1 FBC Lett., No. 45 (2014) （2014 年 7 月） 夢への責任 藤井 郁雄 齊藤

FBC Lett., No. 45 (2014)
2014
2
4
夢への責任
大阪府立大学大学院理学系研究科
7
藤井 郁雄
4 RNA ワールドから iPS 細胞へ -生命起源と細胞構築原理の探求京都大学 iPS 細胞研究所 白眉センター
齊藤 博英
9 バクテリア融合チャンバー -細胞の再構成に向けた人工物との融合田端 和仁
東京大学大学院工学研究科
14
14.
18.
京都大学大学院工学研究科
博士課程１年
東北大学多元物質科学研究所
博士課程２年
21.
25
九州大学大学院薬学研究院
助教
河
陸
上松 亮平
田畑 栄一
カリフォルニア工科大学留学体験記
Division of Chemistry and Chemical Engineering, California Institute of Technology
30
田村 朋則
第８回 バイオ関連化学シンポジウム
第 41 回 国際核酸化学シンポジウム（ISNAC2014）
第 24 回 バイオ・高分子シンポジウム
14-1 バイオ・高分子研究会
人工光合成国際会議 2014
第 17 回 生命化学研究会
受賞、異動
編集後記
1
No.45 (2014 July)
2008
2014
STAP
/
4
4
O
iPS
…
R
O
2
STAP
No.45 (2014 July)
O
O
2014
3
6
No.45 (2014 July)
[email protected]
1.
RNA
RNA
RNA
RNA
RNA
Jack Szostak
David Bartel
RNA
RNA
Szostak
RNA
RNA
tRNA
tRNA
RNA
RNA
RNA
RI
tRNA
1,2,3
RNA
4
4
No.45 (2014 July)
2. RNA5
RNA
2005
RNA
(RNA
)
mRNA
RNA
RNA
(RNA
)
RNA
(
)
1
1
RNA
RNA
mRNA
mRNA
(UTR)
mRNA
L7Ae
RNA
6
L7Ae
RNA (K-turn)
K-turn
mRNA
L7Ae
L7Ae
mRNA
3.
5
mRNA
mRNA
mRNA
RNA
mRNA
(
2)
7
mRNA
mRNA
2
5
5
RNA
No.45 (2014 July)
5
5
cis
UTR
UTR
mRNA
(2)
mRNA
(2)'
gene'of'interest
(1)
(1) 
RNA
'
$
(1)$
(2)$
4. RNA
RNA
RNA
8
RNA
RNA
RNA
OFF
ON
3
RNA
mRNA
mRNA
mRNA
(NMD)
NMD
mRNA
ON
OFF
ON
OFF
mRNA 1
1
6
ON
No.45 (2014 July)
ON'
.
.
OFF
(intron)
IRES
0
0
1
1
mRNA'
.
ON
(intron)
IRES
mRNA
5. RNARNAL7Ae-K-turn
L7Ae
60
K-turn RNA
L7Ae-K-turn
(
4
)
9
K-turn
L7Ae
60
AFM
RNA
1
L7Ae
(
10nm
RNA
7
4
)
RNA
No.45 (2014 July)
6.
RNA
2010
iPS
2011
iPS
RNA
RNA
RNA
RNA
RNP RNA-Protein
RNA
RNP
10
iPS
1.
Saito, H., Kourouklis, D. & Suga, H. An in vitro evolved precursor tRNA with aminoacylation
activity. EMBO J 20, 1797-1806 (2001).
2.
Saito, H. & Suga, H. A ribozyme exclusively aminoacylates the 3'-hydroxyl group of the tRNA
terminal adenosine. J Am Chem Soc 123, 7178-7179 (2001).
3.
Saito, H., Watanabe, K. & Suga, H. Concurrent molecular recognition of the amino acid and tRNA
by a ribozyme. RNA 7, 1867-1878 (2001).
4.
Saito, H. & Inoue, T. Synthetic biology with RNA motifs. Int J Biochem Cell Biol 41, 398-404
(2009).
5.
Saito, H. et al. Synthesis of functional proteins by mixing peptide motifs. Chem Biol 11, 765-773
(2004).
6.
Saito, H. et al. Synthetic translational regulation by an L7Ae-kink-turn RNP switch. Nat Chem Biol
6, 71-78 (2010).
7.
Endo, K., Stapleton, J.A., Hayashi, K., Saito, H. & Inoue, T. Quantitative and simultaneous
translational control of distinct mammalian mRNAs. Nucleic Acids Res 41, e135 (2013).
