH3 1 N 3 4 5 CHCH2CH2N mAU CH3 CH3 C CH2 C CH3 CH3 1 CH3 + (OCH2CH2)2 N CH2 Cl- CH3 CH3 CH3 Cl Chlorpheniramine 100 100 Benzethonium chloride 80 5 80 OH 医 薬 品 ・ 生 体 関 連 物 質 : YMC-Pack Pro C18 (5μm,12nm) 150 × 4.6 mm I.D. : acetonitrile/0.05% TFA (10/90) : 1.5 mL/min : UV at 210 nm : 5 μL (0.1 mg/mL) Eluent Flow rate Detection Injection 市販点鼻薬 A commercial nasal spray 9 (J010528A) 2 60 COOCH3 Methyl p-hydroxybenzoate 60 40 40 2 20 6 20 4 6 0 0 0 10 0 20 min 10 (F040119A) Column CH3 20 min CH3 + (OCH2CH2)2 N CH2 Cl- CH3 Benzethonium chloride F040119B F040119A : YMC-Pack Pro C18 (5μm,12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5) B) methanol 20-90%B(0-15min), 90%B(15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm Injection : A) 5 μL (0.1∼0.7 mg/mL) B) 3 μL (2 times dilution of a commercially available nasal spray with water) 84 CH3 CH3 C CH2 C CH3 CH3 (F040119B) Column : YMC-Pack Pro C18 (5μm,12nm) 150 × 4.6 mm I.D. Eluent : A) 20mM KH2PO4-H3PO4 (pH 2.5) B) methanol 20-90%B(0-15min), 90%B(15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm Injection : A) 5 μL (0.1∼0.7 mg/mL) B) 3 μL (2 times dilution of a commercially available nasal spray with water) 医薬品、生体関連物質 9 Drugs, Metabolites 鼻炎用点鼻剤 Collunarium 塩化ベンゼトニウム Benzethonium chloride 1.Benzethonium chloride 1 Naphazoline hydrochloride 2 Chlorpheniramine maleate 3 Benzethonium chloride Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. (Y920610A) Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. : YMC-Pack Ph (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/NH4SCN (48/52/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 2.56 AUFS Injection : 10 μL (0.2∼5 mg/mL) Sample : collunarium (T920527A) Column Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.16 AUFS Injection : 10 μL (1.0 mg/mL) 塩化ベンザルコニウム Benzalkonium chloride 塩化ベンザルコニウム Benzalkonium chloride 9 mAU Drugs, Metabolites CH3 1 80 + Cl- R N CH2 CH3 Benzalkonium chloride 1.Benzalkonium chloride R=-C12H25 2.Benzalkonium chloride R=-C14H29 2 60 1.R=-C12H25 2.R=-C14H29 3.R=-C16H33 3 40 20 0 0 5 10 min (F031210G) Courtesy of NIHON ARUTU PHARMACEUTICAL CO.,LTD. Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water/NH4SCN (70/30/0.5, v/v/w) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.02 AUFS Injection : 10 μL (1.0 mg/mL benzalkonium chloride) (T920529D) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 100mM NaCIO4/methanol (20/80) B) methanol 0-50%B(0-10min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm Injection : 10 μL (1.0 mg/mL) 85 医薬品、生体関連物質 9 Drugs, Metabolites 殺菌剤 Disinfectants クロルヘキシジン Chlorhexidine 1. CH3 CH3 mAU 1. CH3 + CH3 C CH2 C CH3 CH3 OCH2CH2OCH2CH2 N CH2 Cl- CH3 NH NH NH NH NHCNHCNH(CH2)6NHCNHCNH Cl Benzethonium chloride 80 Cl ・2HCl Chlorhexidine・2HCl 1 2. 1 CH3 + Cl- R N CH2 (R=C14H29) CH3 60 COOCH3 2. Benzalkonium chloride 2 Methyl benzoate 2 40 20 0 0 5 10 15 min 0 10 20 30 min (F031208D) (N040206D) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM NaCIO4/methanol (20/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm Injection : 10 μL (0.5∼1.0 mg/mL) : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (30/70/0.1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 10 μL (0.16 mg/mL, 0.1 μL/mL) 鼻炎薬成分 Ingredients in a drug for rhinitis 点眼剤成分 Ingredients in an eye drop Column 1. HO 9 CHCH2NHCH3 ·HCl 5 HO Phenylephrine HCl 1. 医 薬 品 ・ 生 体 関 連 物 質 HO 2. CHCOOH CHCOOH H 3C 5. CH2OH CH2OH N ・HCl N CHCH2CH2N Maleic acid Cl 5 HO 3. 2. 4 CH3 CH CHNH2 ·HCl 1 CH3 CH3 Pyridoxine HCl Chlorpheniramine CHCOOH CHCOOH Maleic acid 6. H3C Phenylpropanolamine HCl 3. 4. 4 O H3C N O 1 CH3 N O H3C CH3 CH3 + H3C N CH3 CH3SO4- O OCN(CH3)2 N N CH3 Neostigmine methylsulfate CH3 CH3 O H H3C CH 3 COOH O O H HH O H HO OH H H H H HO COOH H 4. 3 Caffeine H COOH OH OH H Glycyrrhizin H 5. N 2 Cl Chlorpheniramine 0 0 5 10 15 ·HCl Naphazoline HCl CH3 CHCH2CH2N CH3 2 CH2 N 6 N 3 5 10 15 min min (J010418B) Column : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5) B)acetonitrile 0-40%B(0-10min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.064 AUFS Injection : 3 μL (0.02∼0.5 mg/mL) 86 (J010406A) Column : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : A)20mM KH2PO4 - H3PO4 (pH 2.5) B)acetonitrile 0-60%B(0-10min), 60%B(10-15min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 265 nm, 0.128 AUFS Injection : 5 μL (0.03∼0.3 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 抗ヒスタミン薬 Histamine antagonists 抗ヒスタミン薬 Histamine antagonists 1. 1 COO(CH2)3CH3 1. N OH Butyl p -hydroxybenzoate (I.S.) CHCH2CH2N 1 2. 3 N Cl CHCOOH CH3 · CH3 CHCOOH Chlorpheniramine maleate CHCOOH CH3 CHCH2CH2N · CH3 CHCOOH 2. 2 Cl 2 Chlorpheniramine maleate CHOCH2CH2N Diphenhydramine hydrochloride 3. 3 CH3 · HCl CH3 CH3 CHOCH2CH2N · HCl CH3 3. HO Diphenhydramine hydrochloride Butyl p -hydroxybenzoate(I.S.) 0 0 5 10 15 COO(CH2)3CH3 5 10 min min (J010619A) (A980723A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (35/65) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.13 AUFS Injection : 5 μL (0.05∼2.0 mg/mL) Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : acetonitrile / 50mM CH3COONH4 (40/60) Flow rate : 0.2 mL/min Temperature : 37℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 2 μL (0.05∼2.0 mg/mL) 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 9 1 1 Drugs, Metabolites Piroxicam Fenoprofen calcium dihydrate 2 2 Sulindac Flurbiprofen 3 3 Tolmetin sodium dihydrate Ibuprofen 4 Ketoprofen 5 Naproxen (O950124A) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 2-propanol/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.04 AUFS Injection : 5 μL (20 μg/mL) (O950125C) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (65/35/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.04 AUFS Injection : 7 μL (160, 16, 820 μg/mL) 87 医薬品、生体関連物質 9 Drugs, Metabolites 酸性非ステロイド性抗炎症薬 Acidic non-steroidal anti-inflammatory drugs 酸性非ステロイド性解熱鎮痛抗炎症薬 - LC/MS Acidic non-steroidal anti-inflammatory drugs - LC/MS 1 Ketoprofen 2 Fenoprofen calcium dihydrate 3 Flurbiprofen 4 Ibuprofen (J040722B) (A970321A) : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (65/35/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.032 AUFS Injection : 7 μL (6∼820 μg/mL) Column : YMC-Pack Pro C18 (3μm, 12nm) 50 × 2.0 mm I.D. Eluent : methanol/water/CH3COOH (75/25/0.05) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : A) UV at 230 nm B) ESI negative-mode Injection : 1 μL (50 μg/mL) 三環系抗うつ薬 Tricyclic antidepressants 三環系抗うつ薬 Tricyclic antidepressants Column 9 1 1 医 薬 品 ・ 生 体 関 連 物 質 Doxepin hydrochloride Desipramine hydrochloride 2 2 Desipramine hydrochloride Acenaphthene (I.S.) 3 3 Imipramine hydrochloride Imipramine hydrochloride 4 4 Amitriptyline hydrochloride Amitriptyline hydrochloride (D960724A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 215 nm, 0.13 AUFS Injection : 2 μL (0.125 mg/mL) 88 (A961015G) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (0.1∼0.5 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 三環系抗うつ薬 Tricyclic antidepressants 三環系抗うつ薬 Tricyclic antidepressants 1. · HCl N 1 CH2CH2CH2NHCH3 Desipramine hydrochloride Desipramine hydrochloride 2. 2 2 · HCl 3 N CH3 CH3 Imipramine hydrochloride CH2CH2CH2N 1 4 Acenaphthene (I.S.) 3. · HCl 3 CH3 CH3 Amitriptyline hydrochloride CHCH2CH2N 4. Imipramine hydrochloride 4 Acenaphthene(I.S.) Amitriptyline hydrochloride 0 5 10 min (A980827E) (A961016B) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9)/methanol (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 5 μL (0.1∼0.5 mg/mL) 抗精神病薬 Antipsychotic drugs 1 2 Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol / 20mM CH3COONH4 (75/25) Flow rate : 1.0 mL/min Temperature : 37℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 5 μL (0.1∼0.5 mg/mL) 抗てんかん薬 Antiepileptics Perphenazine 9 1 3 Drugs, Metabolites Prochlorperazine maleate Ethosuximide 2 Promazine hydrochloride 4 Primidone Fluphenazine hydrochloride 5 3 Trifluoperazine hydrochloride 6 Phenobarbital sodium salt 4 Ethopropazine hydrochloride 7 (Profenamine hydrochloride) Phenytoin 8 5 Chlorpromazine hydrochloride Carbamazepine Thioridazine hydrochloride (O950627B) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (30/70/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.064 AUFS Injection : 5 μL (0.02 mg/mL) (S960723A) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/methanol (50/50) Flow rate : 0.7 mL/min Temperature : 37 ℃ Detection : UV at 215 nm, 0.32 AUFS Injection : 8 μL (0.035∼0.7 mg/mL) 89 医薬品、生体関連物質 9 Drugs, Metabolites 抗高脂血症剤 Hypolipidemic drugs 抗高脂血症剤 Hypolipidemic drugs 1 1 Bezafibrate Bezafibrate 2 2 Gemfibrozil Gemfibrozil 3 3 Clofibrate Clofibrate 4 4 Fenofibrate Fenofibrate (S930706E) (S930706C) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (60/40/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 275 nm, 0.064 AUFS Injection : 8 μL (0.05∼0.25 mg/mL) : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (60/40/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 275 nm, 0.064 AUFS Injection : 8 μL (0.05∼0.25 mg/mL) 抗高脂血症剤 Hypolipidemic drugs ロバスタチン Lovastatin 9 Column 1 医 薬 品 ・ 生 体 関 連 物 質 Bezafibrate 2 Gemfibrozil Lovastatin 3 Clofibrate 4 Fenofibrate (S930706B) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (60/40/1) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 275 nm, 0.064 AUFS Injection : 8 μL (0.05∼0.25 mg/mL) 90 (J010528B) Column Eluent Flow rate Detection Injection : YMC-Pack Pro C18 (5μm,12nm) 150 × 4.6 mm I.D. : acetonitrile/0.05% formic acid (30/70) : 1.5 mL/min : UV at 240 nm : 5 μL (0.1 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 酸性および塩基性医薬品(pH 6.8) Acidic and basic drugs (pH 6.8) 酸性および塩基性医薬品(pH 8.8) Acidic and basic drugs (pH 8.8) 1. CH3 CHCOOH 1. CH3 CHCOOH F mAU F Flurbiprofen 2. mAU 2. COOCH3 300 3. Methyl benzoate CH3 O 250 3. NHCCH2 N(CH2CH3 )2 CH3 1 4. 4 NHCCH2 N(CH2CH3 )2 CH3 1 2 4. OCOCH3 N O CH2CH2N(CH3)2 5 OCH3 3 S 150 Lidocaine 200 OCH3 3 CH3 O 250 Lidocaine 2 COOCH3 300 Methyl benzoate 200 Flurbiprofen S 4 ·HCl OCOCH3 N O CH2CH2N(CH3)2 150 Diltiazem HCl Diltiazem HCl 5. 