...

生合成機能の高度異種発現に基づく次世代物質生産(PDF)

by user

on
Category: Documents
13

views

Report

Comments

Transcript

生合成機能の高度異種発現に基づく次世代物質生産(PDF)
9 40
J·¸K¹
º¹‡»¼1½
University of Southern California !
1. "#$%&'
()*+,-#./01234567'
()
!"#$
9klm17nopqrstu vwrst
%&'()*+% ,+-.%/
u xuyrstu7z12{(| }~ 1 :
0 & 12345
/;€#9:/`&%=‚=7+G% 8
(5%/678#9:+;<=0 >
)*<Aƒ„+7%_/0 8…=†;ƒ„‡&OU
?@&A
BC 3 D+E;
%Gƒ„+ˆa/+ L‰&7U>#122
(#!"##!" (2) $F
>(GJf6g7h9?=%/0 >(G&
G#HIJ%G (3) &87#!"
@17g7h g7hŠ‹pŒ!" Žl
# >#$FG'&KLM(GN;
 1 ‘’&“=UAJ0 ”•– opqrs
G&O0 P >&()*+
tu vwrstu7z—˜™‹š›Œs
0 +Q" !"(5RS (!"+T,
Gf6 (type I polyketide synthase; type I PKS), xuyr
U/ -'+V; .P#7W+0
stu7zBœžŸA* ¡¢
=/ 8+67$FGX/&0Y#1J
(nonribosomal peptide synthetase, NRPS) &OU(G%
Z'7?@[07&;; %&\]&2J
>+£%/0 PKS NRPS &OU(G%
3^94_0 /` >&a(5b
C6¤¥¦D§Gf6&E¨/F©F8ª«+¬G
5+c;d`e X:(Gf6g7h
J0 PKS 9 type I PKS —˜™­ }®¯
iD5
&O(5&2Jj8+'=U0
f6)*#9°(G#Q±47f6 +²P
0 (1)
Gf6
7*+?H0 —˜™­&³I ´su›µ
8 1 š›Œs Gf6eO¶BœžŸA* ¡¢ Gf6&OU(G%$8
²³´µ¶·¸
¹º»TU¼῎³½
(KS), (AT), ῌ 41
bAB
(ACP) 3 !
Z\KMNO
zefP! {῍ R9:2|MNO
"#$%&'()-CoA *+,-.$%&/)-
}~! €*+,~\‚&+u! TUDEƒD_`„
CoA, 0/)-CoA 1234! 562-.78
…CEGHI2†‡ˆ:wgDEg‰8Zxy9HJ
9:;<=>48 ?@ 2aAB CDEFGHIJK! LMN
KŠ‹&Œg‰8B Ž PKS gPZ R‘Ž’ >
OPQ=R6.*+,Q=R῍STUVW2XY7
48B Z+9:“‘ef2”&•–78&P! —˜™
8Z[K\>48B ]^! NRPS type I PKS _`&!
š•›œ῍ Rdž2348Ÿῌ gh8B
MNOabJK8cdefgh8B MNOP
Type I PKS *+, NRPS ]|MNOP 4¡5 kb ijklD78mbno ! ijk2VW >!
—˜™¢£d‰! ¤K&ab78ƒD¥¦1
lD (C), p)T (A), 0(qr (T) 3 gh8R§! ¨©JKQª«’P¬­gh8®‚K\>4
st&+uivDwExy2st78 ?@ 2
RB
J !
῍ 2 (a) Type I PKS &*o8V-.xyB (b) NRPS &*o8¯06-.xy
KS, °± AT, ± ACP, ± C, lD± A, p)T
± T, 0(qrB
42
2.
´3µo¶·4¸¹
NRPS de novo º¸V»¼½
› "`a&'()œ! r
z"5 16 cžhŸ
type I PKS, NRPS ()6 ¡8GD 6 ‡R
! "
5˜ZU()6E¢ £¤
#$%&'() #*+&'(),-. "/0%)123
5!F_D ¥J˜ .*
$456789(:;<=0) >? 1@ A78
"`a&'()¦§A¨+h©AC
BCD 6E68A FGHI
D 85A i',
JAKCL MNOPQRS12
œ 5Aª-C5F.'C
RSE8T UL de novo 6!GD V
D
8!WX(3X0YZU[\][I56^_78
V8! «¬ª-­/6G"`a&'(
"`a&'()6 b 16 c
)e6D !Œ0®¯h›°
d6 de novo e6
fghD
i Streptomyces lasaliensis LO"`a&'
()=j$kl!T 40 kb mG
5nD o58p
qBr;sat !u]vw6xyE8 "
`a&'()z"? 3 {6|r6D
NRPS A Y}')5~Gu]{h Ecm1 `
a`#)-2-€‚)tA;W:oƒ ;(`„#
;X%…') (FabC) 5;#l0†"$…‡R#ˆ.‰
