The Citric Acid Cycle Is a Source of Biosynthetic Precursors

II. Transducing and Storing Energy
17. The Citric Acid Cycle
17.3. The Citric Acid Cycle Is a Source of Biosynthetic Precursors
Thus far, discussion has focused on the citric acid cycle as the major degradative pathway for the generation of ATP. As
a major metabolic hub of the cell, the citric acid cycle also provides intermediates for biosyntheses (Figure 17.19). For
example, most of the carbon atoms in porphyrins come from succinyl CoA. Many of the amino acids are derived from α ketoglutarate and oxaloacetate. These biosynthetic processes will be discussed in subsequent chapters.
17.3.1. The Citric Acid Cycle Must Be Capable of Being Rapidly Replenished
The important point now is that citric acid cycle intermediates must be replenished if any are drawn off for biosyntheses.
Suppose that much oxaloacetate is converted into amino acids for protein synthesis and, subsequently, the energy needs
of the cell rise. The citric acid cycle will operate to a reduced extent unless new oxaloacetate is formed, because acetyl
CoA cannot enter the cycle unless it condenses with oxaloacetate. Even though oxaloacetate is recycled, a minimal level
must be maintained to allow the cycle to function.
How is oxaloacetate replenished? Mammals lack the enzymes for the net conversion of acetyl CoA into oxaloacetate or
any other citric acid cycle intermediate. Rather, oxaloacetate is formed by the carboxylation of pyruvate, in a reaction
catalyzed by the biotin-dependent enzyme pyruvate carboxylase.
Recall that this enzyme plays a crucial role in gluconeogenesis (Section 16.3.2). It is active only in the presence of acetyl
CoA, which signifies the need for more oxaloacetate. If the energy charge is high, oxaloacetate is converted into glucose.
If the energy charge is low, oxaloacetate replenishes the citric acid cycle. The synthesis of oxaloacetate by the
carboxylation of pyruvate is an example of an anaplerotic reaction (of Greek origin, meaning to "fill up"), a reaction that
leads to the net synthesis, or replenishment, of pathway components. Note that, because the citric acid cycle is a cycle, it
can be replenished by the generation of any of the intermediates.
17.3.2. The Disruption of Pyruvate Metabolism Is the Cause of Beriberi and Poisoning
by Mercury and Arsenic
Beriberi, a neurologic and cardiovascular disorder, is caused by a dietary deficiency of thiamine (also called
vitamin B ). The disease has been and continues to be a serious health problem in the Far East because rice, the
1
major food, has a rather low content of thiamine. This deficiency is partly ameliorated if the whole rice grain is soaked in
water before milling some of the thiamine in the husk then leaches into the rice kernel. The problem is exacerbated if
the rice is polished, because only the outer layer contains significant amounts of thiamine. Beriberi is also occasionally
seen in alcoholics who are severely malnourished and thus thiamine deficient. The disease is characterized by neurologic
and cardiac symptoms. Damage to the peripheral nervous system is expressed as pain in the limbs, weakness of the
musculature, and distorted skin sensation. The heart may be enlarged and the cardiac output inadequate.
BeriberiA vitamin-deficiency disease first described in 1630 by Jacob
Bonitus, a Dutch physician working in Java:
"A certain very troublesome affliction, which attacks men, is called
by the inhabitants Beriberi (which means sheep). I believe those,
whom this same disease attacks, with their knees shaking and the
legs raised up, walk like sheep. It is a kind of paralysis, or rather
Tremor: for it penetrates the motion and sensation of the hands and
feet indeed sometimes of the whole body."
Which biochemical processes might be affected by a deficiency of thiamine? Thiamine pyrophosphate is the prosthetic
group of three important enzymes: pyruvate dehydrogenase, α-ketoglutarate dehydrogenase, and transketolase.