100 H3CO OCH3 CH3 CN C (CH2)3NCH2CH2 H3CO 5. 100 H3CO OCH3 CH(CH3)2 50 ·HCl 5 CN ·HCl 50 Verapamil HCl 0 OCH3 CH3 C (CH2)3NCH2CH2 CH(CH3)2 H3CO OCH3 ·HCl Verapamil HCl 0 0 5 10 15 20 min 0 5 10 15 20 min (F040714E) (F040714C) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM CH3COONH4/methanol (35/65) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm Injection : 10 μL (0.04∼100 μg/mL) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM CH3COONH4-NH3(pH 8.8)/methanol (35/65) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm Injection : 10 μL (0.04∼100 μg/mL) 酸性および塩基性医薬品(pH 8.8) Acidic and basic drugs (pH 8.8) カルシウム拮抗薬 Calcium antagonists 5 1 1. CH3 CHCOOH Prenylamine lactate mAU Diltiazem HCl Flurbiprofen 12 300 3 2. 4 2 6 COOCH3 Methyl benzoate 3. CH3 O 250 Bepridil HCl Verapamil HCl 7 3 NHCCH2 N(CH2CH3 )2 CH3 Lidocaine 200 Methoxyverapamil HCl 4. OCH3 Niguldipine HCl 4 S 150 OCOCH3 N O CH2CH2N(CH3)2 5 Diltiazem HCl Nicardipine HCl Nifedipine 5. 100 H3CO H3CO CN CH3 C (CH2)3NCH2CH2 CH(CH3)2 50 8 ·HCl OCH3 OCH3 ·HCl Verapamil HCl 0 0 5 10 min (F040714D) Column : YMC-Pack Pro C18 RS (3μm, 8nm) 50 × 4.6 mm I.D. Eluent : 20mM CH3COONH4-NH3(pH 8.8)/methanol (35/65) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 220 nm Injection : 10 μL (0.04∼100 μg/mL) 9 Drugs, Metabolites F (S930702C) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA･Br (75/15/10/0.5, v/v/v/w) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.064 AUFS Injection : 8 μL (0.05∼1.4 mg/mL) 91 医薬品、生体関連物質 9 Drugs, Metabolites カルシウム拮抗薬 Calcium antagonists カルシウム拮抗薬 Calcium antagonists 5 1 1 5 Prenylamine lactate Prenylamine lactate Diltiazem HCl 6 2 Diltiazem HCl 2 6 Bepridil HCl Bepridil HCl Verapamil HCl Verapamil HCl 7 3 7 3 Methoxyverapamil HCl Niguldipine HCl 4 Methoxyverapamil HCl Niguldipine HCl 8 4 8 Nicardipine HCl Nicardipine HCl Nifedipine Nifedipine (S930701D) (S930702A) : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA･Br (70/20/10/0.35, v/v/v/w) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.064 AUFS Injection : 8 μL (0.05∼1.4 mg/mL) Column 不整脈治療薬 Antiarrhythmic drugs 不整脈治療薬 Antiarrhythmic drugs H N H O H O H O N 9 1. O N 1. : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - H3PO4 (pH 3.0)/acetonitrile/THF/TBA･Br (70/20/10/0.35, v/v/v/w) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.064 AUFS Injection : 8 μL (0.05∼1.4 mg/mL) N Column Phenytoin Phenytoin 医 薬 品 ・ 生 体 関 連 物 質 OCH2CHCH2NHCH(CH3)2 2. OCH2CHCH2NHCH(CH3)2 2. OH ·HCl OH ·HCl Propranolol HCl Propranolol HCl 2 H C CH3O CH CH2 3. CH CH2 3. H C 2 N OH CH3O N OH N Quinidine N Quinidine 4. 4. CH3 O CH3 O NHCCH2 N(CH2CH3 )2 CH3 NHCCH2 N(CH2CH3 )2 CH3 Lidocaine Lidocaine 1 4 5 3 OCOCH3 1 N O CH2CH2N(CH3)2 6 CN O CH2CH2N(CH3)2 4 Diltiazem HCl 6. H CO 3 OCH3 CH3 C (CH2)3NCH2CH2 H3CO 3 CH(CH3)2 H3C H3COOC H N OCH3 OCH3 CH3 OCH3 CH(CH3)2 ·HCl Verapamil HCl ·HCl 6 CH3 COOCH2CH2N CN C (CH2)3NCH2CH2 H3CO Verapamil HCl 7. OCOCH3 N 5 Diltiazem HCl 6. H CO 3 ·HCl S ·HCl S 7 OCH3 5. OCH3 5. 7 7. H3C H3COOC CH2 CH3 H N CH3 COOCH2CH2N CH2 CH3 ·HCl ·HCl NO2 NO2 Nicardipine HCl Nicardipine HCl 0 10 20 30 min 0 (S990819B) Column : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 5 μL (0.05∼0.1 mg/mL) 92 10 20 min (S990817C) Column : YMC-Pack Pro C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (40/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 5 μL (0.05∼0.1 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 局所麻酔薬 Local anesthetics 1. 利尿薬 Diuretics O H 2N COCH2CH3 Benzocaine (Ethyl 4-aminobenzoate) 2. 1 O H 2N COCH2CH2N(CH2CH3)2 ·HCl Procaine HCl 3. CH2 CH HO C H H H Hydrochlorothiazide N 2 N 1 Cinchonine 2 4. CH3 O NHCCH2 N(CH2CH3 )2 CH3 Chlorthalidone Lidocaine O 5 5. 4 3 COCH2CH2N(CH3)2 3 ·HCl 6 NHCH2CH2CH2CH3 Tetracaine HCl 6. Furosemide O H 2N COCH2CH2CH2N(C4H9)2 Butacaine 0 5 10 15 min (J010302E) (A970425C) Column : Hydrosphere C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / methanol (40/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.02∼0.5 mg/mL) Column 利尿薬 Diuretics 利尿薬 Diuretics : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 - H3PO4(pH 3.2)/methanol (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.16 AUFS Injection : 15 μL (0.015∼0.2 mg/mL) 1. 1 9 O N H 3 Hydrochlorothiazide 2 Hydrochlorothiazide O S NH Drugs, Metabolites O H2N S O Cl O 2. 2 NH HO 1 Cl SO2NH2 Chlorthalidone Chlorthalidone 3. 3 O H2N S O CO2H NHCH2 O Cl Furosemide Furosemide 0 5 10 min (A970426D) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 - H3PO4(pH 3.2)/methanol (60/40) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.16 AUFS Injection : 15 μL (0.015∼0.2 mg/mL) (A980716A) Column : YMC-Pack Pro C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : methanol / 1% acetic acid (40/60) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.08 AUFS Injection : 3 μL (0.015∼0.2 mg/mL) 93 医薬品、生体関連物質 9 Drugs, Metabolites 制がん剤および関連化合物 Antineoplastic and related compounds 制がん剤および関連化合物 Antineoplastic and related compounds 1 1 Uracil Uracil 2 2 5-Fluorouracil 5-Fluorouracil 3 3 Deoxyuridine Deoxyuridine 4 4 5-Fluoro-2'-deoxyuridine 5-Fluoro-2'-deoxyuridine (T920924E) (T920924G) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 10mM NH4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.025∼0.2 mg/mL) Column 葉酸及び葉酸拮抗物質 Folic acid and its antagonists 抗ＨＩＶヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents 9 : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 10mM NH4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.025∼0.2 mg/mL) 1 医 薬 品 ・ 生 体 関 連 物 質 1 Aminopterin 2 DDC 2 Methotrexate 3 DDI Folic acid 3 D4T 4 AZT (O950411B) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Temperature : ambient(25 ℃) Detection : UV at 300 nm, 0.04 AUFS Injection : 5 μL (16 μg/mL) 94 (K930311C) Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 7 μL (0.125 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 抗ＨＩＶヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents 抗ＨＩＶヌクレオシド誘導体 Anti-human immunodeficiency virus (HIV) agents 1 1 DDC DDC 2 2 DDI DDI 3 3 D4T D4T 4 4 AZT AZT (K930311A) (K930311F) Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 7 μL (0.125 mg/mL) Column : YMC-Pack C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM KH2PO4 (10/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 7 μL (0.125 mg/mL) ニコチン酸構造類縁体 Nicotinic acid analogues ニコチン酸構造類縁体 Nicotinic acid analogues 1 9 1 Isonicotinic acid 2 Nicotinic acid Nicotinic acid 3 3 Isonicotinic acid hydrazide Isonicotinic acid hydrazide 4 4 Nicotinamide Nicotinamide 5 5 N' -Methylnicotinamide N' -Methylnicotinamide (K940125I) Column Drugs, Metabolites Isonicotinic acid 2 : YMC-Pack ODS-A (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.64 AUFS Injection : 13 μL (0.2 mg/mL) (K940125K) Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.64 AUFS Injection : 13 μL (0.2 mg/mL) 95 医薬品、生体関連物質 9 Drugs, Metabolites 塩基性化合物 Basic compounds 殺菌薬 Disinfectants 1 1 Benzoic acid 2 Uracil 2 Salicylic acid Procainamide 3 3 N-Acetylprocainamide Theophylline (I.S.) 4 4 Phenol 4'-Hydroxyacetophenone (S930617D) (T931217C) Column Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM K2HPO4 - KH2PO4 (pH 6.9)/acetonitrile (92/8) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 10 μL (30∼300 μg/mL) アシクロビル Acyclovir : YMC-Pack ODS-AL (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 100mM KH2PO4 - Na2HPO4 (pH 7.0)/methanol (75/25) Flow rate : 0.8 mL/min Temperature : 30 ℃ Detection : UV at 270 nm, 0.13 AUFS Injection : 3 μL (0.1∼0.8 mg/mL) 日本薬局方第12改正記載条件 塩化カルニチン Carnitine chloride 9 2 1 2. (CH3)3N+CH2CHCH2COOH Cl OH Carnitine chloride H N 1. 医 薬 品 ・ 生 体 関 連 物 質 N Acyclovir CH2CHCOOH ·HCl NH2 L-Histidine 0 Column Eluent Flow rate Detection Injection 96 : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. : acetonitrile / 0.05% formic acid (3 / 97) : 1.5 mL/min : UV at 254 nm : 5μL (0.1 mg/mL) 10 15 20 min (F000629A) (J010528C) Column 5 hydrochloride : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NaH2PO4-H3PO4 (pH2.6) containing 15mM 1-pentanesulfonic acid sodium salt Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 210 nm, 0.064 AUFS Injection : 4 μL (0.2∼20 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites キサンチンオキシダーゼ阻害剤および関連化合物 Xanthine oxidase inhibitor and related compounds キサンチンオキシダーゼ阻害剤および関連化合物 Xanthine oxidase inhibitor and related compounds 1 1 Uric acid Uric acid 2 2 Hypoxanthine Hypoxanthine 3 3 Xanthine Xanthine 4 4 Oxipurinol Oxipurinol 5 5. Allopurinol Allopurinol (S930728J) (S930729B) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.51 AUFS Injection : 8 μL (0.05∼0.2 mg/mL) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.51 AUFS Injection : 8 μL (0.05∼0.2 mg/mL) カフェイン代謝物 Caffeine metabolites 尿中のカフェイン代謝物 Caffeine metabolites in human urine 9 Drugs, Metabolites (F041213B) (F041213A) Column Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/0.75% formic acid (4/1) Flow rate : 1.0 mL/min Temperature : ambient Detection : A) UV at 255 nm, B) UV at 295nm Injection : 10 μL : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : acetonitrile/0.75% formic acid (4/1) Flow rate : 1.0 mL/min Temperature : ambient Detection : A) UV at 255 nm, B) UV at 295nm Injection : Standard 10 μL Urine sample 10 μL 97 医薬品、生体関連物質 9 Drugs, Metabolites プリン代謝物 Purine catabolites プリン代謝物 Purine catabolites 1.Allantoin 2.Allantoic acid 3.Uric acid 1.Allantoin 2.Allantoic acid 3.Uric acid (G910607G) (G910618A) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : 10mM CH3COOH Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 200 nm, 0.16 AUFS Injection : 4 μL (0.10∼0.71 mg/mL) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : 10mM CH3COOH Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 200 nm, 0.16 AUFS Injection : 4 μL (0.10∼0.71 mg/mL) カテコールアミン Catecholamines 馬尿酸類 Hippuric and methylhippuric acids 9 1 医 薬 品 ・ 生 体 関 連 物 質 1. Norepinephrine hydrochloride 2. Epinephrine 3. 3,4-Dihydroxyphenylalanine (DOPA) 4. Dopamine hydrochloride 5. 4-Hydroxy-3-methoxymandelic acid 6. 3-Methoxytyramine hydrochloride 7. 