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コーニング® スピン・エックス® UF 6 / 20
® For in vitro use only. ® UF 6 / 20 ® ® UF PES UF 6 UF 20 2 mL 6 mL 20 mL No. 5,647,990 UF UF (Page 6) 100 10 30 95% * 1. 25° UF 6 UF 20 15 mL 50 mL 17 mm 30 mm 2. -2- UF 20 (30 mm x 116 mm) 50 mL ** **Beckman Allegra 25R TS-5.1-500 ( Beckman TA-10.250 25° ( ( 75006445 368327) 356966) Heraeus Multifuge 3 S-R 75006441) *** 29 mm x 105 mm *** Sorvall SS34 BUC 5, Beckman JA 20 1. 1/2 2. (Page 4) 3. 4. 5. 3a 3b (Page 5) 1. 2. 3. 4. 99% 1 -3- 1. 5% 25 1.5 50% 2. UF 3. 70% 1. ® * ® UF 6 6 mL 6 mL 20 mL 14 mL 122 mm 17 mm 2.5 cm2 < 10 L 30 L 116 mm 30 mm 6.0 cm2 < 20 L 50 L -4- UF 20 2. UF 6 5,000 100,000 UF 20 5,000 100,000 ® 3a. 20 50,000 4,000 xg 4,000 xg 10,000 xg 6,000 xg 50,000 5,000 xg 3,000 xg 8,000 xg 6,000 xg UF 6 30 %) 25°) 6 mL 6 mL BSA 1.0 mg/mL (66,000 MW) 5,000 10,000 30,000 IgG 0.25 mg/mL (160,000 MW) 30,000 50,000 100,000 ® 3b. 20 20 13 12 98% 98% 98% 12 10 9 98% 98% 97% 18 17 15 96% 96% 91% 15 14 12 95% 95% 91% UF 20 30 %) 25°) 20 mL 20 mL BSA 1.0 mg/mL (66,000 MW) 5,000 10,000 30,000 IgG 0.25 mg/mL (160,000 MW) 30,000 50,000 100,000 23 16 13 99% 98% 98% 29 17 15 99% 98% 98% 27 27 25 97% 96% 91% 20 22 20 95% 95% 90% -5- 4. n- :2 , pH (25.0%) (10.0%) (10.0%) (5.0%) ( ) (100%) (70%) (1.0%) (10.0%) (5.0%) (70.0%) (100%) (30%) (5.0%) (70%) (6M) (1M) (500 mM) (70%) *1 = pH 1 1 3 3 2 1 3 1 3 2 1 1 3 1 1 1 1 3 3 1 1 1 (5%) (10 mM) (60%) (10.0%) (1.0%) (1.0M) (10%) (100%) (20%) (5.0%) (0.1M) (200 ppm) (1.0%) (5.0%) ( 5.0%) (1.0%) (10%) Tween 20 (0.1%) Triton X-100 (0.1%) (8M) ,2= ,3= -6- 1 1 2 1 2 1 1 2 2 1 1 3 2 1 1 3 3 1 1 1 1 ® ® ® UF 1 431482 431483 431484 431485 431486 431487 431488 431489 431490 431491 Corning® Spin-X® UF UF UF UF UF UF UF UF UF UF 6 6 6 6 6 20 20 20 20 20 6 mL 6 mL 6 mL 6 mL 6 mL 20 mL 20 mL 20 mL 20 mL 20 mL 5,000 10,000 30,000 50,000 100,000 5,000 10,000 30,000 50,000 100,000 CORNING Incorporated 170-0052 1-11-44 Tel: 03-3586-1996 Fax: 03-3586-1291 www.corning.com/lifesciences [email protected] -7- 25 25 25 25 25 12 12 12 12 12 24,000 24,000 24,000 24,000 24,000 16,500 16,500 16,500 16,500 16,500