Transketolase functions in the pentose phosphate pathway, which will be discussed in Chapter 20. The common feature
of enzymatic reactions utilizing TPP is the transfer of an activated aldehyde unit. In beriberi, the levels of pyruvate and
α-ketoglutarate in the blood are higher than normal. The increase in the level of pyruvate in the blood is especially
pronounced after the ingestion of glucose. A related finding is that the activities of the pyruvate and α-ketoglutarate
dehydrogenase complexes in vivo are abnormally low. The low transketolase activity of red cells in beriberi is an easily
measured and reliable diagnostic indicator of the disease.
Why does TPP deficiency lead primarily to neurological disorders? The nervous system relies essentially on glucose as
its only fuel. In contrast, most other tissues can use fats as a source of fuel for the citric acid cycle. The product of
aerobic glycolysis, pyruvate, can enter the citric acid cycle only through the pyruvate dehydrogenase complex.
Symptoms similar to those of beriberi arise if an organism is exposed to mercury or arsenite (AsO3 3-). Both elements
have a high affinity for neighboring sulfhydryls, such as those in the reduced dihydrolipoyl groups of the dihydrolipoyl
dehydrogenase component of the pyruvate dehydrogenase complex (Figure 17.20). The binding of mercury or arsenite to
the dihydrolipoyl groups inhibits the complex and leads to central nervous system pathologies. The proverbial phrase
"mad as a hatter" refers to the strange behavior of poisoned hat makers who used mercury nitrate to soften and shape
animal furs. This form of mercury is absorbed through the skin. Similar problems afflicted the early photographers, who
used vaporized mercury to create daguerreotypes.
[The Granger Collection.]
Treatment for these poisons is the administration of sulfhydryl reagents with adjacent sulfhydryl groups to compete with
the dihydrolipoyl residues for binding with the metal ion, which is then excreted in the urine. Indeed, 2,3dimercaptopropanol (see Figure 17.20) was developed after World War I as an antidote to lewisite, an arsenic-based
chemical weapon. This compound was initially called BAL, for British anti-lewisite.
17.3.3. Speculations on the Evolutionary History of the Citric Acid Cycle
How did the citric acid cycle come into being? Although definitive answers are elusive, it is nevertheless
instructive to speculate how this complicated central hub of metabolism developed. We can perhaps begin to
comprehend how evolution might work at the level of biochemical pathways.
The manuscript proposing the citric acid cycle was submitted for
publication to Nature but was rejected. It was subsequently
published in Enzymologia. Dr. Krebs proudly displayed the rejection
letter throughout his career as encouragement for young scientists.
"June 1937
The editor of NATURE presents his compliments to Dr. H. A. Krebs
and regrets that as he has already sufficient letters to fill the
correspondence columns of NATURE for seven or eight weeks, it is
undesirable to accept further letters at the present time on account of
the time delay which must occur in their publication.
If Dr. Krebs does not mind much delay the editor is prepared to keep
the letter until the congestion is relieved in the hope of making use of
it.
He returns it now, in case Dr. Krebs prefers to submit it for early
publication to another periodical."
It is most likely that the citric acid cycle was assembled from preexisting reaction pathways. As noted earlier, many of
the intermediates formed in the citric acid cycle are used in biosynthetic pathways to generate amino acids and
porphyrins. Thus, compounds such as pyruvate, α-ketoglutarate, and oxaloacetate were likely present early in evolution
for biosynthetic purposes. The oxidative decarboxylation of these α-ketoacids is quite favorable thermodynamically. The
elegant modular structures of the pyruvate and α-ketoglutarate dehydrogenase complexes reveal how three reactions
(decarboxylation, oxidation, and thioester formation) can be linked to harness the free energy associated with
decarboxylation to drive the synthesis of both acyl CoA derivatives and NADH. These reactions almost certainly formed
the core of processes that preceded the citric acid cycle evolutionarily. Interestingly, α-ketoglutarate can be directly
converted into oxaloacetate by transamination of the respective amino acids by aspartate aminotransferase, another key
biosynthetic enzyme. Thus, cycles comprising smaller numbers of intermediates could have existed before the present
form evolved to harvest the electrons from pyruvate or other compounds more efficiently.
II. Transducing and Storing Energy
17. The Citric Acid Cycle
17.3. The Citric Acid Cycle Is a Source of Biosynthetic Precursors