4-Hydroxy-3-methoxyphenylglycol 8. 3,4-Dihydroxyphenylacetic acid 9. Homovanillic acid 1 3 2 4 2 Hippuric acid 3 8 6 5 Mandelic acid o-Methylhippuric acid 7 9 4 m-Methylhippuric acid 5 0 10 20 30 40 min p-Methylhippuric acid (F000817A) : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)50mM KH2PO4-H3PO4(pH 3.1) B)50mM KH2PO4-H3PO4(pH 3.1)/acetonitrile (90/10) 0%B (0-5min), 0-100%B (5-25min,linear), 100%B (25-35min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 280 nm, 0.64 AUFS Injection : 10 μL (0.15∼0.25 mg/mL) (A961114A) Column 98 Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 225 nm, 0.16 AUFS Injection : 10 μL (0.04∼0.07 mg/mL) 医薬品、生体関連物質 9 Drugs, Metabolites 馬尿酸類 Hippuric and methylhippuric acids 1 Mandelic acid 2 Hippuric acid 3 o-Methylhippuric acid 4 m-Methylhippuric acid 5 p-Methylhippuric acid (A961119A) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 - H3PO4 (pH 3.2)/methanol (80/20) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 225 nm, 0.16 AUFS Injection : 10 μL (0.04∼0.07 mg/mL) 9 Drugs, Metabolites 99 抗菌剤、抗生物質 10 Antibiotics 合成抗菌剤 Antibacterial agents 合成抗菌剤 Antibacterial agents 1. Olaquindox 2. Carbadox 3. Thiamphenicol 4. Sulfamerazine 5. Sulfadimidine 6. Sulfamonomethoxine 7. Furazolidone 8. Oxolinic acid 9. Sulfaquinoxaline 10. Sulfadimethoxine 11. Nalidixic acid 12. Piromidic acid 1. Olaquindox 2. Carbadox 3. Thiamphenicol 4. Sulfamerazine 5. Sulfadimidine 6. Sulfamonomethoxine 7. Furazolidone 8. Oxolinic acid 9. Sulfaquinoxaline 10. Sulfadimethoxine 11. Nalidixic acid 12. Piromidic acid (K940614B) (K940615C) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) B)acetonitrile/50mM NH4H2PO4 (80/20) 25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.32 AUFS Injection : 10 μL (0.05 mg/mL) Column : J'sphere ODS-L80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) B)acetonitrile/50mM NH4H2PO4 (80/20) 25%B (0-5min), 25-100%B (5-15min), 100%B (15-20min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.32 AUFS Injection : 10 μL (0.05 mg/mL) 合成抗菌剤 Antibacterial agents サルファ剤 Sulfonamides 1 1 6 Sulfaguanidine 2 7 Sulfapyridine Diaveridine Sulfanilamide 2 3 10 Sulfisomidine Trimethoprim 抗 菌 剤 ・ 抗 生 物 質 8 4 9 Sulfamerazine Sulfamethizole 3 Sulfathiazole 5 Pyrimethamine 10 Sulfadiazine (O950320G) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 5 μL (20 μg/mL) 100 Sulfamethoxypyridazine Sulfamethazine (O950306E) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (10/90) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 3 μL (50 μg/mL) 抗菌剤、抗生物質 10 Antibiotics サルファ剤 Sulfonamides サルファ剤 Sulfonamides 1 1 6 Sulfamethazine 2 Sulfaguanidine 2 Sulfathiazole 7 Sulfadoxine 3 Sulfamethizole 3 Trimethoprim 8 Sulfamonomethoxine hydrate Sulfanilamide 4 4 Sulfapyridine 9 Sulfisomidine 5 Sulfamerazine 5 10 Sulfadiazine Sulfamethoxypyridazine 6 11 Sulfisoxazole Sulfamethoxazole Sulfachloropyridazine Sulfamethazine 7 8 Sulfadimethoxine Sulfaquinoxaline sodium (O950324L) (O950302C) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.2)/THF (90/10) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 10 μL (18 μg/mL) Column : YMC-Pack ODS-A (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : THF/water/acetic acid (30/70/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 5 μL (42 μg/mL) サルファ剤 Sulfonamides サルファ剤 Sulfonamides 1. NH2 N SO2NH CH3 N 5. N NH2 SO2NH N CH3 1 6 Sulfisomidine 2 Sulfisomidine Sulfamonomethoxine hydrate 7 2. NH2 1 OCH3 Sulfamonomethoxine 6. S SO2NH NH2 N Sulfathiazole Sulfathiazole 3 3. 7. N Sulfamerazine Sulfamethoxazole 9 2 3 Sulfadimethoxine N 4. N NH2 Sulfaquinoxaline sodium N NH2 SO2NH N SO2NH CH3 10 OCH3 N OCH3 Sulfadimethoxine CH3 CH3 Sulfadimidine 4 Sulfamethazine SO2NH Sulfamerazine 10 5 CH3 Antibiotics 4 O Sulfamethoxazole Sulfadoxine 8 NH2 Sulfadiazine SO2NH N 5 7 6 (O950217D) 0 5 10 15 20 min (J010321F) Column Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/acetonitrile/50mM NH4H2PO4 (15/5/80) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 3 μL (50 μg/mL) : Hydrosphere C18 (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : 10mM CH3COOH - CH3COONH4 (pH 4.6) / acetonitrile (85/15) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 10 μL (0.01∼0.04 mg/mL) 101 抗菌剤、抗生物質 10 Antibiotics サルファ剤 Sulfonamides 抗生物質 Antibiotics N N SO2NH NH2 N N Sulfamonomethoxine 2. NH2 SO2NH OCH3 Sulfadiazine 1 1.Ampicilline 2.Cephalexin 3.Oxytetracycline hydrochloride 4.Tetracycline 5.Chlorotetracycline 6.Chloramphenichol 7.Oxolinic acid 8.Doxorubicin hydrochloride 9.Nalidixic acid 5. 1. NH2 6. S SO2NH NH2 SO2NH N N Sulfathiazole O CH3 Sulfamethoxazole 2 3. N 3 NH2 SO2NH N CH3 Sulfamerazine 4 4. N NH2 5 SO2NH N CH3 CH3 Sulfadimidine 6 0 5 10 min (G910523M) (J010130D) : Hydrosphere C18 (3μm, 12nm) 75 × 2.0 mm I.D. Eluent : 10mM CH3COOH - CH3COONH4 (pH 4.6) / acetonitrile (85/15) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 275 nm, 0.16 AUFS Injection : 2 μL (0.02 mg/mL) : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (10/90) B)acetonitrile/50mM NH4H2PO4 (50/50) 0-55%B (0-30min,linear), 55%B (30-40min) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 250 nm, 0.16 AUFS Injection : 15 μL (0.01∼0.15 mg/mL) ナリジクス酸系抗生物質 Nalidixic acid antibiotics ナリジクス酸系抗生物質 Nalidixic acid antibiotics Column 1 Column 1 Pipemidic acid Pipemidic acid 2 2 Enoxacin Enoxacin 3 10 3 Cinoxacin Cinoxacin 4 抗 菌 剤 ・ 抗 生 物 質 4 Norfloxacin Norfloxacin 5 5 Ofloxacin Ofloxacin 6 6 Nalidixic acid Nalidixic acid 7 7 Oxolinic acid Oxolinic acid 8 8 Piromidic acid Piromidic acid (O950428A) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10) containing 20mM TBA･Br Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 5 μL (0.03 mg/mL) 102 (O950428D) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/tetrahydrofuran (90/10) containing 20mM TBA･Br Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 5 μL (0.03 mg/mL) 抗菌剤、抗生物質 10 Antibiotics ナリジクス酸系抗生物質 Nalidixic acid antibiotics ナリジクス酸系抗生物質 Nalidixic acid antibiotics O 1. COOH O O 1. COOH O N N O C2H5 N N C2H5 O Cinoxacin O 2. Cinoxacin COOH O O 2. O COOH O O N C2H5 N C2H5 Oxolinic acid 1 3. Oxolinic acid O 1 3. O 2 H3C 3 H3C 2 COOH C2H5 3 N N Nalidixic acid C2H5 4. Nalidixic acid 4 4. O N N N COOH N N O N 4 N COOH N COOH N C2H5 N Piromidic acid C2H5 Piromidic acid 0 5 10 15 0 min 5 10 15 min (A010324A) (A010324C) Column : Hydrosphere C18 (3μm, 12nm) 50 × 2.0 mm I.D. Eluent : acetonitrile / 50mM NaH2PO4 (25/75) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.128 AUFS Injection : 1 μL (0.02∼0.1 mg/mL) : Hydrosphere C18 (3μm, 12nm) 50 × 2.0 mm I.D. Eluent : 10mM CH3COOH - CH3COONH4(pH 4.3) / acetonitrile (75/25) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.128 AUFS Injection : 1 μL (0.02∼0.1 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics Column 1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 10. Cefuroxime sodium 11. Cefoxitin sodium 1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 10. Cefuroxime sodium 11. Cefoxitin sodium 10 Antibiotics (O940719A) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.12∼0.35 mg/mL) (O940715F) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.12∼0.35 mg/mL) 103 抗菌剤、抗生物質 10 Antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1. Cefsulodin sodium 2. Cephalexin hydrate 3. Cefaclor 4. Cephradine 5. Cephaloglycin 6. Cephapirin sodium 7. Cefotaxime sodium 8. Cephaloridine 9. Cefazolin sodium 10. Cefuroxime sodium 11. Cefoxitin sodium 1 Cefadroxil 2 Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 Cephaloridine (O940718B) (O940927B) Column : J'sphere ODS-L80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.12∼0.35 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Cefadroxil Cefadroxil 2 Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 2 10 Cephalosporin C potassium 抗 菌 剤 ・ 抗 生 物 質 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 Cephaloridine Cephaloridine (O940927C) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) 104 (O940927I) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) 抗菌剤、抗生物質 10 Antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Cefadroxil Cefadroxil 2 2 Cephalosporin C potassium Cephalosporin C potassium 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 3 Cefatrizine propylene glycol 4 Cephalexin hydrate 5 Cephaloridine Cephaloridine (O940927G) (O941025E) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/water/acetic acid (10/85/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.2 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Cefoperazone sodium Cefoperazone sodium 10 2 2 Antibiotics Cephalothin sodium Cephalothin sodium 3 3 Cefamandole sodium Cefamandole sodium (O940928P) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) (O940928Q) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) 105 抗菌剤、抗生物質 10 Antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Cefoperazone sodium Cefoperazone sodium 2 2 Cephalothin sodium Cephalothin sodium 3 3 Cefamandole sodium Cefamandole sodium (O940928R) (O940929D) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 2.0 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 3 μL (0.17 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Ceftriaxone sodium Cefoperazone sodium 10 2 2 抗 菌 剤 ・ 抗 生 物 質 Cefotaxime sodium Cephalothin sodium 3 Cefamandole sodium (O940928S) Column : YMC-Pack ODS-A (3μm, 12nm) 50 × 4.6 mm I.D. Eluent : THF/50mM NH4H2PO4 (15/85) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 6 μL (0.17 mg/mL) 106 (O940928G) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.08, 0.16 mg/mL) 抗菌剤、抗生物質 10 Antibiotics セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 Ceftriaxone sodium Ceftriaxone sodium 2 2 Cefotaxime sodium Cefotaxime sodium (O940928F) (O940929K) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : methanol/50mM NH4H2PO4 (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 5 μL (0.08, 0.16 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 2.0 mm I.D. Eluent : methanol/50mM NH4H2PO4 (30/70) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 3 μL (0.08, 0.16 mg/mL) セフェム系抗生物質 Cephalosporin antibiotics セフェム系抗生物質 Cephalosporin antibiotics 1 1 S CH CO NH NH2 O N Cephalexin 1 2 Cephaloglycin S CH CO NH 2 NH2 3 O N 3 S CH CO NH NH2 Cefotaxime sodium 4 O N CH2OCOCH3 COOH Cephaloglycin 3 4 S N CH2 +N COOH Cephaloridine Cephapirin sodium 0 5 10 15 20 min (O940720A) : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.64 AUFS Injection : 5 μL (0.5 mg/mL) S CH2 CO NH O Column 10 Cl COOH Antibiotics Cefaclor 2 CH3 COOH (S000717B) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 15 μL (0.1∼0.2 mg/mL) 107 抗菌剤、抗生物質 10 Antibiotics セファロスポリンC Cephalosporin C セフトリアキソン Ceftriaxone 1. 