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Volume 5, No.1 January 2015 - 日本ブラキスピラ学会
Volume 5, No.1 January 2015 Japanese Society of Brachyspira President Prof. Yoshikazu Adachi Vice President Dr. Sho Ogata 日本ブラキスピラ学会 会長 足 立 吉 數 副会長 緒 方 衝 Editors of Brachyspira Dr. Sho Ogata (Editor-in Chief ) Dr. Kouichi Kadota Dr. Jin Tanahashi Prof. Shinnji Takai Dr. Katsuhiro Mabe Dr. Akihiko Yoshizawa Dr. Takahito Takezawa Prof. Masuo Sueyoshi Prof. Hiromi Nakajima Dr. Tomoyuki Shibahara Prof. Yoshikazu Adachi Assoc. Prof. Kouichi Iwamoto 編集幹事 緒 方 門 田 耕 棚 橋 高 井 伸 間 部 克 吉 澤 明 竹 澤 敬 末 吉 益 中 島 弘 芝 原 友 足 立 吉 岩 本 淳 Secretariat Office Laboratory Animal Health College of Agriculture, Ibaraki University 3-21-1, Ami-machi, Inashiki-gun, Ibaraki, 300-0393, Japan 事務局 〒300-0393 茨城県稲敷郡阿見町中央 3−21−1 国立大学法人茨城大学農学部 動物保健衛生研究室 衝 一 仁 二 裕 彦 人 雄 美 幸 數 一 Brachyspira 2015;5(1): ブラキスピラ 日本ブラキスピラ学会報 第 5 巻 第 1 号 （2015 年 1 月 30 日） 目 次 総 説 豚赤痢および豚腸管スピロヘータ症の実験モデルおよび野外例の 病理学的知見 …………………………………………………………………末吉益雄……… 3 原 著 ホルスタイン種牛における好銀性グラム陽性フィラメント状細菌が 関連した空腸肉芽腫 ………大泉卓也、矢彦沢小百合、稲垣華絵、播谷 亮、芝原友幸……… 12 教育講座 Brachyspira と豚赤痢 ……………………………………………………芝原友幸、稲垣華絵……… 21 第 6 回日本ブラキスピラ学会総会及び学術集会終了報告 …………………………………………………………………岩本淳一……… 38 投稿規定……………………………………………………………………………… 44 会務報告……………………………………………………………………………… 53 Brachyspira 2015;5(1): Brachyspira Journal of the Japanese Society of Brachyspira Volume 5, Number 1, January 30, 2015 Contents Review Sueyoshi M.: Pathological study on an experiment model and field cases of swine dysentery and porcine intestinal spirochaetosis… …………………… 3 Regular paper Oizumi T., Yahikozawa S., Inagaki H., Haritani M., Shibahara T.: Jejunal granuloma associated with argyrophilic, gram-positive filamentous bacteria in a Holstein cow… …………………………………………………… 12 Educational lecture Shibahara T., Inagaki H.: Brachyspira and swine dysentery… ………………… 21 Report on the 6th meeting of the Japanese Society of Brachyspira ……………………………………………………………… Iwamoto J.……… 38 Instructions to Authors… …………………………………………………………… 44 Newsletter from the office… ………………………………………………………… 53 Brachyspira 2015:5(1):4 Sueyoshi M.: ⥲ㄝ㸸 ㇜㉥⑩䛚䜘䜃㇜⭠⟶䝇䝢䝻䝦䞊䝍䛾ᐇ㦂䝰䝕䝹䛚䜘䜃㔝እ䛾⌮Ꮫⓗ▱ぢ ᮎྜྷ┈㞝 ᐑᓮᏛ⏘ᴗື≀㜵䝸䝃䞊䝏䝉䞁䝍䞊㜵ᡓ␎㒊㛛 䜻䞊䝽䞊䝗䠖㇜㉥⑩䠗㇜⭠⟶䝇䝢䝻䝦䞊䝍䠗ᐇ㦂䝰䝕䝹 1. ㅖゝ ື≀䛾䝇䝢䝻䝦䞊䝍ᛶ⭠⅖䛸䛧䛶䚸㇜㉥⑩䛚䜘䜃㇜⭠⟶䝇䝢䝻䝦䞊䝍䛜䛒 䜛 1, 2) 䚹 ㇜㉥⑩䛿⢓⾑ୗ⑩౽䜢ᚩ䛸䛩䜛ᛴᛶ䜎䛯䛿៏ᛶ䛾㇜䛾ఏᰁᛶ⭠ᝈ 䛷䛒䜛 2)䚹ཎᅉ⳦䛿 Brachyspira hyodysenteriae 䛷䛒䜚䚸㛗䛥 6䡚10 ȝm䚸ᖜ 0.3 䡚0.4 ȝm 䛾ᆺ䛾䜙䛫䜣⳦䛷䛒䜛䚹㉥⑩Ⓨ䛻䛿䚸ᮏ⳦䛾ឤᰁ䛻ຍ䛘䛶䚸 Fusobacterium ᒓ䚸Bacteroides ᒓ䛒䜛䛔䛿 Clostridium ᒓ䛺䛹䛾⭠ෆ⣽⳦ྀ䛜 㛵䛧䛶䛔䜛䛸䛥䜜䚸ᮏ⳦༢⊂䛾㇜䜈䛾⤒ཱྀᢞ䛷䛾⌧ᐇ㦂䛿䜋䛸䜣䛹ኻᩋ 䛧䛶䛔䜛䚹 ㇜⭠⟶䝇䝢䝻䝦䞊䝍䛾ཎᅉ⳦䛿 B. pilosicoli 䛷䛒䜚䚸⤒ཱྀឤᰁ䛩䜛 1) 䚹B. pilosicoli 䛿䝠䝖䚸㟋㛗㢮䚸䜲䝚䚸㱎ṑ㢮䚸㫽㢮䛛䜙䜒ศ㞳䛥䜜䚸䝈䞊䝜䞊䝅䝇䛸䛧 䛶䜒㔜せ䛷䛒䜛䚹≧䛸䛧䛶䚸ᚤ㔞䛾⢓ᾮ䛸⾑ᾮ䜢ྵ䜐⥳Ⰽ䛛䜙〓Ⰽ䜢ᖏ䜃䛯 ㌾౽䛒䜛䛔䛿Ỉᵝᛶୗ⑩䛜ㄆ䜑䜙䜜䜛䚹Ṛஸ䛿⛥䛷䚸Ⓨ⫱㐜ᘏ䚸㣫ᩱຠ⋡䛾ప ୗ䛜䜏䜙䜜䜛䚹 ᮏ✏䛷䛿䚸䛣䜜䜙䛾ື≀䛾䝇䝢䝻䝦䞊䝍䛾ᐇ㦂䝰䝕䝹䛚䜘䜃⮬↛ឤᰁ 䛻䛴䛔䛶⌮Ꮫⓗᡤぢ䜢ᴫㄝ䛩䜛䚹 2. ㇜㉥⑩ B. hyodysenteriae 䛾ึ⏕㞮䜈䛾ឤᰁᐇ㦂䛷䛿䚸B. hyodysenteriae 䛾ᐃ╔䛜 㧗⋡䛻☜ㄆ䛥䜜䛯(⾲ 1)䚹B. hyodysenteriae 䛿┣⭠䛻ᐃ╔䛧䚸┣⭠⢓⭷ୖ⓶⣽ ⬊⾲㠃䛻╔䛧䛯ᚋ䚸ᚤ⤧ẟ䜢◚ቯ䛧䚸⢓⭷ୖ⓶⣽⬊ᇶᗏ⭷ୗ䛻ධ䛧䚸⢓ ⭷ᅛ᭷ᒙ䜎䛷㐩䛧䚸⢓⭷䛾ቯṚኚ䜢ᙧᡂ䛧䛯(ᅗ 1-3)䚹䛭䛾ᚋ䚸㝜❐䛾ᖿ⣽ Brachyspira 2015:5(1):52015:5(1):5 Brachyspira Brachyspira 2015;5(1): Brachyspira 2015:5(1):5 ⬊䚸ፉ⣽⬊䛾ศ䛷䚸㝜❐ୖ⓶⣽⬊䛾⏕䛜άᛶ䛧䚸ୖ⓶⣽⬊䛾㐣ᙧᡂᮇ 䛸䛺䜚䚸ᮼ⣽⬊䛿ஈ䛧䛟䚸⢓⭷䛾⫧ཌ䛜ほᐹ䛥䜜䛯䚹 ⬊䚸ፉ⣽⬊䛾ศ䛷䚸㝜❐ୖ⓶⣽⬊䛾⏕䛜άᛶ䛧䚸ୖ⓶⣽⬊䛾㐣ᙧᡂᮇ ⬊䚸ፉ⣽⬊䛾ศ䛷䚸㝜❐ୖ⓶⣽⬊䛾⏕䛜άᛶ䛧䚸ୖ⓶⣽⬊䛾㐣ᙧᡂᮇ 䛸䛺䜚䚸ᮼ⣽⬊䛿ஈ䛧䛟䚸⢓⭷䛾⫧ཌ䛜ほᐹ䛥䜜䛯䚹 䛸䛺䜚䚸ᮼ⣽⬊䛿ஈ䛧䛟䚸⢓⭷䛾⫧ཌ䛜ほᐹ䛥䜜䛯䚹 ⾲ 1. B. hyodysenteriae ឤᰁ 21 ᪥ᚋࡢᗂ㞮┣⭠ࡽࡢศ㞳⳦ᩘ┣⭠ᡤぢ ⾲ 1. B. hyodysenteriae ឤᰁ 21 ᪥ᚋࡢᗂ㞮┣⭠ࡽࡢศ㞳⳦ᩘ┣⭠ᡤぢ ┣⭠ኚ ෆᐜ≀ ⾲ 1. B. hyodysenteriae ឤᰁ 21 ᪥ᚋࡢᗂ㞮┣⭠ࡽࡢศ㞳⳦ᩘ┣⭠ᡤぢ 㞮ᩘ ⳦ᩘ 㞮ᩘ ⳦ᩘ 21 ឤᰁ㞮 㞮ᩘ ឤᰁ㞮 㠀ឤᰁ㞮 7.52±0.17a ⳦ᩘ 21 10 a 7.52±0.17 0 㠀ឤᰁ㞮 10 log10CFU/g(ᖹᆒ್±SE) 0 a ឤᰁ㞮 a ⴎ⦰ 18/21 10 ┣⭠ኚ ⢓⭷⫧ཌ ⓑⰍ⢓ᾮ 17/21 ⓑⰍ⢓ᾮ 15/21 18/21 0/10 17/21 0/10 15/21 0/10 0/10 0/10 0/10 ⴎ⦰ 21 7.52±0.17a log10CFU/g(ᖹᆒ್±SE) 㠀ឤᰁ㞮 a ┣⭠ኚ ⴎ⦰ ⢓⭷⫧ཌ 0 ⢓⭷⫧ཌ ෆᐜ≀ ࢳ࣮ࢬᵝ ⾑ᵝෆᐜ ෆᐜ≀ ࢳ࣮ࢬᵝ ≀ ⾑ᵝෆᐜ ෆᐜ≀ 7/21 ≀ 1/21 ෆᐜ≀ 7/21 0/10 ࢳ࣮ࢬᵝ 1/21 0/10 ⾑ᵝෆᐜ 0/10 ෆᐜ≀ 0/10 ≀ ⓑⰍ⢓ᾮ 18/21 17/21 15/21 7/21 1/21 0/10 0/10 0/10 0/10 0/10 ⾲ 2. B. pilosicoli ឤᰁ 1㹼3 㐌ᚋࡢᗂ㞮┣⭠ࡽࡢศ㞳⳦ᩘ┣⭠ᡤぢ ⾲ 2. B. pilosicoli ឤᰁ 1㹼3 㐌ᚋࡢᗂ㞮┣⭠ࡽࡢศ㞳⳦ᩘ┣⭠ᡤぢ B. pilosicoli ศ㞳 ┣⭠ኚ log10CFU/g(ᖹᆒ್±SE) ឤᰁᚋ⤒㐣㐌 B. pilosicoli ศ㞳 ⳦ศ㞳㞮ᩘ/ឤᰁ㞮 ศ㞳⳦ᩘ a ┣⭠ኚ ኚⓎ⌧/ឤᰁ㞮 ⳦ศ㞳㞮ᩘ/ឤᰁ㞮 10/10 ศ㞳⳦ᩘ a 9.16s0.11 ኚⓎ⌧/ឤᰁ㞮 10/10 1wk 2wks 10/10 9.16s0.11 7.97s0.91 10/10 8/10 2wks 3wks 10/10 7.97s0.91 6.92s1.30 8/10 5/10 ឤᰁᚋ⤒㐣㐌 1wk ⾲ 2. B. pilosicoli ឤᰁ 1㹼3 㐌ᚋࡢᗂ㞮┣⭠ࡽࡢศ㞳⳦ᩘ┣⭠ᡤぢ a 6.92s1.30 3wks 10/10 B. ᢞ)15 log10CFU/g(ᖹᆒ್±SE)ࠊᑐ↷㞮(TSB ⩚ࡽࡢ⳦ศ㞳ࡣ 0/5 ⩚/㐌 pilosicoli ศ㞳 ឤᰁᚋ⤒㐣㐌 a 5/10 log10CFU/g(ᖹᆒ್±SE)ࠊᑐ↷㞮(TSB ᢞ)15 ⩚ࡽࡢ⳦ศ㞳ࡣ 0/5 ⩚/㐌 ⳦ศ㞳㞮ᩘ/ឤᰁ㞮 ศ㞳⳦ᩘ a ኚⓎ⌧/ឤᰁ㞮 1wk 10/10 9.16s0.11 10/10 2wks 10/10 7.97s0.91 8/10 10/10 6.92s1.30 5/10 3wks a ┣⭠ኚ ᅗ 1. ┣⭠⢓⭷ࠋච⤌⧊ᏛᰁⰍࠋ ᅗ 1. ┣⭠⢓⭷ࠋච⤌⧊ᏛᰁⰍࠋ ᢞ)15 ⩚ࡽࡢ⳦ศ㞳ࡣ 0/5 ⩚/㐌 log10CFU/g(ᖹᆒ್±SE)ࠊᑐ↷㞮(TSB 図 1. 盲腸粘膜。免疫組織化学染色。 ᅗ 1. ┣⭠⢓⭷ࠋච⤌⧊ᏛᰁⰍࠋ Brachyspira 2015:5(1):6 Sueyoshi M.: 図 2. 盲腸粘膜。透過型電子顕微鏡写真。 ᅗ 2. ┣⭠⢓⭷ࠋ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾┿ࠋ 図 3. 盲腸粘膜。透過型電子顕微鏡写真。 ᅗ 3. ┣⭠⢓⭷ࠋ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾┿ࠋ ᅗ 4. ㇜㉥⑩⨯ᝈ㇜ࡢ⣅౽ࡢ┤᥋ሬᶆᮏࠋᬯど㔝㢧ᚤ㙾┿ࠋ 図 4. 豚赤痢罹患豚の糞便の直接塗抹標本。暗視野顕微鏡写真。 ㇜㉥⑩⨯ᝈ㇜䛾⣅౽䛾┤᥋ሬᶆᮏ䜢ᬯど㔝㙾᳨䛩䜛䛸⃭䛧䛟㐠ື䛩䜛 ᆺ䜙䛫䜣⳦䛜ほᐹ䛥䜜䜛(ᅗ 4)䚹䛭䛾⭠⟶䜢ほᐹ䛩䜛䛸䚸ኚ䛿䚸┣⭠䚸⤖⭠䛚 䜘䜃┤⭠䛾⭠䛻㝈ᒁ䛧䛶ㄆ䜑䜙䜜䚸⭠ቨ䛾Ỉ⭘ᛶ⫧ཌ䚸⢓⭷䛾ᬯ㉥Ⰽ䛚 䜘䜃ฟ⾑䛜ㄆ䜑䜙䜜䜛䚹⾲㠃䛿䚸⢓⛻䛺⢓ᾮ䜔⾑ᾮ䛾ΰᅾ䛧䛯ⁱฟᾮ䛷⿕䜟 䜜䜛(ᅗ 5)䚹ഇ⭷䜢ᙧᡂ䛩䜛䛣䛸䜒䛒䜛䚹⤌⧊ᡤぢ䛷䛿䚸⢓⭷ୖ⓶⣽⬊䛾ኚᛶ䚸 ቯṚ䚸㞳䞉⬺ⴠ䛜ⴭ᫂䛷䚸ฟ⾑䚸⣽⬊㢘ᗫ≀䛚䜘䜃⥺⥔⣲䛾ᯒฟ䛜ㄆ䜑䜙䜜䚸 ⢓⭷ᅛ᭷ᒙ䛻䛿ዲ୰⌫䛾ᾐ₶䛜䜏䜙䜜䜛䚹㝜❐䛷䛿ୖ⓶⣽⬊䛾㐣ᙧᡂ䚸㝜❐ ⭍䛾ᣑᙇ䚸⢓ᾮ䛾‶䛜ㄆ䜑䜙䜜䜛䚹ᮏ⳦䛜⢓⭷⾲㠃䚸㝜❐⭍ෆ䛚䜘䜃ᮼ⣽ ⬊➼䛾ୖ⓶⣽⬊㉁ෆ䛻ㄆ䜑䜙䜜䜛(ᅗ 6)䚹៏ᛶ䛾ሙྜ䚸⢓⭷䛿⫧ཌ䛧䚸㝜❐䛾 ୖ⓶⣽⬊䛾ⴭ᫂䛺㐣ᙧᡂ䛜ㄆ䜑䜙䜜䜛䚹䛣䜜䜙䛾䛣䛸䛛䜙䚸⏕⏘⌧ሙ䛷Ⓨ⏕䛧䛶 䛔䜛㇜㉥⑩ኚ䛿ᗂ㞮䝰䝕䝹┣⭠ኚ䛸ᇶᮏⓗ䛻ྠ䛨䛷䛒䜛䛜䚸䜘䜚㔜ᗘ䛷䛒 Brachyspira 2015;5(1): Brachyspira 2015:5(1):7 䜚䚸ᮏ⳦௨እ䛾せᅉឤస䛜⪃䛘䜙䜜䜛䚹 ᅗ 6. ㇜㉥⑩⨯ᝈ㇜ࡢ⤖⭠⢓⭷ࠋWS ᰁⰍࠋ ᅗ 図 5. 豚赤痢罹患豚の結腸粘膜。 5. ㇜㉥⑩⨯ᝈ㇜ࡢ⤖⭠⢓⭷ࠋ 図 6. 豚赤痢罹患豚の結腸粘膜。WS 染色。 3.㻌 ㇜⭠⟶䝇䝢䝻䝦䞊䝍 B. pilosicoli 䛾ึ⏕㞮䜈䛾ឤᰁᐇ㦂䛷䚸B. pilosicoli 䛿┣⭠䛻ᐃ╔䛧(⾲ 2)䚸䝇 䝢䝻䝦䞊䝍䛾ᚤᑠ㞟ⴠ䛜⢓⭷⾲㠃䛻ㄆ䜑䜙䜜䛯䚹᥋╔䛧䛯⳦㞟ⴠ䛿ᣑ䛧䚸㞄 ᥋䛸⧅䛜䜚䚸⢓⭷⾲㠃䜢そ䛖⌧㇟䛜⤒ⓗほᐹ䛷ㄆ䜑䜙䜜䛯(ᅗ 7)䚹䝇䝢䝻䝦䞊 䝍䛾 end-on ᥋╔䛜⭠⢓⭷྾ୖ⓶⣽⬊䛾⣽⬊⭷䛻ᩚิ䛧䛶ㄆ䜑䜙䜜䚸䛭䛾㒊 䛷䛿䚸⣽⬊䛾ᚤ⤧ẟ䛾ኚᛶ䚸core filament 䛾㓄ิᩚ䛒䜛䛔䛿᰾ୖ㒊ᇶ㉁ 䛾Ỉ⭘ᛶኚᛶ䚸✵⬊ኚᛶ䚸⣽⬊⾲㠃䛾ங㢌≧㝯㉳䛒䜛䛔䛿㞳䛜ㄆ䜑䜙䜜䛯 (ᅗ 8)䚹 Sueyoshi M.: 図 7. 盲腸粘膜。走査型電子顕微鏡写真。 Brachyspira 2015:5(1):9 Brachyspira 2015;5(1): ឤᰁ1㐌㛫ᚋ ឤᰁ2㐌㛫ᚋ ឤᰁ2㐌㛫ᚋ ឤᰁ3㐌㛫ᚋ ᅗ 図 8. 盲腸粘膜。透過型電子顕微鏡写真。 