hD ŠG NRPS C Ecm6 12 7 3X0Y‹!? 4a {hŒL$%.o12
o 8to‡R Ecm17 LŽ$*YxA‰
4#†$0) A &D 4#†$0
) A !}0o‡R5‘ Ecm18 ’ S-}0
oƒ $“:”•'#Yz'60†;–klA
5—˜8 >? 4b@D
iAU$Š™šz"5™šz"!
4 (a) 3X0Y‹$%.o12orŒD (b)
0†;–klrŒ
3 " `a&'()rTz"
FabC, ±1t;(`„#;X%…')² E, ]oY}')² M, }0H2³Y}')D
’“”¹ºE•
»–¼€—˜“½
ῐ 43
ῑ 5 />?Ž
(a) m!mšeA-2-WfA…56/+ (b) ?6/+ (c) NRPS 6/+ sfp,
³³´A11µ«‘¶?6· ecm16, J6· N, NdeI; R, EcoRI; ¸, XbaI/SpeI; X, XhoI.
{T#U|7*Elm!n0A de novo 5}
!"#$%&'(
E'VW
+ E~X]9# €‚ Y
)*+ ,
-.-/012#3 4
2006 Z 9 ƒ„…[7*r†#\‡-
) ˆb
5678#/9:;<=
J`
‰ŠJ)7+
>?" 0@A#B C 5D+ E
F
ῌ῏῎῍
&9 GC G 'HIJE!"KL
OP.-)Fˆ] .<-
J‹^€5ŒF
+ , 9 F > ? NdeI (CATATG), EcoRI
_r5'`Ž#' ca8bOP
(GAATTC), XbaI (TCTAGA), SpeI (ACTAGT) #$%M'
>%J‡‰ _r5)ŒFPZ'IJ ‘)
.&'J+ '%56NO(
7*c’'dJ I:K$“e'E”
*J%fg
9) NdeI #B*PA+ EcoRI #,-PA+
'\*+ bh Œ'•iOP5?6'
. QR9#G;/#.7*E'
j=
&–MN(])/j*E
)*+ / ST-0% XbaI, ')
‰ /'_]$F*.(5H#<*E
U-V0% SpeI #1 XbaIῌSpeI #W
'[3*)\v~+ k‡‰—lFMN'•i)\*
XYZ7*/#.7*+ XbaI SpeI 2%
' l˜™š"›A 743 C 1D _~—l.-mn
M'" 0@A[3)\] J%^" 0@A7*
*OPFo
œ#$z$'G
*+
4>?_%2
JE#56 QR9
,
-56'W-.
‰ ,/_*€
#G^` XbaIῌSpeI WXYZ#a]7"
5Hp'q.
*)\v~+ e€5
0@A7
*E) 899#b:/7*E')*
œGF-o
rž<\*JFs.6t
c;'[3%+ <-
/Fd9
Ÿ#"u_r5uca#v=j w¡¢r
)ST efg2h5%Mi_3U
x*y5'J+ $v£1_]5?#I'
#hjJ*) 786#k=/j*
¤v)
‰ z¥6#&–?@)/
E')*+
j*E_% OPF&'J4"u.%
- E
-b>._%5[3%lm
{1T#H7*E$(*+ - ¦=[
!n"0AF ?@)\*&)A#B7 E
3%x)\*Tݤ)c
* PKS NRPS –
#oC7*E'DE)\%+ bFpGH&FI
?y5 T›§#|¨u95tj*E
-'HpG)q-.Jrc#$s5tj
_] }~OP©OP#.€7*E$[3)
J*E'K-
J*' lm!n"0A56
\*+ QR5ª‚ƒ#
=‡] „…«
$J6L=
*6#uJ`7E
¬0«†­1–#®~E) ¯3«M‡}ˆ
')+ ,E)E6$5tj&1
'OPF£*{1T5$)*°‰
*+ _
Ev <-
&MN_]lm!n"0A'w
r5Š±?5‹bzF J%^5
#
+ '%/
5x 16 Q5?FO
012'c;
‰ $FjŒK$“e#DE
P&'7*5?Qyr3#$zE &R)S
7*E 9²MN7*`)E
-‹^€5#<
ῌ 44
¶{·B!kl¸
¹lº»¼½
>g'op
7)qWz XY&!&!Z{!IJT_`
!"#$%&'(%
*| }~ b&!_`*€~‚%ƒ
)%*+,-./01234
„q=…) †iVD‡ˆ‰‚%oW!*
56778 97:;*<=>1?
Š‹1ƒ„q=…) IJ>7Œ
@A*B*8C
DE*4/
Ži>Hop
‘’“”•ev–i7
FGH
Wp')qW —˜™NOš›&! Chaitan S. Khosla _`
œ—ž™Ÿ™&! ¡¢£™¤_` Charles Richard
IJ KLMNOPQR&!!ST!T%UVW
Hutchinson ¥¦ §š¨š›&! Christopher T. Walsh _
*% )W
XY&!&!Z!IJZ*[\]^_`*
` ©ªœ™&! David E. Cane _` «Ÿ™¬™&!_
abIJ%) [\_`c8!Sdefgeh
` Heinz G. Floss ¥¦‹1tu„q=…) ­® e
ij klemnop
7)qW
¯°i7Wp')qW±G²³*´~µoƒ„q=…
rstu*viw7Wq) ci*1xIJy
)
Fly UP