1. H2N CH (CH2)3OCNH HOOC O 1 S N N CH2 O COCH3 COOK H2N 1 S C CO NH NOCH3 O Cephalosporin C potassium S N H3C N N CH2S COONa N OH O Ceftriaxone sodium 2. OH 2 OH 2. OH Resorcinol Phenol (I.S.) 2 0 5 10 15 0 min 5 10 15 min (S000714B) (S000726B) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM KH2PO4 (5/95) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 10μL (0.2 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol / 10mM H3PO4 (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.1, 0.4 mg/mL) セフォキシチンナトリウム Cefoxitin sodium セフォキシチンナトリウム Cefoxitin sodium 1 1 Caffeine (I.S.) 10 Caffeine (I.S.) 2 抗 菌 剤 ・ 抗 生 物 質 2 Cefoxitin sodium Cefoxitin sodium (O940728M) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/50mM KH2PO4 (20/80) Flow rate : 1.8 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 μL (8, 12 mg/mL) 108 (O940728P) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 2.0 mm I.D. Eluent : acetonitrile/50mM KH2PO4 (20/80) Flow rate : 0.34 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 μL (1.6, 2.4 mg/mL) 抗菌剤、抗生物質 10 Antibiotics セフォキシチンナトリウム Cefoxitin sodium ペニシリン系抗生物質 Penicillin antibiotics 1 2 Bacampicillin hydrocloride 1 Methicillin sodium 3 Caffeine (I.S.) 4 Penicillin G potassium (Benzylpenicillin potassium) 2 5 Penicillin V potassium (Phenoxymethylpenicillin potassium) Cefoxitin sodium Phenethicillin potassium 6 (Phenoxyethylpenicillin potassium) 7 Cloxacillin sodium Propicillin potassium 8 Dicloxacillin sodium (O940729A) (O950908A) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 2.0 mm I.D. Eluent : acetonitrile/50mM KH2PO4 (20/80) Flow rate : 0.34 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.32 AUFS Injection : 1.2 μL (1.6, 2.4 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (67/33) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.26 AUFS Injection : 8 μL (0.13 mg/mL) ペニシリン系抗生物質 Penicillin antibiotics アモキシシリン Amoxicillin 1 1. S CH CO NH HO NH2 Amoxicillin hydrate O N CH3 CH3 COOH Amoxicillin 2 1 2. H3C 2 O Ampicillin anhydrous O N CH3 N 10 N Antibiotics N CH3 Caffeine 0 5 10 15 min (O950920C) (S000622D) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 50mM NaH2PO4 - H3PO4 (pH 2.3)/acetonitrile (80/20) containing 20mM sodium 1-heptanesulfonate Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.13 AUFS Injection : 2 μL (0.3, 0.7 mg/mL) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile / water / TFA (10/90/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 10 μL (0.2, 0.1 mg/mL) 109 抗菌剤、抗生物質 10 Antibiotics 抗生物質の高速分離 - LC/MS Rapid separation of antibiotics - LC/MS 2 100 エリスロマイシン Erythromycin antibiotic drugs 1.Erythromycin 2.Erythromycin ethylsuccinate 3.Erythromycin estolate 1. S CH CO NH HO NH2 N O CH3 CH3 COOH 1 Amoxicillin (MW:365.1) 2. S CH CO NH % (m/z) TIC NH2 O N CH3 COOH Cephalexin (MW:347.1) 366 348 0 0 2.0 min (A020620A) Column : LC/MS Cartridge Column VA Pro C18 (3μm, 12nm) 20 × 2.0 mm I.D. Eluent : A) water/formic acid (100/0.05) B) methanol/formic acid (100/0.05) 10-90%B(0-0.5min), 90%B(0.5-1.0min) Flow rate : 0.6 mL/min Temperature : 37 ℃ Detection : ESI-positive mode Injection : 2 μL : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/methanol/100mM (NH4)2HPO4(45/15/40) Flow rate : 1.0 mL/min Temperature : 45 ℃ Detection : UV at 210 nm, 0.26 AUFS Injection : 15 μL (2.5 mg/mL) テトラサイクリン系抗生物質 Tetracycline antibiotics バンコマイシン Vancomycin (S930910B) Column OH 1. H N O 1 OH OH CO2H NH HO O HN Cl 2 10 CH2OH Oxytetracycline hydrochloride 1 Tetracycline hydrochloride O O CNH2 N H O HN O O HH OH H Cl HO H H H O O H CH 3 ・HCl HO CH3 H NH2 2 抗 菌 剤 ・ 抗 生 物 質 O O O NH OH CH3 NH O H3C CH3 Vancomycin hydrochloride 2. O C CH3 OH 4'-Hydroxyacetophenone 0 10 20 min (S000728B) (S970524D) Column Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/10mM oxalic acid (10/90) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 280 nm, 0.13 AUFS Injection : 6 μL (0.2 mg/mL) 110 : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH 6.9) / acetonitrile (88/12) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 220 nm, 0.32 AUFS Injection : 10 μL (0.1 mg/mL) 農薬、環境関連物質 11 Pesticides, Environmental ベンスリド O,O -Di-isopropyl S-2-benzenesulphonamidoethylphosphorodithioate チウラム Bis(dimethylthiocarbamoyl)disulfide 1.Thiram 1.Bensulide (G910223E) (G910223D) Column : YMC-Pack MB-ODS 150 × 2.1 mm I.D. Eluent : acetonitrile/water (60/40) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 220 nm, 0.01 AUFS Injection : 10μL (1.0 mg/L) Column : YMC-Pack MB-ODS 150 × 2.1 mm I.D. Eluent : acetonitrile/water (40/60) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.01 AUFS Injection : 10 μL (2.0 mg/L) アシュラム Methyl sulfanilylcarbamate オキシン銅 Copper 8-quinolinolate 1.Asulam 1. Copper 8-quinolinolate 11 5 10 min (G910223C) Column : YMC-Pack MB-ODS 150 × 2.1 mm I.D. Eluent : THF/water/TFA (10/90/0.05) Flow rate : 0.3 mL/min Temperature : 30 ℃ Detection : UV at 260 nm, 0.01 AUFS Injection : 10 μL (1.0 mg/L) (A010717A) Column : Hydrosphere C18 (5μm, 12nm) 150 × 2.0 mm I.D. Eluent : acetonitrile/20mM H3PO4 (5/95) Flow rate : 0.2 mL/min Temperature : 37 ℃ Detection : UV at 250 nm, 0.32 AUFS Injection : 2 μL (0.02 mg/mL) 111 Pesticides, Environmental 0 農薬、環境関連物質 11 Pesticides, Environmental 水質規制農薬9成分 Analysis of 9 pesticides 除草剤 Herbicides 5 1 S S (CH3)2NCSSCN(CH3)2 O N Cu N O 4 3 Oxine-copper (Copper 8-quinolinolate) 5 1. Atrazine-desisopropyl 2. Atrazine-desethyl 3. Crimidine 4. Hexazinone 5. Metoxuron 6. Simazine 7. Cyanazine 8. Atraton 9. Atrazine 10. Isoproturon 11. Diuron 12. Prometon 13. Metobromuron 14. Terbumeton 15. Metazachlor 16. Ametryn 17. Sebuthylazine 18. Propazine 19. Terbuthylazine 20. Linuron 21. Prometryn 22. Terbutryn 23. Metolachlor Thiram 6 2 H N CH3 O NHCOCH3 O OCHCOH CH3 Cl N Mecoprop (MCPP) Carbendazim (MBC) 2 7 3 67 8 H3C O NHCNH O O SNHCOCH3 O H2 N Asulam Siduron 8 4 S O NHCNHCOCH3 Cl NHCNHCOCH3 S O Cl O O CHNHCH(CH3)2 N N 9 O Iprodion Thiophanate-methyl 9 1 O S OCH(CH3)2 SNHCH2CH2SP OCH(CH3)2 O Bensulide (SAP) 0 10 20 min (N040107A) (O941018B) Column : Hydrosphere C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A) 50mM KH2PO4-H3PO4 (pH 3.0) B) acetonitrile 15-65%B (0-10min), 65%B (10-20min) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 270 nm (0-6.5 min), 230 nm (6.5-20 min) 0.08 AUFS Injection : 10 μL (10 μg/mL) : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : A)acetonitrile/50mM NH4H2PO4 (20/80) B)acetonitrile/50mM NH4H2PO4 (65/35) 0-100%B (0-60min, linear) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 5 μL (0.04 mg/mL) アトラジン誘導体 Atrazine derivatives トリアジン系除草剤 Triazine herbicides Column 1. Cyanazine 2. Simazine 3. Atraton 4. Atrazine 5. Prometon 6. Terbumeton 7. Sebuthylazine 8. Propazine 9. Terbuthylazine 10. Prometryn 11. Terbutryn 1 Atrazine-2-hydroxy 2 Atrazine-desisopropyl 3 11 Atrazine-desethyl 農 薬 ・ 環 境 関 連 物 質 4 Atrazine (O941020H) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/TFA (25/75/0.1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.16 AUFS Injection : 5 μL (0.04 mg/mL) 112 (O941102F) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 5 μL (0.05 mg/mL) 農薬、環境関連物質 11 Pesticides, Environmental トリアジン系除草剤 Triazine herbicides 尿素系除草剤 Urea herbicides 1 5 Sebuthylazine Simazine 2 1 Metoxuron 6 2 Propazine Atrazine 3 Isoproturon 7 Terbuthylazine Prometon 4 3 Diuron 8 4 Prometryn Terbumeton Metobromuron 9 5 Terbutryn Linuron (A970210B) (O941028B) Column : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/50mM NH4H2PO4 (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 5 μL (0.05 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : THF/water (30/70) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.16 AUFS Injection : 4 μL (0.2 mg/mL) 酸アミド系除草剤 Acid amide herbicides カルバメート系除草剤 Carbamate herbicides 1 1 Propham Metazachlor 2 2 Swep Metolachlor 3 3 11 Alachlor 4 Butachlor (O941108C) Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 2-propanol/THF/water (25/15/60) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 210 nm, 0.16 AUFS Injection : 8 μL (0.05 mg/mL) (O941124G) Column : J'sphere ODS-L80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water (45/55) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.16 AUFS Injection : 5 μL (0.08 mg/mL) 113 Pesticides, Environmental Chloropropham 農薬、環境関連物質 11 Pesticides, Environmental カルバメート系殺虫剤 Carbamate insecticides 防かび剤 Fungicides O 1. O (CH3)2NC C NOCNHCH3 SCH3 1 Oxamyl 2. O Thiabendazole CH3S C(CH3)2CH NOCNHCH3 2 Aldicarb 2 1 O 3. OCNHCH3 O CH 3 3 o-Phenylphenol CH3 O Bendiocarb 5 3 O 4. OCNHCH3 CH2SC2H5 4 Ethiofencarb O 5. Imazalil N(CH3)2 N (CH3)2NCO N H3C 4 CH3 Pirimicarb Diphenyl 0 5 10 min (J010220D) (A970217A) Column : Hydrosphere C18 (3μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol / 20mM KH2PO4 (45/55) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 240 nm, 0.64 AUFS Injection : 5 μL (0.1∼1.5 mg/mL) : YMC-Pack Pro C18 (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 10 μL (0.02∼0.20 mg/mL) 防かび剤 Fungicides イマザリル Imazalil Column 1.Imazalil 1 Thiabendazole 2 o-Phenylphenol 3 11 農 薬 ・ 環 境 関 連 物 質 Imazalil 4 Diphenyl (A970219A) Column : YMC-Pack ODS-A (5μm, 12nm) 75 × 4.6 mm I.D. Eluent : methanol/20mM NH4H2PO4 (70/30) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 10 μL (0.02∼0.20 mg/mL) 114 (B931007A) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/20mM NH4H2PO4 (50/50) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.04 AUFS Injection : 10 