8. ┣⭠⢓⭷ࠋ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾┿ࠋ ᪥ᮏᅜෆ䛻䛚䛡䜛㇜⭠⟶䝇䝢䝻䝦䞊䝍䛾Ⓨ⏕≧ἣ䛿ᮍ䛰᫂䜙䛛䛷䛿䛺䛔䚹 㛗ᓮ┴䛾⣙ 1,400 㢌㣫㣴䛾୍㈏⤒Ⴀ㣴㇜ሙ䛻䛚䛔䛶䚸㞳ஙᚋ⣙ 1 䞄᭶䛾㇜⩌ 䛻ୗ⑩䛜Ⓨ⏕䛧䚸䛭䛾⨯ᝈ㇜䛻䛴䛔䛶ᛶ㚷ᐃ䜢ᐇ䛧䛯䛸䛣䜝䚸PRDC 䜢క 䛳 䛯 」 ྜ ឤ ᰁ ᛶ ⭠ ⅖ 䛜 ㄆ 䜑 䜙 䜜 䛯 䚹 ⭠ ⅖ 䛾 ཎ ᅉ 䛸 䛧 䛶 䚸 Salmonella Typhimurium 䚸 Escherichia coli 䚸 Cryptosporidium 䚸 Blantidium 䛚 䜘 䜃 B. pilosicoli 䛜᳨ฟ䛥䜜䛯䚹⭠⢓⭷䛷䛿䚸B. pilosicoli ឤᰁ䛾ᗂ㞮䛾⭠ኚ䛸 ᇶᮏⓗ䛻ྠᵝ䚸⦆䜔䛛䛺䝇䝢䝻䝦䞊䝍䛜㞟ሢ䜢ᙧᡂ䛧䛶⢓⭷⾲㠃䜢⿕そ䛧(ᅗ 9)䚸⳦䛿䚸∦ഃඛ➃㒊䜢⢓⭷ୖ⓶⣽⬊⾲㠃䛻ᐦ䛻᥋╔䛧䚸⢓⭷ୖ⓶⣽⬊䛾ᚤ ⤧ẟ䛿ᾘኻ䛧䛶䛔䛯(ᅗ 10-11)䚹䜎䛯䚸୍㒊䛾⳦䛿ୖ⓶⣽⬊㛫䛻ධ䛧䛶䛔䛯䚹 ᮏ䛿䚸✀䚻䛾ཎయ䛻䜘䜛」ྜឤᰁ䛷䛒䛳䛯䚹 Brachyspira 2015:5(1):10 Sueyoshi M.: ᅗ 9. ⭠⢓⭷ࠋ㉮ᰝᆺ㟁Ꮚ㢧ᚤ㙾┿ࠋ 図 9. 大腸粘膜。走査型電子顕微鏡写真。 ᅗ 10. ⭠⢓⭷ࠋ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾┿ࠋ 図 10. 大腸粘膜。透過型電子顕微鏡写真。 4. せ⣙ B. hyodysenteriae 䛚 䜘 䜃 B. pilosicoli 䛾ึ⏕㞮ឤᰁ䝰䝕䝹㛫 䛾ẚ㍑䛷䛿䚸⢓⭷ୖ⓶⣽⬊䜈䛾 ╔ᵝᘧ䛚䜘䜃ධᵝᘧ䛜␗䛺䜚䚸B. hyodysenteriae 䛾᪉䛜ᙉ䛔ධᛶ 䜢♧䛧䛯䚹ᮏᐟ䛷䛒䜛㇜䛾ሙྜ 䛻䜒䛭䜜䜙䛾ኚⓎ⌧ᶵᗎ䛿᫂䜙 䛛㇜㉥⑩䛸㇜⭠⟶䝇䝢䝻䝦䞊䝍 䛾⭠ኚ䜢ẚ㍑䛩䜛䛸␗䛺䛳䛶 䛔䛯䚹㇜㉥⑩䛷䛿䚸⭠⢓⭷䛾⾲ ᒙቯṚ䚸ฟ⾑䛜ほᐹ䛥䜜䜛䛜䚸㇜ ᅗ11. ⭠⢓⭷ࠋච㟁Ꮚ㢧ᚤ㙾┿ࠋ୍ḟᢠయ:: B. 図 B. 11. 大腸粘膜。免疫電子顕微鏡写真。一次抗体 pilosicoli ウサギ血清 pilosicoli ࢘ࢧࢠ⾑Ύ ⭠⟶䝇䝢䝻䝦䞊䝍䛾ሙྜ䚸⭠⢓ ⭷യᐖ䛿㍍ᗘ䛷䛒䜚䚸ᗈ⠊ቯṚ䛿 䜏䜙䜜䛺䛛䛳䛯䚹 㐃⤡㈐௵⪅䠖ᮎྜྷ┈㞝㻌 䛈889-2192 ᐑᓮᕷᏛᅬᮌⰼྎす 1-1 ᐑᓮᏛ⏘ᴗ ື≀㜵䝸䝃䞊䝏䝉䞁䝍䞊㜵ᡓ␎㒊㛛; tel&fax: 0985587282; email address: [email protected] (ㄽᩥཷ䠖䠎䠌䠍䠐ᖺ䠍䠎᭶䠍᪥䠗ཷ⌮㻌 䠎䠌䠍䠐ᖺ䠍䠎᭶䠏᪥) 䠆 Brachyspira 2015:5(1):11 Brachyspira 2015;5(1): 10 5. ᘬ⏝ᩥ⊩ 1) Hampson DJ, et al. (2006) Porcine colonic spirochaetosis/intestinal spirochaetosis. In: Straw BE, et al., eds. Diseases of Swine. 9th ed. p755-767, Blackwell Publishing, Ames, Iowa. 2) Hampson DJ, et al. (2006) Swine dysentery. In: Straw BE, et al., eds. Diseases of Swine. 9th ed. p785-805 Blackwell Publishing, Ames, Iowa. Review Pathological study on an experiment model and field cases of swine dysentery and porcine intestinal spirochaetosis Masuo SUEYOSHI The Center for Animal Disease Control, University of Miyazaki Abstract Swine dysentery and porcine intestinal spirochaetosis are two major diseases causing spirochetal enteritis in pigs. Swine dysentery is a contagious large intestinal disease of the pigs, that affected pigs exhibit hemorrhagic diarrhea. The active pathogen is Brachyspira hyodysenteriae. Diarrhea containing mucous and the blood is confirmed as porcine intestinal spirochaetosis. The pathogen is B. pilosicoli. B. pilosicoli can be isolated from a human, a primate, a dog, rodents and birds, and it is also important as zoonosis. We succeeded in performing a reproduction of the characteristic mucosal lesion of the spirochetal enteritis by the infection of a young chicken with B. hyodysenteriae and B. pilosicoli. In this report, I describe an outline of the pathological views of an experimental model and a natural infection of an animal with spirochaetosis. Sueyoshi M.: 11 Brachyspira 2015:5(1):12 Key words: swine dysentery; pig intestinal spirochaetosis; experimental model Corresponding author: Masuo Sueyoshi, The center for Animal Disease control, University of Miyazaki, 1-1 Nishi, Gakuen kihanadai Miyazaki, 889-2192, Japan; tel & fax: 0985587282; email address: [email protected] (This paper was received on December 1, 2014, and accepted on December 3, 2014.) Brachyspira 2015:5(1):13 Brachyspira 2015;5(1): 12 ཎⴭㄽᩥ㸸 ࣍ࣝࢫࢱࣥ✀∵࠾ࡅࡿዲ㖟ᛶࢢ࣒ࣛ㝧ᛶࣇ࣓ࣛࣥࢺ≧⣽ ⳦ࡀ㛵㐃ࡋࡓ✵⭠⫗ⱆ⭘ Ἠ༟ஓ㸯ͤࠊ▮ᙪἑᑠⓒྜ㸰ࠊ✄ᇉ⳹⤮㸱ࠊ㇂ு㸱ࠊⰪཎᖾ㸱 㸯 㛗㔝┴ᯇᮏᐙ␆ಖ⾨⏕ᡤࠊ㸰㛗㔝┴㣤⏣ᐙ␆ಖ⾨⏕ᡤࠊ㸱(⊂) ㎰ ᴗ࣭㣗ရ⏘ᴗᢏ⾡⥲ྜ◊✲ᶵᵓ ື≀⾨⏕◊✲ᡤ ࣮࣮࢟࣡ࢻ: ࢢ࣒ࣛ㝧ᛶࣇ࣓ࣛࣥࢺ≧⣽⳦㸹⫗ⱆ⭘㸹✵⭠ せ⣙ 30 ࣨ᭶㱋ࡢ࣍ࣝࢫࢱࣥ✀∵ࡢ㛢ሰࡋࡓ✵⭠ࡀእ⛉ⓗฟࡉࢀࡓࠋ⫗ ║ⓗࠊ㛢ሰ㒊ࡢ✵⭠ቨ⭘⒗ࡀ」ᩘㄆࡵࡽࢀࡓࠋ⌮⤌⧊Ꮫⓗࠊ⭘⒗ ࡣ㢮ୖ⓶⣽⬊ᑡᩘࡢከ᰾ᕧ⣽⬊ࡼࡿከⓎᛶ⫗ⱆ⭘࡛ᵓᡂࡉࢀ࡚࠸ࡓࠋ 」ᩘࡢዲ㖟ᛶࠊࢢ࣒ࣛ㝧ᛶࠊ㐣ࣚ࢘⣲㓟ࢩࢵࣇᛂ㝜ᛶࡢࣇ࣓ࣛࣥࢺ≧ ⣽⳦ࡀ㢮ୖ⓶⣽⬊ࡸከ᰾ᕧ⣽⬊ࡢ⣽⬊㉁ෆ᳨ฟࡉࢀࡓࠋᢠ㓟⳦ࡣ᳨ฟࡉ ࢀ࡞ࡗࡓࠋࡇࡢዲ㖟ᛶࢢ࣒ࣛ㝧ᛶࣇ࣓ࣛࣥࢺ≧⣽⳦ࡀᙜヱ∵ࡢ✵⭠⫗ ⱆ⭘ࢆᘬࡁ㉳ࡇࡋࠊ⭠㛢ሰࡀㄏⓎࡉࢀࡓ᥎ᐹࡉࢀࡓࠋࡇࡢࡼ࠺࡞ࣇࣛ ࣓ࣥࢺ≧⣽⳦ࡼࡿ⛥࡞ែࢆデ᩿ࡍࡿ࠶ࡓࡾࠊBrachyspira ᒓ⣽⳦ࢆ ྵࡵࡓ㚷ูデ᩿ࡀᚲせ࡛࠶ࡿࠋ ࠙⥴ゝࠚ ∵ࡢᾘ⟶ኚࢆច㉳ࡍࡿ⣽⳦࡛ࡣࠊᮍࡔヲ⣽ࡀ᫂ࡽ࡛࡞࠸ࡶࡢࡀᏑ ᅾࡍࡿࠋ1997 ᖺ 28 ᪥㱋ࡢᏊ∵࡛ࢢ࣒ࣛ㝧ᛶศ⠇ᛶࣇ࣓ࣛࣥࢺ≧⣽⳦ ࡀሗ࿌ࡉࢀ࡚࠾ࡾࠊࡇࡢ⣽⳦ࡣ⭠⤧ẟ╔ࡋ㍍ᚤ࡞ኚᙧᡂ㛵㐃ࡋ࡚ ࠸ࡿࡀࠊ⌮Ꮫⓗព⩏ࡣ࡛᫂࠶ࡿ㹙8㹛ࠋྠᵝࡢ⣽⳦ࡣ㤿㹙4㹛ࠊ㇜㹙7㹛ࠊ ≟㹙2㹛ࠊ࣐࢘ࢫࠊࣛࢵࢺ㹙9㹛ཬࡧ㭜㹙6㹛࡛ㄆࡵࡽࢀ࡚࠸ࡿࠋࡇࢀࡽࡢ⣽ T, et al.: 13 BrachyspiraOizumi 2015:5(1):14 ⳦ࡣ⏕యእ࡛ᇵ㣴ࡍࡿࡇࡀ࡛ࡁࡎࠊศ㢮ࡸཎᛶࡣゎࡗ࡚࠸࡞࠸㹙1㸪9㹛ࠋ ᡃࡀᅜ࡛ࡣࠊ✵⭠⫗ⱆ⭘ᛶ⅖ࢆច㉳ࡍࡿࣇ࣓ࣛࣥࢺ≧⣽⳦ࡀሗ࿌ࡉ ࢀ࡚࠸ࡿ㹙3㸪5㹛ࡀࠊࡇࢀࡽ 2 ࡣ࠸ࡎࢀࡶᗈᓥ┴࡛㣫⫱ࡉࢀ࡚࠸ࡓ࣍ ࣝࢫࢱࣥ✀ᡂ∵࡛࠶ࡾࠊࡇࡢࡼ࠺࡞ࡣୡ⏺ⓗࡶሗ࿌ࡀ࡞࠸ࠋ 2011 ᖺࠊ8 ṓ㱋ࡢ࣍ࣝࢫࢱࣥ✀∵࡛ዲ㖟ᛶࢢ࣒ࣛ㝧ᛶศ⠇ᛶࣇ࣓ࣛ ࣥࢺ≧⣽⳦ࡀከᕢᛶ⫗ⱆ⭘ᛶ✵⭠⅖ࢆᘬࡁ㉳ࡇࡋࠊ⭠㔜✚ࡀㄏⓎࡉࢀࡓ ሗ࿌ࡉࢀ࡚࠸ࡿ㹙3㹛ࠋࡲࡓࠊ2010 ᖺࡣ 35 ࣨ᭶㱋ࡢ࣍ࣝࢫࢱࣥ✀∵ ࡛⭠㔜✚ࢆㄏⓎࡋࡓ✵⭠⫗ⱆ⭘ࡽዲ㖟ᛶࢢ࣒ࣛ㝜ᛶ㠀ศ⠇ᛶࣇ࣓ࣛ ࣥࢺ≧⣽⳦ࡀ᳨ฟࡉࢀ࡚࠸ࡿ㹙5㹛ࠋච⤌⧊Ꮫⓗࠊࡇࡢࣇ࣓ࣛࣥࢺ ≧ ⣽ ⳦ ࡢ ⳦ య ࡢ ୍ 㒊 ࡣ Treponema pallidum ཬ ࡧ Brachyspira hyodysenteriae ᑐࡍࡿ࢘ࢧࢠ࣏ࣜࢡ࣮ࣟࢼࣝᢠయᕪᛂࡍࡿࡇ ࡀ☜ㄆࡉࢀ࡚࠸ࡿ㹙5㹛ࠋ࠸ࡎࢀࡢ⣽⳦ࡶ⳦✀ࡣྠᐃࡉࢀ࡚࠸࡞࠸ࡶࡢࡢࠊ 16S rDNA 㑇ఏᏊሷᇶ㓄ิゎᯒࡢẚ㍑࡛ᮍศ㢮ࡢ✀࡛࠶ࡿࡇࡀ᫂ࡽ ࡞ࡗ࡚࠸ࡿ㹙3㸪5㹛ࠋ ᅇࠊᡃࠎࡣ㛗㔝┴࡛㣫⫱ࡉࢀ࡚࠸ࡓ࣍ࣝࢫࢱࣥ✀ᡂ∵ࡢ✵⭠㛢ሰ㒊 ㄆࡵࡽࢀࡓ⭘⒗ࡘ࠸࡚⌮⤌⧊Ꮫⓗ᳨ᰝࢆᐇࡋ㢮ఝࡢ⣽⳦ࢆ᳨ฟ ࡋࡓࡢ࡛ࠊࡑࡢヲ⣽ࢆሗ࿌ࡍࡿࠋࡲࡓࠊBrachyspira ᒓ⣽⳦ࡢ┦㐪ࠊ㚷 ูࡘ࠸࡚グ㍕ࡍࡿࠋ ࠙ᮦᩱཬࡧ᪉ἲࠚ 㸯 ᮦᩱ 30 ࣨ᭶㱋ࡢ㞤ࡢ࣍ࣝࢫࢱࣥ✀∵ࡀࡢ∵ 60 㢌ࡶ㛗㔝┴ࡢ㎰ሙ ࡛㣫㣴ࡉࢀ࡚࠸ࡓࠋ㎰ሙࡢࡢ∵⮫ᗋⓗ࡞␗ᖖᡤぢࡣㄆࡵࡽࢀ࡞ࡗࡓ ࡀࠊ2012 ᖺ 10 ᭶ᙜヱ∵ࡣ㌾౽ࢆ࿊ࡋࠊ⩣᪥౽Ṇࠊ㣗ḧపୗࢆ࿊ࡋࠊ ⭠㛢ሰࢆࢃࢀእ⛉ⓗ✵⭠ࡀฟࡉࢀࡓࠋฟࡉࢀࡓ✵⭠ࡣ⏕⌮㣗ሷỈ ୰ධࢀࡽࢀࠊ㛗㔝┴ᯇᮏᐙ␆ಖ⾨⏕ᡤᦙධࡉࢀࡓࠋ 㸰 ⌮ゎ๗Ꮫⓗ᳨ᰝ ⏕⌮㣗ሷỈᾐₕࡉࢀࡓ✵⭠ࢆษࡾ㛤ࡁࠊ₢⭷㠃ཬࡧ⢓⭷㠃ࡘ࠸࡚⫗ ║ⓗほᐹࡋࡓࠋほᐹᚋࠊ10㸣୰ᛶ⦆⾪࣐࣍ࣝࣜࣥᾮ୰ධࢀᾐ㏱ᅛᐃࡋ ࡓࠋᅛᐃᚋࠊ✵⭠ࡳࡽࢀࡓ⭘⒗ࡢ㠃ࢆほᐹࡋࡓࠋ Brachyspira 2015;5(1): 14 Brachyspira 2015:5(1):15 㸱 ⌮⤌⧊Ꮫⓗ᳨ᰝ ᅛᐃࡋࡓ✵⭠ࢆษࡾฟࡋࠊᐃἲᚑ࠸ࣃࣛࣇࣥษ∦ࢆసᡂࡋࡓࠋษ∦ ࡣ࣐࣊ࢺ࢟ࢩ࣭࢚ࣜࣥ࢜ࢪࣥᰁⰍ㸦HE ᰁⰍ㸧 ࠊࢢ࣒ࣛᰁⰍࠊ㐣ࣚ࢘⣲㓟ࢩ ࢵࣇᛂ㸦PAS ᛂ㸧ࠊࢳ࣮࣭ࣝࢿࣝࢮࣥᰁⰍཬࡧ࣮࣡ࢳ࣭ࣥࢫࢱ࣮࣮ࣜ ᰁⰍ㸦WS ᰁⰍ㸧ࢆᐇࡋࡓࠋ 㸲 ච⤌⧊Ꮫⓗ᳨ᰝ ✵⭠ࡢࣃࣛࣇࣥษ∦ࢆ⏝࠸ࠊච⤌⧊Ꮫⓗ᳨ᰝࢆᐇࡋࡓࠋ࢘ࢧࢠ ᢠ T. pallidum ࣏ࣜࢡ࣮ࣟࢼࣝᢠయ㸦1,024 ಸᕼ㔘ࠊViroStatࠊ⡿ᅜ㸧ࠊ࢘ ࢧࢠᢠ B. hyodysenteriae serotype-A ࣏ࣜࢡ࣮ࣟࢼࣝᢠయ㸦4,096 ಸᕼ㔘ࠊ ື≀⾨⏕◊✲ᡤ㸧ཬࡧ࢘ࢧࢠᢠ B. pilosicoli ࣏ࣜࢡ࣮ࣟࢼࣝᢠయ㸦4,096 ಸᕼ㔘ࠊື≀⾨⏕◊✲ᡤ㸧ࢆ୍ḟᢠయࡋ࡚⏝࠸ࡓࠋ௨ᚋࡢ᧯సࡣᕷ㈍࢟ ࢵࢺ㸦ࣄࢫࢺࣇࣥࢩࣥࣉࣝࢫࢸࣥ MAX-PO(MULTI)ࠊࢽࢳࣞ㸦ᰴ㸧㸧 ࢆ⏝࠸ࠊ࣐ࢽࣗࣝᚑࡗ࡚ᛂࡉࡏࡓࠋⓎⰍࡣࠊAEC ᇶ㉁㸦ࢩࣥࣉࣝࢫ ࢸࣥ AEC ⁐ᾮࠊࢽࢳࣞ㸦ᰴ㸧㸧࡛ 5 ศ㛫ᛂࡉࡏࠊ࣐࣊ࢺ࢟ࢩࣜࣥ⁐ ᾮࢆ⏝࠸࡚᰾ᰁⰍࢆᐇࡋࡓࠋࡑࡢᚋࠊỌஂᶆᮏ⏝࣐࢘ࣥࢸࣥࢢ࣓ࢹ ࣒࢘㸦Ultramount Aqueous Permanent Mounting Mediumࠊࢲࢥࢪࣕࣃ ࣥ㸦ᰴ㸧㸧ࢆ⏝࠸࡚ᑒධࡋࡓࠋ 㸳 ㉸ᚤᙧែᏛⓗ᳨ᰝ ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾᳨ᰝࡋ࡚ࠊ࣐࣍ࣝࣜࣥᅛᐃࡋࡓ✵⭠ࢆࠊᐃἲᚑ࠸ ࢜ࢫ࣑࣒࢘㓟ᅛᐃࠊ࢚࣏ࣥᶞ⬡ໟᇙཬࡧ㉸ⷧษࡼࡾษ∦ࢆసᡂࡋࠊ㓑㓟 ࢘ࣛࢽ࣭ࣝࢡ࢚ࣥ㓟㖄ࡢ㔜ᰁⰍࢆࡋࡓࠋḟ࠸࡛ࠊ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾 (H-7500ࠊ(ᰴ)᪥❧〇సᡤ)ࢆ⏝࠸࡚ほᐹࡋࡓࠋ ࠙⤖ᯝࠚ 㸯 ⌮ゎ๗Ꮫⓗ᳨ᰝ ✵⭠ࡢ₢⭷㠃ࡽ┤ᚄ 3 cm ࡢ⭘⒗ࡀ✺ฟࡋࠊ⢓⭷㠃ࡶྠᵝࡢ⭘⒗ ࡀ」ᩘㄆࡵࡽࢀࡓ㸦ᅗ 1㸧ࠋ⭘⒗ࡢ㠃ࡣࠊ┤ᚄ 1 mm ࡢ㯤ⓑⰍᕢࡀ ከᩘ☜ㄆࡉࢀࡓ(ᅗ 2)ࠋ₢⭷ୗᗈ⠊࡞⾑⭘ࡀㄆࡵࡽࢀࡓࠋ Brachyspira 2015:5(1):16 Oizumi T, et al.: 15 図１ 空腸粘膜面 空腸粘膜に直径 3 cm 大の腫瘤が複数 認められる。 図２ ホルマリン固定後空腸割面 腫瘤の割面に直径 1 mm 大の黄白色 病巣（矢印）が多数認められる。 