8.
Endo, K., Hayashi, K., Inoue, T. & Saito, H. A versatile cis-acting inverter module for synthetic
translational switches. Nat Commun 4, 2393 (2013).
9.
Ohno, H. et al. Synthetic RNA-protein complex shaped like an equilateral triangle. Nat Nanotechnol
6, 116-120 (2011).
10.
Hagiya, M., Konagaya, A., Kobayashi, S., Saito, H. & Murata, S. Molecular Robots with Sensors
and Intelligence. Acc Chem Res 47, 1681-1690 (2014).
8
No.45 (2014 July)
[email protected]
1.
2.
Craig Venter
[1]
1Mbp
Mycoplasma mycoides
Mycoplasma capricolum
Mycoplasma
mycoides JCVI-syn 1.0
1
9
No.45 (2014 July)
3.
?
2
mg/ml
10
1000
2
4.
?
2
1
5
m
1
1
digital ELISA
[2,3]
fL
10
fL
No.45 (2014 July)
1
[4]
1
2
3
GFP
a
3
GFP
5.
DNA
2
ATP
GFP
4
2
3
5
a
GFP
5mM ATP
ATP
ATP
DNA
DNA
GFP
6
GFP
GFP
GFP
11
4
GFP
1
No.45 (2014 July)
GFP
Background
GFP
DNA
DNA
6.
?
5
7
7.
6
GFP
7
12
No.45 (2014 July)
[1] D.G. Gibson, J.I. Glass, C. Lartigue, V.N. Noskov, R.Y. Chuang, M.A. Algire, G.A. Benders, M.G.
Montague, L. Ma, M.M. Moodie, C. Merryman, S. Vashee, R. Krishnakumar, N. Assad-Garcia, C.
Andrews-Pfannkoch, E.A. Denisova, L. Young, Z.Q. Qi, T.H. Segall-Shapiro, C.H. Calvey, P.P. Parmar,
C.A. Hutchison, 3rd, H.O. Smith, J.C. Venter, Creation of a bacterial cell controlled by a chemically
synthesized genome, Science, 329 (2010) 52-56.
[2] S. Sakakihara, S. Araki, R. Iino, H. Noji, A single-molecule enzymatic assay in a directly accessible
femtoliter droplet array, Lab on a Chip, 10 (2010) 3355-3362.
[3] S.H. Kim, S. Iwai, S. Araki, S. Sakakihara, R. Iino, H. Noji, Large-scale femtoliter droplet array for
digital counting of single biomolecules, Lab on a Chip, 12 (2012) 4986-4991.
[4] R. Watanabe, D. Fujita, K.V. Tabata, L. Yamauchi, N. Soga, S.H. Kim, H. Suga, H. Noji, High
throughput formation of sub-million lipid membrane arrays for measuring membrane protein activities,
proceedings of MicroTAS 2013, (2013) 1314-1316.
13
No.45 (2014 July)
[email protected]
2014
3
2014
4
Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular
response after laser-induced heating
T. Lei, A. F. Fernandez, R. Manchanda, Y. -C. Hung, and A. J. McGoron, Beilstein J. Nanotechnol. 2014, 5,
313-322.
100 nm
EPR
MRI
PET
700-900 nm
ICG
ICG
ICG
HIF-1
HIF-1
HIF-1
HIF-1
HSP
HSP
ICG
HSP
14
No.45 (2014 July)
PGMD
IR820
ICG
IR820-PGMD
PGMD
IR-820
IR820
IR820-PGDM
Figure 1
IR820-PGMD
24
MES-SA/Dx5
ROS
VEGF
HIF-1
IR820-PMGD
ICG
HIF-1
IR820-PMGD
Figure 1 IR820
Superparamagnetic hollow hybrid nanogels as a potential guidable vehicle system of stimuli-mediated
MR imaging and multiple cancer therapeutics
W. -H. Chiang, V. -T. Ho, H. -H. Chen, W. -C. Huang, Y. -F. Huang, S. -C. Ling, C. -S. Chern, and H. -C,
Chiu, Langmuir 2013, 29, 6434-6443.