μL (0.05 mg/mL) 農薬、環境関連物質 11 Pesticides, Environmental 陰イオン界面活性剤 Anionic surfactants 市販洗濯洗剤中の陰イオン界面活性剤 Anionic surfactants in a commercial laundry detergent A) Standard mV C nH 2n+1 mV C nH 2n+1 1 SO3Na 300 200 3 4 3 200 4 5 100 0 20 10 0 N040326B min 1. Sodium decylbenzenesulfonate (n=10) 2. Sodium undecylbenzenesulfonate (n=11) 3. Sodium dodecylbenzenesulfonate (n=12) 4. Sodium tridecylbenzenesulfonate (n=13) 5. Sodium tetradecylbenzenesulfonate (n=14) B) Laundry detergent 5 100 SO3Na 2 1. Sodium decylbenzenesulfonate (n=10) 2. Sodium undecylbenzenesulfonate (n=11) 3. Sodium dodecylbenzenesulfonate (n=12) 4. Sodium tridecylbenzenesulfonate (n=13) 5. Sodium tetradecylbenzenesulfonate (n=14) 2 1 mV 2 3 300 200 1 0 4 100 0 20 10 min 0 0 N040325A min 20 10 (N040325A) (N040324D) 平成15年改正水質基準の検査方法準拠 : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) containing 0.1M NaClO4 Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : FLS at Ex 221 nm, Em 284 nm Injection : 20 μL Sample : A) 2.0 μg/mL B) 25 μg/mL (water solution of laundry detergent powder) 市販クレンザー中の陰イオン界面活性剤 Anionic surfactants in a commercial cleanser 非イオン性界面活性剤 Nonionic surfactants Column : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) containing 0.1M NaClO4 Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : FLS at Ex 221 nm, Em 284 nm Injection : 20 μL (2.0 μg/mL) Column A) Standard C nH 2n+1 mV C8H17 1 300 (OCH2CH2)nOH SO3Na mAU 2 3 200 4 5 100 0 0 N040326B 10 20 1. Sodium decylbenzenesulfonate (n=10) 2. Sodium undecylbenzenesulfonate (n=11) 3. Sodium dodecylbenzenesulfonate (n=12) 4. Sodium tridecylbenzenesulfonate min (n=13) 5. Sodium tetradecylbenzenesulfonate (n=14) Triton X-100 30 11 20 B) Cleanser 3 300 10 200 4 1 100 0 0 0 N040323B 10 20 0 min 10 20 30 min (N040323B) Column : YMC-Pack Pro C18 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) containing 0.1M NaClO4 Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : FLS at Ex 221 nm, Em 284 nm Injection : 20 μL Sample : A) 2.0 μg/mL B) 250 μg/mL (water solution of powder cleanser) (N040308D) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 250 × 4.6 mm I.D. Eluent : THF/acetonitrile/water (10/45/45) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254nm Injection : 10 μL (20 mg/mL) 115 Pesticides, Environmental 2 mV 農薬、環境関連物質 11 Pesticides, Environmental 非イオン性界面活性剤 Nonionic surfactant ポリエチレングリコール Polyethyleneglycols 1.Triton X-100 1.PEG #20,000 2.PEG #6,000 3.PEG #4,000 4.PEG #1,000 5.PEG #300 6.Ethyleneglycol (MW 20,000) (MW 8,400) (MW 3,350) (MW 1,000) (MW 300) (MW 62) (G920109C) (G920123A) Column : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : acetonitrile/water (10/90) Flow rate : 1.0 mL/min Temperature : ambient (23 ℃) Detection : UV at 260 nm, 0.02 AUFS Injection : 15 μL (5.0 mg/mL) Sample : Triton X-100 : YMC-Pack Diol-120 500 × 8.0 mm I.D. Eluent : acetonitrile/water(10/90) Flow rate : 1.0 mL/min Temperature : ambient (22 ℃) Detection : RI, 64 x 10-6 RIU/FS Injection : 100 μL (1.67 mg/mL) アルデヒドおよびケトン類の2,4-DNPH誘導体 2,4-Dinitrophenylhydrazones of aldehydes and ketones アルデヒドおよびケトン類の2,4-DNPH誘導体 2,4-dinitrophenylhydrazones of aldehydes and ketones Column 1.Formaldehyde 2,4-DNPH 2.Acetaldehyde 2,4-DNPH 3.Acetone 2,4-DNPH 4.Acrolein 2,4-DNPH 5.Propionaldehyde 2,4-DNPH 6.Crotonaldehyde 2,4-DNPH 7.Methylethylketone 2,4-DNPH 8.Isobutyraldehyde 2,4-DNPH 9.Benzaldehyde 2,4-DNPH 10.n-Valeraldehyde 2,4-DNPH 11.p-Tolualdehyde 2,4-DNPH 12.Capronaldehyde 2,4-DNPH 1.Formaldehyde 2,4-DNPH 2.Acetaldehyde 2,4-DNPH 3.Acetone 2,4-DNPH 4.Acrolein 2,4-DNPH 5.Propionaldehyde 2,4-DNPH 6.Crotonaldehyde 2,4-DNPH 7.Methylethylketone 2,4-DNPH 8.Isobutyraldehyde 2,4-DNPH 9.Benzaldehyde 2,4-DNPH 10.n-Valeraldehyde 2,4-DNPH 11.p-Tolualdehyde 2,4-DNPH 12.Capronaldehyde 2,4-DNPH 11 農 薬 ・ 環 境 関 連 物 質 (T920302A) (T931130D) Column : YMC-Pack C4 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : A)tetrahydrofuran/water (10/90) B)acetonitrile 35%B (0-7min), 35-65%B (7-18min, linear), 100%B (18-19min), 35%B (19-35min) Flow rate : 1.5 mL/min Temperature : 30 ℃ Detection : UV at 360 nm, 0.01 AUFS Injection : 11 μL (0.0025 mg/mL) 116 Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : A)tetrahydrofuran/water (20/80) B)acetonitrile/methanol (90/10) 30-67%B (0-15min, Linear), 30%B (15-25min) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 360 nm, 0.01 AUFS Injection : 15 μL (0.001 mg/mL) 農薬、環境関連物質 11 Pesticides, Environmental 多環芳香族炭化水素 Polyaromatic hydrocarbons 多環芳香族炭化水素 Polyaromatic hydrocarbons 1.Benzene 2.Naphthalene 3.7,12-Dimethylbenzo[a]anthracene 4.Acenaphthylene 5.Diphenyl 6.Fluorene 7.Naphthacene 8.Acenaphthene 9.Phenanthrene 10.Anthrathene 11.Fluoranthene 12.Pyrene 13.Triphenylene 14.Chrysene 15.Perylene 16.Benzo[e]pyrene 17.Benzo[b]fluoranthene 18.Benzo[k]fluoranthene 19.Benzo[a]pyrene 20.Dibenzo[a,h]anthracene 21.Benzo[g,h,i]perylene 22.Coronene 1.Benzene 2.Naphthalene 3.Acenaphthylene 4.7,12-Dimethylbenzo[a]anthracene 5.Diphenyl 6.Fluorene 7.Acenaphthene 8.Naphthacene 9.Phenanthrene 10.Anthrathene 11.Fluoranthene 12.Pyrene 13.Triphenylene 14.Chrysene 15.Perylene 16.Benzo[e]pyrene 17.Benzo[b]fluoranthene 18.Benzo[k]fluoranthene 19.Benzo[a]pyrene 20.Dibenzo[a,h]anthracene 21.Benzo[g,h,i]perylene 22.Coronene (K930918A) Column : J'sphere ODS-H80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 7 μL (K930918B) Column : J'sphere ODS-M80 (4μm, 8nm) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 7 μL 多環芳香族炭化水素 Polyaromatic hydrocarbons 1.Benzene 2.Naphthalene 3.7,12-Dimethylbenzo[a]anthracene 4.Acenaphthylene 5.Diphenyl 6.Fluorene 7.Naphthacene 8.Acenaphthene 9.Phenanthrene 10.Anthrathene 11.Fluoranthene 12.Pyrene 13.Triphenylene 14.Chrysene 15.Perylene 16.Benzo[e]pyrene 17.Benzo[b]fluoranthene 18.Benzo[k]fluoranthene 19.Benzo[a]pyrene 20.Dibenzo[a,h]anthracene 21.Benzo[g,h,i]perylene 22.Coronene 11 Pesticides, Environmental (K930918C) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : A)acetonitrile/water (50/50) B)acetonitrile 30%B (0-5min), 30-100%B (5-40min), 100%B (40-50min) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 7 μL 117 その他 12 The others ヒドロキシ安息香酸 Hydroxybenzoic acids レゾルシン酸異性体 Resorcylic acid isomers 1 4 7 1 3,5-DHBA 2 2,5-DHBA 5 2,3-DHBA 8 α-Resorcylic acid 2 3,4-DHBA 3 2,4-DHBA 6 Benzoic acid 9 β-Resorcylic acid 3 p-HBA m-HBA Salicylic acid Resorcinol DHBA, Dihydroxybenzoic acid HBA, Hydroxybenzoic acid 4 γ-Resorcylic acid (O940530D) (O940607C) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (5/90/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 285 nm, 0.16 AUFS Injection : 5 μL (0.1 mg/mL) Column : YMC-Pack C8 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.4 mg/mL) レゾルシン酸異性体 Resorcylic acid isomers 安息香酸誘導体 Benzoic acid derivatives 1 1 p-Ethoxybenzoic acid 2 α-Resorcylic acid 2 p-Propoxybenzoic acid 3 β-Resorcylic acid 3 4 Resorcinol p-Isopropylbenzoic acid (Cuminic acid) 4 p-Buthoxybenzoic acid 12 5 γ-Resorcylic acid そ の 他 p-tert-Butylbenzoic acid (O940606J) Column : YMC-Pack Ph (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/100mM KH2PO4 (10/90) Flow rate : 0.8 mL/min Temperature : 37 ℃ Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.4 mg/mL) 118 (O950511B) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (40/60/1) Flow rate : 0.5 mL/min Temperature : ambient (26 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.04∼0.2 mg/mL) その他 12 The others 安息香酸誘導体 Benzoic acid derivatives メトキシ安息香酸異性体 Methoxybenzoic acid isomers 1 1 p-Ethoxybenzoic acid 2 o-Methoxybenzoic acid (o-Anisic acid) 2 p-Propoxybenzoic acid 3 p-Methoxybenzoic acid (p-Anisic acid) 4 p-Isopropylbenzoic acid (Cuminic acid) 3 m-Methoxybenzoic acid (m-Anisic acid) p-Buthoxybenzoic acid 5 p-tert-Butylbenzoic acid (O950511C) (O950508A) Column : J'sphere ODS-L80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (40/60/1) Flow rate : 0.5 mL/min Temperature : ambient (26 ℃) Detection : UV at 260 nm, 0.32 AUFS Injection : 10 μL (0.04∼0.2 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.17 mg/mL) メトキシ安息香酸異性体 Methoxybenzoic acid isomers クロロ安息香酸異性体 Chlorobenzoic acid isomers 1 1 o-Chlorobenzoic acid o-Methoxybenzoic acid (o-Anisic acid) 2 2 m-Chlorobenzoic acid 3 p-Methoxybenzoic acid (p-Anisic acid) 3 p-Chlorobenzoic acid m-Methoxybenzoic acid (m-Anisic acid) 12 Column : J'sphere ODS-M80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (20/80/1) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 285 nm, 0.16 AUFS Injection : 10 μL (0.17 mg/mL) (O950509B) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : UV at 270 nm, 0.04 AUFS Injection : 10 μL (0.17 mg/mL) 119 The others (O950508B) その他 12 The others クロロ安息香酸異性体 Chlorobenzoic acid isomers フタル酸異性体 Phthalic acid isomers 1 1 o-Chlorobenzoic acid 2 Phthalic acid m-Chlorobenzoic acid 2 3 Terephthalic acid p-Chlorobenzoic acid 3 Isophthalic acid (O950509D) (A971112B) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (25/75/1) Flow rate : 1.0 mL/min Temperature : ambient (27 ℃) Detection : UV at 270 nm, 0.04 AUFS Injection : 10 μL (0.17 mg/mL) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 3.0 mm I.D. Eluent : methanol/10mM H3PO4 (25/75) Flow rate : 0.4 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 4 μL (0.05∼0.5 mg/mL) フタル酸異性体 Phthalic acid isomers ポリフェノール Polyphenols 1.Pyrogallol 2.Hydroquinone 3.Catechol 4.Phenol 2 1 1 Phthalic acid 3 2 4 Terephthalic acid 3 12 Isophthalic acid そ の 他 0 10 20min (A971112A) Column : YMC-Pack ODS-AQ (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : methanol/10mM H3PO4 (25/75) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.05∼0.5 mg/mL) 120 (H890627A) Column : YMC-Pack Ph (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 5mM acetic acid Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 280 nm, 0.32 AUFS Injection : 20 μL その他 12 The others ジメチルフェノール異性体 Dimethylphenol isomers ジメチルフェノール異性体 Dimethylphenol isomers 1 2 2,6-Dimethylphenol 3 2,4-Dimethylphenol 5 2,5-Dimethylphenol 4 2 2,6-Dimethylphenol 2,5-Dimethylphenol 4 3 2,4-Dimethylphenol 2,3-Dimethylphenol 5 6 3,5-Dimethylphenol 1 3,4-Dimethylphenol 