㸰 ⌮⤌⧊Ꮫⓗ᳨ᰝ ✵⭠ቨከⓎᛶ⫗ⱆ⭘ࡀㄆࡵࡽࢀࠊ⭘⒗≧ࢆ࿊ࡋ࡚࠸ࡓ㸦ᅗ 3㸧ࠋ⫗ⱆ⭘ ࡣቯṚࠊከ᰾ᕧ⣽⬊ࠊ㢮ୖ⓶⣽⬊ࢆ୰ᚰࡋ࡚ࠊእᒙࣜࣥࣃ⌫ࡢᾐ₶ࡀ ࡳࡽࢀࡓ㸦ᅗ 4㸧ࠋWS ᰁⰍᶆᮏ࡚ከ᰾ᕧ⣽⬊ཬࡧ㢮ୖ⓶⣽⬊ࡢ⣽⬊㉁ෆ ࣇ࣓ࣛࣥࢺ≧⣽⳦㸦㛗ࡉ⣙ 20 Ǎmࠊ┤ᚄ⣙ 0.3 Ǎm㸧ࡀᩓぢࡉࢀ㸦ᅗ 5㸧ࠊ ࢢ࣒ࣛ㝧ᛶࠊPAS ᛂ㝜ᛶཬࡧ㠀ᢠ㓟ᛶࢆ♧ࡋࡓ㸦⾲ 1㸧ࠋࡲࡓࠊ୍㒊ࡢ 図３ 空腸の病理組織像 図４ 空腸の病理組織像 空腸粘膜下組織に多発性に肉芽腫病 空腸粘膜下組織に多核巨細胞を伴う 変が認められる。 肉芽腫病変が認められる。 （HE 染色 Bar ＝ 500 μm） （HE 染色 Bar ＝ 50 μ m） Brachyspira 2015:5(1):17 Brachyspira 2015;5(1): 16 㡿ᇦ࡛ࡣ⢓⭷⾲ᒙࡽ⢓⭷ୗ⤌⧊ࡅ࡚ቯṚࠊ⥺⥔⣲ᯒฟࠊฟ⾑ཬࡧ✵ ⬊ᙧᡂࡀ࠶ࡾࠊ₽⒆ࢆᙧᡂࡋ࡚࠸ࡓࠋࡇࡢቯṚᕢ㎶⦕ࡣከ㔞ࡢ⣽⬊㢘ᗫ ≀ࡀࡳࡽࢀࠊቯṚᕢཬࡧቯṚᕢ㎶⦕ከᩘࡢࢢ࣒ࣛ㝧ᛶᱰ⳦ࠊࢢ࣒ࣛ㝜 ᛶᱰ⳦ࡀㄆࡵࡽࢀࡓࠋᢠ㓟⳦ࡣ᳨ฟࡉࢀ࡞ࡗࡓࠋ ⾲㸯 ∵ࡢ✵⭠࠾ࡅࡿዲ㖟ᛶࣇ࣓ࣛࣥࢺ≧⣽⳦ ሗ࿌⪅ 㒔㐨ᗓ┴ ရ✀ ᖺ㱋 ⮫ᗋ≧ ᵓ㐀䚷㛗䛥 䚷䚷䚷䚷┤ᚄ 䚷䚷䚷䚷ᙧែ ᮏ 㛗㔝 䝩䝹䝇䝍䜲䞁✀ 㻞ṓ㻢䞄᭶ ⭠㛢ሰ ⣙㻞㻜䃛㼙 ⣙㻜㻚㻟䃛㼙 䝣䜱䝷䝯䞁䝖≧ 㻻㼓㼍㼣㼍㻌㼑㼠㻌㼍㼘㻚䚷㻞㻜㻝㻜㻌㼇㻡㼉 ᗈᓥ 䝩䝹䝇䝍䜲䞁✀ 㻞ṓ㻝㻝䞄᭶ ⭠㔜✚ 㻞䡚㻝㻣䃛㼙 㻜㻚㻝䡚㻜㻚㻟䃛㼙 䝣䜱䝷䝯䞁䝖≧ 㠀ศ⠇ᛶ ዲ㖟ᛶ 㝧ᛶ 㝜ᛶ 㠀ᢠ㓟ᛶ 㻵㼎㼍㼞㼍㼗㼕㻌㼑㼠㻌㼍㼘㻚㻌㻌㻞㻜㻝㻝㻌㼇㻟㼉 ᗈᓥ 䝩䝹䝇䝍䜲䞁✀ 㻤ṓ ⭠㔜✚ 㻞䡚㻞㻤䃛㼙 㻜㻚㻞䡚㻜㻚㻟㻡䃛㼙 䝣䜱䝷䝯䞁䝖≧ ศ⠇䜢᭷䛧䚸 䜎䜜䛻ศᒱ ዲ㖟ᛶ 㝧ᛶ 㝧ᛶ 㠀ᢠ㓟ᛶ ዲ㖟ᛶ 䜾䝷䝮ᰁⰍ 㻼㻭㻿ᛂ ᢠ㓟ᛶ 㻳㼑㼚㻮㼍㼚㼗 㼍㼏㼏㼑㼟㼟㼕㼛㼚㻌㼚㼡㼙㼎㼑㼞 ᮍ᳨ᰝ 㻭㻮㻡㻟㻥㻤㻣㻡 㻭㻮㻠㻣㻞㻟㻟㻞 ዲ㖟ᛶ 㝜ᛶ 㝜ᛶ 㠀ᢠ㓟ᛶ 図５ 空腸の病理組織像 図６ フ ィラメント状細菌の透過型 複数の好銀性フィラメント状細菌 電子顕微鏡像 （矢印）が肉芽腫病変に認められる。 肉芽腫内に観察された長桿菌の細胞 （WS 染色 Bar ＝ 10 μm） 壁に厚いペプチドグリカン層が認め られる。 （Bar ＝ 100 nm） T, et al.: 17 BrachyspiraOizumi 2015:5(1):18 㸱 ච⤌⧊Ꮫⓗ᳨ᰝ ࣇ࣓ࣛࣥࢺ≧⣽⳦ࡣᢠ T. pallidum ᢠయࠊᢠ B. hyodysenteriae serotype-A ᢠయཬࡧᢠ B. pilosicoli ᢠయᑐࡋ࡚ࠊ࠸ࡎࢀࡶ㝧ᛶᛂࡣㄆ ࡵࡽࢀ࡞ࡗࡓࠋ 㸲 ㉸ᚤᙧែᏛⓗ᳨ᰝ ㏱㐣ᆺ㟁Ꮚ㢧ᚤ㙾ほᐹࡢ⤖ᯝࠊ⫗ⱆ⭘ෆ㛗ᱰ⳦ࡀᩓぢࡉࢀࡓࠋከᩘ ࡢ⳦ࡣኚᛶࡋ⣽⬊ቨࡣ᫂░࠶ࡿ࠸ࡣつ๎ᒙᯈ≧࡛࠶ࡗࡓࡀࠊ⛥ᵓ㐀 ࢆಖࡗࡓ⳦ࡀほᐹࡉࢀࠊࡇࡢࡼ࠺࡞⳦ࡣࢢ࣒ࣛ㝧ᛶ⳦ࡢ≉ᚩ࡛࠶ࡿཌ࠸࣌ ࣉࢳࢻࢢࣜ࢝ࣥᒙࢆ᭷ࡋ࡚࠸ࡓ㸦ᅗ 6㸧ࠋBrachyspira ᒓ⣽⳦࡞ࡢࢫࣆࣟ ࣮࣊ࢱࡳࡽࢀࡿ㍈⣒ࡣ☜ㄆࡉࢀ࡞ࡗࡓࠋ ࠙⪃ᐹࠚ ኚ㒊ෆ࡛ㄆࡵࡽࢀࡓዲ㖟ᛶࢢ࣒ࣛ㝧ᛶࣇ࣓ࣛࣥࢺ≧⣽⳦ࡀᙜヱ∵ ࡢ✵⭠⫗ⱆ⭘ࢆᘬࡁ㉳ࡇࡋࡓ⪃࠼ࡽࢀࡓࠋࡉࡽࡇࡢ⫗ⱆ⭘ᛶ⭘⒗ࡼ ࡾḟⓗ⭠㛢ሰࡀㄏⓎࡉࢀࡓ᥎ᐹࡉࢀࡓࠋ ᮏ㠀ᖖ㢮ఝࡋࡓሗ࿌ࡀ᪥ᮏ࡛ࡢࡳ 2 㹙3,5㹛ሗ࿌ࡉࢀ࡚࠸ࡿ 㸦⾲ 1㸧ࠋࡇࡢ 2 ࡣ⭠㔜✚࡛࠶ࡗࡓࡀᮏࡣ⭠㛢ሰ࡛࠶ࡗࡓⅬࡀ┦㐪ࡋ ࡚࠸ࡿࠋᮏᗈᓥ┴ࡢ 2 㹙3,5㹛ࡢྛࣇ࣓ࣛࣥࢺ≧⣽⳦ࡣࠊ⾲ 1 ࡢ࠾ࡾࠊศ⠇ࠊศᒱࠊࢢ࣒ࣛᰁⰍࠊPAS ᛂཬࡧ 16S rDNA 㑇ఏᏊ㓄 ิ࡛┦㐪ࡀ࠶ࡾࠊ3 ࡶ␗࡞ࡿ⣽⳦⪃࠼ࡽࢀࡓࠋࡇࡢࡇࡽ␗࡞ ࡿ⣽⳦࡛࠶ࡗ࡚ࡶ✵⭠㢮ఝࡢ⫗ⱆ⭘ᛶኚࢆᙧᡂࡋࠊ⭠㛢ሰ࠶ࡿ࠸ࡣ⭠ 㔜✚ࢆㄏⓎࡍࡿࡇࡀ⪃࠼ࡽࢀࡓࠋ ࡇࢀࡲ࡛ࡢࡇࢁ∵࡛ Brachyspira ᒓ⣽⳦ࡀศ㞳ࡉࢀࡓሗ࿌ࡣ࡞ࡃࠊ Brachyspira ᒓ⣽⳦ࡣࡽࡏࢇ≧ࠊࢢ࣒ࣛ㝜ᛶᱰ⳦࡛࠶ࡾࠊᮏࡣࣇࣛ ࣓ࣥࢺ≧ࠊࢢ࣒ࣛ㝧ᛶ࡛࠶ࡗࡓⅬࡀ┦㐪ࡋ࡚࠸ࡿࠋࡋࡋࠊࣇ࣓ࣛࣥࢺ ≧⣽⳦ࡣ⭠⤧ẟ╔ࡍࡿⅬ㹙8㹛ࡀ B. pilosicoli ࡞ࡽࡧ B. aalborgi ➼ 㢮ఝࡋ࡚࠾ࡾࠊගᏛ㢧ᚤ㙾࡛ࣞ࣋ࣝࡣࣇ࣓ࣛࣥࢺ≧⣽⳦ Brachyspira ᒓ⣽⳦ࢆ㚷ูࡍࡿࡢࡣ㞴ࡋ࠸ሙྜࡀ࠶ࡿࠋࡲࡓࠊᮏࡣච ᰁⰍ࡛㝧ᛶᛂࡣㄆࡵࡽࢀ࡞ࡗࡓࡀࠊ✵⭠⫗ⱆ⭘ࢆᘬࡁ㉳ࡇࡋࡓዲ㖟 ᛶ㠀ศ⠇ᛶࢢ࣒ࣛ㝜ᛶࣇ࣓ࣛࣥࢺ≧⣽⳦ࡢ⳦యࡢ୍㒊ࡀࠊචᰁⰍ࡛ᢠ Brachyspira 2015:5(1):19 Brachyspira 2015;5(1): 18 T. pallidum ᢠయࡸᢠ B. hyodysenteriae ᢠయ㝧ᛶᛂࢆㄆࡵࡓሗ࿌ࡉ ࢀ࡚࠸ࡿ[5]ࠋࡇࢀࡽࡢࣇ࣓ࣛࣥࢺ≧⣽⳦ࡣ⏕యእ࡛ᇵ㣴ࡍࡿࡇࡀ࡛ࡁ ࡎࠊศ㢮ࡸཎᛶࡶゎࡗ࡚࠸࡞࠸ࡓࡵࠊBrachyspira ᒓ⣽⳦ࡢ㚷ูࡀ㔜 せ⪃࠼ࡽࢀࡓࠋ ᚋࡣ㢮ఝࡍࡿ㛵ࡍࡿ▱ぢࢆ✚ࡋࠊࡇࢀࡽࣇ࣓ࣛࣥࢺ≧⣽⳦ ࡢ⣽⳦Ꮫⓗ≉ᚩࢆ᫂ࡽࡍࡿᚲせࡀ࠶ࡿࠋࡲࡓࠊࣇ࣓ࣛࣥࢺ≧⣽⳦ࡢ ឤᰁ※ࠊឤᰁ⤒㊰ࠊ⏕యࡢᙳ㡪ࡘ࠸࡚ヲ⣽ㄪࡿᚲせࡀ࠶ࡿࠋ ㅰ㎡ ච⤌⧊Ꮫⓗ᳨ᰝ࠶ࡓࡾᚚ༠ຊ㡬࠸ࡓࠊ(⊂) ㎰ᴗ࣭㣗ရ⏘ᴗᢏ⾡ ⥲ྜ◊✲ᶵᵓື≀⾨⏕◊✲ᡤࡢᑠᯘᵝࠊᔱ⏣ᜨ⨾ᵝ῝ࡃឤㅰ⏦ࡋୖࡆ ࡲࡍࠋ 㐃⤡㈐௵⪅㸸Ἠ༟ஓ ࠛ390-0851 㛗㔝┴ᯇᮏᕷᓥෆすᕝཎ 6931 㛗㔝 ͤ ┴ᯇᮏᐙ␆ಖ⾨⏕ᡤ TEL:0263-47-3223㸹FAX:0263-47-0101; E-mail address: [email protected] 㸦ㄽᩥཷ㸹㸰㸮㸯㸲ᖺ㸶᭶㸯㸯᪥ࠊཷ⌮㸰㸮㸯㸲ᖺ㸯㸮᭶㸰㸶᪥㸧 ᘬ⏝ᩥ⊩ 1 Gong J, Si W, Forster RJ, et al. 2007. 16S rRNA gene-based analysis of mucosa-associated bacterial community and phylogeny in the chicken gastrointestinal tracts: from crops to ceca. FEMS Microbiol Ecol 59: 147-57. 2 Hoskins JD, Henk WG, Abdelbaki YZ. 1982. Scanning electron microscopic study of the small intestine of dogs from birth to 337 days of age. Am J Vet Res 43: 1715-20. 3 Ibaraki Y, Shibahara T, Kobayashi H, et al. 2011. Multifocal granulomatous jejunitis associated with an argyrophilic T, et al.: 19 BrachyspiraOizumi 2015:5(1):20 gram-positive segmented filamentous bacterium in a Holstein cow. J Comp Pathol 145: 118-21. 4 Lowden S, Heath T. 1995. Segmented filament bacteria associated with lymphoid tissues in the ileum of horses. Res Vet Sci 59: 272-4. 5 Ogawa K, Shibahara T, Kobayashi H, et al. 2010. Jejunal granuloma associated with an argyrophilic gram-negative non-segmented filamentous bacterium in a Holstein cow. J Comp Pathol 143: 52-6. 6 Pearson GR, McNulty MS, McCracken RM, et al. 1982. Scanning electron microscopic observations of segmented filamentous bacteria in the small intestine of domestic fowl. Vet Rec 111: 366-7. 7 Sanford SE. 1991. Light and electron microscopic observations of a segmented filamentous bacterium attached to the mucosa of the terminal ileum of pigs. J Vet Diagn Invest 3: 328-33. 8 Smith TM. 1997. Segmented filamentous bacteria in the bovine small intestine. J Comp Pathol 117: 185-90. 9 Snel J, Heinen PP, Blok HJ, et al. 1995. Comparison of 16S rRNA sequences of segmented filamentous bacteria isolated from mice, rats, and chickens and proposal of ‘Candidatus arthromitus’. Int J Syst Bacteriol 45: 780-2. Regular paper Jejunal granuloma associated with argyrophilic, gram-positive filamentous bacteria in a Holstein cow Authors: Takuya Oizumi1ͤ, Sayuri Yahikozawa2, Hanae Inagaki㸱, Makoto Haritani㸱, Tomoyuki Shibahara㸱 Institution: Matsumoto Livestock Hygiene Service Center1, Iida Livestock Hygiene Service Center2, National Institute of Animal Health㸱 Brachyspira 2015:5(1):21 Brachyspira 2015;5(1): 20 Abstract A length of obstructed jejunum was resected surgically from a 30-month-old Holstein cow. Several masses were grossly detected in the jejunal wall, and microscopically, the masses consisted of multifocal granuloma of epithelioid cells and occasional multinucleated giant cells. Although acid-fast bacilli were not found, some argyrophilic, gram-positive, and periodic acid Schiff-negative, filamentous bacteria were detected in the cytoplasm of the epithelioid cells and multinucleated giant cells. This argyrophilic gram-positive filamentous bacterium appears to be responsible for jejunal multifocal granulomas, which resulted in obstructive ileus in this cow. Brachyspira may be a candidate of a list of differential diagnosis of this filamentous bacterium, which causes such rare intestinal lesions. Key words: Gram-positive filamentous bacteria; granuloma; jejunum ͤ Corresponding author: Takuya Oizumi, Matsumoto Livestock Hygiene Service Center, 6931 Shimauchi-nishigawara, Matsumoto, Nagano, 390-0851, Japan TEL:+81-263-47-3223㸹FAX:+81-263-47-0101; E-mail address: [email protected] (This paper was received on August 11, 2014, and accepted on October 28, 2014.) Brachyspira 2015:5(1):22 Shibahara T, et al.: 21 ᩍ⫱ㅮᗙ Brachyspira ㇜㉥⑩ Ⱚཎᖾ 1ͤࠊ✄ᇉ⳹⤮ 