DDS
MRI
SPIONs
SPIONs
SPIONs
PEG
MES
PAA
PNIPA
Figure 2
MES
pH
190 nm
-18.5 mV
SPIONs
pH
DOX
T2-type
MRI
MRI
pH
Figure 2
15
No.45 (2014 July)
in vivo
MRI
HeLa
SPIONs
DOX
DOX
HeLa
Figure 2
pH
SPIONs
MRI
SiO2@YBO3: Eu3+ hollow mesoporous spheres for drug delivery vehicle
G. Yang, S. Gai, F. Qu, and P. Yang, ACS Appl. Mater. Interfaces 2013, 5, 5788-5796
DDS
DDS
DDS
16
No.45 (2014 July)
DDS
Figure 3
Figure 3
(
DDS
)
200
90 nm
180 300 nm
TEOS
(HMSs@YBO3:Eu3+)
PBS
HMSs@YBO3:Eu3+
DOX
30-40%
HMSs@YBO3:Eu3+
DOX
DOX
pH
DOX
L929
HMSs@YBO3:Eu3+
200 µg/mL
MTT
DDS
HMSs@YBO3:Eu3+
DDS
17
No.45 (2014 July)
2
[email protected]
PS
1
2
Antisense therapies for cardiovascular/metabolic diseases
R. S. Geary, R. Crooke, S. Bhanot, W. Singleton, Drug Discov. Today Ther. Strateg., 2014, in press. (DOI:
10.1016/j.ddstr.2013.06.001)
RNA
RNase H
RNAi
Mipomersen KYNAMROTM
apoB-100
Gap motif
RNase H
RNAi
Phase 3
ALN-PCS
PCSK9
1
Phase 1
PCSK9
ALN-PCS
Base
O
LDL
HO
Base
R
O
O P
O
S
Base
Base
O
R
O
O P
S
O
O
n
O
O P
S
O
O
n
LDL
Base
R
O
O P
O
S
n
O
R
OH
R=
O
O
1. Gap motif
2
Ultrasmall gold nanoparticles as carriers for nucleus-based gene therapy due to size-dependent nuclear entry
S. Huo, S. Jin, X. Ma, X. Xue, K. Yang, A. Kumar, P. C. Wang, J. Zhang, Z. Hu, X. -J. Liang, ACS Nano,
2014, 5852-5862.
Triplex-forming oligonucleotides TFOs
TFO
DNA
DNA
DNase
Au-NPs
18
No.45 (2014 July)
50 nm
TFO
Tiopronin
nm
6 nm
Au-TIOP NPs
2a
10 nm
16 nm
2
2b
FITC
MCF-7
2 nm Au-FITC NP
3
9 nm
2 nm Au-TIOP NP
POY2T
TFO
MCCF-7
c-myc
Au-POY2T NPs,
24 h
2c
Au-POY2T NPs
c-myc
PCR
TFO POT2T
Au-POY2T NPs
3
80 %
20%
TFO
2 nm
(a)
(c)
(b)
2. (a) Au-NPs
American Chemical Society
2, 6 nm ; (b) Au-NPs
10, 16 nm (c) Au-POY2T
3. A 2 nm, B 6 nm, C 10 nm, D 16 nm Au-FITC NPs
; :
Au-FITC NPs; : MCF-7
Copyright 2014 American Chemical Society
19
Copyright 2014
4.
(Copyright 2014 American Chemical Society)
No.45 (2014 July)
Versatile RNA interference nanoplatform for systemic delivery of RNAs
K. Y. Choi, O. F. Silvestre, X. Huang, K. H. Min, G. P. Howard, N. Hida, A. J. Jin, N. Carvajal, S. W. Lee, J.
-I. Hong, X. Chen, ACS Nano, 2014, 8 (5), 4559-4570.
RNA
RNAi
RNAi
siRNA
miRNA
RNA
RNA
RNA
5βHA-NP
CD44
CD44
Zn(II)-dipicolylamine
DPA/Zn
HA-NP
RNA
HDz/RNA
RNA
HDz/RNA
CaP
CaP-HDz/RNA
5
CaP-HDz/RNA
CaP
DPA/Zn
RNA
RNA
CaP
RNA
HA-NP
HA-NP
CaP-HDz/RNA
RNA
in vivo
siRNA
RNAi
fLuc
HA-NP
siLuc
DPA/Zn
5. CaP-HDz/RNA
Control:
PBS,
Non-cording
(a)
Drug
(Optional)
CaP
RNA
(Copyright 2014 American Chemical Society)
(b)
sequence: siNC
fLuc
6
CaP-HDz/RNA
siRNA
RNAi
6. (a)
RNA
:
:
; (b)
3
(Copyright
2014 American Chemical Society)
20
3
No.45 (2014 July)
[email protected]
DNA
2008
4
photo affinity labeling
A propofol binding site on mammalian GABAA receptors identified by photolabeling
G. M. S. Yip, Z. -W. Chen, C. J. Edge, E. H. Smith, R. Dickinson, E. Hohenester, R. R. Townsend, K. Fuchs,
W. Sieghart, A. S. Evers, N. P. Franks
Nature Chem. Biol. 2013, 9, 715-720.