2,3-Dimethylphenol 6 3,5-Dimethylphenol 3,4-Dimethylphenol (T921019B) Column : YMC-Pack Polyamine II 250 × 4.6 mm I.D. Eluent : 20% ethyl acetate in hexane Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 20 μL (0.33 mg/mL) フェノール誘導体 Phenol derivatives (T921020C) Column : YMC-Pack NH2 (5μm, 12nm) 250 × 4.6 mm I.D. Eluent : 10% ethyl acetate in hexane Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.08 AUFS Injection : 20 μL (0.33 mg/mL) フェノール誘導体 Phenol derivatives 1 1 o-Nitrophenol 2,6-Dinitrophenol 2 2 o-Chlorophenol 2,4,6-Trinitrophenol 3 2,4-Dinitrophenol 4 3 m-Nitrophenol 4 p-Nitrophenol 2,6-Dichlorophenol 5 5 12 m-Chlorophenol 2,4-Dichlorophenol 6 6 (O950616I) Column : J'sphere ODS-H80 (4μm, 8nm) 75 × 4.6 mm I.D. Eluent : 2-propanol/water/TFA (40/60/0.1) Flow rate : 0.4 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.13 AUFS Injection : 5 μL (0.04 mg/mL) (O950614C) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (35/65/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.13 AUFS Injection : 4 μL (0.08 mg/mL) 121 The others p-Chlorophenol 2,4,6-Trichlorophenol その他 12 The others フェノール誘導体 Phenol derivatives クレゾール異性体 Cresol isomers 1 o-Nitrophenol 1 2 m-Cresol o-Chlorophenol 3 2 m-Nitrophenol p-Cresol 4 3 p-Nitrophenol 5 o-Cresol m-Chlorophenol 6 p-Chlorophenol (O950614F) (O951025B) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : tetrahydrofuran/water/acetic acid (35/65/1) Flow rate : 0.5 mL/min Temperature : 37 ℃ Detection : UV at 240 nm, 0.13 AUFS Injection : 4 μL (0.08 mg/mL) Column : J'sphere ODS-H80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (18/82) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 10 μL (0.3 mg/mL) クレゾール異性体 Cresol isomers クロロフェノール異性体 Chlorophenol isomers 1 1. OH Cl m-Cresol 2 o-Chlorophenol 2 2. OH Cl p-Cresol p-Chlorophenol 3 3 3. OH 1 o-Cresol Cl m-Chlorophenol 12 そ の 他 0 10 20 min (J011113D) (O951025C) Column : J'sphere ODS-M80 (4μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (18/82) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 260 nm, 0.064 AUFS Injection : 10 μL (0.3 mg/mL) 122 Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water/acetic acid (30/70/1) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 230 nm, 0.32 AUFS Injection : 10 μL (0.1∼0.2 mg/mL) その他 12 The others クロロアセトフェノン異性体 Chloroacetophenone isomers ターフェニル異性体 Terphenyl isomers 1. 1. COCH3 Cl 2 o-Chloroacetophenone 2 o-Terphenyl 2. COCH3 2. 3 Cl 1 p-Chloroacetophenone 1 m-Terphenyl 3 3. 3. COCH3 Cl p-Terphenyl m-Chloroacetophenone 0 10 20 min 0 10 20 min (J011026B) (J020719E) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : acetonitrile/water (40/60) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm, 0.32 AUFS Injection : 8.0 μL (0.05∼0.1 μL/mL) Column : YMC-Pack Pro C18 RS (5μm, 8nm) 150 × 4.6 mm I.D. Eluent : methanol/water (85/15) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.16 AUFS Injection : 10 μL (0.02 mg/mL) フラーレン同族体 Fullerenes フラーレン同族体 Fullerenes 1 1 Buckminsterfullerene(C60) Buckminsterfullerene(C60) 2 2 (5,6)-Fullerene(C70) (5,6)-Fullerene(C70) 12 Column : YMC-Pack ODS-A (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : hexane/2-propanol (70/30) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 350 nm, 0.08 AUFS Injection : 4 μL (0.125 mg/mL) (S930128B) Column : YMC-Pack Ph (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : hexane/2-propanol (50/50) Flow rate : 1.0 mL/min Temperature : ambient (25 ℃) Detection : UV at 350 nm, 0.08 AUFS Injection : 4 μL (0.125 mg/mL) 123 The others (S930119B) その他 12 The others 1- (2-ピリジルアゾ) -2-ナフトール(PAN)金属キレート Metal-PAN chelates 配位性化合物 Coordination compounds 1.Co (Ⅱ)-1- (2-Pyridylazo)-2-naphthol 2.Ni (Ⅱ)-1- (2-Pyridylazo)-2-naphthol 3.Fe (Ⅲ)-1- (2-Pyridylazo)-2-naphthol 4.Zn (Ⅱ)-1- (2-Pyridylazo)-2-naphthol 1. Uracil 2. Acetylacetone 3. 8-Quinolinol (T910520A) (M910604I) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 6.0 mm I.D. Eluent : acetonitrile/water (70/30) containig 0.01% PAN and 10mM tetrabutylammonium bromide Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : VIS at 565 nm, 0.08 AUFS Injection : 12 μL (25∼92 μg/mL) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM KH2PO4 - K2HPO4 (pH7.6)/methanol (40/60) Flow rate : 1.0 mL/min Temperature : 40 ℃ Detection : UV at 254 nm, 0.32 AUFS ヒノキチオール Hinokitiol アミノアルコール Aminoalcohols 1.Ethanolamine 2.Diethanolamine 3.N-Methylethanolamine 4.Triethanolamine 5.N,N-Dimethylethanolamine 1 Hinokitiol 2 1-Naphthol(I.S.) 12 そ の 他 (O960507F) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 20mM NH4H2PO4 containing 1mM EDTA･2Na/acetonitrile (65/35) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 320 nm, 0.16 AUFS Injection : 10 μL (0.08, 0.25 mg/mL) 124 (M920124B) Column : YMC-Pack PolymerC18 250 × 6.0 mm I.D. Eluent : 100mM Na2HPO4/100mM NaOH (60/40, pH 12.0) Flow rate : 0.6 mL/min Temperature : 20 ℃ Detection : UV at 215 nm, 0.32 AUFS Injection : 50 μL (0.2∼3.0 mg/mL) その他 12 The others 尿素及び関連化合物 Urea and related compounds 芳香族アミン Aromatic amines 1.Urea 2.Propionamide 3.Ethylurea 4.1,3-Diethylurea 5.1,1-Diethylurea 1 p-Phenylenediamine 2 p-Aminophenol 3 m-Phenylenediamine 4 m-Aminophenol 5 o-Phenylenediamine 6 o-Aminophenol (M920108A) Column : YMC-Pack PolymerC18 250 × 6.0 mm I.D. Eluent : 0.4% HClO4 (pH 1.3) Flow rate : 0.5 mL/min Temperature : 25 ℃ Detection : UV at 210 nm, 0.32 AUFS Injection : 15 μL (0.4∼7.5 mg/mL) 芳香族アミン Aromatic amines (S961007C) Column : YMC-Pack Pro C18 (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/methanol (95/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.26 AUFS Injection : 5 μL (0.125∼0.5 mg/mL) 1 p-Phenylenediamine 2 p-Aminophenol 3 m-Phenylenediamine 4 m-Aminophenol 5 12 o-Phenylenediamine The others 6 o-Aminophenol (S961004E) Column : YMC-Pack ODS-AM (5μm, 12nm) 150 × 4.6 mm I.D. Eluent : 50mM NH4H2PO4 - (NH4)2HPO4 (pH 6.7)/methanol (95/5) Flow rate : 1.0 mL/min Temperature : 37 ℃ Detection : UV at 254 nm, 0.26 AUFS Injection : 5 μL (0.125∼0.5 mg/mL) 125 光学異性体 13 Optical isomers ビナフトール 1,1'-Bi-2-naphthol トリフルオロアンスリルエタノール 2,2,2-Trifluoro-1-(9-anthryl)-ethanol (N020605B) (N020605A) Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (50/50) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 235 nm Injection : 1 μL (2.8 mg/mL) Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (40/60) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 254 nm Injection : 1 μL (0.14 mg/mL) ヘキソバルビタール Hexobarbital CBZ-フェニルアラニン CBZ-Phenylalanine(Z-Phe-OH) 13 (N020605D) (N020605C) 光 学 異 性 体 Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/water (30/70) Flow rate : 0.7 mL/min Temperature : ambient Detection : UV at 210 nm Injection : 1 μL (1.2 mg/mL) 126 Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : 0.5M NaClO4 - HClO4 (pH 2.0)/acetonitrile (70/30) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 254 nm Injection : 10 μL (1.5 mg/mL) 光学異性体 13 Optical isomers 塩酸プロプラノロール Propranolol hydrochloride 塩酸ケタミン Ketamine hydrochloride (N020605F) (N020605E) Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/0.5M NaClO4 (40/60) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 254 nm Injection : 5 μL (1.5 mg/mL) Column : YMC CHIRAL NEA (R) 250 × 4.6 mm I.D. Eluent : acetonitrile/0.5M NaClO4 (40/60) Flow rate : 1.0 mL/min Temperature : ambient Detection : UV at 268 nm Injection : 10 μL (1.4 mg/mL) クロルタリドン Chlorthalidone フェノプロフェンカルシウム Fenoprofen calcium salt; hydrate (N020605H) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (10/90) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : water/acetic acid (100/1) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) Column 13 Optical isomers (N020605G) 127 光学異性体 13 Optical isomers フルルビプロフェン Flurbiprofen ヘキソバルビタール Hexobarbital (N020605I) Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (10/90) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (70/30) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) イブプロフェン Ibuprofen インダパミド Indapamide 13 Column (N020605K) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.1M AcOHaq - TEAaq (pH 4.0)/acetonitrile (10/90) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 20 μL (2 mg/mL) Column 光 学 異 性 体 (N020605J) 128 (N020605L) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/acetonitrile (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) Column 光学異性体 13 Optical isomers ケトプロフェン Ketoprofen CBZ-フェニルアラニン CBZ-Phenylalanine (Z-Phe-OH) (N020605N) (N020605M) Column : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (30/70) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 10 μL (1 mg/mL) : YMC CHIRAL β-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (40/60) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) フルルビプロフェン Flurbiprofen ナフチルエチルアルコール 1-(1-Naphthyl)-ethylalcohol Column (N020605P) (N020605O) Optical isomers : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/methanol (30/70) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 2 μL (1 mg/mL) Column 13 : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/methanol (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column 129 光学異性体 13 Optical isomers フェニルエチルアルコール Phenylethylalcohol 塩酸プロプラノロール Propranolol hydrochloride (N020605Q) Column : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/methanol (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (10 mg/mL) : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : acetonitrile/methanol/acetic acid/triethylamine (99/1/0.3/0.25) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) トレーガー塩基 Troger's base CBZ-フェニルアラニン CBZ-Phenylalanine (Z-Phe-OH) 13 Column (N020605T) (N020605S) : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.2M AcOH - 0.2M TEA (pH 5.6)/acetonitrile (90/10) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 30 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column 光 学 異 性 体 (N020605R) 130 : YMC CHIRAL γ-CD BR 250 × 4.6 mm I.D. Eluent : 0.53M AcOH - 0.53M TEA (pH 4.0)/acetonitrile (60/40) (TEA=triethylamine) Flow rate : 1.0 mL/min Temperature : 25 ℃ Detection : UV at 254 nm Injection : 5 μL (1 