1,2 1(⊂) ㎰ᴗ࣭㣗ရ⏘ᴗᢏ⾡⥲ྜ◊✲ᶵᵓື≀⾨⏕◊✲ᡤ ែ◊✲㡿ᇦࠊ 2 ᾏ㐨▼⊁ᐙ␆ಖ⾨⏕ᡤ せ⣙ Brachyspira hyodysenteriae Brachyspira pilosicoli ࡣ㇜㉥⑩ࡸ㇜⤖ ⭠ࢫࣆ࣮ࣟ࣊ࢱࡢཎᅉ࡞ࡿࠋ㏆ᖺࠊ㠀ᆺⓗ࡞ᙉș⁐⾑ᛶศ㞳ᰴࡢศ Ꮚ⏕≀Ꮫⓗゎᯒ(nox 㑇ఏᏊ㓄ิ 16S ribosomal RNA 㑇ఏᏊ㓄ิ)⏕ Ꮫⓗゎᯒࡼࡾࠊ᪂ࡋ࠸ Brachyspira ࡀ࣓ࣜ࢝ࠊ࢝ࢼࢲཬࡧ࣮ࣚࣟࢵ ࣃ ㅖ ᅜ ࡛ ᣑ ࡀ ࡗ ࡚ ࠸ ࡿ ࡇ ࡀ ᫂ ࡽ ࡞ ࡾ ࠊ ᬻ ᐃ ✀ ྡ “Brachyspira hampsonii ”ࡀᥦၐࡉࢀࡓࠋ“B. hampsonii ”ࡣࠊ2 ࡘࡢࢡ࣮ࣞࢻ (“B. hampsonii ” clade I clade II) ศࡅࡽࢀࠊ࠸ࡎࢀࡶ B. hyodysenteriae ࡼࡿ㇜㉥⑩㢮ఝࡋࡓ≧ኚࢆᘬࡁ㉳ࡇࡍࠋᮏ✏࡛ࡣࠊࡇࡢ͆B. hampsonii ”ࢆ୰ᚰ Brachyspira ࡢཎᛶศᕸ㛵ࡋ࡚⌧ᅾࡲ࡛ࡢ▱ ぢࢆグ㏙ࡍࡿࠋ ࣮࣮࢟࣡ࢻ:͆Brachyspira hampsonii ”; Brachyspira hyodysenteriae; ㇜ ㉥⑩. Brachyspira 2015;5(1): 22 Brachyspira 2015:5(1):23 ࠙⥴ゝࠚ Brachyspira spp. ࡣ㇜ࡢ⣅౽ࡽࡋࡤࡋࡤ᳨ฟࡉࢀࠊࡇࡢ࠺ࡕᝈ㛵 ࢃࡿ௦⾲ⓗ࡞ࡶࡢࡋ࡚ Brachyspira hyodysenteriae Brachyspira pilosicoli ࡀ࠶ࡿࠋ ㏆ᖺࠊBrachyspira 㛵ࡍࡿ᪂ࡓ࡞⳦✀ࡢᥦၐࡑࡢཎᛶ㛵ࡍࡿ▱ ぢࡀከࡃሗ࿌ࡉࢀ࡚࠸ࡿ(⾲ 1㸧ࠋ“Brachyspira hampsonii ” [7]ࡣ 2012 ᖺ ᥦၐࡉࢀࡓ᪂ࡋ࠸✀࡛࠶ࡿࠋ㇜㉥⑩㢮ఝࡋࡓ⢓⾑ᛶୗ⑩ࢆ࿊ࡋࡓ㇜ ࡽศ㞳ࡉࢀࠊ௦⾲ᰴ(strain 30446 [38])ࢆ⏝࠸ࡓᐇ㦂ឤᰁ࡛ࠊ㇜ኚࡀ ⌧ࡉࢀࡿࡇࡀ᫂ࡽ࡞ࡗ࡚࠸ࡿ[4]ࠋ“B. hampsonii ”ࡼࡿែ B. hyodysenteriae ㉳ᅉࡍࡿ㇜㉥⑩[40]ࢆ༊ูࡍࡿࡇࡣ㠀ᖖ㞴ࡋ ࠸ࠋ ᮏ✏࡛ࡣࠊ“B. hampsonii ”ឤᰁ㛵ࡍࡿ⌧ᅾࡲ࡛ࡢ▱ぢࢆࡲࡵࡿ ࡶࠊᛶ㚷ᐃ㔜せ࡞ࡿ Brachyspira ឤᰁ㢮ఝࡘ࠸࡚ グ㏙ࡍࡿࠋ ࠙Brachyspira ᒓ⳦ࠚ Brachyspira ࡢྡ⛠ࡣࠊ ͆brachy͇ 㸦▷࠸㸧ࠊ ͆speira͇ 㸦ࢥࣝ㸧⏤᮶ࡍ ࡿࠋBrachyspira ࡣࢫࣆ࣮ࣟ࣊ࢱ㛛ᒓࡋࠊࢢ࣒ࣛ㝜ᛶࠊࡽࡏࢇ≧ࡢࠊ㧗 ࠸㐠ືᛶࢆࡶࡘ᎘Ẽᛶ⣽⳦࡛࠶ࡿ[12, 40]ࠋBrachyspira ᒓࡣ⌧ᅾ 7 ⳦✀ (B. aalborgi ࠊ B. alvinipulli ࠊ B. hyodysenteriae ࠊ B. innocens ࠊ B. intermediaࠊB. murdochii ཬࡧ B. pilosicoli )ࡀṇᘧㄆࡵࡽࢀ࡚࠸ࡿ(⾲ 1㸧ࠋࡇࢀࡽ“B. hampsonii ”“B. suanatina”ࢆຍ࠼ࡓ 9 ࡘࡢᶆ‽ᰴࡀ ATCC Ⓩ㘓ࡉࢀ࡚࠸ࡿࠋࡲࡓࠊṇᘧ࡛ࡣ࡞࠸ࡶࡢࡢ“B. canis”[15]ࠊ“B. corvi ”[16]ࠊ“B. ibaraki ”[28]ཬࡧ“B. pulli ”[10]࡞ࡶሗ࿌ࡉࢀ࡚࠸ࡿࠋ ᙅ ᙅ ᙉ ᙅ ᙅ ᙅ ᙅ ᙉ ᙉ B.aalborgi B.alvinipulli B. hyodysenteriae B.innocens B.intermedia B.murdochii B.pilosicoli “B.hampsonii” “B.suanatina” 2) 䠇䠖㝧ᛶ 䠉䠖 㝜ᛶ 䠇/䠉䠖㝧 ᛶ/㝜ᛶ䛜ᰴ䛻 䜘䛳䛶␗䛺䜛 ATCC䛻Ⓩ㘓䛥 䜜䜜䛔䜛䜒䛾 䠍䠅 ɴ⁐⾑ᛶ ⳦✀ྡ䠍䠅 ㇜䚸䝬䜺䝰 ㇜䛾㇜㉥⑩ᵝ≧ 䠇 䠉 ㇜䛾㇜㉥⑩ᵝ≧ ㇜䚸䝝䜲䜲䝻䜺䞁䚸 䜺䝏䝵䜴䚸䝬䜺䝰 䠉 䠇 䠉 䠇 䠉 䠉 䠉 䠇 䠉 䠉 䠉 䠉 䠇 グ㍕䛺䛧 䠉 䠉 䠉 䠇 䠇/䠉 䠇 䠉 ⭏ॆᆖⲴᙗ⣦2) 䜲䞁䝗䞊䝹⏘ ɲͲ䜾䝹䝁䝅 楔⯧愠≈㯜↮妋 ⏕ 䝎䞊䝊 䠇 䠉 䠉 䠉 㭜䛾ୗ⑩䚸⏘༸పୗ䛚䜘䜃 ༸Ẇ䛾⣅౽ởᰁ ㇜㉥⑩ 㭜䛾ୗ⑩䚸⏘༸పୗ䛚䜘䜃 ༸Ẇ䛾⣅౽ởᰁ ே⭠⟶䝇䝢䝻䝦䞊䝍 䛺ྡ ㇜⤖⭠䝇䝢䝻䝦䞊䝍 専ˣ洷ˣ䉔ˣ楔ˣ 㭜䛾ୗ⑩䚸⏘༸పୗ䛚䜘䜃 Ṣˣ暲攟栆 ༸Ẇ䛾⣅౽ởᰁ ㇜䚸㭜䚸䝷䝑䝖 ㇜䚸㭜 専ˣ洷ˣ䉔ˣ楔 ㇜䚸㭜䚸䝷䝑䝖䚸䝬䜴 䝇䚸䝬䜺䝰䚸䜺䝏䝵䜴 㭜䚸≟䚸䜺䝏䝵䜴 ே䚸㟋㛗㢮 ື≀ ⾲1 Brachyspira 䛾ཎᛶ Brachyspira 2015:5(1):24 䠉 䠉 䠇 䠉 䠉 䠇 䠉 䠇/䠉 ɲͲ䜺䝷䜽䝖䝅 䝎䞊䝊 䠉 䠇 䠇/䠉 䠉 䠇 䠇 䠇 䠇 䠇 ɴͲ䜾䝹䝁䝅 䝎䞊䝊 䠉 [34] [7] [32] [32] [32] [32] [32] [10] [6] ᩥ⊩ Shibahara T, et al.: 23 Brachyspira 2015;5(1): 24 Brachyspira 2015:5(1):25 ࠙㇜㉥⑩ࠚ ㇜࠾࠸࡚ࠊBrachyspira ᒓ⳦ࡼࡿ᭱ࡶ㔜せ࡞ࡣࠊ㇜㉥⑩࡛࠶ࡿࠋ ㇜㉥⑩ࡣ 1921 ᖺึࡵ࡚ሗ࿌ࡉࢀࠊᙜࡣཎᅉ⳦ࡀ࡛᫂࠶ࡗࡓࠋ1971 ᖺࠊTreponema hyodysenteriae (ᚋࡢ B. hyodysenteriae)ࡀཎᅉ⣽⳦࡛ ࠶ࡿࡇࡀ᫂ࡽ࡞ࡗࡓ[14, 44]ࠋ B. hyodysenteriae ࡣș⁐⾑ᛶࢆ♧ࡋࠊࡇࢀࡀཎᛶࡢᣦᶆࡉࢀࡿࠋ B. hyodysenteriae ࡢ⁐⾑ẘ haemolysin ࡣࠊ⭠⟶ࡢ⢓⭷ୖ⓶⣽⬊㔜ᗘࡢ 㞀ᐖࢆ࠼ࡿࡇࡀࠊ↓⳦㇜ࡢ⤖⣻⭠⟶࣮ࣝࣉヨ㦂࡛☜ㄆࡉࢀ࡚࠸ࡿ[2, 24]ࠋ B. hyodysenteriae ࡣ㇜⢓⾑ᛶୗ⑩ཬࡧ⭠⅖ࢆᘬࡁ㉳ࡇࡍࠋⓎ⏕㎰ ሙ࡛ࡣࠊ㧗࠸Ṛஸ⋡ࠊ㣫ᩱຠ⋡ཬࡧᡂ㛗⋡ࡢపୗࡀࡳࡽࢀࠊᢠ⏕≀㉁⒪ ࡢࡓࡵ⏕⏘ࢥࢫࢺࡢቑࢆᣍࡃࠋࡇࡢࡼ࠺㇜㉥⑩ࡣከ࡞⤒῭⿕ᐖࢆࡶ ࡓࡽࡍࡇࡽࠊୡ⏺ྛᅜࡢ㣴㇜⏘ᴗ࠾࠸࡚㔜せ࡞ࡢ୍ࡘࡉࢀ࡚ ࠸ࡿࠋ ࠙ᙅș⁐⾑ᛶ Brachyspira ࡼࡿែࠚ B. hyodysenteriae ௨እࡢࠊᙅș⁐⾑ᛶ Brachyspira ࡢᐙ␆ᑐࡍࡿ ཎᛶࡢ୍㒊ࡀ᫂ࡽ࡞ࡗ࡚ࡁ࡚࠸ࡿ[22]ࠋ ᙜึࠊᙅș⁐⾑ᛶ Brachyspira ࡣ∦ඹ⏕⳦⪃࠼ࡽࢀࠊࡇࡢࡓࡵ B. innocens ྡࡉࢀࡓࠋࡋࡋࠊᙅș⁐⾑ᛶ Brachyspira ࡼࡿᗄࡘ ࡢⓎࡀሗ࿌ࡉࢀࠊ୍㒊ࡢᙅș⁐⾑ᛶ Brachyspira ࡀ㇜ᑐࡋཎᛶ ࢆ♧ࡍࡇࡀ♧၀ࡉࢀ࡚࠸ࡿ[30]ࠋࡇࢀࡽᙅș⁐⾑ᛶᰴࡘ࠸࡚ࡢࠊ DNA-DNA hybridization ➼ࢆ⏝࠸ࡓヲ⣽࡞ㄪᰝ࡛ࠊࡉࡽ 3 ࡘࡢᙅș⁐ ⾑ᛶ⳦✀ࡀྡࡉࢀࡓ(B. intermediaࠊB. murdochii ཬࡧ B. pilosicoli ) [42, 45, 46]ࠋ ㏆ᖺࡢ◊✲ࡼࡾࠊB. pilosicoli ࡣ㇜㠀ฟ⾑ᛶࡢୗ⑩㣫ᩱຠ⋡ࡢప ୗࢆ≉ᚩࡍࡿ㇜⤖⭠ࢫࣆ࣮ࣟ࣊ࢱࢆᘬࡁ㉳ࡋࠊᵝࠎ࡞့ஙື≀㸦ேࠊ 㟋㛗㢮ࠊ≟ཬࡧ㤿㸧ࡢୗ⑩ࡶ㛵ࡋ࡚࠸ࡿࡇࡀ᫂ࡽ࡞ࡗ࡚࠸ࡿ[13, 15, 20, 46]ࠋࡉࡽࠊB. alvinipulliࠊB. intermedia ཬࡧ B. pilosicoli ࡣࠊ Shibahara T, et al.: 25 Brachyspira 2015:5(1):26 㭜ୗ⑩ࡸ⏘༸పୗཬࡧ༸Ẇࡢ⣅౽ởᰁ(fecal staining of eggshells)ࢆᘬ ࡁ㉳ࡇࡍ⪃࠼ࡽࢀ࡚࠸ࡿ[17]ࠋ୍᪉ࠊB. murdochii ࡢ㇜ࡢཎᛶࡣ᫂ ☜࡛ࡣ࡞࠸ࠋ୧✀ࡣୗ⑩ࢆ࿊ࡋࡓ㇜ࡽศ㞳ࡉࢀ࡚࠸ࡿࡀࠊᐇ㦂ឤᰁ࠾ ࡅࡿ⮫ᗋ≧ࡣ㍍ᗘཪࡣ↓≧࡛ࠊࡑࡢⓎࡣከࡃࡢ⳦ᩘࡀᚲせ࡛࠶ࡿ [19, 21]ࠋ ࠙Brachyspira ᒓ⳦ࡢྠᐃࠚ Brachyspira ᒓ⳦ࡢྠᐃ⏝࠸ࡽࢀࡿ୍⯡ⓗ࡞᪉ἲࡣࠊศ㞳ᇵ㣴ࠊ⾲⌧ ᆺศᯒ⏕Ꮫⓗศᯒ [11]ࠊ✀≉␗ⓗ࡞࣏࣓࣮ࣜࣛࢮ㐃㙐ᛂ(PCR) NADH oxidase (nox)㑇ఏᏊ㓄ิゎᯒ[1, 35]࡛࠶ࡿࠋ௦⾲ⓗ࡞ Brachyspira ᒓ⳦ཬࡧᬻᐃ⳦✀ࡢ⏕Ꮫᛶ≧ࢆ⾲㸯♧ࡍࠋྛ⳦✀ࡢ⏕Ꮫᛶ≧ࡣሗ࿌ ࡼࡗ࡚ⱝᖸࡢ┦㐪ࡀ࠶ࡾࠊ⳦ᰴࡼࡿእࡶ࠶ࡿࡇࡽࠊྠᐃࡢ㝿 ࡣ㑇ఏᏊ᳨ᰝࢆྵࡵࡓ⥲ྜⓗ࡞ุ᩿ࡀᚲせ࡛࠶ࡿࠋ Brachyspira ᒓ⳦ࡢ 16S ribosomal RNA (rRNA)㑇ఏᏊ㓄ิࡣ┦ྠᛶࡀ㧗࠸ࡓࡵࠊ✀ࡢྠᐃ ࡣྥ࠸࡚࠸࡞࠸ [37]ࠋ୍᪉ࠊnox 㑇ఏᏊ㓄ิࡣࠊ⳦✀㛫࡛㑇ఏᏊࡢከᵝᛶ ࡀ㧗࠸ࡇࡽࠊࡼࡾከࡃࡢ Brachyspira ✀ࢆ༊ูࡍࡿࡇࡀ࡛ࡁࡿ[37]ࠋ ㏆ᖺ࡛ࡣࠊ࣐ࢺࣜࢵࢡࢫᨭ࣮ࣞࢨ࣮⬺㞳࢜ࣥï㣕⾜㛫ᆺ㉁㔞ศ ᯒ ἲ ⣽ ⳦ ྠ ᐃ ࢩ ࢫ ࢸ ࣒ (Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) bacterial identification systems)ࡼࡿྠᐃࡢ⢭ᗘࡑࡢ㎿㏿ᛶࡀ᳨ドࡉࢀ࡚࠸ࡿ [47]ࠋ ࠙͆B. suanatina”“B. hampsonii ”ࠚ ㏆ᖺࠊཎᛶࢆࡶࡘ⪃࠼ࡽࢀࡿ᪂ࡓ࡞ Brachyspira ࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋ 2007 ᖺࠊࢫ࢙࣮࢘ࢹࣥࢹ࣐࣮ࣥࢡࡢ㇜ࠊB. hyodysenteriae ࡣ㑇 ఏⓗ␗࡞ࡿࠊᙉș⁐⾑ᛶࢆ♧ࡍ Brachyspira ࡀ☜ㄆࡉࢀࡓࠋࡇࢀࡽࡢᰴ ࡣ㞳ங㇜ࢆ⏝࠸ࡓឤᰁヨ㦂࡛㇜㉥⑩ྠᵝࡢୗ⑩⭠⅖ࡀ☜ㄆࡉࢀࠊᬻ ᐃྡࡋ࡚͆B. suanatina”ࡀᥦၐࡉࢀࡓ[34](⾲ 1㸧ࠋ 2000 ᖺ௦༙ࡤࡽࠊ⡿ࡢ㣴㇜ሙ࠾࠸࡚ࠊ㇜ࡢ⢓⾑ᛶୗ⑩ཬࡧ⭠ ⅖ࡢ⯆ࡀㄆࡵࡽࢀࡿࡼ࠺࡞ࡗࡓ[8]ࠋㄪᰝࡼࡾࠊB. hyodysenteriae Brachyspira 2015;5(1): 26 Brachyspira 2015:5(1):27 ࡼࡿࡢቑຍࡶࠊ᪤▱ࡢ✀ࡣ␗࡞ࡿ“㠀ᆺⓗ”Brachyspira ᒓ⳦㉳ᅉࡍࡿࡶቑ࠼࡚࠸ࡿࡇࡀ᫂ࡽ࡞ࡗࡓ[8]ࠋࡇࢀࡽࡢ ࡢ࠺ࡕࠊ⡿ᅜ࣑ࢿࢯࢱ⋇་デ᩿◊✲ᡤ㏦ࡽࢀࡓศ㞳ᰴࢆࠊnox 㑇ఏᏊ 16S rRNA 㑇ఏᏊࡢࢾࢡࣞ࢜ࢳࢻ⣔⤫ᶞゎᯒ࡛ศᯒࡋࡓࡇࢁࠊࡢ Brachyspira ᒓ⳦ࡢ㛫ࡁ࡞㑇ఏⓗ┦㐪ࡀ࠶ࡿࡇࡀุ᫂ࡋࡓࠋࡇࡢ ศ㞳ᰴᑐࡋ͆B. hampsonii ͇࠸࠺ྡ⛠ࡀᥦၐࡉࢀࡓࠋnox 㑇ఏᏊ㓄 ิࡢゎᯒࡼࡾࠊ“B. hampsonii ”ࡣ 2 ࡘࡢ␗࡞ࡿ⣔⤫Ⓨ⏕ࢡ࣮ࣞࢻ㸦clade I ϩ㸧ࡽ࡞ࡿࡇࡀ᫂ࡽ࡞ࡗ࡚࠸ࡿࠋ୧ࢡ࣮ࣞࢻࡣ࠸ࡎࢀࡶࠊ᪤ ▱ࡢ Brachyspira ࡣ⊂❧ࡋࡓࢡࣛࢫࢱ࣮ࢆᙧᡂࡋ࡚࠾ࡾࠊࢡ࣮ࣞࢻ㛫ࡢ ┦ྠᛶࡣ 96㸣࡛࠶ࡗࡓࠋ[7]ࠋศ㞳ᰴ 20 ᰴࡢ⏕Ꮫᛶ≧ࢆ᳨ᰝࡋࡓࡇࢁࠊ ࡍ࡚ࡢศ㞳ᰴ㸦cladeϨ17 ᰴࠊcladeϩ3 ᰴ㸧࡛ࣥࢻ࣮ࣝ㝜ᛶࠊ㤿ᒀ㓟 ຍỈศゎ㝜ᛶࠊȘ-ࢢࣝࢥࢩࢲ࣮ࢮ㝜ᛶࠊȘ̺࢞ࣛࢡࢺࢩࢲ࣮ࢮ㝜ᛶ࡛ࠊș ̺ࢢࣝࢥࢩࢲ࣮ࢮࡘ࠸࡚ࡣ cladeϨࡢ 17 ᰴ୰ 16 ᰴࡀ㝧ᛶࠊcladeϩࡢ 3 ᰴ୰ 2 ᰴࡀ㝜ᛶ࡛࠶ࡗࡓ[7]ࠋ ࠙ྛᅜ࠾ࡅࡿ“B. hampsonii ”ࡢ᳨ฟ≧ἣࠚ ࢝ ࢼ ࢲ ࡛ ࡣ 2011 ᖺ ࠊ ୗ ⑩ ࢆ ࿊ ࡋ ࡓ ㇜ ࡢ ⣅ ౽ ⤌ ⧊ ࡽ “B. hampsonii ” clade I (strain 30599) ࡀ᳨ฟࡶࡋࡃࡣศ㞳ࡉࢀ࡚࠸ࡿ [9]ࠋ ࡲࡓࠊ࢝ࢼࢲࡢࢧࢫ࢝ࢳ࡛ࣗ࣡ࣥᖺ㱋ࡢ㇜ࡢ┤⭠ࢫ࣡ࣈࢆㄪᰝࡋࡓࡇ ࢁ B. murdochii ࡀከࡃ᳨ฟࡉࢀࠊB. pilosicoliࠊB. innocens ຍ࠼࡚“B. hampsonii ”ࡶ᳨ฟࡉࢀ࡚࠸ࡿ[33]ࠋ ࣚ ࣮ ࣟ ࢵ ࣃ ࡛ ࡣ ࠊ ࣋ ࣝ ࢠ ࣮ ࡽ ࢻ ࢶ ㍺ ධ ࡉ ࢀ ࡓ ㇜ ࡽ “B. hampsonii ” clade I ࡀ 3 ᰴศ㞳ࡉࢀࠊ୍㢌ࡢ㇜࡛ࡣ୰➼ᗘࡢኚࡀ☜ㄆࡉ ࢀ࡚࠸ࡿ[36]ࠋ࣋ࣝࢠ࣮࡛ࡣࠊࢳ࢙ࢥࡽ㍺ධࡉࢀࡓ㇜ࡽ“B. hampsonii ” clade I ࡀศ㞳ࡉࢀ࡚࠸ࡿ[25]ࠋ ࠙“B. hampsonii ”ࡢཎᛶࠚ ࢝ࢼࢲࡢ㇜ࡽศ㞳ࡉࢀࡓ“B. hampsonii ” clade II 30446 ᰴࢆᐇ㦂ឤ ᰁࡉࡏࡓ 13 㐌㱋㇜࠾࠸࡚ࠊ⢓⾑ᛶୗ⑩⭠⅖ࡀ⌧ࡉࢀ࡚࠸ࡿ[38]ࠋ Burrough ࡽࡣ࣐࢘ࢫ[3]㇜[4]࡛⢓⾑ᛶୗ⑩⭠⅖ࢆ⌧ࡋ࡚࠸ࡿࠋᐇ Brachyspira 2015:5(1):28 Shibahara T, et al.: 27 㦂⏝࠸ࡓᙉ ǃ ⁐⾑ᛶᰴࡣᙜึ PCR ࡛ B. intermedia ࡉࢀ࡚࠸ࡓࡀ [41]ࠊᚋ“B. hampsonii ” clade ϩྠᐃࡉࢀ࡚࠸ࡿ[25]ࠋWilbert ࡽࡢ ᐇ㦂࡛ࡣࠊ“B. hampsonii ” clade ϩศ㞳ᰴ B. hyodysenteriae ᰴࢆ 6 㐌 㱋ࡢ㇜⤒ཱྀឤᰁࡉࡏࡓࡇࢁࠊኚࡢᙉࡉࠊศᕸࠊ⤖⭠ࡢ࣒ࢳࣥศἪ㔞 ࡁ࡞㐪࠸ࡣㄆࡵࡽࢀ࡚࠸࡞࠸[48]ࠋ ࡲࡓࠊ“B. hampsonii ” clade I (strain 30599) ࢆ⫶ෆᢞࡋࡓ㇜ࡢ 11 㢌୰ 9 㢌ࡀⓎࡋࠊ⢓⾑ᛶୗ⑩ࢆᘬࡁ㉳ࡇࡍࡇࡀ☜ㄆࡉࢀ࡚࠸ࡿ[9]ࠋࡇ ࢀࡽࡢࡇࡽࠊ“B. hampsonii ” clade I ୪ࡧ clade II ࡶ㇜㉥⑩ᵝࡢ ែࢆᘬࡁ㉳ࡇࡍࡇࡀ᫂ࡽ࡞ࡗ࡚࠸ࡿࠋ ࠙“B. hampsonii ”ࡢಖ⳦ື≀ࠚ “B. hampsonii ”ࡢ㉳※㇜௨እࡢಖ⳦ື≀ࡘ࠸࡚ࡣᮍࡔ᫂࡞Ⅼࡀ ከ࠸ࡀࠊ࣭࢞ࣥ࢝ࣔ┠ ὀពࡍࡿᚲせࡀ࠶ࡿࠋ㔝⏕ࡢ࣐࢞ࣔࡣᐙ␆ࡼ ࡃㄆࡵࡽࢀࡿᩘ✀㢮ࡢ Brachyspira ࡢ࢟ࣕࣜ࡞ࡿࡇࡀ▱ࡽࢀ࡚࠸ ࡿ[18]ࠋỈ㫽ࡣࠊ㣧Ỉ㎰స≀ࡢ⣅౽ởᰁ㔜せ࡞ᙺࢆᢸ࠺⪃࠼ࡽࢀࠊ ᒇእࡢᐙ␆ࡶ᥋ゐࡍࡿྍ⬟ᛶࡀ࠶ࡿ[23]ࠋ≉࣭࢞ࣥ࢝ࣔ┠ࡢΏࡾ㫽ࡣ Brachyspira ࢆ㛗㊥㞳Ώࡗ࡚㐠ࡪࡇࡽࠊ␗࡞ࡿᅜࡢᐙ␆ᑐࡋឤᰁ ※࡞ࡿྍ⬟ᛶࡀ࠶ࡿࠋ ࡲࡓࠊ㔝⏕ࡢ࣐࢞ࣔࡽศ㞳ࡉࢀࡓ B. pilosicoli ศ 㞳ᰴࡢ ǃ-lactam ឤཷᛶࡀపୗࡋ࡚࠸ࡿࡢሗ࿌ࡀ࠶ࡾ[19]ࠊᐙ␆ࡢఏ ࣜࢫࢡࢆྵࡵ࡚ὀពࡀᚲせ࡛࠶ࡿࠋࢫ࣌ࣥ࠾ࡅࡿಖ⳦≧ἣㄪᰝ࡛ࡣࠊ ᏘࡢΏࡾ㫽㸦ࣁࣟ࢞ࣥࠊ࢞ࢳࣙ࢘ཬࡧ࣐࢞ࣔ㸧ࡢ⣅౽ 205 ࢧࣥࣉࣝ ୰ 51 ࢧࣥࣉࣝࡽ Brachyspira ࡀศ㞳ࡉࢀ࡚࠸ࡿࠋࡑࡢෆヂࡣ B. pilosicoli (5.9%)ࠊ B. alvinipulli (11.8%)ࠊ͆B. hampsonii ” (19.6%)ࠊB. murdochii (23.5%)ཬࡧ B. innocens (39.2%)࡛࠶ࡾࠊࡇࢀࡽࡢ㫽ࡀ“B. hampsonii ”ࡢಖ⳦ື≀࡞ࡿࡇࡀ᫂ࡽ࡞ࡗࡓࠋnox 㑇ఏᏊ㓄ิࡢ⣔ ⤫ᶞゎᯒ࡛ ͆B. hampsonii ”ࡣࠊB. murdochii B. innocens ㏆⦕࡛࠶ ࡗࡓ [26]ࠋࡲࡓࠊ2011 ᖺࡣࠊ࢝ࢼࢲᴟᅪࡢࣁࢡ࢞ࣥ㸦࢝ࣔ┠࢝ࣔ⛉ ࣐࢞ࣥᒓ㸧ࡢࢡࣟ࢝ࢫ࣡ࣈ 170 ࢧࣥࣉࣝ୰ 15 ࢧࣥࣉࣝ (8.8 %) ࡽ͆B. hampsonii ”ࢆྵࡴ Brachyspira ᒓ⳦ࡀศ㞳ࡉࢀ࡚࠸ࡿ[39]ࠋ Brachyspira 2015;5(1): 28 Brachyspira 2015:5(1):29 ࠙“B. hampsonii ” ࡢྠᐃࠚ ͆B. hampsonii ”ࡢྠᐃ᪉ἲࡋ࡚ࠊnox 㑇ఏᏊ㓄ิࡢ⣔⤫ᶞゎᯒࠊ16 S rRNA 㑇ఏᏊゎᯒࠊ“B. hampsonii ”≉␗ⓗ࡞ 23S rRNA sequence ࣉࣟ ࣮ࣈࢆ⏝࠸ࡓ Fluorescent in situ hybridization [5]ࡀሗ࿌ࡉࢀ࡚࠸ࡿࠋࡇ ࡢࣉ࣮ࣟࣈࡣ“B. hampsonii ”ࡢ clade I clade ϩࢆ᳨ฟࡍࡿࡇࡀ࡛ࡁ ࡿࡶࡢࡢ B. intermedia ࡶᛂࡍࡿࡇࡽὀពࡀᚲせ࡛࠶ࡿ[5]ࠋ ࠙㇜㉥⑩㢮ఝࠚ ㇜㉥⑩㢮ఝࡋࡓ≧ࢆ♧ࡍࡋ࡚ࠊ㇜⤖⭠ࢫࣆ࣮ࣟ࣊ࢱ[31]ࠊ ㇜ࢧࣝࣔࢿࣛ[29]ࠊ㇜ቯṚᛶ⭠⅖ࠊቑṪᛶ⭠⅖ࠊ⫶₽⒆ࠊⅣࠊఏᰁᛶ ⫶⭠⅖ࠊ㇜࣓࣮ࣂ[27㸪43]࡞ࡀ࠶ࡿࠋ࡞࡛ࡶ㇜࣓࣮ࣂ㇜㉥ ⑩ࡣᒕ␆ሙࡢ⫗║᳨ᰝ࡛༊ูࡍࡿࡇࡣ㞴ࡋࡃࠊ᳨ᰝᐊෆ࡛ࡢ᳨ᰝࡀᚲせ ࡞ࡿࠋࡲࡓࠊ୧⪅ࡀΰྜឤᰁࡍࡿࡇࡶ࠶ࡿ[43]ࠋ ࠙ᢠ⏕≀㉁ࡢ⏝つไࡼࡿᙳ㡪ࠚ ᪥ᮏ࡛ࡣື≀⏝་⸆ရࡢ⏝つไ┬௧ࡢᨵṇ➼ࡀ 2013 ᖺ 5 ᭶ 30 ᪥ බᕸࡉࢀࠊ2013 ᖺ 11 ᭶ 30 ᪥௨㝆ࠊつไᑐ㇟࡞ࡿ 13 ≀㉁ࡀ⏝⚗Ṇ ࡞ࡗࡓࠋ㣗⏝ື≀ື≀⏝་⸆ရࡀṧ␃ࡍࡿࡇࡼࡿேࡢᗣࡢᙳ㡪 ࢆ㜵ࡄࡓࡵ࡛࠶ࡿࡶࡢࡢࠊ㇜ࢆྵࡴ㣗⏝ື≀⣽⳦➼ࡢឤᰁࡀቑຍࡍࡿ ྍ⬟ᛶࡀ༴ࡉࢀࡿࠋ ࠙⤖ㄒ 㹼ᚋࡢㄪᰝ࣭◊✲ࡢ᪉ྥᛶ㹼ࠚ ࡇࢀࡲ࡛ࠊ᪥ᮏࢆྵࡵࡓࢪ࡛ࡣ“B. suanatina”͆B. hampsonii ” ࡢᏑᅾࡣ☜ㄆࡉࢀ࡚࠸࡞࠸ࠋࡋࡋࠊ⡿ཬࡧ࣮ࣚࣟࢵࣃ㸦࢝ࢼࢲࠊ࣓ ࣜ࢝ཬࡧࢹ࣐࣮ࣥࢡ➼㸧ࡽᖺ㛫⣙ 500㹼1000 㢌ࡢ⏕యࢆ㍺ධࡋ࡚࠸ࡿ (ື≀᳨ᖺሗ 2009 ᖺ㹼2012 ᖺ)ࡇࢆ⪃៖ࡍࡿࠊ᪤᪥ᮏࡢ㇜ࡸ㔝㫽 ➼ឤᰁࡋ࡚࠸ࡿྍ⬟ᛶࡶ࠶ࡿࠋ ᪥ᮏࡢྛ✀㔝⏕ື≀࠾ࡅࡿࠊ“B. suanatina”“B. hampsonii ”ࢆྵࡵ ࡓࣈࣛ࢟ࢫࣆࣛᒓ⳦ࡢศᕸࠊಖ⳦⋡ࠊከᵝᛶࠊᢠ⳦⸆ឤཷᛶ➼ࡘ࠸࡚ࡣࠊ ౫↛᫂࡞Ⅼࡶከ࠸ࡇࡽࠊᚋࡢヲ⣽࡞ㄪᰝࡀᚲせ࡛࠶ࡿࠋ Shibahara T, et al.: 29 Brachyspira 2015:5(1):30 ᚋࡣ㇜࠾ࡅࡿ㇜㉥⑩ᵝ㛵ࡍࡿ▱ぢࢆ㞟✚ࡍࡿࡶࠊࡇࢀ ࡽ᪂ࡓ࡞ Brachyspira ࢆྵࡵࡓ᳨ᰝ࣭ྠᐃࡀᚲせ⪃࠼ࡽࢀࡿࠋࡲࡓࠊࡇ ࢀࡲ࡛ B. hyodysenteriae ࡼࡿ㇜㉥⑩デ᩿ࡉࢀ࡚࠸ࡓࡢ࠺ࡕࠊ“B. suanatina”࠶ࡿ࠸ࡣ͆B. hampsonii ”ࡼࡿ⢓⾑ᛶୗ⑩ࡀྵࡲࢀ࡚࠸ࡿྍ ⬟ᛶࡶ࠶ࡿࡇࡽࠊศ㞳ಖᏑᰴ୪ࡧࣃࣛࣇࣥໟᇙࣈࣟࢵࢡ➼ࢆ⏝࠸ ࡓᅇ㢳ㄪᰝࡼࡾࠊ᪥ᮏ࠾ࡅࡿ“B. suanatina ”͆B. hampsonii ”ࡢᾐ ₶≧ἣࡸࡑࡢ⣽⳦Ꮫⓗ≉ᚩࠊឤᰁ※ࠊឤᰁ⤒㊰ࠊ⏕యࡢᙳ㡪➼ࡘ࠸࡚ ᫂ࡽࡋ࡚࠸ࡃᚲせࡀ࠶ࡿࠋ ✏ࢆ⤊࠼ࡿ࠶ࡓࡾࠊᣦᑟࠊຓゝࢆ࠸ࡓࡔ࠸ࡓࠊⲈᇛᏛ ㊊❧ྜྷᩝᩍ ᤵࠊᾏ㐨▼⊁ᐙ␆ಖ⾨⏕ᡤ ᒣᮏᩔᏊ⋇་ᖌࠊ༓ⴥ┴୰ኸᐙ␆ಖ⾨ ⏕ᡤ 㛵ཱྀ┿ᶞ⋇་ᖌࠊື≀⾨⏕◊✲ᡤ ᑠᯘᢏᖌཬࡧᔱ⏣ᜨ⨾ᢏᖌ ῝ㅰࡍࡿࠋ ͤ 㐃⤡㈐௵⪅:Ⱚཎᖾ ࠛ305-0856 Ⲉᇛ┴ࡘࡃࡤᕷほ㡢ྎ 3-1-5 (⊂) ㎰ᴗ࣭㣗ရ⏘ᴗᢏ⾡⥲ྜ◊ ✲ᶵᵓ ື≀⾨⏕◊✲ᡤ ែ◊✲㡿ᇦ ; tel: 029-838-7774㸹 fax: 029-838-7774; e-mail address: [email protected] (ㄽᩥཷ㸸㸰㸮㸯㸲ᖺ㸷᭶㸰᪥ࠊཷ⌮㸸2014 ᖺ 11 ᭶ 13 ᪥) ᘬ⏝ᩥ⊩ 1. 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Shibahara T, et al.: 37 Brachyspira 2015:5(1):38 Education lecture Brachyspira and swine dysentery Authors: Tomoyuki Shibahara 1ͤ, Hanae Inagaki1,2 Institution: 1Pathology and Pathophysiology Research Division, National Institute of Animal Health, National Agricultural and Food Research Organization, 2Ishikari Livestock Hygiene Service Center, Japan Summary Brachyspira hyodysenteriae and Brachyspira pilosicoli are associated with swine dysentery and porcine colonic spirochetosis in pigs. Molecular (the nox and 16S ribosomal RNA genes) and biochemical analysis of atypical strongly beta-hemolytic isolates suggests that a novel species has emerged in the United States, Canada and European countries with the proposed name “Brachyspira hampsonii ”. This species is divided into two clades, “B. hampsonii ” clade I and clade ϩ. It causes clinical disease similar to that caused by B. hyodysenteriae. The objective of this review is to summarize the current knowledge on the pathogenicity and prevalence of “B. hampsonii ”. Key words: ͆Brachyspira hampsonii ”, Brachyspira hyodysenteriae, swine dysentery ͤ Corresponding author: Tomoyuki Shibahara, Pathology and Pathophysiology Research Division, National Institute of Animal Health, 3-1-5 Kannondai, Tsukuba, Ibaraki 305-0856, Japan TEL: +81-29-838-7774㸹FAX: +81-29-838-7880 E-mail address: [email protected] (This paper was received on September 2, 2014, and accepted on November 13, 2014.) Brachyspira 2015:5(1):39 Brachyspira 2015:5(1):39 Brachyspira 2015;5(1): 38 6 ᅇ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛᏛ⾡㞟ሗ࿌ ➨➨ 6 ᅇ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛᏛ⾡㞟ሗ࿌ th meeting of the Japanese Society of Brachyspira Report of the Japanese Society of Brachyspira Report on on thethe 6th6meeting ᮾி་⛉ᏛⲈᇛ་⒪ࢭࣥࢱ࣮ ᒾᮏ῟୍ ᮾி་⛉ᏛⲈᇛ་⒪ࢭࣥࢱ࣮ ᒾᮏ῟୍ JyunichiIwamoto Iwamoto Jyunichi Ibaraki Medical center Ibaraki Medical center Toukyou 㸦ᮾி་⛉ᏛⲈᇛ་⒪ࢭࣥࢱ࣮㸧 University 㸦ᮾி་⛉ᏛⲈᇛ་⒪ࢭࣥࢱ࣮㸧 Toukyou medical medical University 6 ᅇ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛᏛ⾡㞟ࡀᖹᡂ 7᭶ ᪥Ⲉᇛ┴ࡘ ➨➨ 6 ᅇ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛᏛ⾡㞟ࡀᖹᡂ 26 26 ᖺᖺ 7᭶ 26 26 ᪥Ⲉᇛ┴ࡘ ࡃࡤᕷࡢࡘࡃࡤᅜ㝿㆟ሙ࡚㛤ദࡉࢀࡓࠋᏛ⾡㞟࡛Ⓨ⾲ࡉࢀࡓ᭱᪂ࡢ ࡃࡤᕷࡢࡘࡃࡤᅜ㝿㆟ሙ࡚㛤ദࡉࢀࡓࠋᏛ⾡㞟࡛Ⓨ⾲ࡉࢀࡓ᭱᪂ࡢ ヰ㢟㛵ࡋ࡚ሗ࿌ࡍࡿࠋᙜᏛ⾡㞟㛤ദ࠶ࡓࡾࠊࡢ⣲〇⸆ࠊࢫࢸࣛ ヰ㢟㛵ࡋ࡚ሗ࿌ࡍࡿࠋᙜᏛ⾡㞟㛤ദ࠶ࡓࡾࠊࡢ⣲〇⸆ࠊࢫࢸࣛ ࢫ〇⸆ࠊࢫࢺࣛࢮࢿ࢝ࠊࢵࣦྜྠࠊ࣎ࢵࢺࢪࣕࣃࣥࠊ࢚࣮ࢨࠊ ࢫ〇⸆ࠊࢫࢺࣛࢮࢿ࢝ࠊࢵࣦྜྠࠊ࣎ࢵࢺࢪࣕࣃࣥࠊ࢚࣮ࢨࠊ ሯ〇⸆ࠊᑠ㔝⸆ရᕤᴗࠊሯ〇⸆ᕤሙࠊࢶ࣒ࣛࠊᮥᯘ〇⸆ࠊ༠Ⓨ㓝࢟ ሯ〇⸆ࠊᑠ㔝⸆ရᕤᴗࠊሯ〇⸆ᕤሙࠊࢶ࣒ࣛࠊᮥᯘ〇⸆ࠊ༠Ⓨ㓝࢟ ࣜࣥࠊṇᐩᒣ་⸆ရࠊ᪥ᮏఫ〇⸆ࠊṊ⏣〇⸆ᕤᴗࠊ⏣㎶୕⳻〇⸆ࠊ ࣜࣥࠊṇᐩᒣ་⸆ရࠊ᪥ᮏఫ〇⸆ࠊṊ⏣〇⸆ᕤᴗࠊ⏣㎶୕⳻〇⸆ࠊ ᐑධࡉࢇ〇⸆ࡢྛᰴᘧ♫ࡢ༠㈶ࢆ࠸ࡓࡔࡁࡲࡋࡓࠋᏛ⾡㞟ཬࡧ⥲ࡢ ᐑධࡉࢇ〇⸆ࡢྛᰴᘧ♫ࡢ༠㈶ࢆ࠸ࡓࡔࡁࡲࡋࡓࠋᏛ⾡㞟ཬࡧ⥲ࡢ ᴫᐜࡣୗグࡢ࠾ࡾ࡛ࡍࠋ ᴫᐜࡣୗグࡢ࠾ࡾ࡛ࡍࠋ Meeting Japanese Society Brachyspira was held The Meeting of of Japanese Society of of Brachyspira was held in in The 6th6th Tsukubainternational internationalconvention conventioncenter centerand andthethemeeting meetingwas was Tsukuba organized Tokyo Medical University July 2014. was organized by by Tokyo Medical University on on July 26,26, 2014. It It was supported Ajnomoto pham., ltd., -------. The outlineof of supported by by Ajnomoto pham., co.co. ltd., -------. The outline thethe Meeting was follows: Meeting was as as follows: ≉ูㅮ₇࣭ᩍ⫱ㅮ₇ ϨϨ ≉ูㅮ₇࣭ᩍ⫱ㅮ₇ ≉ูㅮ₇ I ࡛ࡣࠊ㜵⾨་⛉Ꮫ⮫ᗋ᳨ᰝ་Ꮫࡢ⥴᪉⾪ඛ⏕ࡣࠕࣄࢺ⭠⟶ ≉ูㅮ₇ I ࡛ࡣࠊ㜵⾨་⛉Ꮫ⮫ᗋ᳨ᰝ་Ꮫࡢ⥴᪉⾪ඛ⏕ࡣࠕࣄࢺ⭠⟶ ࢫࣆ࣮ࣟ࣊ࢱࡢ⮫ᗋ⌮Ꮫⓗ≉ᚩࡘ࠸࡚࡛ࠖㅮ₇ࡉࢀࡓࠋෆᐜࡋ࡚ ࢫࣆ࣮ࣟ࣊ࢱࡢ⮫ᗋ⌮Ꮫⓗ≉ᚩࡘ࠸࡚࡛ࠖㅮ₇ࡉࢀࡓࠋෆᐜࡋ࡚ ࡣࠊ ⭠ෆど㙾ୗ࡛᥇ྲྀࡉࢀࡓ᳨యࡘ࠸࡚ࠊ HIS ≉ᚩⓗ࡞ fringe ᙧᡂ ࡣࠊ ⭠ෆど㙾ୗ࡛᥇ྲྀࡉࢀࡓ᳨యࡘ࠸࡚ࠊ HIS ≉ᚩⓗ࡞ fringe