Propofol
1
1
GABA
γGABAA
5
GABAA
Cl–
5
β3
propofol
GABAA
β3
photo affinity labeling
trifluoromethyl diazirine
propofol
GABAA
Sf9
1
propofol
GABAA
α1
21
No.45 (2014 July)
ortho-propofol diazirine(o-PD)
β3
o-PD(dPD)
m/z
LC/MS
o-PD
dPD
1
m/z
1
o-PD
(
o-PD
MS
GABAA
2)
H267
β3
His Ala
o-PD
H267
propofol
propofol
β3
5
GABAA
1
β3
β3
propofol
GPCR G protein-coupled receptor
photo affinity labeling
Palladium-triggered deprotection chemistry for protein activation in living cells
J. Li, J. Yu, J. Zhao, J. Wang, S. Zheng, S. Lin, L. Chen, M. Yang, S. Jia, X. Zhang, P. R. Chen, Nature
Chem. 2014, 6, 352-361.
1
chemical rescue strategy
ε-NH2
Lys
propargyl
(Pd)
(
3)
rhodamine 110
(
NH2
propargyloxycarbonyl(Proc)
4a)
(Pd
22
allyloxycarbonyl(Alloc)
)
(
4b) Proc
No.45 (2014 July)
Pd
C5
phosphothreonine lyase
1
OspF
OspF
)
OspF
Lys134
lyase(
Lys134
ε-NH2
Proc
Lys
(OspF-K134-Proc-Lys)
OspF-K134-Proc-Lys
(
(Proc-Lys)
HeLa
Pd
Erk(p-Erk)
p-Erk
(
OspF-K134-Proc-Lys
5b
(
Proc-Lys
5a)
(
1,2)
5b
5b
4) Pd
Proc-Lys
3,6,7) OspF-K134-Proc-Lys
Pd
The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry
M. C. Jürgens, J. Vörös, G. J. P. Rautureau, D. A. Shepherd, V. E. Pye, J. Muldoon, C. M. Johnson, A. E.
Ashcroft, S. M. V. Freund, N. Ferguson
Nature Chem. Biol. 2013, 9, 540-547.
B
HBV
3
1
L
protein
2
1
+ preS2
PreS
cytosol
e-preS L
preS1
i-preS L
PreS
γ2-adaptin
6a
lumen
6b
i-preS L
EAR
6c
γ2-EAR
23
γ
No.45 (2014 July)
i-preS L
γ2-adaptin
NMR
ITC
i-preS L
i-preS L
γ2-EAR
PreS
PreS1
δ
γ2-EAR PreS1
HSQC heteronuclear single quantum coherence
γ
chemical shift perturbation CSP
PreS1
29-41
δ
δ
7
δ
γ2-EAR
29-41
preS1
ITC
γ2-EAR
9-16
γ2-EAR
59-68
3
γ2-EAR
29-36
1
15
PreS1
PreS1
1
N- H heteronuclear NOE ion mobility spectrometry-mass spectrometry
IMS-MS
IMS-MS
IMS
m/z
PreS1
extended form
intrinsically disordered protein; IDP
NMR
γ2-EAR
15
IMS-MS
1
N- H heteronuclear NOE
2
γ2-EAR-preS1
preS1
γ2-EAR
HBV
NMR ITC IMS-MS
IDP
IDP
2
IDP
IDP
24
No.45 (2014 July)
[email protected]
2013
3
5
Caltech Linda Hsieh-Wilson
Caltech
No.18
Deep Caltech
Caltech
1
1
4
Benjamin G.
Davis
3
D2
3
3
1
Ben Davis
Glycobiology
(
)
D3 12
Linda
Linda
25
No.45 (2014 July)
CV
Linda
P. G. Schultz
Linda Slave driver
Caltech
1
1
100
100 °F
37.7
20
30
ESL English as a Second Language
BBQ
26
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MIT
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Caltech
Faculty
Linus Pauling
NMR
$200
Social Party
Linda
Linda Hsieh-Wilson
Fucose-α(1-2)-galactose
(1) Glycosaminoglycan (2) O-GlcNAc
(3)
3
PI(Principal Investigator)
Linda
7
3
9
Linda
9
20-21
1
Micromeeting
Linda
28
Linda
10
18
No.45 (2014 July)
Suggestion
/
O-GlcNAc
Linda
Linda Hsieh-Wilson
Micromeeting
Linda
29
O-GlcNAcylation
No. 45 (2014 July)
第８回 バイオ関連化学シンポジウム
（第 29 回 生体機能関連化学シンポジウム、第 17 回 バイオテクノロジー部会シンポジウム、
第 17 回 生命化学研究会シンポジウム）
http://jointsympo.csj.jp/
2014
9
11
13
3-1-1
JR
15-20
―
7
7
2
18
7
WEB
*
25
http://jointsympo.csj.jp
15
5
3
2
2
40
7
25
5,000
7,000
7
25
2,000
30
3,000
4,000
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*
*
9
12
JR
6,000
Tel : 086-251-8218, Fax: 086-251-8219, E-mail: [email protected]
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No. 45 (2014 July)
第 41 回 国際核酸化学シンポジウム
The 41th International Symposium on Nucleic Acids Chemistry (ISNAC2014)
http://btl.nishitetsutravel.jp/niccs/isnac2014/
2014
11
5
7
3-9-30
10
Harald Schwalbe, Goethe Universitat Frankfurt, Germany
Jean-Jacques Toulme, Bordeaux University, France
Kumiko Ui-Tei, The University of Tokyo, Japan
Mitsuo Sekine, Tokyo Insititute of Technology, Japan
Peter Beal, UC Davis, USA
Ryszard Kierzek, Polish Academy of Sciences, Poland
W. David Wilson, Georgia State University, USA
Wojciech T. Markiewicz, Polish Academy of Sciences, Poland
Yves Mely, CNRS, France
Ta-Chau Chang, Institute of Atomic and Molecular Sciences & Genomics Research Center,
Academia Sinica, Republic of China
Sihyun Ham Sookmyung, Women's University, Korea
Robert H. E. Hudson, The University of Western Ontario, Canada
1. Chemistry of Nucleosides, Nucleotides, and Their Analogues
2. Medicinal Chemistry of Nucleosides and Oligonucleotides
3. DNA/RNA Chemistry and Biochemistry
4. DNA/RNA Structure and Recognition
5. Ribozymes, siRNAs, and miRNAs
6. DNA/RNA Materials and Diagnostics
7. Drug Delivery Systems and Nanotechnology of Oligonucleotides
web site
http://btl.nishitetsutravel.jp/niccs/isnac2014/
2014
6
9
10
15
32
web site
No. 45 (2014 July)
2014
2014
6
6
25,000
5,000
30,000
7,000
9
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web site
15
web site
E-mail:[email protected]
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第 24 回 バイオ・高分子シンポジウム
http://www.spsj.or.jp/entry/
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13:30 15:50
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18:10 20:00
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人工光合成国際会議 2014
2014 International Conference on Artificial Photosynthesis (ICARP2014)
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JST
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20
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20
Purdue Univ. & JST
Richard Cogdell Univ. Glasgow
Univ.
Huub de Groot Univ. Leiden
Sang Ook Kang Korea Univ.
Univ. North Carolina
Devens Gust Arizona State
Can Li Dalian Inst. Chem. Phys.
Daniel Nocera Harvard Univ.
Thomas Meyer
Licheng Sun KTH Royal Inst. Tech.
John Turner Natl. Renewable Energy Lab.
Rienk van Grondelle Free Univ. Amsterdam
Michael Wasielewski Northwestern Univ.
Kyung Byung Yoon Sogang Univ.
40,000
25,000
9
21
ICARP2014
E-mail: [email protected]
http://artificial-photosynthesis.net/ICARP2014/
36
No. 45 (2014 July)
第 17 回 生命化学研究会
∼生命化学の大海原を探る∼
http://res.tagen.tohoku.ac.jp/FBC/
2015
1
8
13
9
780-0862
12
1-3-35
TEL: 088-822-0131
http://www.sansuien.co.jp/
40
720
→
15
200
JR
20
13,000
10,000
200
7,000
E-mail
11/18
80
40
11/18
A4
http://res.tagen.tohoku.ac.jp/FBC/
__
__
558-8585
__
____
3-3-138
E-mail [email protected]
Tel: 06-6605-2696
Fax: 06-6605-2785
37
No. 45 (2014 July)
25
31
2014
2014
1
3
1
924-0838
3-1
E-mail: [email protected]
38
No. 45 (2014 July)
No. 45
45
10
T
…
?
?
S-J
Bern?…
No. 46
2014
10
26
6
20
[email protected]
39
in