mg/mL) Column APPLICATION DATA COLLECTIONS 化合物名インデックス Index of Analytes 131 化合物名インデックス Index of Analytes A Acacetin Acenaphthene Acenaphthylene Acetaldehyde 2,4-DNPH Acetaminophen Acetic acid Acetoacetyl-CoA Acetone 2,4-DNPH Acetylacetone 5-Acetylamino-6-amino-3-methyluracil N-Acetyl-chitobiose N-Acetyl-chitopentaose N-Acetyl-chitotetraose N-Acetyl-chitotriose Acetyl-CoA N-Acetylglucosamine N-Acetyl procainamide Acid red Acrolein 2,4-DNPH Acyclovir Adenine Adenosine Adenylate kinase 5'-ADP ADP-D-glucose Aflatoxin B1 Aflatoxin B2 Aflatoxin G1 Aflatoxin G2 Alachlor Alanine L-Alanine Albiflorin Aldicarb Allantoic acid Allantoin Allopurinol Amaranth Ametryn p -Aminobenzoic acid (PABA) o -Aminophenol m -Aminophenol p -Aminophenol Aminopterin Amitriptyline hydrochloride Amoxicillin 5'-AMP Ampicillin Amyloid β-protein Androsterone trans -Androsterone Angiotensin I [Asn1,Val5] Angiotensin I [Val5] Angiotensin I Angiotensin II Angiotensin III o -Anisic acid m -Anisic acid p -Anisic acid Annatto extract 132 64 88, 89, 117 117 116 81, 82, 83 52 18 116 124 97 28 28 28 28 18 28 96 57 116 96 34 34, 35 50, 51 35 37 69 69 69 69 113 39 39, 40 62 114 98 17, 98 97 57 112 14, 15, 17 125 125 125 94 88, 89 109, 110 35, 36 102, 109 45 75 75 41, 42, 43 41 41 41, 43, 44, 49 41, 44, 45 119 119 119 58 Anthrathene Apigenin Apronalide Arabitol Arachidonic acid Arbutin Arginine L-Arginine L-Ascorbic acid 117 64 83 21 53 61, 68 39 39, 40 13, 15, 16, 17, 19, 81 19 39 40 58, 59 39 39, 40 111, 112 35 112 112, 113 112 112 112 60 94, 95 L-Ascorbic acid 2,4-DNPH Asparagine L-Asparagine Aspartame Aspartic acid L-Aspartic acid Asulam 5'-ATP Atraton Atrazine Atrazine-desethyl Atrazine-desisopropyl Atrazine-2-hydroxy Atropine sulfate AZT B Bacampicillin hydrochloride Baicalein Baicalin BAM-12P Barbaloin Beclomethasone Beclomethasone dipropionate Bendiocarb Benfotiamine Bensulide Benzaldehyde 2,4-DNPH Benzalkonium chloride Benzene Benzethonium chloride Benzo[a]pyrene Benzo[b]fluoranthene Benzo[e]pyrene Benzo[g,h,i]perylene Benzo[k]fluoranthene Benzocaine Benzoic acid Benzylpenicillin potassium Bepridil hydrochloride Betamethasone Betamethasone valerate Bezafibrate BHA (Butyl hydroxyanisol) BHT (Dibutyl hydroxytoluene) 1,1'-Bi-2-naphthol Biotin Bisbentiamine Bombesin Bradykinin Brassicasterol 109 64 61, 65 43 61 72, 74 74 114 16, 17, 82 111, 112 116 85, 86 71, 117 84, 85, 86 117 117 117 117 117 93 54, 58, 59, 96, 118 109 91, 92 72, 74 74 90 56, 57 56, 57 126 14, 15 17 42 42, 43, 44, 45 78 INDEX Brilliant Blue FCF Bromovalerylurea BSA Bucetine Bufalin Butacaine Butachlor p -Buthoxybenzoic acid p -tert-Butylbenzoic acid tert -Butylhydroquinone (TBHQ) Butyl hydroxyanisol (BHA) Butyl p-hydroxybenzoate 57 83, 84 45, 46 82 80 93 113 118, 119 118, 119 55, 56 56, 57 55, 68, 69, 83, 87 C Caffeine Campesterol Capronaldehyde 2,4-DNPH Capsaicin Carbadox Carbamazepine Carbendazim Carnitine chloride α-Carotene β-Carotene Casein (+)-Catechin Catechol CBZ-Phenylalanine (Z-Phe-OH) CCK-Octapeptide 5'-CDP CDP-D-glucose Cefaclor Cefadroxil Cefamandole sodium Cefatrizine propylene glycol Cefazolin sodium Cefoperazone sodium Cefotaxime sodium Cefoxitin sodium Cefsulodin sodium Ceftriaxone sodium Cefuroxime sodium Cephalexin Cephaloglycin Cephaloridine Cephalosporin C potassium Cephalothin sodium Cephapirin sodium Cephradine Chenodeoxycholic acid (CDAC) Chloramphenichol Chlorhexidine hydrochloride o -Chloroacetophenone m -Chloroacetophenone p -Chloroacetophenone o -Chlorobenzoic acid m -Chlorobenzoic acid p -Chlorobenzoic acid 13, 14, 58, 59, 61, 81, 82, 83, 84, 86, 97, 108, 109 78 116 70 100 89 112 96 8 8, 9 50 66 120 126, 129, 130 44 35 37 103, 104, 107 104, 105 105, 106 104, 105 103, 104 105, 106 103, 104, 106, 107 103, 104, 108, 109 103, 104 106, 107, 108 103, 104 102, 103, 104, 105, 107, 110 103, 104, 107 103, 104, 105, 107 104, 105, 108 105, 106 103, 104, 107 103, 104 77 102 86 123 123 123 119, 120 119, 120 83, 119, 120 o -Chlorophenol m -Chlorophenol p -Chlorophenol Chlorophyll a Chlorophyll b Chloropropham Chlorotetracycline Chlorpheniramine maleate Chlorpromazine hydrochloride Chlorthalidone Cholecalciferol Cholesterol Cholic acid (CA) Chondroitin, Na salt Chondroitin sulfate A, Na salt Chondroitin sulfate B, Na salt Chondroitin sulfate C, Na salt Chrysene Chrysin Chrysoeriol α-Chymotrypsinogen A Cinchonidine Cinchonine Cinobufagin Cinoxacin Citric acid Clofibrate Cloxacillin sodium 5'-CMP Codeine Coenzyme A Coenzyme A glutathione disulfide Coenzyme A oxidized Color additives (Acid red) Color additives (Amaranth) Color additives (Brilliant Blue FCF) Color additives (Erythrosine) Color additives (Fast Green FCF) Color additives (Indigo Carmine) Color additives (New Coccine) Color additives (Phloxine) Color additives (Rose Bengale) Color additives (Sunset Yellow FCF) Color additives (Tartrazine) Conalbumin Copper 8-quinolinolate Coronene Corticosterone Corticosterone acetate Cortisone Cortisone acetate o -Cresol m -Cresol p -Cresol Crimidine Crotonaldehyde 2,4-DNPH 5'-CTP Cuminic acid Cyanazine Cyanocobalamin α-Cyclodextrin β-Cyclodextrin 121, 122 121, 122 121, 122 69 69 113 102 81, 82, 84, 85, 86, 87 89 93, 127 8, 9, 10 78 77 31 31 31 31 117 64 64 46 60 60, 93 80 102, 103 52 90 109 35, 36 84 18 18 18 57 57 57 57 57 57 57 57 57 57 57 46 111, 112 117 71, 72, 74 71, 74 71, 72, 74 71, 74 122 122 122 112 116 35 118, 119 112, 113 13, 14, 15, 16 26, 27 26, 27 133 化合物名インデックス γ-Cyclodextrin L-Cystine Cytidine Cytochrome c Cytochrome c (dimer) Cytochrome c (hexamer) Cytochrome c (tetramer) Cytochrome c (trimer) Cytocine Index of Analytes 26, 27 39, 40 34, 35 45, 46, 48, 50, 51 48 48 48 48 34 D D4T Daidzein Daidzin 5'-dAMP 5'-dCMP DDC DDI Dehydroacetic acid Dehydroisoandrosterone Deoxyadenosine Deoxycorticosterone Deoxycorticosterone acetate Deoxycytidine Deoxyguanosine Deoxyinosine Deoxyuridine Desipramine hydrochloride Dexamethasone Dextromethorphan hydrobromide 5'-dGMP ΔDi-0S ΔDi-4S ΔDi-6S ΔDi-diSD ΔDi-diSE ΔDi-triS Diaveridine Dibenzo[a,h]anthracene Dibutyl hydroxytoluene (BHT) Dicethiamine hydrochloride 2,4-Dichlorophenol 2,6-Dichlorophenol Dicloxacillin sodium Diethanolamine 1,1-Diethylurea 1,3-Diethylurea Digitoxigenin Digitoxin Digoxigenin Digoxigenin-bis-digitoxoside Digoxin Dihydrocapsaicin Dihydrocodeine phosphate Dihydroquinidine Dihydroquinine 2,3-Dihydroxybenzoic acid 2,4-Dihydroxybenzoic acid 2,5-Dihydroxybenzoic acid 3,4-Dihydroxybenzoic acid 3,5-Dihydroxybenzoic acid 134 94, 95 66 66 35 35 94, 95 94, 95 54 75 34, 35 71, 72, 73, 74 74 34, 35 34, 35 34, 35 94 88, 89 72, 74 81 35 21 21 21 21 21 21 100 117 56, 57 17 121 121 109 124 125 125 79 61, 79 79 79 79 70 81, 82 60 60 118 118 118 118 118 3,4-Dihydroxyphenylacetic acid 3,4-Dihydroxyphenylalanine (DOPA) Diltiazem hydrochloride 7,12-Dimethylbenzo[a]anthracene O -Dimethyl-β-cyclodextrin N,N -Dimethylethanolamine 2,3-Dimethylphenol 2,4-Dimethylphenol 2,5-Dimethylphenol 2,6-Dimethylphenol 3,4-Dimethylphenol 3,5-Dimethylphenol 1,7-Dimethyluric acid 1,9-Dimethyluric acid 2,4-Dinitrophenol 2,6-Dinitrophenol Diphenhydramine hydrochloride Diphenyl Diuron Docosahexaenoic acid n -Dodecyl gallate (DG) DOPA (3,4-Dihydroxyphenylalanine) Dopamine hydrochloride Doxepin hydrochloride Doxorubicin hydrochloride Dynorphin A (Human,1-13) 98 98 91, 92 117 27, 28 124 121 121 121 121 121 121 97 97 121 121 87 77, 114, 117 112, 113 53 55, 56 98 98 88 102 42 E α-Ecdysone β-Ecdysone Cu(II)-EDTA Fe(III)-EDTA Eicosapentaenoic acid Eicosatrienoic acid Eledoisin related peptide α-Endorphin β-Endorphin (Human) γ-Endorphin [D-Ala2,Met5]-Enkephalin Leu-Enkephalin Met-Enkephalin [D-Ala2,Met5]-Enkephalinamide Enolase Enoxacin (-)-Epicatechin (-)-Epicatechin gallate (-)-Epigallocatechin (-)-Epigallocatechin gallate Epinephrine Ergocalciferol Erythorbic acid Erythritol Erythromycin Erythromycin estolate Erythromycin ethylsuccinate Erythrosine Esculine β-Estradiol Estradiol benzoate Estradiol dipropionate Estriol 78 78 55 55 53 53 42 43 43 43 43 42, 43, 44, 45 42, 43, 44, 45 43 50, 51 102 66 66 66 66 98 8, 9, 10 16, 19 21, 22 110 110 110 57 61 76, 77 76, 77 76, 77 76 INDEX Estrone Ethanolamine Ethenzamide Ethinylestradiol Ethiofencarb Ethopropazine hydrochloride Ethosuximide p -Ethoxybenzoic acid Ethyl 4-aminobenzoate Ethylbenzene Ethyleneglycol Ethyl p-hydroxybenzoate Ethylurea 76 124 81, 82 , 83, 84 76, 77 114 89 89 118, 119 93 74 116 55 125 F Fast Green FCF Fenofibrate Fenoprofen Flavin adenine dinucleotide disodium Flavin mononucleotide sodium salt (Flavin sodium phosphate) Fluocinolone acetonide Fluocinonide Fluoranthene Fluorene 5-Fluorocytosine 5-Fluoro-2'-deoxyuridine 5-Fluoroorotic acid 5-Fluorouracil Fluoxymesterone Fluphenazine hydrochloride Flurbiprofen Folic acid Formaldehyde 2,4-DNPH 1-Fructofuranosyl-D-nystose Fructose Fucose Fucosterol Fullerene (C60) Fullerene (C70) Fumaric acid Furazolidone Furosemide 57 90 87, 88, 127 18 13, 14, 18 71, 72, 73, 74 72, 73, 74 117 117 34 94 35 34, 94 74, 75 89 87, 88, 91, 128, 129 13, 94 116 23 20, 22 20 78 123 123 52, 82 100 93 Glutamine Glutathione (GSH) Glutathione disulfide (GSSG) Glycerol Glycine Glycolic acid Glycyrrhizic acid (Glycyrrhizin) 5'-GMP 5'-GTP Guanine Guanosine H Heparan sulfate, Na salt Heparin, Na salt Hesperidin Hexazinone Hexobarbital Hinokitiol Hippuric acid Histidine L-Histidine Homovanillic acid Hordenine sulfate Human IgA Human IgG Human IgM(κ), myeloma protein Hyaluronic acid, Na salt Hydrochlorothiazide Hydrocortisone Hydrocortisone acetate Hydrocortisone succinate Hydroquinone 4'-Hydroxyacetophenone(p-) m -Hydroxybenzoic acid p -Hydroxybenzoic acid 4-Hydroxy-3-methoxymandelic acid 4-Hydroxy-3-methoxyphenylglycol 4-Hydroxymethyl-2,6-di-tert-butylphenol (HMBP) 3-Hydroxy-3-methylglutaryl-CoA (HMG-CoA) Hyodeoxycholic acid (HDCA) Hypoxanthine G Galactose Gallic acid 5'-GDP GDP-D-mannose Gemfibrozil Genistein Genistin Ginsenoside-Rb1 Ginsenoside-Rg1 Glucose Glutamate dehydrogenase Glutamic acid L-Glutamic acid 39 40 40 21 39, 40, 49 52 59, 61, 86 35, 36 35 34 34, 35 32 32 65 112 126, 128 124 98, 99 39 39, 40, 96 98 60 46, 47 46, 47, 48 46, 