ᙧᡂ ࡢデ᩿᪉ἲࡘ࠸࡚ࠊ ࡉࡽ⮬⾨㝲㝔࡛ࡢከࡃࡢࡢ᳨ウ࡛ HIS ឤᰁ ࡢデ᩿᪉ἲࡘ࠸࡚ࠊ ࡉࡽ⮬⾨㝲㝔࡛ࡢከࡃࡢࡢ᳨ウ࡛ HIS ឤᰁ ࡢෆど㙾ീࡢ≉ᚩࡘ࠸࡚Ⓨ⾲ࡉࢀࡓࠋHIS ឤᰁ≉ᚩⓗ࡞ᡤぢࡋ ࡢෆど㙾ീࡢ≉ᚩࡘ࠸࡚Ⓨ⾲ࡉࢀࡓࠋHIS ឤᰁ≉ᚩⓗ࡞ᡤぢࡋ Brachyspira 2015;5(1): 39 Brachyspira 2015:5(1):40 ࡚⾑⟶㏱ぢീࢆኻࡗࡓ⢒㐀⢓⭷ࠊ⢓⭷Ⓨ㉥࣭⾑ࠊ㗬ṑ≧࣏࣮ࣜࣉࠊ⢓ㄪ ࡞⢓ᾮ㸧ࡀ࠶ࡾࡑࢀࡽࡀྑഃ⤖⭠ࢆ୰ᚰㄆࡵࡓࡍࡿሗ࿌࡛࠶ࡗࡓࠋ ≉ูㅮ₇ II ࡣ Helicobacter pylori ឤᰁ㛵ࡍࡿ◊✲࡛ࡣᾏ㐨Ꮫ㝃 ᒓ㝔ගᏛ་⒪デ⒪㒊ࡢ㛫㒊ඞ⿱ඛ⏕ࡽࠊࠕࣄࢺ࠾ࡅࡿࢫࣆ࣮ࣟ࣊ࢱ ឤᰁࡣኚࢆక࠺㸽SSA/P FGIDsࠖࡢㅮ₇ࢆࡉࢀࡓࠋHelicobacter pylori ឤᰁ HIS ឤᰁẚ㍑ࡋ࡞ࡀࡽゎࡾࡸࡍࡃゎㄝࡉࢀࡓࠋࡲࡓ⭠ ⭘⒆ᛶኚࠊSessile serrated adenoma/ polyp (SSA/P)࡛ࡣࢥࣥࢺ࣮ࣟࣝ ࡢ⭢⭘ࡢẚ㍑࡛ேࢫࣆ࣮ࣟ࣊ࢱឤᰁ㸦HIS㸧ࡀ㧗⋡ㄆࡵࡽࢀࠊSSA/P HIS ࡢᙉ࠸┦㛵㛵ಀᛶࢆ♧၀ࡍࡿኚ⯆῝࠸ࡶࡢ࡛࠶ࡗࡓࠋ ⲈᇛᏛࡢ㊊❧ྜྷᩝඛ⏕ࡢᩍ⫱ㅮ₇࡛ࡣࠊࠕࣈࣛ࢟ࢫࣆࣛᒓ⳦ࡢᙧែኚ ␗ࡘ࠸࡚ࠖࡢ◊✲⤖ᯝࡘ࠸࡚ㅮ₇ࡉࢀࡓࠋࣈࣛ࢟ࢫࣆࣛᒓ⳦ࡣ୍⯡ ⼺᪕≧࡛࠶ࡿࡀࠊ㢖ᅇ⥅௦ࢆ⧞ࡾ㏉ࡍࡇࡼࡗ࡚ࠊ⼺᪕≧ࡽᱰ≧ኚ ࡍࡿࡢ⤖ᯝࡀ B.pilosicoliATCC51139 ࡛ㄆࡵࡽࢀࡓࠋ୍᪉㇜㉥⑩ࡢ ཎᅉ⳦ B.hyodysenteriae ࡣ⥅௦ࢆ⧞ࡾ㏉ࡋ࡚ࡶ⼺᪕≧ࡣኚࡏࡎ㑇ఏⓗ Ᏻᐃࡋࡓᙧែ࡛࠶ࡗࡓࡀࠊ⮬↛⏺ࡣ B.hyodysenteriae ࡢᱰ≧ኚ␗ᰴ ࡀᏑᅾࡋࠊࣈࣛ࢟ࢫࣆࣛᒓ⳦ࡢከࡃࡣᙧែኚ␗ࢆ࠾ࡇࡍࡇࡀド᫂ࡉࢀࡓࠋ II ୍⯡₇㢟 ᅇࡣィ 11 㢟ࡢ୍⯡₇㢟ࡀⓎ⾲ࡉࢀࡓࠋ᪥ᖖデ⒪࡛ HIS デ᩿ࡉࢀࡓ ከࡃࡢࡀ࿊♧ࡉࢀࡓࠋ᪥ᖖデ⒪࡛ࡢ⌮⤌⧊デ᩿࠾࠸࡚ HIS ឤᰁࢆ デ᩿ࡉࢀࡿᶵࡀቑ࠼࡚ࡁ࡚࠸ࡿࡇࡶሗ࿌ࡉࢀࠊᚋࡶ⭠ෆど㙾᳨ᰝ ࡚ HIS ࡀ⌮Ꮫⓗデ᩿ࡉࢀࡿࡢቑຍࡀணࡉࢀࡿࠋ ࡲࡓࠊୗ⑩➼ࡢᾘჾ≧ࡀㄆࡵࡽࢀ HIS デ᩿ࡉࢀࡓࡢ࣓ࢺࣟ ࢽࢲࢰ࣮ࣝࡼࡿ⒪࡛ࠊࡑࡢ᭷⏝ᛶࡀሗ࿌ࡉࢀࡓࠋࡲࡓ≧ࡀㄆࡵࡽࢀ ࡞࠸ሙྜࡢ⒪ࡢᚲせᛶ㛵ࡋ࡚ࡣ୍ᐃࡢぢゎࡀᚓࡽࢀ࡚࠸࡞࠸༳㇟࡛ ࠶ࡗࡓࡢ࡛ᚋࡢ᳨ウㄢ㢟ᛮࢃࢀࡓࠋ ࣓࣮ࣂᛶ⭠⅖ࡸ₽⒆ᛶ⭠⅖➼ࡢ⭠ᝈ HIS ឤᰁࡀྜేࡍࡿ ࡢሗ࿌ࡶ࡞ࡉࢀࠊࡑࢀࡽࡢᝈ࡛ࡢ HIS ྜేឤᰁὀពࢆせࡍࡿࡇࡀ ♧၀ࡉࢀࡓࠋࡲࡓ࣓࣮ࣂ⭠⅖ࡸ₽⒆ᛶ⭠⅖➼ྜేࡍࡿ HIS ឤᰁ࠾ Brachyspira 2015;5(1): 40 Brachyspira 2015:5(1):41 ࠸࡚ࡶ࣓ࢺࣟࢽࢲࢰ࣮ࣝࡢ᭷ຠᛶࡀሗ࿌ࡉࢀࡓࠋ ⲈᇛᏛࡢሗ࿌࡛ࠊHIS ᳨ᰝࡢ౫㢗ࢆ࠺ࡅࡓ㝔࡛ࡢ HIS ឤᰁ ࡽࡢࠊAcoliF1/R583 ࠸ࡗࡓ✀デ᩿⏝ࣉ࣐࣮࡛ࣛࡣቑᖜࡉࢀࡎࠊ F3/R500 ࠸࠺ࣈࣛ࢟ࢫࣆࣛᒓ⳦ࡢඹ㏻ࣉ࣐࣮࡛ࣛࡣቑᖜࡉࢀࠊࡑࡢሷ ᇶ 㓄 ิ ୰ -TTTTTT- ࡢ 㓄 ิ ࡀ ☜ ㄆ ࡉ ࢀ ࡓ ࡇ ࢁ ࡽ Brachyspira pilosicoli デ᩿ࡋࡓ⤒㦂ࡀⓎ⾲ࡉࢀࡓࠋࡉࡽⲈᇛᏛࡢⓎ⾲࡛ࠊឡ▱┴ ⩌㤿┴࡛㇜ࡽศ㞳ࡉࢀࡓ Brachyspira ᒓ⳦ࡢ࣐ࢡࣟࣛࢻ⪏ᛶࡘ࠸ ࡚ሗ࿌ࡉࢀࡓࠋ࣐ࢡࣟࣛࢻ⣔ᢠ⏕ࡣ㇜ࡢ྾ჾᝈࡢ⒪⏝࠸ࡽࢀ ࡿᢠ⏕࡛࠶ࡿࡀࠊᚋ⪏ᛶ⋓ᚓࡍࡿ⳦ᰴࡀቑຍࡍࡿࡇࡼࡿ༴㝤ᛶࡀ ♧ࡉࢀࡓࠋሷᇶ㓄ิ┦ྠ⋡ᇶࡃุูᘧࡀሗ࿌ࡉࢀࠊᚋࢥࣥࣆ࣮ࣗࢱ࣮ ୖ࡛ࡇࡢุูᘧ࡛ィ⟬࡛ࡁࡿࡼ࠺࡞ࢀࡤ㇜㉥⑩ࡢุูࡣ᭦⡆᫆࡞ ࡿࡇࡀ♧ࡉࢀࡓࠋ III Ꮫ㈹ (᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ㈹/᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛዡບ㈹) ➨㸴ᅇࡢᏛ⾡㞟࡛ࠊ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ㈹᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ ዡບ㈹ࡢ⾲ᙲࡀ⾜ࢃࢀࡓࠋ ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ㈹ࡣࠊHIS ࡢឤᰁࡢ⌮Ꮫⓗデ᩿࠾࠸࡚ᮏឤᰁ ࡢ◊✲ࡁࡃ㈉⊩ࡉࢀࡓ㜵⾨་⛉Ꮫ⮫ᗋ᳨ᰝ་Ꮫࡢ⥴᪉⾪ඛ⏕ Ỵᐃࡉࢀࡓࠋ⥴᪉ඛ⏕ࡢ◊✲࡛ࡣࠊ⮬⾨㝲࡛ࡢከࡃࡢ HIS ឤᰁࡀ⮫ᗋ⌮ Ꮫⓗ᳨ฟࡉࢀ࡚࠾ࡾࡑࡢ⮫ᗋീࡘ࠸࡚ከࡃࡢ⤖ᯝࢆሗ࿌ࡉࢀ࡚࠸ࡿࠋ ࡲࡓ⥴᪉ඛ⏕ࡣ⮬⾨㝲㝔ࡢࡳ࡞ࡽࡎᡤἑᕷ୰㝔ࡢ⮫ᗋ⌮᳨య࡛ࡶ HIS ឤᰁࡀᑡ࡞ࡽࡎᏑᅾࡍࡿࡇࢆሗ࿌ࡉࢀ࡚࠸ࡿࠋH.E.ᰁⰍࡢ ⌮᳨య࡛ HIS デ᩿ࡀྍ⬟࡛࠶ࡿࡇࢆከࡃࡢࢹ࣮ࢱࢆࡲࡵ࡚ሗ࿌ࡋ ࡚࠸ࡿࠋࡑࡢከࡃࡢ◊✲ᡂᯝࡣ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛࡢ㈉⊩ࡀࡁࡃཷ ㈹࡞ࡗࡓࠋ ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛዡບ㈹ࡣ⥴᪉ඛ⏕ඹྠ◊✲࡛ከࡃࡢ HIS ឤᰁ ࡢ⭠ෆど㙾ീࢆ᫂ࡽࡋࡓ⮬⾨㝲࿋㝔ࡢᮾᒣṇ᫂ඛ⏕Ỵࡲࡗࡓࠋ ᮾᒣṇ᫂ඛ⏕ࡢ◊✲࡛ࡣࠊ⮬⾨㝲㝔࡛ࡢከࡃࡢ HIS ឤᰁࡢࡢෆど㙾 ീࢆ᳨ウࡉࢀࡑࡢ≉ᚩࡘ࠸࡚ሗ࿌ࡉࢀ࡚࠸ࡿࠋࡇࢀࡲ࡛ HIS ឤᰁࡢ⭠ Brachyspira 2015;5(1): 41 Brachyspira 2015:5(1):42 ෆど㙾ᡤぢࡘ࠸࡚ࡲࡲࡗࡓࢹ࣮ࢱࡣ࡞ࡃࠊᚋᮾᒣඛ⏕ࡢ◊✲ᡂᯝࡀ ⮫ᗋ⏕ࡉࢀࠊ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛࡁࡃ㈉⊩ࡉࢀࡿࡇࡀᮇᚅࡉ ࢀ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛዡບ㈹ࡀỴᐃࡉࢀࡓࠋࡇࢀࡽࡢ㈹ࡣ᪥ᮏࣈࣛ࢟ࢫ ࣆࣛᏛ㛗ࡢ㊊❧ྜྷᩝඛ⏕ࡽ⥴᪉⾪ඛ⏕ᮾᒣṇ᫂ඛ⏕㈹ࡶ ㉗ࡽࢀࡓࠋ ࠾ࢃࡾ ᮶ᖺࡢ➨ 7 ᅇᏛ⾡㞟ࡣಙᕞᏛࡢྜྷ⃝᫂ᙪඛ⏕㸦ி㒔Ꮫ㌿ฟࡉࢀࠊ ➨㸶ᅇࡢᏛ⾡㞟ࡣி㒔࡛㛤ദࡉࢀࡿணᐃ㸧ࡀୡヰே࡞ࡾ⾜ࢃࢀࡿணᐃ ࡛࠶ࡿࠋࡇࡢᏛ⾡㞟࡛ࡣᏛဨࠊ㠀Ꮫဨࡽᗈࡃ₇㢟ࢆເ㞟ࡍࡿணᐃ ࡛࠶ࡿࡢ࡛᪥ᖖデ⒪࡛ HIS デ᩿ࡉࢀࡓࢆ࠾ᣢࡕࡢඛ⏕᪉ࡣ㠀ࡈ Ⓨ⾲࠸ࡓࡔࡁࡓ࠸ࠋ Brachyspira 2015;5(1): 42 Brachyspira 2015:5(1):43 ➨㸴ᅇ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛᏛ⾡㞟ࣉࣟࢢ࣒ࣛ 9 30 ศ 㛤ሙ ཷ㛤ጞ 10 00 ศ 㛤ᣵᣜ ᒾᮏ῟୍ 㸦ᮾி་⛉Ꮫ㸧 10 05 ศ㹼10 40 ศ ≉ูㅮ₇ I ᗙ㛗 ᒾᮏ῟୍㸦ᮾி་⛉ Ꮫ㸧 ࣄࢺ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱࡢ⮫ᗋ⌮Ꮫⓗ≉ᚩࡘ࠸࡚ ㅮᖌ㸸 ⥴᪉ ⾪ 㸦㜵⾨་⛉Ꮫᰯ⮫ᗋ᳨ᰝ་Ꮫㅮᗙ㸧 10 40 ศ㹼11 40 ศ ୍⯡₇㢟 ᗙ㛗 ᯘ ಇ 㸦㔛 Ꮫ㸧 1. ↓≧࡚Ⓨぢࡉࢀࡓ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱࡢ୍ 㛫ᐑ Ꮥ 㸦ࡦࡓࡕ࡞⥲ྜ㝔ࠊ⟃ἼᏛᒓ㝔ࡦࡓࡕ࡞ ♫ᩍ⫱◊✲ࢭࣥࢱ࣮㸧 2. metronidazole㸦1500mg/day㸧ᢞ࡚⒵ࡋࡓୗ⑩య㔜ῶᑡࢆ కࡗࡓ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱࡢ 1 ̿ᮏ㑥ሗ࿌ 26 ࡢ᳨ウ̿ ᐑᕝ᫂♸ 㸦ᅜಖ᪫୰ኸ㝔 ᾘჾෆ⛉㸧 3. ᪥ᖖ⮫ᗋ࡛⤒㦂ࡋࡓ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱ 5 ࡢ᳨ウ ᖹ▼ဴஓ 㸦ᕝᓮᕷ❧ከᦶ㝔 ᾘჾ⫢⮚ෆ⛉㸧 4. ᮍᡂᖺ⪅࠾ࡅࡿࣄࢺ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱ(HIS)ࡢ᳨ウ ୍ⴙ⏣ṇ㑻 㸦ಙᕞᏛ་Ꮫ㒊㝃ᒓ㝔⮫ᗋ᳨ᰝ㒊㸧 11 40 ศ 㹼 13 00 ศ 㣗 ࣭ ఇ ᠁ 㸦 ᖿ 㸧 13 00 ศ㹼13 15 ศ ⥲ 13 15 ศ㹼14 00 ศ ≉ูㅮ₇ II ᗙ㛗 ⥴᪉⾪㸦㜵⾨་⛉Ꮫᰯ㸧 ࣄࢺ࠾ࡅࡿࢫࣆ࣮ࣟ࣊ࢱឤᰁࡣཎᛶࢆక࠺㸽 66$3 )*,'V 㛫㒊ඞ⿱ 㸦ᾏ㐨Ꮫ㝔 ගᏛ་⒪デ⒪㒊㸧 14 00 ศ㹼14 40 ศ ᩍ⫱ㅮ₇ ᗙ㛗 ᮎྜྷ┈㞝㸦ᐑᓮᏛ㸧 ࣈࣛ࢟ࢫࣆࣛᒓ⳦ࡢᙧែኚ␗ࡘ࠸࡚ ㊊❧ྜྷᩝ 㸦ⲈᇛᏛ㎰Ꮫ㒊㸧 14 40 ศ㹼14 50 ศ ఇ᠁ 14 50 ศ㹼15 20 ศ ୍⯡₇㢟 ᗙ㛗 ➉⃝ᩗே㸦⮬་⛉Ꮫ㸧 Brachyspira 2015;5(1): 43 Brachyspira 2015:5(1):44 5. Acoli1/R583 ࣉ࣐࣮࡛ࣛࡣᛂࡋ࡞࠸ Brachyspira pilosicoli ࡢᝈ ⪅ࡽࡢศ㞳 ㊊❧ྜྷᩝࠊᚋ⸨⪔ྖ 㸦ⲈᇛᏛ㎰Ꮫ㒊㸧(ᮾிᏛ㝔) 6. ᮾศ㞳ᰴ Brachyspira pilosicoli ࡢᙧែ ㊊❧ྜྷᩝࠊᚋ⸨⪔ྖ 㸦ⲈᇛᏛ㎰Ꮫ㒊㸧(ᮾிᏛ㝔) 15 20 ศ㹼16 05 ศ ୍⯡₇㢟 ᗙ㛗 ྜྷ⃝᫂ᙪ㸦ಙᕞᏛ㸧 7. ᙜ㝔࡛⤒㦂ࡋࡓ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱྜేࡋࡓ࣓࣮ࣂ⭠⅖ࡢ 2 ➉⃝ᩗே 㸦⮬་⛉Ꮫෆ⛉Ꮫㅮᗙᾘჾෆ⛉㒊㛛㸧 8. ⅖ᛶ⭠ᝈ࠾ࡅࡿࣄࢺ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱឤᰁྜేࡘ࠸࡚ࡢ᳨ウ ᒾᮏ ῟୍ 㸦ᮾி་⛉ᏛⲈᇛ་⒪ࢭࣥࢱ࣮ᾘჾෆ⛉㸧 9. ᮾி㏆㑹ࡢ᳨ᰝࢭࣥࢱ࣮ཷク᳨య࠾ࡅࡿࣄࢺ⭠⟶ࢫࣆ࣮ࣟ࣊ࢱ ࡢ⮫ᗋ⌮Ꮫⓗ᳨ウ ⥴᪉ ⾪ 㸦㜵⾨་⛉Ꮫᰯ⮫ᗋ᳨ᰝ་Ꮫㅮᗙ㸧 16 05 ศ㹼16 35 ศ ୍⯡₇㢟 ᗙ㛗 ୰ᓥᘯ⨾㸦ⲈᇛᏛ㸧 10. ឡ▱┴࠾ࡼࡧ⩌㤿┴࡛ศ㞳ࡉࢀࡓ Brachyspira ᒓ⳦ࡢ࣐ࢡࣟࣛ ࢻ⪏ᛶ ᑠ⃝⩫ 㸦ⲈᇛᏛ㎰Ꮫ㒊㸧 11. ㇜ࡽศ㞳ࡉࢀࡓࣈࣛ࢟ࢫࣆࣛᒓ⳦୰ Brachyspira hyodysenteriae ࡢ ሷᇶ㓄ิ┦ྠᛶ㛵ࢃࡿุูᘧࡼࡿྠᐃ ᑠ⃝⩫ 㸦ⲈᇛᏛ㎰Ꮫ㒊㸧 16 35 ศ 㛢ᣵᣜ ᒾᮏ῟୍ 㸦ᮾி་⛉Ꮫ㸧 17 30 ศ㹼19 30 ศ ᠓ぶ Brachyspira 2015;5(1): 44 Brachyspira 2015:5(1):45 ࠕBrachyspiraࠖᢞ✏つᐃ 㸯㸬 ࠕBrachyspiraࠖࡣࠊࣈࣛ࢟ࢫࣆࣛ㛵ࡍࡿ◊✲ࡢᡂᯝࢆබ⾲ࡍࡿࡇ ࢆ┠ⓗࠊ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛࡀᖺ 1 ᅇⓎ⾜ࡍࡿࠋ 㸰㸬ᥖ㍕ㄽᩥࡣࠊཎⴭࠊ⥲ㄝࠊᏛ࠾࠸࡚Ⓨ⾲ࡉࢀࡓ≉ูㅮ₇ࠊࢩ࣏ࣥ ࢪ࣒࢘➼ࡢせ᪨ཬࡧࣈࣛ࢟ࢫࣆࣛ᭷┈࡞ᢏ⾡ࡢሗ㸦ᢏ⾡ሗ࿌ู࣭㡯 ཧ↷㸧ࡍࡿࠋ 㸱㸬ㄽᩥࡢⴭ⪅ࡣᮏᏛࡢဨ࡛࠶ࡿࡇࡀᮃࡲࡋ࠸ࡀࠊᢞ✏ࡢせ௳ࡣ ࡋ࡞࠸ࠋㄽᩥࡢⴭ⪅ࡣࠊᢞ✏ඛ❧ࡕᮏᏛࡢᢞ✏つᐃࠊ᭱᪂ࡢㄅ➼ ࢆཧ⪃ࡋ࡚ࠊཎ✏ࢆసᡂࡍࡿࠋ 㸲㸬ㄽᩥࡢ᥇ྰࡣ⦅㞟ጤဨࡀỴᐃࡍࡿࠋࡇࡢࡓࡵ⦅㞟ጤဨࡣ㐺௵⪅ ᰝㄞࢆ౫㢗ࡋࠊཷ⌮ඛ❧ࡕⴭ⪅ࡑࡢෆᐜ➼ࡢኚ᭦ࢆồࡵࡿࡇࡀ࠶ ࡿࠋ 㸳㸬ཎ✏ࡣⱥᩥࡶࡋࡃࡣᩥ࡛సᡂࡋࠊ3 㒊㸦ෆ 2 㒊ࡣ᫂░࡞ࢥࣆ࣮࡛ࡶ Ⰻ࠸㸧ࢆ⦅㞟ᖿ㸦㈐௵⪅㸧᭩␃㒑౽࡛ᥦฟࡍࡿࠋᢞ✏࠶ࡓࡾࠊㄽ ᩥᥖ㍕ࡲ࡛ࡢᑐᛂࢆ⾜࠺௦⾲⪅ࡣࠊᢞ✏ཎ✏ࡀࠊㄅࡍ࡛ᥖ㍕࠶ࡿ ࠸ࡣᢞ✏୰ࡶࡋࡃࡣᢞ✏ணᐃ࡛ࡣ࡞࠸ࡇࡍࡿࠋ 㸴㸬ㄽᩥࡢ༳ๅ㈝ࠊูๅ௦ࡣⴭ⪅ࡢ㈇ᢸࡍࡿࠋࡓࡔࡋࠊཎⴭࠊせ᪨࠾ ࠸࡚ࡣཎ๎ࡋ࡚ๅࡾୖࡀࡾ࡛ 4 ㈉ࡲ࡛ࠊ⦅㞟ጤဨࡀ౫㢗ࡋࡓ⥲ㄝ ࠾࠸࡚ࡣࡑࡢ౫㢗ࡢ⠊ᅖࠊࡲࡓᢏ⾡ሗ࿌࠾࠸࡚ࡣๅࡾୖࡀࡾ 1 㡫ࡲ࡛ ࡢ༳ๅ㈝ࡣࠊཎ๎ࡋ࡚ᮏᏛࡀ㈇ᢸࡍࡿࠋ 㸵㸬ᥖ㍕ࡀỴᐃࡋࡓㄽᩥ➼ࡣࠊࡑࡢ᭱⤊✏ࡢᥦฟࡶᥦฟཎ✏ྠ୍ ෆᐜࢆࠊࢸ࢟ࢫࢺࣇࣝཬࡧㄽᩥసᡂ⏝ࡋࡓࢯࣇࢺࡢᶆ‽ⓗ࡞グ 㘓᪉ἲ࡛グ㘓ࡋࡓ CD-ROM ࠊ࠶ࡿ࠸ࡣ㟁Ꮚ࣓࣮ࣝࡢῧࣇࣝ㸦㸳 MB ௨ୗ㸧࡚ᥦฟࡍࡿࠋ 㸶㸬ᥖ㍕ࡢỴᐃࡋࡓㄽᩥ➼ࡢⴭ⪅ࡼࡿᰯṇࡣ 1 ᅇࡍࡿࠋⴭ⪅ࡣᣦᐃࡉ ࢀࡓᮇ㝈ෆᰯṇࢆ⤊ࡋ࡞ࡃ࡚ࡣ࡞ࡽ࡞࠸ࠋࡲࡓࠊⴭ⪅ᰯṇ࡛ࡢㄽᩥ ࡢෆᐜࡢኚ᭦ࡣㄆࡵࡽࢀ࡞࠸ࠋ 㸷㸬 ࠕBrachyspiraࠖᥖ㍕ࡉࢀࡓㄽᩥ➼ࡢⴭసᶒࡣࠊูつᐃࡋ࡞࠸㝈ࡾࠊ Brachyspira 2015;5(1): 45 Brachyspira 2015:5(1):46 ࡑࡢ㟁Ꮚࠊ࡛࢜ࣥࣛࣥࡢ⏝ࢆྵࡵ࡚ࡍ࡚᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ ᖐᒓࡍࡿࠋ 㸯㸮㸬ูๅࡾࡣ 50 㒊༢࡛ⴭ⪅ᰯṇࡢ㏉㏦Ⅼ⏦ࡋ㎸ࡴࠋ 㸯㸯㸬࣮࢝ࣛ༳ๅཬࡧูๅࡾࡘ࠸࡚ࡣู㏵༳ๅ㈝ࢆㄳồࡍࡿࡇࡀ࠶ࡿࠋ ᇳ➹せ㡿 ㄽᩥࡣⱥᩥ࡛సᡂࡍࡿࡇࡀᮃࡲࡋ࠸ࡀࠊⱥᩥࡢᢞ✏つᐃ➼ࠊཬࡧ௨ୗ ࡢグ㍕ࢆཧ⪃ࡋ࡚ᩥ࡛సᡂࡋࡓㄽᩥ➼ࡢᢞ✏ࢆㄆࡵࡿࠋⱥㄒࢆ㏻ᖖ ⏝ゝㄒࡋ࡞࠸ⴭ⪅ࡣࠊnative speaker ➼ࡼࡿ㐺ษ࡞ⱥᩥᰯṇࢆ⾜ࡗࡓ ࡢࡕᢞ✏ࡍࡿࡶࠊ㐺ษᛮࢃࢀࡿⱥᩥグ㍕ࡣࠊᰝㄞ࠶ࡓࡾࠊಟ ṇࡸཷ⌮ࡢᑐ㇟࡞ࡿᜍࢀࡀ࠶ࡿࠋ ࡞࠾ࠊཎ✏ࡢసᡂᙜࡓࡗ࡚ࡑࡢࢫࢱࣝ➼ࡣࠊⱥᩥࡢᢞ✏つᐃ 