47, 48 32 93 71, 72, 74 71, 74 73 68, 120 62, 96, 110 118 118 98 98 55, 56 18 77 34, 97 I 20 68 35 37 90 66 66 63, 64 63, 64 20, 22, 24, 26 50, 51 39 39, 40 Ibuprofen Imazalil Imipramine hydrochloride 5'-IMP Indapamide Indigo Carmine Indometacin (Indomethacin) Inosine Inositol Insulin Insulin (Bovine) Insulin (Equine) Insulin (Human) 81, 84, 87, 88, 128 114 88, 89 35, 36 128 57 80, 83 34, 35 21 42, 45 41, 42, 45, 49 41, 42 41, 42 135 化合物名インデックス Insulin (Porcine) Insulin (Sheep) Insulin chain B Iprodion Isobutyl p-hydroxybenzoate Isobutyraldehyde 2,4-DNPH Isoleucine L-Isoleucine Isomalt Isonicotinic acid Isonicotinic acid hydrazide Isophthalic acid p -Isopropylbenzoic acid Isopropyl p-hydroxybenzoate Isoproturon Isoquercitrin Index of Analytes 41, 42 41, 42 44 112 55 116 39 39, 40 21 95 95 120 118, 119 55 112, 113 65 K Kaempferol Keratan polysulfate, Na salt Keratan sulfate, Na salt 1-Kestose Ketamine hydrochloride Ketoprofen 64 33 32 22, 23 127 87, 88, 129 L α-Lactalbumin Lactate dehydrogenase Lactic acid Lactitol β-Lactoglobulin A β-Lactoglobulin B Lactose Lactosyl fructoside Lanatoside B Lanatoside C Leucine L-Leucine Lidocaine Linoleic acid γ-Linolenic acid Linuron Lipoxidase Lovastatin Luteolin Lysine L-Lysine Lysozyme 45 50, 51 52 21, 22 46 46 20 23, 24 79 79 39 39, 40 84, 91, 92, 93 53 53 112, 113 46 90 64 39 39, 40 45, 46 Maltohexaose (G6) Maltopentadecaose (G15) Maltopentaose (G5) Maltose (G2) Maltotetradecaose (G14) Maltotetraose (G4) Maltotridecaose (G13) Maltotriose (G3) Maltoundecaose (G11) Mandelic acid Mannitol Mannose α-Mating factor Mecoprop Medroxyprogesterone acetate Menadione Menaquinone Mestranol Metazachlor Methicillin sodium Methionine L-Methionine Methotrexate o -Methoxybenzoic acid m -Methoxybenzoic acid p -Methoxybenzoic acid 3-Methoxytyramine hydrochloride Methoxyverapamil hydrochloride Methyl benzoate dl-Methylephedrine hydrochloride N -Methylethanolamine Methylethylketone 2,4-DNPH o -Methylhippuric acid m -Methylhippuric acid p -Methylhippuric acid Methyl p-hydroxybenzoate Methyl linoleate Methyl linolenate Methyl γ-linolenate Methylmalonyl-CoA N' -Methylnicotinamide Methyl oleate Methylprednisolone Methyltestosterone 1-Methyluric acid 1-Methylxanthine Metobromuron Metolachlor Metoxuron Myoglobin Peptide fragments from myoglobin Myricetin M Maleic acid L-Malic acid Malonic acid Malonyl-CoA Maltitol Maltododecaose (G12) Maltoheptaose (G7) 136 24 25, 26 24, 26 20, 22, 24 25, 26 24 25, 26 24, 26 25, 26 98, 99 21 20 42, 45 112 76 8 8, 12 76 112, 113 109 39 39, 40 94 119 119 119 98 91, 92 86, 91 81, 82 124 116 98, 99 98, 99 98, 99 55, 65, 84 53 53 53 18 95 53 71, 72, 74 75 97 97 112, 113 112, 113 112, 113 45, 46 48 64 N 52, 81, 84, 86 52 52 18 21, 22 25, 26 24, 26 NAD NADH NADP NADPH Nalidixic acid Naphazoline hydrochloride Naphthacene 18 18 18 18 100, 102, 103 84, 85, 86 117 INDEX Naphthalene 1-Naphthol 1-(1-Naphthyl)-ethylalcohol Naproxen Narcotine Naringin Narirutin Neohesperidin Neostigmine methylsulfate Neurotensin New Coccine Nicardipine hydrochloride Nicotinamide β-Nicotinamide adenine dinucleotide Nicotinamide adenine dinucleotide phosphate oxidized form Nicotinamide adenine dinucleotide phosphate reduced form β-Nicotinamide adenine dinucleotide reduced form Nicotinic acid Nifedipine Niguldipine hydrochloride o -Nitrophenol m -Nitrophenol p -Nitrophenol Nordihydrocapsaicin Nordihydroguaiaretic acid (NDGA) Norepinephrine hydrochloride Norethisterone Norfloxacin Norgestrel Noscapine Nystose 117 124 129 87 60, 82 65 65 65 86 42, 43, 49 57 91, 92 13, 14, 15, 16, 17, 18, 95 18 18 18 18 13, 16, 17, 18, 95 91, 92 91, 92 121, 122 121, 122 121, 122 70 55, 56 98 76 102 77 60, 82 22, 23 O n -Octyl gallate (OG) Ofloxacin Olaquindox Oligonucleotides (c-Ha-ras/12) Orotic acid Ouabagenin Ouabain Ovalbumin Oxamyl Oxine-copper Oxipurinol Oxolinic acid Oxytetracycline hydrochloride Oxytocin 55, 56 102 100 36 15, 35 79 61, 79 45, 46 114 111, 112 97 100, 102, 103 102, 110 42, 43 P Paeoniflorin Paeonol Palatinit Palatinose Pantothenic acid, Na salt Pantothenyl alcohol (Panthenol) 62 68, 69 21 21, 22 13 13, 17, 18 Papaverine chloride Paraxanthine PA-Sugar Chain 001 PA-Sugar Chain 002 PA-Sugar Chain 014 PEG #300 (MW 300) PEG #1,000 (MW 1,000) PEG #4,000 (MW 3,350) PEG #6,000 (MW 8,400) PEG #20,000 (MW 20,000) Penicillin G potassium Penicillin V potassium Pentaglycine Perphenazine Perylene Phenanthrene Phenethicillin potassium Phenobarbital sodium salt Phenol Phenoxyethylpenicillin potassium Phenoxymethylpenicillin potassium Phenylalanine L-Phenylalanine o -Phenylenediamine m -Phenylenediamine p -Phenylenediamine Phenylephrine hydrochloride Phenylethylalcohol 2-Phenylethyl glycoside o -Phenylphenol Phenylpropanolamine hydrochloride Phenytoin Phloxine Phosphoenolpyruvic acid monopotassium salt Phthalic acid Phylloquinone Pilocarpine chloride Pipemidic acid Pirimicarb Piromidic acid Piroxicam Plasmid pBR322 Poly (A) Polydextrose II Potassium guaiacol sulfonate Prednisolone Prednisolone acetate Prednisolone succinate Prednisone Prenylamine lactate Primidone Procainamide hydrochloride Procaine hydrochloride Prochlorperazine maleate Profenamine hydrochloride Progesterone Proline L-Proline Promazine hydrochloride Prometon Prometryn Propazine 60 97 28, 29 28, 29 28, 29 116 116 116 116 116 109 109 49 89 117 117 109 89 61, 96, 108, 120 109 109 39 39, 40 125 125 125 86 130 67 114 86 89, 92 57 52 120 8, 12 60 102 114 100, 102, 103 87 38 37 29 82 71, 73, 74 72, 73, 74 73 71, 72, 74 91, 92 89 96 93 89 89 71, 76 39 39, 40 89 112, 113 112, 113 112, 113 137 化合物名インデックス Propham Propicillin potassium Propionaldehyde 2,4-DNPH Propionamide Propionic acid Propionyl-CoA p -Propoxybenzoic acid Propranolol hydrochloride n -Propyl gallate (PG) Propyl p-hydroxybenzoate Proscillaridin A Pullulan P-5 (MW 5,800) Pullulan P-20 (MW 23,700) Pullulan P-50 (MW 48,000) Pullulan P-800 (MW 853,000) Pyrene Pyridoxal hydrochloride Pyridoxine hydrochloride Co(II)-1-(2-Pyridylazo)-2-naphthol Fe(III)-1-(2-Pyridylazo)-2-naphthol Ni(II)-1-(2-Pyridylazo)-2-naphthol Zn(II)-1-(2-Pyridylazo)-2-naphthol Pyrimethamine Pyrogallol Pyrroloquinoline quinone (PQQ) Pyruvic acid Index of Analytes 113 109 116 125 52 18 118, 119 92, 127, 130 55, 56 55, 59 79 30 30 30 30 117 16, 17 13, 14, 15, 16, 17, 86 124 124 124 124 100 120 15 52 Q Quercetin Quercitrin Quinidine Quinine 8-Quinolinol 64, 65 65 60, 92 60 124 R Raffinose Rebaudioside A Resibufogenin Resorcinol α-Resorcylic acid β-Resorcylic acid γ-Resorcylic acid Retinol Retinol acetate Retinol palmitate Rhamnose Ribitol Riboflavin 23 59 80 18, 108, 118 118 118 118 8 8 8 20 21 13, 14, 15, 16, 17, 18 45, 46 57 65 Ribonuclease A Rose Bengale Rutin S Saccharin sodium Saikosaponin a 138 54, 81 63 Saikosaponin b2 Saikosaponin c Saikosaponin d Salicin Salicylic acid Saponin Sccinyl-CoA Scopolamine bromide Sebuthylazine Sennoside A Sennoside B Serine L-Serine Siduron Simazine β-Sitosterol Sodium decylbenzenesulfonate Sodium dodecylbenzenesulfonate Sodium tetradecylbenzenesulfonate Sodium tridecylbenzenesulfonate Sodium undecylbenzenesulfonate Sorbic acid Sorbitol Sparteine sulfate Stachyose Stevioside Stigmasterol G-Strophanthin Substance P Succinic acid Sucralose Sucrose Sulfachloropyridazine Sulfadiazine Sulfadimethoxine Sulfadimidine Sulfadoxine Sulfaguanidine Sulfamerazine Sulfamethazine Sulfamethizole Sulfamethoxazole Sulfamethoxypyridazine Sulfamonomethoxine Sulfanilamide Sulfapyridine Sulfaquinoxaline Sulfathiazole Sulfisomidine Sulfisoxazole Sulindac Sunset Yellow FCF Swep 63 63 63 61 96, 118 62 18 60 112, 113 67 67 39 39, 40 112 112, 113 78 115 115 115 115 115 54 21, 22 60 23 59 78 61, 79 42 52 22 20, 22, 23 101 100, 101, 102 100, 101 100, 101, 102 101 100, 101 100, 101, 102 100, 101, 102 100, 101 101, 102 100, 101 100, 101, 102 100, 101 100, 101 100, 101 100, 101, 102 100, 101 101 87 57 113 T Tartaric acid Tartrazine Taurine 5'-TDP Terbumeton Terbuthylazine 52 57 39,40 35 112,113 112,113 INDEX Terbutryn Terephthalic acid o-Terphenyl m-Terphenyl p-Terphenyl Testosterone Testosterone enanthate Testosterone propionate Tetracaine hydrochloride Tetracycline hydrochloride Tetrahydrozoline Theobromine Theophylline Thiabendazole Thiamine hydrochloride Thiamphenicol Thiophanate-methyl Thioridazine hydrochloride Thiram Threonine L-Threonine Δ-Threonine Thymidine Thymine 5'-TMP α-Tocopherol β-Tocopherol γ-Tocopherol δ-Tocopherol α-Tocopherol acetate Tolmetin sodium p -Tolualdehyde 2,4-DNPH Triacylglycerol Triamcinolone Triamcinolone acetonide 2,4,6-Trichlorophenol Triethanolamine Trifluoperazine hydrochloride 2,2,2-Trifluoro-1-(9-anthryl)-ethanol Triglycine Trigonelline 2,4,5-Trihydroxybutyrophenone(THBP) Trimethoprim O -Trimethyl-β-cyclodextrin 2,4,6-Trinitrophenol Triphenylene Triton X-100 Troger's base Tryptic digest of synthetic pCDD 88 Tryptic digest of synthetic pCDD 126 Tryptophan L-Tryptophan 5'-TTP Tyrosine L-Tyrosine 112,113 120 123 123 123 75, 76 75, 76 75, 76 93 102, 110 84 61, 97 61, 96, 97 114 13, 14, 17, 81 100 112 89 111, 112 39 39, 40 39 34, 35 34 35, 36 8, 10, 11, 12 10, 11, 12 10, 11, 12 8, 10, 11, 12 8, 12 87 116 53 73 72, 73, 74 121 124 89 126 49 61 55, 56 100, 101 27, 28 121 117 115, 116 130 40 40 39 39 35 39 39, 40 Uracil Urea Uric acid Uridine 5'-UTP 34, 35, 94, 96, 124 17, 125 97, 98 34, 35 35 V n -Valeraldehyde 2,4-DNPH Valine L-Valine Vancomycin hydrochloride [Arg8]-Vasopressin Verapamil hydrochloride Vitamin A Vitamin A palmitate Vitamin B1 Vitamin B2 (Flavin mononucleotide) Vitamin B2 (Riboflavin) Vitamin B6 (Pyridoxal hydrochloride) Vitamin B6 (Pyridoxine hydrochloride) Vitamin B12 Vitamin B13 Vitamin C Vitamin D2 Vitamin D3 Vitamin E Vitamin H Vitamin K1 Vitamin K2 Vitamin K3 116 39 39, 40 110 43 91, 92 8 8 13, 14, 17 13, 14, 18 13, 14, 15, 16, 17, 18 17 13, 14, 15, 16, 17, 86 13, 14, 15, 16 15, 35 13, 15, 16, 17, 19 8, 9, 10 8, 9, 10 8 14, 15 8, 12 8, 12 8 X Xanthine Xanthosine Xylitol Xylose 16, 34, 97 34, 35 21, 22 20 U 5'-UDP UDP-N-acetyl-D-glucosamine UDP-D-glucose 5'-UMP 35 37 37 35, 36 AUTHORIZED DISTRIBUTOR 139 M E M O M E M O M E M O M E M O