㸦Instructions to Authors㸧ཎ๎ࡋ࡚ᚑ࠺ࠋ 㸯㸬ᩥ࡛ࡢᢞ✏㝿ࡋ࡚ࡣࠊᚲࡎⱥᩥ࡛సᡂࡋࡓࠊࢱࢺࣝࠊⴭ⪅ྡࠊ ⴭ⪅ࡢᡤᒓࠊఫᡤࠊ࣮࣮࢟࣡ࢻࠊࣛࣥࢽࣥࢢࢱࢺࣝཬࡧせ᪨ࢆࠊㄽᩥ ࡶᥦฟࡍࡿࠋ 㸰㸬ᩥࡢཎ✏ࡣཎ๎ࡋ࡚ࠊᖖ⏝₎Ꮠࠊ⌧௦௬ྡ࡙࠸ࡼࡾࠊⓑⰍࡢ B5 ∧ࡢ⏝⣬ࠊ1 ⾜ 30 ᩥᏐࠊ1 㡫 30 ⾜࡛࣮࣡ࣉࣟࢯࣇࢺࡼࡗ࡚స ᡂࡍࡿࠋ 㸱㸬ᅗࠊ⾲ࠊ┿➼ࡣࡍ࡚ B5 ∧ࡢ⏝⣬ࢆ⏝࠸ 1 ௳ࡎࡘู⣬సᡂࡍࡿࠋ ࡲࡓࠊᅗࠊ⾲ࠊ┿➼ࡢ⾲㢟ཬࡧㄝ᫂ࡣู⣬ࡲࡵ࡚グ㍕ࡍࡿࠋ 㸲㸬ᅗࠊ⾲ࠊ┿➼ࡣࠊࡑࡢࡲࡲ┿〇∧㸦⦰ᑠࡍࡿሙྜࡶ࠶ࡿ㸧⪏࠼ ࡽࢀࡿࡶࡢࢆᥦฟࡍࡿࠋ༳ๅ㝿ࡋࠊࢺ࣮ࣞࢫ➼ࢆᚲせࡍࡿሙྜࠊࡑ ࡢ㈝⏝ࡣⴭ⪅ࡢ㈇ᢸࡍࡿࠋ 㸳㸬ㄽᩥࡢᵓᡂࡣཎ๎ࡋ࡚ࠊࢱࢺࣝ㡫ࠊせ᪨ࠊᮏᩥࠊᘬ⏝ᩥ⊩ࠊᅗࡢ ⾲㢟ཬࡧㄝ᫂ࠊ⾲ࠊᅗࡋࠊྛࠎู⣬సᡂࡋࠊ㡫␒ྕࢆ㏻ࡋ࡚ࡘࡅࡿࠋ a㸬ࢱࢺࣝ㡫㸦➨ 1 㡫㸧ࡣࠊࢱࢺࣝࠊⴭ⪅ࡢྡ๓࣭ᡤᒓࠊ㐃⤡ ㈐௵⪅ࡢẶྡ࣭ఫᡤ࣭㟁ヰ࣭ࣇࢵࢡࢫ࣭㟁Ꮚ࣓࣮ࣝࢻࣞࢫࠊ࣮࢟ ࣮࣡ࢻ㸦3㹼5 ಶ㸧ࢆグ㍕ࡍࡿࠋ 㹠㸬せ⣙ࡣࠊ➨ 2 㡫సᡂࡍࡿࠋ Brachyspira 2015:5(1):47 Brachyspira 2015;5(1): 46 㹡㸬ᮏᩥࡢᵓᡂࡣཎ๎ࡋ࡚ࠊ⥴ゝࠊᐇ㦂᪉ἲࠊ⤖ᯝࠊ⪃ᐹࠊ⤖ㄽࡋࠊ ㅰ㎡ࡣู㡯┠ࢆࡶ࠺ࡅࡎᮏᩥࡢ᭱ᚋ 1 ⾜✵ⓑࢆࡗࡓᚋࠊ⡆₩グ ㍕ࡍࡿࠋ 㹢㸬ᘬ⏝ᩥ⊩ࡢグ㍕ࡣཎ๎ࡋ࡚ⱥᩥࡢࡶࡢᚑ࠺ࡀࠊሗᩥ➼ࡀ᪥ᮏㄒ ࡛సᡂࡉࢀ࡚࠸ࡿሙྜࡢࡳࠊ᪥ᮏㄒࡼࡿⴭ⪅Ặྡࠊㄅྡ➼ࡼࡗ࡚ グ㍕ࡍࡿࠋ 㸴㸬ྜ≀ྡࡣࠊཎ๎ࡋ࡚ⱥྡࢆ⏝ࡍࡿࠋࡓࡔࡋࣝࢥ࣮ࣝࠊࣅࢱ࣑ ࣥ➼እᅜㄒ࡛⌧ᅾ័⏝ࡉࢀ࡚࠸ࡿࡶࡢࡣࠊ࢝ࢱ࢝ࢼ᭩ࡁࡍࡿࠋ 㸵㸬ᚤ⏕≀ྡࡸື᳜≀ྡࡣ࢝ࢱ࢝ࢼ᭩ࡁࡋࠊࡑࡢᏛྡࡣࢱࣜࢵࢡ㸦ᩳ య㸧ࡍࡿࠋ ᢏ⾡ሗ࿌ᢞ✏つᐃ ᢏ⾡ሗ࿌㸦Technical Note㸧ࡣࠊᚑ᮶ࡢㄽᩥᙧᘧࡢሗ࿌࡞ࡌࡲ࡞࠸ࡶ ࡢ࡛ࡢ◊✲⪅ࡗ࡚᭷┈࡞ࠊࡕࡻࡗࡋࡓᐇ㦂ᢏ⾡ୖࡢᕤኵࠊኚ᭦ࠊ ὀពⅬ࠶ࡿ࠸ࡣኻᩋ➼ࢆ⡆₩ࡲࡵࡓࡶࡢࢆᥖ㍕ࡍࡿࠋࡃࠊᚑ ᮶ࡢሗ࿌࡛ࡣᇙࡶࢀ࡚ࡋࡲ࠺ࠊࠕࡇ࠺ࡍࡿୖᡭࡃ⾜࡞࠸ࠖ࠸ࡗࡓኻ ᩋࡶࠊ᭷┈࡞ሗࡋ࡚✚ᴟⓗᥖ㍕ࡍࡿࠋ ᇳ➹ୖࡢὀពⅬ 㸯㸬ᢏ⾡ሗ࿌ࡣ≉ࡑࡢ᭩ᘧࢆᐃࡵ࡞࠸ࡀࠊ⡆₩࡞⾲㢟ࠊⴭ⪅ྡཬࡧᡤᒓ࣭ 㐃⤡ඛࠊሗ࿌ࡢᮏᩥࠊᚲせ࡞ᅗࠊ⾲ࠊཬࡧཧ⪃ᩥ⊩ࢆྵࡴࡶࡢࡍࡿࠋ 㸰㸬ᢏ⾡࡛࠶ࡿࡢ࡛ࠊㄞ⪅ࡼࡗ࡚⌧ࡍࡿࡇࡀྍ⬟࡞ࡿ༑ศ࡞ ሗࡀグ㍕ࡉࢀ࡚࠸ࡿᚲせࡀ࠶ࡿࠋ 㸱㸬ⴭ⪅ࡀ⊂⮬ぢ࠸ࡔࡋࡓࡾ☜ㄆࡋࡓ▱ぢ࡛ࠊ ࠕBrachyspiraࠖࡢㄞ⪅ ᭷⏝࡞ࡶࡢ࡛࠶ࢀࡤࠊ᪤ሗࠊࡶࡋࡃࡣᑗ᮶ࡢሗᩥࡢ୍㒊࡞ࡿࡶࡢ ࡛࠶ࡗ࡚ࡶࡼ࠸ࠋ 㸲㸬ᮏᩥ୰࡛ࡢ㏙ㄒࠊ༢➼ࡢグ㍕᪉ἲࡣࡢㄽᩥ➼ࡢつᐃࢆ‽ᣐࡍࡿࠋ Brachyspira 2015;5(1): 47 Brachyspira 2015:5(1):48 Instructions to Authors Aims and Scope ‘Brachyspira’is issued once a year, one volume a year, by the Japanese Society of Brachyspira and the purpose of the journal is to publish results and information relevant to Brachyspira. ‘Brachyspira’provides a medium for publication of review, original results, proceedings of special lectures, symposia and workshops to the meeting of Japanese Society of Brachyspira and short technical note. (See special information for technical note) Submission The manuscript must be written either in clear, grammatical English or Japanese, and authors are requested to submit their original manuscript and figures with two clear copies to Editor-in-Chief. Dr. Sho Ogata Department of Pathology and Laboratory Medicine, National Defense Medical College 3-2 Namiki, Tokorozawa, Saitama, 359-8513 Japan Tel +81-4-2995-1505 Fax +81-4-2996-5192 E-mail: [email protected] Manuscript must be submitted with a cover letter from the corresponding author implies that the all authors have approved the paper for release and are in agreement with its content. The authors are encouraged to be a member of the Japanese Society of Brachyspira but are not required. The submitted manuscript will be assigned to an editor and reviewed by independent reviewers. Editors may ask authors to revise the manuscript before accept for publication or rejection. Submission of original paper implied that it has not been published previously, that is not under consideration for publication elsewhere, and that if accepted it will not be Brachyspira 2015;5(1): 48 Brachyspira 2015:5(1):49 published elsewhere in the same form in any language, without the written consent of the Society. There are no page charges for first four printed pages and the authors are required to pay for extra page ¥10,000 per page. Preparation of Manuscript in English The manuscript must be type-written, double-spaced, with wide margins (minimum 25 mm all side on one side of good quality white B5 (182x257 mm) paper. Good quality typescripts with a font size of 12 pt are required. The corresponding author should be identified in including a fax number and email address. Authors are advised to consult a recent issue of the journal for style if possible. The editors reserve the right to adjust style to certain standards of uniformity. Submitted manuscript will not be returned to authors and Society cannot accept responsibility for damage or loss of papers so authors must retain a copy of their manuscript. The manuscript should be prepared following sections: 1) Title page; 2) Summary; 3) Text; 4) References; 5) Figure legend(s); 6) Table(s) with legend; 7) Figure(s). Acknowledgment(s) must be placed at the last part of Text with a line margin. Above each section must be written on separate sheets, each table and figure must be placed on separate sheet(s) and all pages must be numbered consecutively. Japanese author(s) are requested to attach a separate sheet with a Japanese title, name(s) and affiliation(s) and address(es) of author(s) in Japanese and Japanese summary of the manuscript. The authors who are not native in English are advised to be checked their manuscript by person who are talented in English before submission (Native speaker correction). Title page The first page should show: 1) the title; 2) name(s) of author(s); 3) affiliation(s) and address(es); 4) the name, address, phone and fax number and email address of the author to whom all correspondence should be addressed; 5) key words (3 to 5). Use one complete title rather than a title and subtitle. Brachyspira 2015;5(1): 49 Brachyspira 2015:5(1):50 Summary Summary should not exceed 300 words. Abbreviation can be used. Text The text usually contains, Introduction, Materials and Methods, Results and Discussion. Author(s) may prepare a combined “Results and Discussion” and author(s) may prepare a Conclusion. Headings: Introduction, Materials and Methods, Results and other major headings should be typed with one line margins. Major section of the text can be divided into subheads. Subheads should be typed in flush left on the line with italic face. Note: In review and proceedings, it is not necessary to follow above orders. Author(s) can choose most appropriate order to discuss the subject. References Submitted papers or unpublished oral presentations may not be listed as references; cite them in text as unpublished data or personal communications. Cite all references to previously published papers or paper in press in alphabetical order in text with number like, [1], [2,3], or [4-6]. List references numerically in “References” in styles of exampled shown below. If a number of authors exceeds three, please write the first three with “, et al”. Abbreviation of journal title should be standard, along such as the Index Medicus. Journals: 1. Johansson KE, Duhamel GE, Bergsjo B, et al. 2004. Identification of three clusters of canine intestinal spirochaetes by biochemical and 16S rDNA sequence analysis. J Med Microbiol 53: 345-50. Chapter of book: 2. Rotterdam H. Intestinal spirochetosis. 1997. In: Conner DH, Chandler FW, eds. Pathology of Infectious Diseases. Volume 1. Stamford, CT: Appleton & Lange; pp.583-8. Brachyspira 2015;5(1): 50 Brachyspira 2015:5(1):51 Book: 3. Smith JE, Moss MO. 1985. Mycotoxins, Formation, analysis and significance. Chichester, UK: John Wiley & Sons Ltd. Table Each table must be typed on separate sheet. Tables should be numbered consecutively and given a suitable capitation. Footnotes to tables should be types below the table and should be referred to by superscript lowercase letters (like a,b,c ). No vertical rules should be used. Tables should not duplicate results presented elsewhere in the manuscript. Figure Each figure must be made in separate sheet. All figures should be provided in camera-ready form, suitable for reproduction (which may include reduction) without retouching. Original photographs must be supplied as they are to be reproduced (black and white). Color photographs may be used with extra charge for author. 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Technical note This is a special section which provides useful but not complete information for Brachyspira research. The authors should prepare the note following the instruction to authors for ‘Brachyspira’and special information for the note below. Special information for Technical note In this section, an improvement of technique(s) concerning Brachyspira research can be reported as a short form. The authors should submit three copies of note to the Society with cover letter from corresponding author. The note should contain title, name affiliation and address of author(s), text, figure and table and references. The editor will review the note and may ask authors to revise the note before accept for publication or reject. Brachyspira 2015;5(1): 53 Brachyspira 2015:5(1):54 ົሗ࿌ Newsletter from the office ➨ ᅇ ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ ᖿ㆟㘓 ሙᡤ㸸Ⲉᇛ┴┴༡⏕ᾭᏛ⩦ࢭࣥࢱ࣮ ᪥㸸ᖹᡂ ᖺ ᭶ ᪥ ༗ᚋ 㹼 ศ ฟᖍ⪅㸸㊊❧ࠊ⥴᪉ࠊᮎྜྷࠊᒾᮏࠊୗᆅࠊ➉⃝ࠊᯘࠊ୰ᓥ Ϩ ⥲㆟ࡍࡿ㡯ࡘ࠸࡚ 㸯㸬ሗ࿌㡯 㸯㸧άືሗ࿌㸦୰ᓥᗢົᖿ㸧 ู࣭⣬㈨ᩱᇶ࡙ࡁሗ࿌ࡀ࠶ࡗࡓࠋ 㸰㸧Ꮫㄅ㛵㐃ሗ࿌㸦⥴᪉㛗ව⦅㞟ጤဨ㛗㸧 ࣭➨ ᕳࡘ࠸࡚ࠊ 㢟ࡢᢞ✏ㄽᩥࡀ࠶ࡾࠊ 㢟ࡘ࠸࡚ࡣᰝㄞ῭ ࡳ࡛ ᭶㡭ࢆ┠ᶆⓎหணᐃ࡛࠶ࡿࠋ 㸱㸧ᅜ㝿ሗ㛵㐃ሗ࿌㸦Ლཎᩗኴᅜ㝿Ꮫᢸᙜᖿ㸧 ࣭ࠕ WK ,QWHUQDWLRQDO &RQIHUHQFH RQ &RORQLF 6SLURFKDHWDO ,QIHFWLRQVLQ$QLPDOVDQG+XPDQVࠖࡀ ᭶ ࠊ ᪥ⱥᅜࡢ 6XUUH\ Ꮫ࡛㛤ദࡉࢀࡿ᪨ࡀࠊᲚཎᖿኚࢃࡾ㊊❧㛗ࡽሗ࿌ࡀ࠶ࡗ ࡓࠋ 㸰㸬ᑂ㆟㡯 㸯㸧ィ࣭┘ᰝሗ࿌㸦㊊❧㛗௦⌮ࠊ➉⃝┘㸧 ู࣭⣬㈨ᩱᇶ࡙ࡁィሗ࿌ࡀ࠶ࡾࠊࡑࡢᚋ➉⃝┘ࡽ┘ᰝሗ࿌ ࡀ࠶ࡗࡓࠋ ࣭Ꮫ⾡㞟ࡣ⊂❧ィ࡛࠶ࡿࡀࠊᏛࡽຓࡀฟ᮶࡞࠸ࡢⓎ ㆟ࡀ࠶ࡾࠊᚋຓࡍࡿ㔠㢠ࡶྵࡵࠊ๓ྥࡁ᳨࡛ウࡋ࡚࠸ࡃࡇ ࡞ࡗࡓࠋ ࣭ဨࡢ㈝ᮍ⣡ࠊ࠾ࡼࡧᐤ㔠➼ࡘ࠸࡚༠㆟ࡋࡓࠋ Brachyspira 2015;5(1): 54 Brachyspira 2015:5(1):55 㸰㸧ᙺဨࡘ࠸࡚ ࣭ᾏ㐨Ꮫ㝔ࡢ㛫㒊ඞ⿱Ặࢆᖿࡋ࡚㏄࠼ࡿࡇࡀᢎࡉࢀ ࡓࠋ 㸦࣭ື≀⾨⏕◊✲ᡤࡢୗᆅၿ༤Ặࢆᖿࡋ࡚㏄࠼ࡿࡇࡣࠊ➨ ᅇ┘࡛ࡍ࡛ᢎࡉࢀ࡚࠸ࡓࠋ 㸧 㸱㸧ḟᅇࡢᏛ⾡㞟㛤ദࡘ࠸࡚ ࣭➨ ᅇᏛ⾡㞟ࡣᮾி་ࡢᒾᮏᖿࡀ㛗ࡋ࡚ࠊሙᡤࡣ 㜿ぢ⏫ࡢᮾி་Ⲉᇛ་⒪ࢭࣥࢱ࣮࡛ࠊᮇࡣ ᭶୰ᐇࡍࡿࡇ ࡀᢎࡉࢀࡓࠋ 㸲㸧ࡑࡢ ձ Ꮫ㈹ೃ⿵⪅ࡢ௳ ࣭ḟᅇࡢ⥲࠾࠸࡚ࠊᏛ㈹ࢆ⥴᪉⾪ඛ⏕ࠊዡບ㈹ࢆᮾᒣṇ ᫂ඛ⏕ᤵࡍࡿࡇࡀᢎࡉࢀࡓࠋ ղ 㹌㹎㹍ἲே㛵ࡍࡿ௳ ࣭᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛᑐࡋࠊ⌧ᅾᐤࢆࡋ࡚ࡃࡔࡉࡿே㸦 ♫㸧ࡀᒃࡿࠋࡑࡢ᪉ࡓࡕࡢ⛯ⓗඃ㐝ᥐ⨨ࢆཷࡅࡿᏳࡃࡍࡿࡇ ࡼࡾᐤ⾜ⅭࡀࡸࡾࡸࡍࡃࡍࡿࡓࡵࡶࠊᚋࠊᙜᏛࢆ㹌㹎㹍 ἲேࡋ࡚⾜ࡃᚲせࡀ࠶ࡿࡢ࡛ࡣ࡞࠸ࡢㄝ᫂ࡀ㊊❧㛗 ࡽ࠶ࡾࠊᚋ᳨ウࡋ࡚࠸ࡃࡇ࡞ࡗࡓࠋ Brachyspira 2015;5(1): 55 Brachyspira 2015:5(1):56 ➨ ᅇ ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ ᖿ㆟㘓 ሙᡤ㸸ࡘࡃࡤᅜ㝿㆟ሙ࣭➨ ㆟ᐊ ᪥㸸ᖹᡂ ᖺ ᭶ ᪥ ༗ᚋ 㹼 ศ ฟᖍ⪅㸸㊊❧ࠊ୰ᮧࠊ⥴᪉ࠊᮎྜྷࠊᒾᮏࠊ➉⃝ࠊྜྷ⃝ࠊ㛫㒊ࠊ୰ᓥ Brachyspira 2015;5(1): 56 Brachyspira 2015:5(1):57 Ϩ ⥲㆟ࡍࡿ㡯ࡘ࠸࡚ 㸯㸬ሗ࿌㡯 㸯㸧άືሗ࿌㸦୰ᓥᗢົᖿ㸧 ู࣭⣬㈨ᩱᇶ࡙ࡁሗ࿌ࡀ࠶ࡗࡓࠋ 㸰㸧Ꮫㄅ㛵㐃ሗ࿌㸦⥴᪉㛗ව⦅㞟ᖿ㸧 ࣭⥴᪉⦅㞟ᖿኚࢃࡾ㊊❧㛗ࡽࠊ➨ ᕳࡘ࠸࡚ࠊ 㢟ࡢᢞ ✏ㄽᩥࡀ࠶ࡿࡇࡀሗ࿌ࡉࢀࡓࠋ 㸱㸧ᅜ㝿ሗ㛵㐃ሗ࿌㸦Ლཎᩗኴᅜ㝿Ꮫᢸᙜᖿ㸧 ࣭ࠕ WK ,QWHUQDWLRQDO &RQIHUHQFH RQ &RORQLF 6SLURFKDHWDO ,QIHFWLRQVLQ$QLPDOVDQG+XPDQVࠖࡀ ᭶ ࠊ ᪥ⱥᅜࡢ 6XUUH\ Ꮫ࡛㛤ദࡉࢀࠊ㊊❧㛗ࡢࢢ࣮ࣝࣉࠊ⥴᪉㛗ࠊ࠾ࡼࡧ㛫㒊ᖿ ࢢ࣮ࣝࣉࡀཧຍࡋࠊḟᅇࡣ ᖺᚋࢹ࣐࣮ࣥࢡ࡛㛤ദࡉࢀࡿ᪨ࠊᲚ ཎᖿኚࢃࡾ㊊❧㛗ࡽሗ࿌ࡀ࠶ࡗࡓࠋ 㸰㸬ᑂ㆟㡯 㸯㸧ィ࣭┘ᰝሗ࿌㸦㊊❧㛗௦⌮ࠊ➉⃝┘㸧 ู࣭⣬㈨ᩱᇶ࡙ࡁィሗ࿌ࡀ࠶ࡾࠊࡑࡢᚋ➉⃝┘ࡽ┘ᰝሗ࿌ ࡀ࠶ࡾࠊᑂ㆟ࡢୖࠊᢎㄆࡉࢀࡓࠋ 㸰㸧ᙺဨࡘ࠸࡚ ࣭ᐑᓮ࣭୕⃝ᖿࡽ㏥ᒆࡀᥦฟࡉࢀࡓࡇక࠸ࠊᙺဨࡽ ㏥ࡃ᪨ࡢㄝ᫂ࡀ୰ᓥᖿࡽㄝ᫂ࡀ࠶ࡾࠊᢎࡉࢀࡓࠋ 㸱㸧ḟᅇࡢᏛ⾡㞟㛤ദࡘ࠸࡚ ࣭➨ ᅇᏛ⾡㞟ࡣಙᕞ㸦 ᭶ࡽி㸧ࡢྜྷ⃝ᖿࡀ 㛗ࡋ࡚ࠊሙᡤࡣி࢟ࣕࣥࣃࢫ࡛ࠊᮇࡣ ᭶୰ᐇࡍࡿࡇ ࡀᢎࡉࢀࡓࠋ 㸲㸧ࡑࡢ ձ ୰ᮧ㢳ၥࡽࠊᖺ㱋ࡼࡾ㢳ၥࡽ㏥ࡁࡓ࠸᪨ࡢ⏦࿌ࡀ࠶ࡾࠊ ᑂ㆟ࡢୖࠊᢎࡉࢀࡓࠋࡓࡔࠊᚋࡶ㛫ࡢ㒔ྜࡀࡅࡤࠊᏛ⾡ 㞟ࡢཧຍࢆ࠾㢪࠸ࡋࡓࠋ ௨ୖ Brachyspira 2015:5(1):58 Brachyspira 2015;5(1): 57 ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛ๎ >⥲๎@ ➨୍᮲ ᮏࡢྡ⛠ࢆ᪥ᮏࣈࣛ࢟ࢫࣆࣛᏛࡍࡿࠋ ➨᮲ ᮏࡣࠊࣈࣛ࢟ࢫࣆࣛཬࡧ㛵㐃ᝈࡘ࠸࡚ࡢ▱㆑ࡢὶࠊ◊ ✲᥎㐍ࢆࡋࠊဨ┦ࡢぶ╬ࢆᅗࡿࡇࢆ┠ⓗࡍࡿࠋ ➨୕᮲ ᮏࡣ๓᮲ࡢ┠ⓗ㐩ᡂࡢࡓࡵࠊḟࡢᴗࢆ⾜࠺ࠋ 㸯㸬 ဨࡢ▱㆑ྥୖࡢࡓࡵࡢ㞟ࡢ㛤ദ 㸰㸬 ဨ┦ࡢぶ╬ࢆࡣࡿࡓࡵࡢ㞟ࡢ㛤ദ 㸱㸬 ㄅࡢⓎ⾜㓄ᕸ 㸲㸬 ࡑࡢᚲせ࡞㡯 >ဨ@ ➨ᅄ᮲ ᮏࡢ㊃᪨㈶ᡂࡋ࡚ຍධࡋࡓࡶࡢࢆဨࡍࡿࠋ ➨᮲ ဨࡣᮏࡢ๎ᚑ࠸ࠊࡢ㐠Ⴀ༠ຊࡋࠊ㈝ࢆ⣡ධࡍࡿ⩏ ົࢆ㈇࠺ࠋణࡋࠊ㈝ࡢ㢠ཬࡧ⣡ධ᪉ἲࡣ๎ᐃࡵࡿࠋ >ྡဨ@ ➨භ᮲ ࣈࣛ࢟ࢫࣆࣛࡑࡢ㛵㐃ᝈࡘ࠸࡚ࡢ◊✲▱㆑ࡢྥୖⴭ ࡋ࠸ᴗ⦼ࡢ࠶ࡿࡶࡢࢆࠊྡဨࡍࡿࠋ >ᙺဨ@ ➨᮲ ᮏḟࡢᙺဨࢆ⨨ࡃࠋ 㸯㸬 㛗 㸯ྡ 㸰㸬 㛗 㸱ྡ௨ෆ 㸱㸬 ᖿ ⱝᖸྡ ➨ඵ᮲ 㛗ࡣ⥲࡛㑅ฟࡋࠊ㛗ࠊᖿࡣ㛗ࡀ᥎⸀ࡋ⥲ࡢᢎㄆࢆ ⤒࡚Ỵᐃࡍࡿࠋ ➨᮲ ᙺဨࡢ௵ᮇࡣ㸰ᖺࡍࡿࠋణࡋࠊ㔜௵࠶ࡿ࠸ࡣ௵ࡣጉࡆ࡞࠸ࠋ >⥲@ ➨༑᮲ ᖺ㸯ᅇဨ⥲ࢆ㛤ദࡍࡿࠋ Brachyspira 2015;5(1): 58 Brachyspira 2015:5(1):59 >ᖿ@ ➨༑୍᮲ ᖿࡣᖿࢆᵓᡂࡋࠊᮏࡢົ୍⯡ࡢලయⓗ㡯ࢆᑂ㆟Ỵ ᐃࡍࡿࡶࡑࢀࢆᇳ⾜ࡍࡿࠋ >⾲ᙲ@ ➨༑᮲ ᮏࡣࠊဨࡢࡍࡄࢀࡓᴗ⦼ᑐࡋ࡚Ꮫ⾡㈹ࡢཷ㈹ࢆ⾜࠺ࠋ ๎ 㸯㸬 ィᖺᗘ ᮏࡢィᖺᗘࡣ㸯᭶㸯᪥㹼㸯㸰᭶㸱㸯᪥ࡲ࡛ࡍ ࡿࠋ 㸰㸬 ㈝ ಶேဨࡣᖺ㸳㸪㸮㸮㸮ࠊ㈶ຓဨࡣ୍ཱྀ㸯㸮㸪㸮㸮㸮 ௨ୖࢆ๓ᖺᗘ㸯㸰᭶ᮎࡲ࡛๓⣡ࡍࡿࠋ 㸱㸬 ๎ኚ᭦ ᮏࡢ๎㸦⥲๎ཬࡧ๎㸧ࡣࠊ⥲࠾࠸࡚ጤ௵≧ ࠾ࡼࡧฟᖍဨࡢ㸱ศࡢ㸰ࡢ㈶ᡂࢆᚓࡓሙྜኚ᭦ࡍࡿࡇࡀ࡛ࡁ ࡿࠋ 㸲㸬 ົᒁ ົᒁࢆḟ࠾ࡃࠋ ࠛ㸱㸮㸮㸫㸮㸱㸷㸱 Ⲉᇛ┴✄ᩜ㒆㜿ぢ⏫୰ኸ㸱㸫㸰㸯㸫㸯Ⲉᇛ Ꮫ㎰Ꮫ㒊ື≀ಖ⾨⏕◊✲ᐊ 㟁ヰ␒ྕ 㸮㸰㸷㸫㸶㸶㸶㸫㸶㸳㸵㸰 㹄㸿㹖␒ྕ 㸮㸰㸷㸫㸶㸶㸶㸫㸶㸳㸵㸰 㸳㸬 ⾜᪥ ࡇࡢᨵṇࡣᖹᡂ㸰㸯ᖺ㸵᭶㸱㸯᪥ࡽ⾜ࡍࡿࠋ 賛 助 会 員 （50 音順） 医 学 科 学 ゾ エ 日 日 中 技 テ 本 央 術 振 ィ 誌 興 機 ス・ジ 全 本 雑 薬 ハ 出 版 構（独 ャ 工 パ 業 ム 株 式 立 行 政 ン 株 式 株 株 式 式 会 社 法 人） 会 会 会 社 社 社 ノ バ ル テ ィ ス ア ニ マ ル ヘ ル ス 株 式 会 社 ベーリンガーインゲルハイムベトメディカジャパン株式会社 三 菱 ガ ス Meiji Seika フ 化 ァ 学 ル 株 マ 式 株 式 会 会 社 社 Brachyspira 第 5 巻第 1 号 2015 年 1 月 30 日 平成 27 年 1 月 30 日 発 行 者 日 本ブラキスピラ 学 会 会 長 足 立 吉 數 事務局 〒300–0393 茨城県稲敷郡阿見町中央 3- 21 -1 茨城大学農学部動物保健衛生研究室 振替口座：00100-0-678496 日本ブラキスピラ学会 一般会員会費 年額 5,000円 印 刷 所 佐 藤 印 刷 株 式 会 社 〒305–0051 茨城県つくば市二の宮 4 丁目4–21 TEL. 029–855–7622 FAX. 029–855–7490